1,706 research outputs found

    Low-effort place recognition with WiFi fingerprints using deep learning

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    Using WiFi signals for indoor localization is the main localization modality of the existing personal indoor localization systems operating on mobile devices. WiFi fingerprinting is also used for mobile robots, as WiFi signals are usually available indoors and can provide rough initial position estimate or can be used together with other positioning systems. Currently, the best solutions rely on filtering, manual data analysis, and time-consuming parameter tuning to achieve reliable and accurate localization. In this work, we propose to use deep neural networks to significantly lower the work-force burden of the localization system design, while still achieving satisfactory results. Assuming the state-of-the-art hierarchical approach, we employ the DNN system for building/floor classification. We show that stacked autoencoders allow to efficiently reduce the feature space in order to achieve robust and precise classification. The proposed architecture is verified on the publicly available UJIIndoorLoc dataset and the results are compared with other solutions

    Cyclic di-GMP mediates a histidine kinase/phosphatase switch by noncovalent domain cross-linking

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    Histidine kinases are key components of regulatory networks in bacteria. Although many of these enzymes are bifunctional, mediating both phosphorylation and dephosphorylation of downstream targets, the molecular details of this central regulatory switch are unclear. We showed recently that the universal second messenger cyclic di-guanosine monophosphate (c-di-GMP) drives Caulobacter crescentus cell cycle progression by forcing the cell cycle kinase CckA from its default kinase into phosphatase mode. We use a combination of structure determination, modeling, and functional analysis to demonstrate that c-di-GMP reciprocally regulates the two antagonistic CckA activities through noncovalent cross-linking of the catalytic domain with the dimerization histidine phosphotransfer (DHp) domain. We demonstrate that both c-di-GMP and ADP (adenosine diphosphate) promote phosphatase activity and propose that c-di-GMP stabilizes the ADP-bound quaternary structure, which allows the receiver domain to access the dimeric DHp stem for dephosphorylation. In silico analyses predict that c-di-GMP control is widespread among bacterial histidine kinases, arguing that it can replace or modulate canonical transmembrane signaling

    Transduction of an immortalized olfactory ensheathing glia cell line with the green fluorescent protein (GFP) gene: evaluation of its neuroregenerative capacity as a proof of concept

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    Olfactory ensheathing glia (OEG) cells are known to foster axonal regeneration of central nervous system (CNS) neurons. Several lines of reversibly immortalized human OEG (ihOEG) have been previously established that enabled to develop models for their validation in vitro and in vivo. In this work, a constitutively GFP-expressing ihOEG cell line was obtained, and named Ts14-GFP. Ts14-GFP neuroregenerative ability was similar to that found for the parental line Ts14 and it can be assayed using in vivo transplantation experimental paradigms, after spinal cord or optic nerve damage. Additionally, we have engineered a low-regenerative ihOEG line, hTL2, using lentiviral transduction of the large T antigen from SV40 virus, denominated from now on Ts12. Ts12 can be used as a low regeneration control in these experiments.Comment: 22 pages, 3 Figure

    Excitability in a nonlinear magnetoacoustic resonator

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    We report a nonlinear acoustic system displaying excitability. The considered system is a magnetostrictive material where acoustic waves are parametrically generated. For a set of parameters, the system presents homoclinic and heteroclinic dynamics, whose boundaries define a excitability domain. The excitable behaviour is characterized by analyzing the response of the system to different external stimuli. Single spiking and bursting regimes have been identified.Comment: 4 pages, 5 figure

    Transduction of an immortalized olfactory ensheathing glia cell line with the green fluorescent protein (GFP) gene: Evaluation of its neuroregenerative capacity as a proof of concept.

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    Olfactory ensheathing glia (OEG) cells are known to foster axonal regeneration of central nervous system (CNS) neurons. Several lines of reversibly immortalized human OEG (ihOEG) have been previously established that enabled to develop models for their validation in vitro and in vivo. In this work, a constitutively GFP-expressing ihOEG cell line was obtained, and named Ts14-GFP. Ts14-GFP neuroregenerative ability was similar to that found for the parental line Ts14 and it can be assayed using in vivo transplantation experimental paradigms, after spinal cord or optic nerve damage. Additionally, we have engineered a low-regenerative ihOEG line, hTL2, using lentiviral transduction of the large T antigen from SV40 virus, denominated from now on Ts12. Ts12 can be used as a low regeneration control in these experiments.pre-print281 K

    Nematodos bacteriófagos como bioindicadores y como organismos asociados a los procesos de biorremediacion

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    De la gran diversidad de organismos que viven en el suelo, los nematodos bacteriófagos constituyen un sujeto de estudio interesante en la búsqueda de bioindicadores adecuados para reflejar el grado de perturbación del suelo y la marcha de los procesos de recuperación. En el presente trabajo se hace una evaluación de distintas situaciones relacionadas con suelos alterados por actividades antrópicas y su relación con las poblaciones de nematodos bacteriófagos y bacterias, analizadas en laboratorio y a campo. Los resultados obtenidos muestran que el comportamiento de estos organismos responde a la evolución del los niveles de concentración del contaminante.Fil: Otero, María del C.. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Torres, N.. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Plaza, Gloria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones en Energía no Convencional. Universidad Nacional de Salta. Facultad de Ciencias Exactas. Departamento de Física. Instituto de Investigaciones en Energía no Convencional; ArgentinaFil: Pérez Brandan, C.. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentin

    Explicit Evidence Systems with Common Knowledge

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    Justification logics are epistemic logics that explicitly include justifications for the agents' knowledge. We develop a multi-agent justification logic with evidence terms for individual agents as well as for common knowledge. We define a Kripke-style semantics that is similar to Fitting's semantics for the Logic of Proofs LP. We show the soundness, completeness, and finite model property of our multi-agent justification logic with respect to this Kripke-style semantics. We demonstrate that our logic is a conservative extension of Yavorskaya's minimal bimodal explicit evidence logic, which is a two-agent version of LP. We discuss the relationship of our logic to the multi-agent modal logic S4 with common knowledge. Finally, we give a brief analysis of the coordinated attack problem in the newly developed language of our logic

    Spectral stability of noncharacteristic isentropic Navier-Stokes boundary layers

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    Building on work of Barker, Humpherys, Lafitte, Rudd, and Zumbrun in the shock wave case, we study stability of compressive, or "shock-like", boundary layers of the isentropic compressible Navier-Stokes equations with gamma-law pressure by a combination of asymptotic ODE estimates and numerical Evans function computations. Our results indicate stability for gamma in the interval [1, 3] for all compressive boundary-layers, independent of amplitude, save for inflow layers in the characteristic limit (not treated). Expansive inflow boundary-layers have been shown to be stable for all amplitudes by Matsumura and Nishihara using energy estimates. Besides the parameter of amplitude appearing in the shock case, the boundary-layer case features an additional parameter measuring displacement of the background profile, which greatly complicates the resulting case structure. Moreover, inflow boundary layers turn out to have quite delicate stability in both large-displacement and large-amplitude limits, necessitating the additional use of a mod-two stability index studied earlier by Serre and Zumbrun in order to decide stability

    GPS, LiDAR and VNIR data to monitor the spatial behavior of grazing sheep

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    Traditional knowledge about the behavior of grazing livestock is about to disappear. Shepherds well know that sheep behavior follows non-random patterns. As a novel alternative to seeking behavioral patterns, this study quantified the grazing activities of two sheep flocks of Churra breed (both in the same area but separated by 10 years) based on Global Position System (GPS) monitoring and remote monitoring sensing techniques. In the first monitoring period (2009-10), geolocations were recorded every 5 min (4, 240 records), while in the second one (2018-20), records were taken every 30 min (7, 636 records). The data were clustered based on the day/night and the activity (resting, moving, or grazing). An airborne LiDAR dataset was used to study the slope, aspect, and vegetation height. Four visible-infrared orthophotographs were mosaicked and classified to obtain the land use/land cover (LU/LC) map. Then, GPS locations were overlain on the terrain features, and a Chi-square test evaluated the relationships between locations and terrain features. Three spatial statistics (directional distribution, Kernel density, and Hot Spot analysis) were also calculated. Results in both monitoring periods suggested that the spatial distribution of free-grazing ewes was non-random. The flocks showed strong preferences for grazing areas with gentle north-facing slopes, where the herbaceous layer formed by pasture predominates. The geostatistical analyses of the sheep locations corroborated those preferences. Geotechnologies have emerged as a potent tool to demonstrate the influence of environmental and terrain attributes on the non-random spatial behavior of grazing sheep. © 2022 Malque Publishing. All rights reserved
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