In vivo ispitivanje enzimske aktivnosti nekih Ru(II) jedinjenja sa N-alkilfenotiazinima

The purpose of the present study was to investigate and compare the effects of two ruthenium complexes with trifl uoperazine on acethylcholinesterase enzyme activity and lactate dehydrogenase levels in vivo under physiological conditions in rats blood. Complexes 1 and 2 showed positive effects on acethylcholinesterase at all doses and did not disturb its normal activity. Total LDH activity was inhibited in the presence of both complexes, but Ru(II) complexes showed different effects on the activity of LDH isoenzymes. The activities of LDH1 and LDH2 isoenzymes were decreased in all applied doses of the complex 2, while the activity of LDH2 reduced using complex 1 in the same doses. Results of the present study suggest the neuro-and cardio protective potential of oral administration of complexes 1 and 2, as non-toxic compounds under physiological conditions. These protective effects are the result of their potent antioxidant activity.Cilj ovog rada je da se ispitaju i uporede efekti dva kompleksa rutenijuma sa trifluoperazinom na aktivnost enzima acetilholinesteraze i laktat-dehidrogenase in vivo pod fiziološkim uslovima u krvi pacova. Kompleksi 1 i 2 pokazali su pozitivan efekat na aktivnost acetiholinesteraze u svim primenjenim dozama. Ukupna aktivnost LDH je inhibirana u prisustvu oba kompleksa, ali kompleksi Ru(II) pokazuju različite rezultate na izoenzimske oblike ovog enzima. Aktivnosti izoenzima LDH1 i LDH2 su smanjene u svim primenjenim dozama kompleksa 2, dok kompleks 1 smanjuje aktivnost samo izoenzima LDH2 u tim istim koncentracijama. Rezultati prikazanog istraživanja ukazuju na neuro - i kardio zaštitni potencijal oralne primene kompleksa 1 i 2, kao netoksičnih jedinjenja pod fiziološkim uslovima, indukovano preko njihovog snažnog antioksidativnog efekta

Supplementary data for article: Misirlić Denčić, S.; Poljarević, J.; Isakovic, A. M.; Marković, I.; Sabo, T. J.; Grgurić-Šipka, S. Antileukemic Action of Novel Diamine Pt(II) Halogenido Complexes: Comparison of the Representative Novel Pt(II) with Corresponding Pt(IV) Complex. Chemical Biology and Drug Design 2017, 90 (2), 262–271. https://doi.org/10.1111/cbdd.12945

Supporting information for: [https://doi.org/10.1111/cbdd.12945]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2483]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3120

Supplementary data for article: Poljarević, J.; Grgurić-Šipka, S.; Kaluđerović, G. N.; Sabo, T. Dibromido[(S,S)-Ethylenediamine-N,N ’-Di-2-(3-Cyclohexyl)Propanoato]Platinum(IV): Synthesis, Characterization, and DFT Calculations. Journal of Coordination Chemistry 2011, 64 (6), 1016–1022. https://doi.org/10.1080/00958972.2011.560940

Supplementary material for: [https://doi.org/10.1080/00958972.2011.560940]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1160

Antimicrobial potency of Ru(II) arene based pyridil complexes

Discover a new class of ruthenium-based complexes that were investigated as potential antimicrobial agents: dinuclear polypyridil ruthenium(II) complexes exhibited excellent growth inhibition, and Ru(II) arene complexes with acetyl pyridine ligands exhibited moderate antimicrobial activity in the panel of bacteria1. Here we have synthesized 14 new Ru(II) arene complexes with pyridine-based ligands and examined their antimicrobial potency, trying to correlate their structure and biological activity. Reported complexes were obtained in a reaction of [Ru(η6-benzene)Cl(μ-Cl)]2 or [Ru(η6-toluene)Cl(μ-Cl)]2 with halogen derivatives of picolinic acid or pyridine dicarboxylic acids in a 1:2 molar ratio in ethanol. The complexes were soluble in DMSO and water. Their structural characterization included IR and NMR spectroscopy and MS spectrometry, and purity was confirmed by elemental analysis. In this report, we demonstrate the activities of these novel compounds against six typical gram-negative and two gram-positive bacteria. A micro-well dilution assay was used to determine the minimum inhibitory concentration (MIC), and minimum bactericidal concentration. Streptomycin and chloramphenicol, commercial antibiotics, were used as a positive control. The best activity of all tested bacteria was observed against E. coli, with a MIC value of 1.25 mg/mL, for C3, C6, and C10 complexes. Also, all synthesized complexes showed the same activity against C. albicans

Ru(II) bipiridinski kompleksi sa analozima acetilpiridina: spektralna i elektrohemijska karakterizacija

The versatile chemistry of ruthenium complexes involves thousands of compounds aimed for different applications related to e.g. homogenous catalysis, cancer therapy, tumor diagnosis, and advanced materials.1 Thus, the synthesis and full (electro)chemical characterization of three new Ru(II) complexes carrying acetylpyridine (acpy) ligand unitis is described. The complexes were obtained via reaction of three ligand equivalents (2-, 3-, and 4-acpy) with an equimolar amount of metal precursor, [RuCl2(bpy)2] in methanol. After the overnight reflux, the reaction mixture was left to cool when equimolar amount of NH4PF6 was added. The products were isolated in a form of dark red powder. The complexes were characterized by IR, NMR and MS revealing bidentate coordination of 2- acpy and monodentate binding of 3- and 4-acpy. Their electrochemical profile was studied by cyclic voltammetry which confirmed rich redox chemistry.Raznovrsna hemija kompleksa rutenijuma obuhvata hiljade jedinjenja namenjenih za različite primene, npr. homogenu katalizu, terapiju kancera, dijagnozu tumora i moderne materijale.1 S tim u vezi se opisuje sinteza i kompletna (elektro)hemijska karakterizacija tri nova Ru(II) kompleksa sa acetilpiridinskim ligandom (acpy). Kompleksi su dobijeni reakcijom tri ekvivalenta liganda (2-, 3-, i 4-acpy) sa ekvimolarnom količinom prekursora metala, [RuCl2(bpy)2] u metanolu. Nakon refluksa preko noći, reakciona smeša je ostavljena da se ohladi kad je dodata ekvimolarna količina NH4PF6. Produkti su izolovani u obliku tamnocrvenog praha. Kompleksi su okarakterisani IC, NMR i MS pokazujući bidentatnu koordinaciju 2-acpy i monodentatno vezivanje 3- i 4-acpy. Njihov elektrohemijski profil je ispitan cikličnom voltametrijom koja je potvrdila bogatu redoks hemiju

Supplementary data for the article: Poljarević, J. M.; Tamás Gál, G.; May, N. V.; Spengler, G.; Dömötör, O.; Savić, A. R.; Grgurić-Šipka, S.; Enyedy, É. A. Comparative Solution Equilibrium and Structural Studies of Half-Sandwich Ruthenium(II)(η 6 -Toluene) Complexes of Picolinate Derivatives. J. Inorg. Biochem. 2018, 181, 74–85. https://doi.org/10.1016/j.jinorgbio.2017.12.017

Supplementary material for: [https://doi.org/10.1016/j.jinorgbio.2017.12.017]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2119