The Efficacy of Permot (Passiflora Foetida Linn.) Leaves Crude Extract Ointment on the Healing of Skin of Rabbit with Scabies

This research aimed to know the efficacy of active compound of  Permot (P. foetida) leaves crude extract ointment as a bioacaricide in rabbit through observation of after treatment histopathological changes on skin of body part naturally infested with Sarcoptes scabiei. Permot  leaves were isolated and extracted in n-hexane solvent. Sixteen rabbits were randomly  divided into four groups, control (P0), P1, P2 and P3, the group of rabbits with scabies treated respectively with 5, 10 and 15 % of permot leaves crude extract ointment. Therapy was conducted topically on day 0; 1; 2; 3; 4; 5. Efficacy was examined from Haematoxylin Eosin (HE) stained skin histopathological features under microscope with  magnification of 100x, 400x and 1000x. The obtained skin histopatological feature data were assessed qualitatively. The application of 5 % ointment (P1) showed erosion and severe inflammatory cells infiltration in the epidermis; follicle proliferation and sebaceous were not present. In the dermis, severe sebaceous adenitis and folliculitis were evident. Massive inflammatory cells infiltration was found in hypodermis. The therapy using 5% ointment resulted 45 % success rate. The application of 10 % permot leaves crude extract ointment (P2) showed severe hypergranulation in the epidermis, severe proliferation of follicle and sebaceous gland, mild sebaceous adenitis and mild folliculitis in the dermis, as well as  moderate inflammatory cells infiltration in the hypodermis. The therapy using 10,% ointment resulted 60 % success rate. The application of 15 % ointment (P3) showed mild hypergranulation in the epidermis, mild follicle proliferation and inflammatory cells (PMN) infiltrationin the dermis; and moderate inflammatory cells infiltration in the hypodermis. The therapy using 15 % ointment resulted 90 % success rate. Keywords: scabiosis, rabbits, Passiflora foetida, ointment, histopatholog

Diversitas Genetik Eimeria spp., yang Menginfeksi Sapi Madura

Surat Penerima Pendanaan Penelitian dari Kementerian Riset dan Teknologi / BRIN Tahun 2020 dengan judul " Diversitas Genetik Eimeria spp., yang Menginfeksi Sapi Madura

Pidato Pengukuhan dengan Judul Scabies Penyakit Zoonosis yang Terabaikan dan Pengembangan Pengendaliannya dengan Tanaman Permot sebagai Bioacarisida

Scabies sebagai Masalah Kesehatan Global Scabies atau lebih dikenal sebagai kudis adalah penyakit kulit menular yang disebabkan oleh tungau (mites) Sarcoptes scabiei. Tungau Sarcoptes telah dilaporkan menginfestasi lebih dari 100 spesies mamalia termasuk manusia dan hewan domestik. Scabies pada manusia disebabkan oleh Sarcoptes scabiei var. hominis telah menginfestasi lebih dari 300 juta orang penduduk setiap tahun (WHO, 2014). Perkiraan prevalensi scabies berkisar antara 0,2% hingga 71% (WHO, 2020). Scabies termasuk dalam emerging/ re-emerging parasitic disease yang dapat mengancam kesehatan hewan dan manusia di dunia, penularan dapat terjadi dari hewan ke manusia hingga termasuk ke dalam daftar penyakit zoonosis (Alasaad et al., 2013)

The Efficacy of Permot (Passiflora foetida Linn.) leaves Crude Extract Oinment on The Healing of Skin of Rabbit wth Scabies

This research aimed to know the efficacy of active compound of Permot (P. foetida) leaves crude extract ointment as a bioacaricide in rabbit through observation of after treatment histopathological changes on skin of body part naturally infested with Sarcoptes scabiei. Permot leaves were isolated and extracted in n-hexane solvent. Sixteen rabbits were randomly divided into four groups, control (P0), P1, P2 and P3, the group of rabbits with scabies treated respectively with 5, 10 and 15% of permot leaves crude extract ointment. Therapy was conducted topically on day 0; 1; 2; 3; 4; 5. Efficacy was examined from Haematoxylin Eosin (HE) stained skin histopathological features under microscope with magnification of 100x, 400x and 1000x. The obtained skin histopatological feature data were assessed qualitatively. The application of 5% ointment (P1) showed erosion and severe inflammatory cells infiltration in the epidermis; follicle proliferation and sebaceous were not present. In the dermis, severe sebaceous adenitis and folliculitis were evident. Massive inflammatory cells infiltration was found in hypodermis. The therapy using 5% ointment resulted 45% success rate. The application of 10% permot leaves crude extract ointment (P2) showed severe hypergranulation in the epidermis, severe proliferation of follicle and sebaceous gland, mild sebaceous adenitis and mild folliculitis in the dermis, as well as moderate inflammatory cells infiltration in the hypodermis. The therapy using 10,% ointment resulted 60% success rate. The application of 15% ointment (P3) showed mild hypergranulation in the epidermis, mild follicle proliferation and inflammatory cells (PMN) infiltrationin the dermis; and moderate inflammatory cells infiltration in the hypodermis. The therapy using 15% ointment resulted 90% success rate. Keywords: scabiosis, rabbits, Passiflora foetida, ointment, histopatholog

Profile of Crude Protein Tyrosine Kinase on Plasma Membrane of Merino Sheep Spermatozoa Using the Method of SDS-Page (Sodium Dodecyl Sulphate-Polyacrilamide Gel Electrophoresis)

The research aims to find out profile of crude protein tyrosine kinase on plasma membrane of Merino sheep spermatozoa. The research used fresh semen sample collected using artificial vagina technique, next it was centrifuged to separate spermatozoa from seminal plasma. Then, spermatozoa (pellet) was isolated. After obtaining spermatozoa isolate, analysis on protein tyrosine kinase using  SDS-PAGE method was conducted. The method to analyze protein using SDS-PAGE to separate protein based on molecular weight. Analysis on molecular weight was conducted by comparing  result of isolate tapes with protein marker tapes. Running on  SDS-PAGE gel isolate protein plasma membrane of Merino sheep spermatozoa in the research resulted in 13 protein tapes with average molecular weights of 115.44 kDa; 95.78 kDa; 78.22 kDa; 69.02 kDa; 62.33 kDa; 53.72 kDa; 40.56 kDa; 30.74 kDa; 21.16 kDa; 13.67 kDa; 10.85 kDa; 9.49 and 8.07 kDa kDa. From this result, the second tape with the molecular weight of  95.78 kDa was believed to be protein tyrosine kinase tape however it needs to be confirmed further using Western Blot method.Keywords: tyrosine kinase, membrane of Merino sheep spermatozoa , SDS-PAG

Identifikasi Senyawa Aktif Ekstrak Daun Permot (Passiflora foetida Linn.) dan Potensi sebagai Bioinsektisida Nyamuk Aedes aegypti

Surat Keputusan Rektor Universitas Airlangga tentang Pelaksanaan Kegiatan Penelitian Unggulan Perguruan Tinggi Sumber Biaya Bantuan Operasional Perguruan Tinggi Negeri (BOPTN) Tahun Anggaran 2013 Universitas Airlangga dengan Judul " Identifikasi Senyawa Aktif Ekstrak Daun Permot (Passiflora foetida Linn.) dan Potensi sebagai Bioinsektisida Nyamuk Aedes aegypti

Mapping Penyakit Parasit Saluran Pencernaan pada Sapi Madura di Kabupaten Bangkalan Madura

Surat Keputusan Dekan Fakultas Kedokteran Hewan tentang Penelitian yang Didanai RKAT " Mapping Penyakit Parasit Saluran Pencernaan pada Sapi Madura di Kabupaten Bangkalan Madura

Deteksi Penyakit Parasit Intestinal Zoonosis Sebagai Penyakit Strategis pada Sapi Madura

Surat Keputusan Rektor Tentang Pelaksanaan Penelitian Internal Universitas Airlangga Hibah Riset, Mandat, Penelitian Unggulan Fakultas dan Penelitian Dosen Pemula Tahun 2018 dengan judul " Deteksi Penyakit Parasit Intestinal Zoonosis Sebagai Penyakit Strategis pada Sapi Madura

Tetracycline Resistance Gene In Streptococcus agalactiae Isolated From Bivine Subclinical Mastitis In Surabaya, Indonesia

The aim of this research was to isolate, identify and determine tetO resistance genes in tetracycline-resistant Streptococcus agalactiae isolated from cows with subclinical mastitis in Surabaya and surrounding areas of Indonesia. Milk samples from cows with subclinical mastitis in six dairy farms were collected. S. agalactiae was isolated and antibiotic resistance was determined. Results showed that out of 173 samples analyzed, 131 (75.7%) were positive for California Mastitis Test.S. agalactiae was isolated in 36 out of the 131 CMT-positive samples. Antibiotic sensitivity test revealed that out of 36 S. agalactiae samples, nine were resistant to tetracycline. PCR analysis showed that six of the nine tetracycline resistant S. agalactiae isolates were positive for the tetO resistance genes Key words: Streptococcus agalactiae, subclinical mastitis, tetO gene, tetracycline resistanc

Identification of Active Compounds of Ethanol Extract of Citrus amblycarpa leaves by Analysis of Thin-layer Chromatography and Gas Chromatography-Mass Spectrometry as Bioinsecticide Candidates for Mosquitoes

BACKGROUND: The use of active compounds from plants becomes an alternative to control mosquitoes nowadays and in the future because they are environmentally-friendly and do not cause health problems. Citrus amblycarpa is a local orange of South Kalimantan potential as bioinsecticidal, which commonly used for controlling mosquitoes. Therefore, research needs to be done to find out the benefits of C. amblycarpa leaves as bioinsecticidal. AIM: The research aimed to identify active compounds contained in the extract ethanol of C. amblycarpa leaves as bioinsecticidal against mosquitoes. RESULTS: Based on thin-layer chromatography test, there were some secondary metabolite compounds found such as terpenoids/steroids, flavonoids, polyphenols, and saponins. Gas chromatography-mass spectrometry (GC-MS) test revealed that there were ten primary components of the fraction. The components were Maragenin I (18,82%), 1,3-benzenedicarboxamide (12.28%), 2,3,8-trioxocephalotaxane (10.39%), aristolone, 2H-cyclopropa[a] naphthalene-2-one, noruns-12-ene (7.46%), palmitic acid, n-hexadecanoic acid (7.21%), stigmasterol, demecolcine (7.03%), alpha-tocopherol (5.88%), 2,4,5-trimethylphenol, pseudocumenol (4.21%), germacrene-D (3.45%), and 9-octadecenoic acid (3.36%). CONCLUSION: These active compounds possess biological activity as bioinsecticidal. It was expected that those active compounds in C. amblycarpa leaves could be applied for controlling mosquitoes by replacing the use of resistant temephos