246 research outputs found
Anterior segment diseases presented in an interactive videotape format
Get PDFWith the overwhelming amount of information provided in ocular disease courses, it is often useful to have a convenient method for supplementing course material. With this in mind, a videotape has been produced which will serve as a convenient and effective teaching aid for second year students. Rather than a simple presentation of facts, this tape is formatted in a manner that encourages participative learning. The student extracts the relevant facts from the case history, forms her/his own diagnosis, observes the recorded eye condition, makes a differential diagnosis and develops a treatment plan. All of this information is available within the tape itself
Secondary Lead Poisoning in Golden Eagle and Ferruginous Hawk Chicks consuming Shot Black-tailed Prairie Dogs, Thunder Basin National Grassland, Wyoming
Get PDFRecreational shooting of black-tailed prairie dogs (Cynomys ludovicianus) is a common activity at Thunder Basin National Grassland (TBNG), Wyoming. The prairie dog carcasses left in the area are scavenged by coyotes (Canis latrans), raptors, and other animals. These scavengers are susceptible to lead (Pb) poisoning if they consume Pb bullet fragments or Pb shot when scavenging the shooter-killed prairie dogs. In 2000, a local rehabilitator noted an increase of Pb poisoning cases in raptors (L.Layton, pers. comm. 3/30/01) from the area. We collected several shooter-killed prairie dog carcasses from TBNG for determining if Pb fragments remained embedded in the tissue that potentially would be consumed by raptors. Radiographs showed fragments consistent with Pb to be present. In 2002, we conducted a more in-depth study to determine if Pb poisoning was occurring in raptors at TBNG by documenting the number of raptors on prairie dogs at colonies where shooting occurred, assaying bullet fragments in shot prairie dogs to determine Pb content, and analyzing blood and feather samples of ferruginous hawk (Buteo regalis) and golden eagle (Aquila chrysaetos) nestlings and feathers from burrowing owls (Athene cunicularia) for clinical signs of Pb poisoning.
We observed raptors foraging at prairie dog colonies and collected data on the number of shooters shooting at prairie dog colonies. To determine if raptors preferred foraging on shot prairie dogs, we compared raptor use at prairie dog colonies where shooting occurred to raptor use at prairie dog colonies where shooting did not occur. Shooter intensity did not predict raptor use. We also collected prairie dog carcasses and examined them for Pb shot fragments. We detected metal fragments in four of ten prairie dog carcasses. The total weight of the fragments found in each carcass ranged from 10 โ 146 mg. Copper was the primary metal detected in 3 of 4 carcasses; but, significant amounts of Pb (20 mg, 28 mg, and 124 mg) were found in the three carcasses. Blood Pb concentrations in ferruginous hawk nestlings were below sub-clinical levels at TBNG and the control site near Rawlins, Wyoming. Analysis of red blood cell delta-aminolevulinic acid dehydratase activity, hemoglobin levels, and protoporphyrin levels also did not indicate Pb poisoning in ferruginous hawk nestlings. Additionally, blood and feather samples from golden eagle nestlings and feather samples from burrowing owls (juveniles and adults) at TBNG did not indicate Pb poisoning. Although ferruginous hawks and golden eagles (and possibly burrowing owls) scavenge on the carcasses of shot prairie dogs and some carcasses contained Pb-bullet fragments, we did not detect Pb poisoning in any of the birds. Lead poisoning may become important if the availability of alternate food sources decreases or shooter intensity increases
Building Interprofessional Global Health Infrastructure at a University and Health System: Navigating Challenges and Scaling Successes
Get PDFMission:
Global Jefferson will create sustainable programs of global distinction through collaboration that position Jefferson as a local and international destination and resource for education, research, and clinical activities.
Global Jefferson is supported by the Associate Provost for Global Affairs, part of the Office of the Provost.
Global activity at Jefferson includes: Global Health Initiatives Committee (GHIC) Service Learning Global Research & Exchange between institutions Pre-clinical, translational, clinical, and applied research
Poster presented at: 8th Annual Global Health Conference of the Consortium of Universities for Global Health (CUGH)https://jdc.jefferson.edu/globalhealthposters/1000/thumbnail.jp
A comparison of collision cross section values obtained via travelling wave ion mobility-mass spectrometry and ultra high performance liquid chromatography-ion mobility-mass spectrometry : application to the characterisation of metabolites in rat urine
Get PDFA comprehensive Collision Cross Section (CCS) library was obtained via Travelling Wave Ion Guide mobility measurements through direct infusion (DI). The library consists of CCS and Mass Spectral (MS) data in negative and positive ElectroSpray Ionisation (ESI) mode for 463 and 479 endogenous metabolites, respectively. For both ionisation modes combined, TWCCSN2 data were obtained for 542 non-redundant metabolites. These data were acquired on two different ion mobility enabled orthogonal acceleration QToF MS systems in two different laboratories, with the majority of the resulting TWCCSN2 values (from detected compounds) found to be within 1% of one another. Validation of these results against two independent, external TWCCSN2 data sources and predicted TWCCSN2 values indicated to be within 1-2% of these other values. The same metabolites were then analysed using a rapid reversed-phase ultra (high) performance liquid chromatographic (U(H)PLC) separation combined with IM and MS (IM-MS) thus providing retention time (tr), m/z and TWCCSN2 values (with the latter compared with the DI-IM-MS data). Analytes for which TWCCSN2 values were obtained by U(H)PLC-IM-MS showed good agreement with the results obtained from DI-IM-MS. The repeatability of the TWCCSN2 values obtained for these metabolites on the different ion mobility QToF systems, using either DI or LC, encouraged the further evaluation of the U(H)PLC-IM-MS approach via the analysis of samples of rat urine, from control and methotrexate-treated animals, in order to assess the potential of the approach for metabolite identification and profiling in metabolic phenotyping studies. Based on the database derived from the standards 63 metabolites were identified in rat urine, using positive ESI, based on the combination of tr, TWCCSN2 and MS data.</p
Structural basis for high-affinity binding of LEDGF PWWP to mononucleosomes
Get PDFLens epithelium-derived growth factor (LEDGF/p75)
tethers lentiviral preintegration complexes (PICs) to
chromatin and is essential for effective HIV-1 replication.
LEDGF/p75 interactions with lentiviral
integrases are well characterized, but the structural
basis for how LEDGF/p75 engages chromatin is
unknown. We demonstrate that cellular LEDGF/p75
is tightly bound to mononucleosomes (MNs). Our
proteomic experiments indicate that this interaction
is direct and not mediated by other cellular factors.
We determined the solution structure of LEDGF
PWWP and monitored binding to the histone H3
tail containing trimethylated Lys36 (H3K36me3) and
DNA by NMR. Results reveal two distinct functional
interfaces of LEDGF PWWP: a well-defined hydrophobic
cavity, which selectively interacts with the
H3K36me3 peptide and adjacent basic surface,
which non-specifically binds DNA. LEDGF PWWP
exhibits nanomolar binding affinity to purified
native MNs, but displays markedly lower affinities
for the isolated H3K36me3 peptide and DNA.
Furthermore, we show that LEDGF PWWP preferentially
and tightly binds to in vitro reconstituted
MNs containing a tri-methyl-lysine analogue at
position 36 of H3 and not to their unmodified
counterparts. We conclude that cooperative
binding of the hydrophobic cavity and basic
surface to the cognate histone peptide and DNA
wrapped in MNs is essential for high-affinity
binding to chromatin
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