Německo-české vztahy v Krupce ve 30.letech 20.století

Tato diplomová práce je věnována německo-českým vztahům v Krupce ve 30. letech 20. století. Teoretická část poskytuje informace o situaci v Československu a Sudetech ve 30. letech minulého století a informace o historii města Krupka vycházející z rešerší krupské kroniky. Praktická část je věnována problematice německo-českých vztahů v Krupce, které jsou mapovány prostřednictvím dotazníkového šetření a interview, do nichž byli zapojeni pamětníci. Závěr přináší zhodnocení rozdílů v postavení Čechů a Němců v Krupce ve 30. letech 20. století.The present thesis is concerned with Czech-German relations in Krupka in the 1930s. The theoretical part presents a historical overview of the situation in Czechoslovakia and the Sudetenland. Drawing upon the chronicle of the town of Krupka, it also provides information on the town's history. The empirical part of the thesis deals with Czech-German relations in Krupka. Data was collected through questionnaires and interviews conducted with survivors. The conclusion provides an evaluation of status differences between Czechs and Germans in Krupka in the 1930s.Institute of Germanic StudiesÚstav germánských studiíFilozofická fakultaFaculty of Art

The assessment of existing structure

Cílem tohoto dokumentu je posouzení existující železobetonové skeletové konstrukce jednopodlažní haly. V této práci je postupně proveden statický výpočet a posouzení jednotlivých nosných prvků železobetonové konstrukce. Důležitým aspektem při stanovování zatížení bylo plánované osazení mostového jeřábu s větší nosností. Hlavním podkladem pro posouzení existující konstrukce byl stavebně-technický průzkum, z jehož výsledků byly stanoveny vstupní výpočtové parametry. Cílem práce bylo posoudit, zda je možno při stávajícím technickém stavu konstrukce osadit v budoucnu mostový jeřáb o vyšší požadované nosnosti, případně navrhnout taková technická opatření, která by toto umožňovala.This document aims to assess existing reinforced concrete construction od single-storey hall. There is gradually made static calculation and made judgment on individual supporting elements of reinforced concrete construction. There was very important aspect of assesment load. That aspect was to calculate with planned instalation of a new overhead crane with greater capacity. The main basis for the assessment of existing construction was building-technical survey, the results of which were determined input parameters calculation. The aim of the study was to assess whether it is possible at the current technical state of the construction fitted in the future of higher overhead crane capacity required, or propose such technical measures that would allow this.

Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain

A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA was based on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37.3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed. The variants of arp and tpr genes appear to combine independently with sequence variants of TP0136, TP0548 and 23S rRNA genes

Genome Analysis of Treponema pallidum subsp. pallidum and subsp. pertenue Strains: Most of the Genetic Differences Are Localized in Six Regions

The genomes of eight treponemes including T. p. pallidum strains (Nichols, SS14, DAL-1 and Mexico A), T. p. pertenue strains (Samoa D, CDC-2 and Gauthier), and the Fribourg-Blanc isolate, were amplified in 133 overlapping amplicons, and the restriction patterns of these fragments were compared. The approximate sizes of the genomes investigated based on this whole genome fingerprinting (WGF) analysis ranged from 1139.3–1140.4 kb, with the estimated genome sequence identity of 99.57–99.98% in the homologous genome regions. Restriction target site analysis, detecting the presence of 1773 individual restriction sites found in the reference Nichols genome, revealed a high genome structure similarity of all strains. The unclassified simian Fribourg-Blanc isolate was more closely related to T. p. pertenue than to T. p. pallidum strains. Most of the genetic differences between T. p. pallidum and T. p. pertenue strains were accumulated in six genomic regions. These genome differences likely contribute to the observed differences in pathogenicity between T. p. pallidum and T. p. pertenue strains. These regions of sequence divergence could be used for the molecular detection and discrimination of syphilis and yaws strains

Sesquiterpenes α-humulene and β-caryophyllene oxide enhance the efficacy of 5-fluorouracil and oxaliplatin in colon cancer cells

The present study is designed to find out if sesquiterpenes, α-humulene (HUM), valencene (VAL), β-caryphyllene-oxide (CAO) and trans-nerolidol (NER), are able to improve the antiproliferative effect of classical cytostatic drugs, 5-fluorouracil (FU) and oxaliplatin (1,2-diaminocyclohexaneoxalato-platinum, OxPt), in colon cancer cell lines Caco-2 and SW-620. In addition, the possible mechanisms of sesquiterpene action are studied. The results show significant ability of HUM and especially of CAO to enhance the antiproliferative effects of FU and OxPt in cancer cell lines Caco-2 and SW-620. On the other hand, VAL and NER are ineffective. The action of CAO could be partly based on its ability to disrupt the mitochondrial membrane potential and to activate initiator caspases, but other mechanisms are probably also involved. Based on these results, CAO seems to have the potential for combination therapy of colon cancers and deserves further study

Expression of COBLL1 encoding novel ROR1 binding partner is robust predictor of survival in chronic lymphocytic leukemia

Chronic lymphocytic leukemia is a disease with up-regulated expression of the transmembrane tyrosine-protein kinase ROR1, a member of the Wnt/planar cell polarity pathway. In this study, we identified COBLL1 as a novel interaction partner of ROR1. COBLL1 shows clear bimodal expression with high levels in chronic lymphocytic leukemia patients with mutated IGHV and approximately 30% of chronic lymphocytic leukemia patients with unmutated IGHV. In the remaining 70% of chronic lymphocytic leukemia patients with unmutated IGHV, COBLL1 expression is low. Importantly, chronic lymphocytic leukemia patients with unmutated IGHV and high COBLL1 have an unfavorable disease course with short overall survival and time to second treatment. COBLL1 serves as an independent molecular marker for overall survival in chronic lymphocytic leukemia patients with unmutated IGHV. In addition, chronic lymphocytic leukemia patients with unmutated IGHV and high COBLL1 show impaired motility and chemotaxis towards CCL19 and CXCL12 as well as enhanced B-cell receptor signaling pathway activation demonstrated by increased PLCγ2 and SYK phosphorylation after IgM stimulation. COBLL1 expression also changes during B-cell maturation in non-malignant secondary lymphoid tissue with a higher expression in germinal center B cells than naïve and memory B cells. Our data thus suggest COBLL1 involvement not only in chronic lymphocytic leukemia but also in B-cell development. In summary, we show that expression of COBLL1, encoding novel ROR1-binding partner, defines chronic lymphocytic leukemia subgroups with a distinct response to microenvironmental stimuli, and independently predicts survival of chronic lymphocytic leukemia with unmutated IGHV

Women and employment: advances and challenges in policies for women's labour-force participation in Europe, Latin America and the Caribbean in the context of the post-pandemic recovery

The purpose of this document is to provide inputs and contribute to reflection for decision-making in the field of equality in employment between men and women. It aims to be a document of proposals and measures of public policies that can be taken into consideration, both in the 27 countries of the European Union (EU27) and in their relationship with their partners in Latin America and the Caribbean (LAC), to increase women's participation into labour market in a stable and equitable manner.Fil: Daza Aramayo, LourdesFil: Křížková, AlenaFil: López Igual, PurificaciónFil: Černohorská, Vanda MaufraFil: Nyklová, BlankaFil: Partida Rocha, RaquelFil: Peterson, ElinFil: Pospíšilová, MarieFil: Rocha Carpiuc, CeciliaFil: Rodríguez-Modroño, PaulaFil: Távora, IsabelFil: Vohlidalová, MartaFil: Actis Di Pasquale, Eugenio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Económicas y Sociales; Argentina.Fil: Addabbo, TindaraFil: Aspiazu, Eliana. Universidad Nacional de Mar del Plata. Facultad de Ciencias Económicas y Sociales; Argentina.Fil: Cutuli, Romina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Económicas y Sociales; Argentina.Fil: Damiani, FilippoFil: Daza Aramayo, Lourdes GabrielaFil: Dudová, RadkaFil: Espinosa Fajardo, JuliaFil: Fernández Marín, Ana MaríaFil: Formánková, LenkaFil: Hermanová, Mari

Personalised progression prediction in patients with monoclonal gammopathy of undetermined significance or smouldering multiple myeloma (PANGEA): a retrospective, multicohort study

BACKGROUND: Patients with precursors to multiple myeloma are dichotomised as having monoclonal gammopathy of undetermined significance or smouldering multiple myeloma on the basis of monoclonal protein concentrations or bone marrow plasma cell percentage. Current risk stratifications use laboratory measurements at diagnosis and do not incorporate time-varying biomarkers. Our goal was to develop a monoclonal gammopathy of undetermined significance and smouldering multiple myeloma stratification algorithm that utilised accessible, time-varying biomarkers to model risk of progression to multiple myeloma. METHODS: In this retrospective, multicohort study, we included patients who were 18 years or older with monoclonal gammopathy of undetermined significance or smouldering multiple myeloma. We evaluated several modelling approaches for predicting disease progression to multiple myeloma using a training cohort (with patients at Dana-Farber Cancer Institute, Boston, MA, USA; annotated from Nov, 13, 2019, to April, 13, 2022). We created the PANGEA models, which used data on biomarkers (monoclonal protein concentration, free light chain ratio, age, creatinine concentration, and bone marrow plasma cell percentage) and haemoglobin trajectories from medical records to predict progression from precursor disease to multiple myeloma. The models were validated in two independent validation cohorts from National and Kapodistrian University of Athens (Athens, Greece; from Jan 26, 2020, to Feb 7, 2022; validation cohort 1), University College London (London, UK; from June 9, 2020, to April 10, 2022; validation cohort 1), and Registry of Monoclonal Gammopathies (Czech Republic, Czech Republic; Jan 5, 2004, to March 10, 2022; validation cohort 2). We compared the PANGEA models (with bone marrow [BM] data and without bone marrow [no BM] data) to current criteria (International Myeloma Working Group [IMWG] monoclonal gammopathy of undetermined significance and 20/2/20 smouldering multiple myeloma risk criteria). FINDINGS: We included 6441 patients, 4931 (77%) with monoclonal gammopathy of undetermined significance and 1510 (23%) with smouldering multiple myeloma. 3430 (53%) of 6441 participants were female. The PANGEA model (BM) improved prediction of progression from smouldering multiple myeloma to multiple myeloma compared with the 20/2/20 model, with a C-statistic increase from 0·533 (0·480-0·709) to 0·756 (0·629-0·785) at patient visit 1 to the clinic, 0·613 (0·504-0·704) to 0·720 (0·592-0·775) at visit 2, and 0·637 (0·386-0·841) to 0·756 (0·547-0·830) at visit three in validation cohort 1. The PANGEA model (no BM) improved prediction of smouldering multiple myeloma progression to multiple myeloma compared with the 20/2/20 model with a C-statistic increase from 0·534 (0·501-0·672) to 0·692 (0·614-0·736) at visit 1, 0·573 (0·518-0·647) to 0·693 (0·605-0·734) at visit 2, and 0·560 (0·497-0·645) to 0·692 (0·570-0·708) at visit 3 in validation cohort 1. The PANGEA models improved prediction of monoclonal gammopathy of undetermined significance progression to multiple myeloma compared with the IMWG rolling model at visit 1 in validation cohort 2, with C-statistics increases from 0·640 (0·518-0·718) to 0·729 (0·643-0·941) for the PANGEA model (BM) and 0·670 (0·523-0·729) to 0·879 (0·586-0·938) for the PANGEA model (no BM). INTERPRETATION: Use of the PANGEA models in clinical practice will allow patients with precursor disease to receive more accurate measures of their risk of progression to multiple myeloma, thus prompting for more appropriate treatment strategies. FUNDING: SU2C Dream Team and Cancer Research UK

Mujeres y empleo: avances y desafíos en las políticas para la participación laboral de las mujeres en Europa, América Latina y Caribe en el contexto de la recuperación post-pandémica

El presente documento tiene como finalidad aportar insumos y contribuir a la reflexión para la toma de decisiones en el ámbito de la igualdad en el empleo entre hombres y mujeres. Pretende ser un documento de propuestas y medidas de políticas públicas que podrán tomarse en consideración, tanto en los 27 países de la Unión Europea (UE27) como en su relación con sus socios de América Latina y el Caribe (ALC), para contribuir a una mayor incorporación de mujeres al empleo de manera estable y en condiciones de equidad. Para ello, el documento se ha ordenado en cuatro secciones estructuradas tal y como se expone a continuación.Fil: Daza Aramayo, LourdesFil: Křížková, AlenaFil: López Igual, PurificaciónFil: Černohorská, Vanda MaufraFil: Nyklová, BlankaFil: Partida Rocha, RaquelFil: Peterson, ElinFil: Pospíšilová, MarieFil: Rocha Carpiuc, CeciliaFil: Rodríguez-Modroño, PaulaFil: Távora, IsabelFil: Vohlidalová, MartaFil: Actis Di Pasquale, Eugenio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Económicas y Sociales; Argentina.Fil: Addabbo, TindaraFil: Aspiazu, Eliana. Universidad Nacional de Mar del Plata. Facultad de Ciencias Económicas y Sociales; Argentina.Fil: Cutuli, Romina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Económicas y Sociales; Argentina.Fil: Damiani, FilippoFil: Daza Aramayo, Lourdes GabrielaFil: Dudová, RadkaFil: Espinosa Fajardo, JuliaFil: Fernández Marín, Ana MaríaFil: Formánková, LenkaFil: Hermanová, Mari