Modeling 3D Retinogenesis in Mouse Embryonic Stem Cells Following CRISPR-mediated Crx Knockdown

An emerging technology known as three-dimensional (3D) tissue engineering has allowed scientists to mimic tissues found in vivo. Previous studies indicate that it is possible to differentiate dissociated mouse embryonic stem cells (mESCs) into 3D retinal tissues in vitro (Bertacchi, 2015; Eiraku, 2012). The newly differentiated retinal tissues are said to encompass all of the major components found in retinal tissues. The generation of in vitro 3D tissues holds great potential in terms of patient-specific disease modeling. Although various diseases have been well-studied in animal models, there are limitations with regards to patient-specificity. The generation of animal models to study each specific mutation would be impractical and costly. With in vitro generation of tissues and organs, scientists have the capacity to generate disease models consisting of mutations specific to patients. There are various gene editing techniques available to generate and recreate disease-specific 3D disease models using stem cells. The newest gene editing technique known as Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) has allowed scientists to edit the genome with greater precision than other genome editing technologies like Zinc Finger Nucleases (ZFNs) or Transcription Activator-like Effector Nucleases (TALENs). In order to overcome current limitations on disease-specific model systems, we developed a protocol for differentiating mouse embryonic stem cells into 3D retinas in vitro. In addition, to confirm the utility of this 3D model for future disease-specific mutations, we utilized the CRISPR method as a means to knockdown the transcription factor CRX, which is known to regulate key developmental decisions during retinogenesis, and as such, mutations in CRX have resulted in various diseases such as Leber Congenital Amaurosis, Cone-Rod Dystrophy, and Retinitis Pigmentosa

Primary Tuberculosis of Tonsils: A Case Report

Tuberculosis is one of the major causes of ill health and death worldwide. Isolated tuberculosis of tonsil in the absence of active pulmonary tuberculosis is a very rare clinical entity. A 10-year-male child presented with recurrent episodes of upper respiratory tract infections, with 2-3 occurrences per month for the past 6 years. On general physical examination, bilateral tonsils showed grade III enlargement and congestion. Posterior pharyngeal wall was clear. Examination of the chest was within normal limits. Histopathological examination of bilateral tonsils revealed caseating and noncaseating epithelioid cell granulomas with Langhans giant cells. Ziehl-Neelsen stain for acid fast bacillus was positive. Features were consistent with a diagnosis of tuberculosis of tonsils. Tuberculosis of the oral cavity is uncommon and lesions may be either primary or secondary. Early detection and intervention is essential for cure. Isolated and primary tuberculosis of the tonsils in the absence of pulmonary tuberculosis is a rare entity, which prompted us to report this case

Representation zeta functions of arithmetic groups of type A2A_2 in positive characteristic

We prove two conjectures regarding the representation growth of groups of type $A_2$. The first, conjectured by Avni, Klopsch, Onn and Voll, regards the uniformity of representation zeta functions over local complete discrete valuation rings. The second is the Larsen--Lubotzky conjecture on the representation growth of irreducible lattices in groups of type $A_2$ in positive characteristic assuming Serre's conjecture on the congruence subgroup problem.Comment: Preliminary draft, comments are welcomed

PRANIC HEALING: A STRESS BUSTER FOR CANCER PATIENTS.

Pranic healing is a highly developed and tested system of energy based healing techniques that utilizes \"Prana\" to balance harmonize and transform the body\'s energy processes. \"Prana\" is a Sanskrit word that means \"Life-force\" This invisible bio-energy or vital energy keeps the body alive and maintains a state of good health. In acupuncture, the Chinese refer to this subtle energy as \"Chi\". It is also called \"Ruah\" or the \"Breath of life\" in the old testament. Pranic healing is a simple yet powerful and effective no touch energy healing. It is based on the fundamental principle that the body is a \"Self-repairing\" living entity that possesses the innate ability to heal itself. Pranic healing works on the principle that the healing process is accelerated by increasing the life force or vital energy on the affected part of the physical body. Pranic healing influences this natural life force to being about a healthier physical body. Pranic healing is applied on the bio-electromagnetic field known as the aura, which contains the mold and blueprint of the physical body. This bioplasmic body absorbs life energy and distributes it to the organs and glands. Disease first appear as energetic disruption in the energy field before manifesting as ailments in the physical body. With all diseases including cancer the first objective is to understand that the cause is thoughts one\'s own guilt or harmful thoughts and feeling created by one\'s upon himself. To heal from cancer one must fully cleanse the physical, emotional and mental layers of body system

A Review on Pottali Kalpana w.s.r. to Kaparda Purana Putapaka method of preparation

Pottali Kalpana is one of the unique Kalpana mentioned in our classics. It is one among the four Moorchita Parada Yogas. Kaparda Purana Putapaka method is one the methods mentioned for preparation of this Kalpana. It brings in the compactness of the Dravyas. Pottali Kalpana are a blend of extremely important trace elements which form the crux of the immune system. Various Kaparda Purana Method of preparation of Pottalis have been compiled here which have been mentioned in classical Rasashastra texts along with its ingredients and other variations. The alkalinity of the Kaparda Purana method of preparation owing to calcium probably has a significant role to play in the management of Atisara and Grahani. This Kalpana can be used in emergency care as well

Correction to: Studies on H2-Assisted Liquefied Petroleum Gas Reduction of NO over Ag/Al2O3 Catalyst

Correction to: Bulletin of Chemical Reaction Engineering & Catalysis (2018), 13 (2): 227-235 (doi:10.9767/bcrec.13.2.1307.227-235)An error appeared in Corresponding Author in a paper entitled “Studies on H2-Assisted Liquefied Petroleum Gas Reduction of NO over Ag/Al2O3 Catalyst” published in Bulletin of Chemical Reaction Engineering & Catalysis. The Corresponding Author is corrected to be:* Corresponding Authors. Tel: +919415268192. Email: [email protected] (R. Prasad) Tel: +917505072607. Email: [email protected] (D. Yadav)——————The original article can be found online at: https://doi.org/10.9767/bcrec.13.2.1307.227-235——————Copyright © 2020 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).How to Cite: Singh, P., Yadav, D., Thakur, P., Pandey, J., Prasad, R. (2020). Correction to: Studies on H2-Assisted Liquefied Petroleum Gas Reduction of NO over Ag/Al2O3 Catalyst. Bulletin of Chemical Reaction Engineering & Catalysis, 15 (2): 603-603 (doi:10.9767/bcrec.15.2.7659.603-603)Permalink/DOI: https://doi.org/10.9767/bcrec.15.2.7659.603-60

Studies on H2-Assisted Liquefied Petroleum Gas Reduction of NO over Ag/Al2O3 Catalyst

Hydrocarbon-Selective catalytic reduction (HC-SCR) is one of the potential methods to remove NOx emissions from diesel engine, lean burn petrol engines and natural gas engines exhaust. Ag/Al2O3 is a good catalyst for HC-SCR of NOx under lean-burn conditions. Further, addition of small amount of H2 is effective for enhancing HC-SCR activity. This effect is unique to silver and to specific Ag/support combinations, namely, Ag/γ-Al2O3. Various HC reductants, such as: octane, decane, dodecane and propane, have been reported in the literatures. Only a single study on LPG as a reductant over Cu-ZSM catalyst was reported. There was no work reported on H2 assisted LPG over Ag/Al2O3 catalyst. Thus, this gap in the literature is filled with the present investigation of NO reduction over 2 wt.% Ag/Al2O3 catalyst using LPG reductant. The fresh and used catalyst was characterized by various techniques like low temperature N2-adsorption, XRD, XPS and SEM. There was practically no change in the characteristics of the fresh and used catalyst. Two different reductants of CO and LPG were compared for SCR of NO over the catalyst without and with H2-assisted. The experiments were performed in a fixed bed tubular flow reactor under the following conditions: 100mg catalyst; 0.13% NO, 2.5% LPG/CO, 1% H2, 10% O2, rest Ar; total flow rate 60 mL/min; temperature ambient 400 oC and pressure 1 atm. Around 100% conversion of NO was achieved using LPG reductant. Light off temperature of NO reduction significantly reduced by H2assisted LPG reductant. The maximum conversion of NO with CO was limited to 35.15% at temperature of 224 oC and above. Whereas, 97.79 % NO conversion was achieved at 365 oC with LPG reductant. While, the maximum conversions with H2-LPG and H2-CO reductants were 100 and 99.46% at 117 and 220 oC, respectively. Therefore, H2-LPG-SCR of NOx over 2 wt.% Ag/Al2O3 catalyst system can be used to get 100% reduction at low temperature.

UNCERTAINITY PROCESS USING DEPENDABLE PEER TO PEER NETWORKS

One of the key functions of these systems is to solve queries efficiently or find files. This is the problem that is addressed in this document. In organized systems, the files are organized to create overlays with specific topology attributes. It is very difficult to locate a document or resource within an unregulated peer-to-peer network. It has been shown that this approach leads to a stable query load that is affected by information and service restriction, that is, it ensures that the query routes meet the pre-defined limits by category, according to the possibility of linking the search. The deficiencies of partially disorganized systems can be addressed in part by hybrid P2P Systems. Additional aspects associated with the reduction of complexity, the estimation of parameters and the adaptability to query precision based on queries and traffic loads are studied. The function suggests a strategy where colleagues store the final results of previous queries, as shown by redirecting the reverse route. This method includes a huge amount of data and has not yet provided performance guarantees. An explicit image of the capacity area of ​​these systems is provided and numerically co-related with random searches based on hikes