Enhanced decolourization of congo red dye under submerged fermentation (SMF) process by newly isolated Bacillus subtilis SPR42

Studies were carried out on the decolourization of textile azo dye using newly isolated aerobic bacterial culture. Among the 58 strains of aerobic bacteria isolated from soil contaminated with textile industry (Shivalik polymer Ltd. Faridabad) effluent, three showed remarkable ability in decolourizing the widely utilized azo dye (Congo Red). These strains also readily grew in and decolourized the high concentrations of dye (100 mgL -1). The aerobic bacterial isolate SPR42, was able to decolourize the Congo Red dye at a concentration of 100 mgL -1 upto 94% within 24 hrs at static conditions. The temperature and pH for optimum growth and activity of the isolate were reported as 37 oC and 8.5, respectively. The colorless bacterial biomass after decolourization suggested that decolourization was due to biodegradation, rather than inactive surface adsorption. Phenotypic characterization and phylogenetic analysis based on 16S rDNA sequence comparisons indicate that the strain SPR42 identified as Bacillus subtilis. This isolate can be a potential strain for biological treatment of effluents of TPI (Textile Processing Industry)

Loop-mediated isothermal amplification (LAMP) based detection of bacteria: A Review

Various diseases are caused by pathogenic bacteria and their diagnosis depends on accurate detection of pathogen from clinical samples. Several molecular methods have been developed including PCR, Real Time PCR or multiplex PCR which detects the pathogen accurately. However, every method has some limitations like low detection limit, whereas Loop-mediated isothermal amplification (LAMP) is a powerful and novel nucleic acid amplification method, which detects the DNA at very low level compared to other methods. This method amplifies very few copies of target DNA with high specificity, efficiency and rapidity under isothermal conditions by using a set of four specially designed primers and a DNA polymerase with strand displacement activity. This review presents detection of various bacteria by LAMP method and covers their detection limit in clinical specimens.Keywords: Bacteria, Loop-mediated isothermal amplification (LAMP), sensitive, rapid, simple.African Journal of Biotechnology, Vol 13(19), 1920-192

Efficacy of Sulfoxaflor 12% SC against Aphids Complex and Coccinella septempunctata L. in Wheat.

Not AvailableEfficacy of dosages of sulfoxaflor 12%SC were evaluated for the control of foliar aphids under Contract Research Project on wheat during rabi seasons of 2014-15 and 2015-16 at the research farm of ICAR- Indian Institute of Wheat and Barley Research, Karnal, Haryana. The standard check insecticides thiamethoxam 25WDG and quinalphos 25EC were included along with sulfoxaflor, and all the treatments were found to be significantly superior. The incidence of aphids/ shoot/ plant was significantly less with sulfoxaflor 12 SC @ 24, 27 and 30 g a.i./ ha with 94.54, 95.27 and 96.03% reduction, respectively; these being at par with each other, followed by thiamethoxam 25WDG @ 12.5 g a.i./ ha and quinalphos 25EC @ 250 g a.i./ ha. The seven-spotted ladybird beetle Coccinella septempunctata L. was the main natural enemy observed in the crop, and its counts were the least (1.50/ m2) with thiamethoxam 25WDG @ 12.5 g a.i./ha at 14 DAS. The pooled data revealed that the yield was significantly more with sulfoxaflor 12SC @ 24, 27 and 30 g a.i./ ha (52.21, 52.62 and 54.32 q/ ha) followed by thiamethoxam 25WDG @ 12.5 g a.i./ ha (49.87 q/ ha) which was at par with quinalphos 25EC @ 250 g a.i./ ha (47.73 q/ha). Considering incremental cost benefit ratio, sulfoxaflor 12% SC @30 g a.i/ ha (1:2.70) is the most economical, and no phytotoxicity symptoms were observed at its doses @30 and 60 g a.i/ ha

Influence of sowing time and weather factors on seasonal dynamics of aphids in three wheat growing zones of India

Not AvailableA study was conducted to evaluate the relative population abundance of foliar aphid on wheat at three locations (Karnal, Niphad and Kharibari) sown on three different dates during rabi season of 2013-14 and 2014-15.The highest seasonal average population of aphids of 44.5 and 61.5 aphids per tiller at Karnal and Niphad, respectively were recorded on the crop sown during Ist fortnight of November and the lowest seasonal average population of 4.3 and 2.7 aphids per tillerat Karnal and Niphad, respectively were recorded on crop sown during 2nd fortnight of December. The studies indicated that early planted crop during November month provided the congenial weather conditions for aphid proliferation during crop growth period