Anti-Hypochlorite, Antioxidant, and Catalytic Activity of Three Polyphenol-Rich Super-Foods Investigated with the Use of Coumarin-Based Sensors

The anti-hypochlorite activity of açaí (Euterpe oleracea Mart.), goji (Lycium barbarum L.) and schisandra (Schisandra chinensis) fruit extracts were assessed by determining the reactive chlorine species (RCS)-scavenging ability of these three “super-food” berries. In addition, the aqueous extracts obtained were employed as both the media and the catalyst in a green chemistry approach to the synthesis of a coumarin-based fluorescence turn-off sensor, which was then used for anti-hypochlorite activity testing. The aqueous extracts were also assessed for total phenolic content (TPC), using the Folin–Ciocalteu method, and the antioxidant activity using the ABTS+• assay. Moreover, the main water-soluble polyphenolic constituents of the extracts were identified by the HPLC-PDA-ESI-MS technique. Among the extracts tested, açaí demonstrated the highest anti-hypochlorite and antioxidant activities, while the highest TPC value was found for the goji extract. All extracts demonstrated modest catalytic activity as Knoevenagel condensation catalysts

ASPEKTY EKONOMICZNE UPRAWY MIESZANKI STRĄCZKOWO-ZBOŻOWEJ W PORÓWNANIU DO CZYSTEGO SIEWU ŁUBINU WĄSKOLISTNEGO I JĘCZMIENIA JAREGO

The aim of the study was to present economic evaluation of blue lupine with spring barley mixtures compared to pure sowing of blue lupine and barley. The source material are the results of a field experiment carried out in 2011-2014 at the Institute of Soil Science and Plant Cultivation – Agricultural Experimental Station Grabów (Mazowieckie Province). The analysis showed that the production value in each year varied, however, the highest level for all crops recorded in 2014. This variation mainly resulted from the sale prices and the yield obtained in the years of the study. Analyzing the structure of production costs, it was found that the largest share of costs accounted for fertilization - especially in cultivation of barley, while the lowest costs were incurred for plant protection. The highest level of gross margin, provided the cultivation of mixtures of blue lupine with a cereal. Comparing the years, it was found that the highest level of gross margin for all crops were obtained in 2014

Effect of Pre-Sowing Magnetic Field Treatment on Enzymes and Phytohormones in Pea (Pisum sativum L.) Seeds and Seedlings

The aim of the presented studies was to evaluate the magnetic field (MF) effect on changes in some enzymes and phytohormones that takes place in the process of seed germination and growth of seedlings. Studies were led in the climatic chambers HERAEUS, on Petri dishes during six consecutive days. Pea seeds were divided into three groups from which one was the control (without stimulation) and two were treated with different doses of magnetic field (30 and 85 mT, respectively). Contents of amylolytic enzymes (AE) and phytohormones were determined at seven terms (0; 24; 48; 72; 96; 120 and 144 h) after placing them on the dishes. A favorable effect of seed stimulation with MF was found on the biochemical processes in the germinating seeds and pea seedlings. The size of changes in enzyme concentration was dependent mainly on the advancement of germination process (i.e., on length of time in which the seeds were on the dishes) and the MF treatment. The use of MF also had an effect on the increase in hormone content in the seeds and organs of seedlings, but values from objects treated with different doses did not differ significantly

Effect of Pre-Sowing Magnetic Field Treatment on Enzymes and Phytohormones in Pea (<i>Pisum sativum</i> L.) Seeds and Seedlings

The aim of the presented studies was to evaluate the magnetic field (MF) effect on changes in some enzymes and phytohormones that takes place in the process of seed germination and growth of seedlings. Studies were led in the climatic chambers HERAEUS, on Petri dishes during six consecutive days. Pea seeds were divided into three groups from which one was the control (without stimulation) and two were treated with different doses of magnetic field (30 and 85 mT, respectively). Contents of amylolytic enzymes (AE) and phytohormones were determined at seven terms (0; 24; 48; 72; 96; 120 and 144 h) after placing them on the dishes. A favorable effect of seed stimulation with MF was found on the biochemical processes in the germinating seeds and pea seedlings. The size of changes in enzyme concentration was dependent mainly on the advancement of germination process (i.e., on length of time in which the seeds were on the dishes) and the MF treatment. The use of MF also had an effect on the increase in hormone content in the seeds and organs of seedlings, but values from objects treated with different doses did not differ significantly

Response of spring wheat to NPK and S fertilization. The content and uptake of macronutrients and the value of ionic ratios

The aim of the study was to evaluate the effect of nitrogen (N) and sulphur (S) fertilizers on the content and uptake of macronutrients by grain dry mass (DM) of spring wheat. A field experiment was conducted in southeastern Poland on Cambisols (WRB 2007), in conditions of low S content in the soil. The experiment included 2 factors: fertilization with N (0, 40, 80, 120 kg ha-1) and with S (0, 50 kg ha-1)

Improvement of the Content and Uptake of Micronutrients in Spring Rye Grain DM Through Nitrogen and Sulfur Supplementation

The aim of this field experiment was to analyze the influence of different nitrogen and sulfur doses on yield as well as the content and uptake of iron (Fe), manganese (Mn), copper (Cu) and zinc (Zn) by spring rye grain. The study was conducted in south-eastern Poland (2009&ndash;2011) on Cambisols (WRB 2015), in conditions of low sulfur content in soil. The experiment included four doses of N fertilization (0, 30, 60 and 90 kg ha&minus;1) and two doses of S supplementation (0 and 40 kg ha&minus;1). The analysis showed that fertilization with N and S had a positive effect on the studied features of spring rye. The highest grain yields were found after use of 90 kg N ha&minus;1. The grain of rye fertilized with these doses of N was characterized by the highest concentration and uptake of tested microelements. The supplementation of sulfur in a dose of 40 kg S ha&minus;1 improved the nitrogen effect, because the rye grain yield and the content and uptake of micronutrients (except Mn) by rye grain dry mass increased. The highest yield of spring rye grain and accumulation of Mn and Zn and intake of Mn, Zn and Cu by grain dry mass (DM) were obtained in the vegetation season of 2011, which was characterized by an optimal rainfall distribution. The highest accumulation of Fe and Cu and intake of Fe were obtained in the vegetation season of 2009. Significant correlations were found also between grain yield and the content and uptake of all studied micronutrients. The supplementation of NPK fertilization with sulfur can be a good means of agronomic biofortification for spring rye in order to increase the content and uptake of Fe, Mn, Zn and Cu

Physical assessment, spectroscopic and chemometric analysis of starch-based foils with selected functional additives.

The paper presents the results of studies related to the impact of functional additives in the form of polylactide (PLA), polyvinyl alcohol (PVA), and keratin hydrolysate (K) on the physical characteristics of biopolymer foils. TPS granulate was obtained using a TS-45 single-screw extruder with L/D = 16. Foil was produced with the use of an L/D = 36 extruder with film-blowing section. The impact of the quantity and type of the functional additives on the processing efficiency and energy consumption of granulate extrusion, as well as the physical characteristics of the foil produced: thickness, basis weight, and colour were determined. By measuring the FTIR spectra it was determined the type and origin of the respective functional groups. It was observed that foils produced from granulates with the addition of 3% PVA were characterised by the lowest thickness and basis weight. Addition of 2 and 3% of PLA increased thickness and basis weight of starch-based foils significantly. Increasing the content of keratin in SG/K samples resulted in a decrease of brightness and intensify the yellow tint of foils, especially when 2 and 3% of keratin was used. In terms of the other samples, it was observed that the colour remained almost unchanged irrespective of the percentage content of the additive used. Infrared analyses conducted on foil containing PVA, PLA, and K revealed a change in spectra intensity in the frequency range associated with-OH groups originating from the forming free, intra- and intermolecular hydrogen bonds. Based on an analysis of the respective bands within the IR range it was also concluded that considerable structural changes took place with respect to the glycosidic bonds of starch itself. The application of the mentioned additives had a significant structural impact on the produced starch-based foils. Furthermore, the conducted UV-Vis analyses revealed a substantial increase in absorbance and a related reduction of the permeability (colour change) of the obtained materials in the range of ultraviolet and visible light

Spectroscopic and theoretical studies of fluorescence effects induced by the ESIPT process in a new derivative 2-Hydroxy-N-(2-phenylethyl)benzamide - Study on the effects of pH and medium polarity changes.

The paper presents the results of studies conducted with the use of stationary and time-resolved fluorescence spectroscopy for the new derivative 2-Hydroxy-N-(2-phenylethyl)benzamide (SAL-3) in aqueous solutions with various concentrations of hydrogen ions as well as in solvent mixtures (i.e. media with changing polarity/polarizability). For the compound selected for the study placed in aqueous solutions with varying concentrations of hydrogen ions, the fluorescence emission spectra revealed a single emission band within most of the pH range, however, at low pH (pH<3) a significant broadening (noticeable effect of dual fluorescence) and shifting of the band was observed. Whereas, for water and polar (protic) solvents, we observed a very interesting phenomenon of dual fluorescence never before reported for this particular group of analogues (with the specific substituent system). Based on the results of the experiments, it was observed that the presented effects may be related both with conformational effects (related to the possible positioning of the-OH group on the side of the carbonyl system, which facilitates the possibility of proton transfer) as well as, most importantly, the effects of excited state intramolecular proton transfer (ESIPT-Excited State Intramolecular Proton Transfer) related in this case with the necessary (new/previously unobserved in published literature) presence of ionic and non-ionic forms of the compound). Both the conducted quantum-mechanical [TD]DFT-Time-Dependent Density Functional Theory) calculations and excited state dipole moment change calculations for the analyzed molecule in solvents with varying pH confirmed the association between the observed fluorescence phenomena and the two aforementioned effects