Hatchery manual for broodstock management and larval production of tubrot (Psetta maxima)

This hatchery manual is intended to provide detailed information from available published work and grey literature on turbot broodstock management and larval production. In reviewing larviculture techniques for turbot, it is notable that the major initial zoo technical advances were made in the 1980s. Subsequent refinements have been industry-led and are subject to commercial confidentiality. Some actors in the sector that have been approached either did not provide requested information or denied access to their sources of information. This manuscript therefore considers those aspects of commercial rearing techniques that are in the public domain, together with the applied scientific literature and information collected from different experts. With the aim to cover all aspects related to the production of turbot juveniles in Dutch farms from egg to fish of 10-15 g, the manual describes in details different steps. These have been grouped around the broodstock management, the hatchery/nursery period, the on growing and the grow out periods. A list of common diseases in turbot has been added at the end. The manual provides a link to the most update information available on live prey production and enrichment

Microbial symbionts of parasitoids

Parasitoids depend on other insects for the development of their offspring. Their eggs are laid in or on a host insect that is consumed during juvenile development. Parasitoids harbor a diversity of microbial symbionts including viruses, bacteria, and fungi. In contrast to symbionts of herbivorous and hematophagous insects, parasitoid symbionts do not provide nutrients. Instead, they are involved in parasitoid reproduction, suppression of host immune responses, and manipulation of the behavior of herbivorous hosts. Moreover, recent research has shown that parasitoid symbionts such as polydnaviruses may also influence plant-mediated interactions among members of plant-associated communities at different trophic levels, such as herbivores, parasitoids, and hyperparasitoids. This implies that these symbionts have a much more extended phenotype than previously thought. This review focuses on the effects of parasitoid symbionts on direct and indirect species interactions and the consequences for community ecology

Classic Infantile Pompe disease: Effects of dosing and immunomodulation on long-term outcome

Pompe disease, glycogen storage disease type II (GSD II), glycogenosis type II, and acid maltase deficiency (OMIM #232300) are all names used for the same rare autosomal recessive disease, which is the subject of this thesis. Until recently this was a deadly disease in infants and severely invalidating in children and adults. The development of enzyme replacement therapy (ERT) has brought new prospects for patients and their families. This thesis will provide information on the historical background of Pompe disease,genetics and inheritance, diagnosis and treatment. The current treatment options are discussed with a specific focus on the effects of enzyme replacement therapy, dosing and immunomodulation, in patients and how these developments have led to the publications in this thesis

Population estimation for the Urban Atlas Polygons

The aim of this technical note is to describe the methodology and source data used to estimate the residential population in each built-up polygon of the Urban Atlas land use/cover dataset. The final outcome of the procedure is a new attribute to the Urban Atlas polygons that will broaden the range of uses of the Urban Atlas dataset, contributing to new analysis and assessments in different thematic fields, e.g. urban quality of life (accessibility to recreational areas; exposure to sources of noise); urban morphology (population density gradients).JRC.H.8-Sustainability Assessmen

Hyperparasitoids exploit herbivore-induced plant volatiles during host location to assess host quality and non-host identity

Although consumers often rely on chemical information to optimize their foraging strategies, it is poorly understood how top carnivores above the third trophic level find resources in heterogeneous environments. Hyperparasitoids are a common group of organisms in the fourth trophic level that lay their eggs in or on the body of other parasitoid hosts. Such top carnivores use herbivore-induced plant volatiles (HIPVs) to find caterpillars containing parasitoid host larvae. Hyperparasitoids forage in complex environments where hosts of different quality may be present alongside non-host parasitoid species, each of which can develop in multiple herbivore species. Because both the identity of the herbivore species and its parasitization status can affect the composition of HIPV emission, hyperparasitoids encounter considerable variation in HIPVs during host location. Here, we combined laboratory and field experiments to investigate the role of HIPVs in host selection of hyperparasitoids that search for hosts in a multi-parasitoid multi-herbivore context. In a wild Brassica oleracea-based food web, the hyperparasitoid Lysibia nana preferred HIPVs emitted in response to caterpillars parasitized by the gregarious host Cotesia glomerata over the non-host Hyposoter ebeninus. However, no plant-mediated discrimination occurred between the solitary host C. rubecula and the non-host H. ebeninus. Under both laboratory and field conditions, hyperparasitoid responses were not affected by the herbivore species (Pieris brassicae or P. rapae) in which the three primary parasitoid species developed. Our study shows that HIPVs are an important source of information within multitrophic interaction networks allowing hyperparasitoids to find their preferred hosts in heterogeneous environments

Exploiting chemical ecology to manage hyperparasitoids in biological control of arthropod pests

Insect hyperparasitoids are fourth trophic level organisms that commonly occur in terrestrial food webs, yet they are relatively understudied. These top-carnivores can disrupt biological pest control by suppressing the populations of their parasitoid hosts, leading to pest outbreaks, especially in confined environments such as greenhouses where augmentative biological control is used. There is no effective eco-friendly strategy that can be used to control hyperparasitoids. Recent advances in the chemical ecology of hyperparasitoid foraging behavior have opened opportunities for manipulating these top-carnivores in such a way that biological pest control becomes more efficient. We propose various infochemical-based strategies to manage hyperparasitoids. We suggest that a push-pull strategy could be a promising approach to ‘push’ hyperparasitoids away from their parasitoid hosts and ‘pull’ them into traps. Additionally, we discuss how infochemicals can be used to develop innovative tools improving biological pest control (i) to restrict accessibility of resources (e.g. sugars and alternative hosts) to primary parasitoid only or (ii) to monitor hyperparasitoid presence in the crop for early detection. We also identify important missing information in order to control hyperparasitoids and outline what research is needed to reach this goal. Testing the efficacy of synthetic infochemicals in confined environments is a crucial step towards the implementation of chemical ecology-based approaches targeting hyperparasitoids. © 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry

Projectrapportage Cooperatief pootvis productiecentrum voor de Nederlandse mariene viskweek sector

Het doel van dit project is het demonstreren van de haalbaarheid van een coöperatief productiecentrum voor tarbot en tong pootvis ten behoeve van de Nederlandse platviskweek sector. De rapportage gaat voornamelijk in op de kennisvragen m.b.t. de kosten en de technische haalbaarheid van een pootvis productiesysteem

Genotypic variation in genome-wide transcription profiles induced by insect feeding: Brassica oleracea – Pieris rapae interactions

<p>Abstract</p> <p>Background</p> <p>Transcriptional profiling after herbivore attack reveals, at the molecular level, how plants respond to this type of biotic stress. Comparing herbivore-induced transcriptional responses of plants with different phenotypes provides insight into plant defense mechanisms. Here, we compare the global gene expression patterns induced by <it>Pieris rapae </it>caterpillar attack in two white cabbage (<it>Brassica oleracea </it>var. <it>capitata</it>) cultivars. The two cultivars are shown to differ in their level of direct defense against caterpillar feeding. Because <it>Brassica </it>full genome microarrays are not yet available, 70-mer oligonucleotide microarrays based on the <it>Arabidopsis thaliana </it>genome were used for this non-model plant.</p> <p>Results</p> <p>The transcriptional responses of the two cultivars differed in timing as characterized by changes in their expression pattern after 24, 48 and 72 hours of caterpillar feeding. In addition, they also differed qualitatively. Surprisingly, of all genes induced at any time point, only one third was induced in both cultivars. Analyses of transcriptional responses after jasmonate treatment revealed that the difference in timing did not hold for the response to this phytohormone. Additionally, comparisons between <it>Pieris rapae</it>- and jasmonate-induced transcriptional responses showed that <it>Pieris rapae </it>induced more jasmonate-independent than jasmonate-dependent genes.</p> <p>Conclusion</p> <p>The present study clearly shows that global transcriptional responses in two cultivars of the same plant species in response to insect feeding can differ dramatically. Several of these differences involve genes that are known to have an impact on <it>Pieris rapae </it>performance and probably underlie different mechanisms of direct defense, present in the cultivars.</p

Understanding insect foraging in complex habitats by comparing trophic levels: insights from specialist host-parasitoid-hyperparasitoid systems

Insects typically forage in complex habitats in which their resources are surrounded by non-resources. For herbivores, pollinators, parasitoids, and higher level predators research has focused on how specific trophic levels filter and integrate information from cues in their habitat to locate resources. However, these insights frequently build specific theory per trophic level and seldom across trophic levels. Here, we synthesize advances in understanding of insect foraging behavior in complex habitats by comparing trophic levels in specialist host-parasitoid-hyperparasitoid systems. We argue that resources may become less apparent to foraging insects when they are member of higher trophic levels and hypothesize that higher trophic level organisms require a larger number of steps in their foraging decisions. We identify important knowledge gaps of information integration strategies by insects that belong to higher trophic levels