TEMPus VoLA: The timed Epstein multi-pressure vessel at low accelerations.

The field of planetary system formation relies extensively on our understanding of the aerodynamic interaction between gas and dust in protoplanetary disks. Of particular importance are the mechanisms triggering fluid instabilities and clumping of dust particles into aggregates, and their subsequent inclusion into planetesimals. We introduce the timed Epstein multi-pressure vessel at low accelerations, which is an experimental apparatus for the study of particle dynamics and rarefied gas under micro-gravity conditions. This facility contains three experiments dedicated to studying aerodynamic processes: (i) the development of pressure gradients due to collective particle-gas interaction, (ii) the drag coefficients of dust aggregates with variable particle-gas velocity, and (iii) the effect of dust on the profile of a shear flow and resultant onset of turbulence. The approach is innovative with respect to previous experiments because we access an untouched parameter space in terms of dust particle packing fraction, and Knudsen, Stokes, and Reynolds numbers. The mechanisms investigated are also relevant for our understanding of the emission of dust from active surfaces, such as cometary nuclei, and new experimental data will help interpreting previous datasets (Rosetta) and prepare future spacecraft observations (Comet Interceptor). We report on the performance of the experiments, which has been tested over the course of multiple flight campaigns. The project is now ready to benefit from additional flight campaigns, to cover a wide parameter space. The outcome will be a comprehensive framework to test models and numerical recipes for studying collective dust particle aerodynamics under space-like conditions

The Nitrate Transporter MtNPF6.8 (MtNRT1.3) Transports Abscisic Acid and Mediates Nitrate Regulation of Primary Root Growth in Medicago truncatula

Elongation of the primary root during postgermination of Medicago truncatula seedlings is a multigenic trait that is responsive to exogenous nitrate. A quantitative genetic approach suggested the involvement of the nitrate transporter MtNPF6.8 (for Medicago truncatula NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER Family6.8) in the inhibition of primary root elongation by high exogenous nitrate. In this study, the inhibitory effect of nitrate on primary root elongation, via inhibition of elongation of root cortical cells, was abolished in npf6.8 knockdown lines. Accordingly, we propose that MtNPF6.8 mediates nitrate inhibitory effects on primary root growth in M. truncatula. pMtNPF6.8:GUS promoter-reporter gene fusion in Agrobacterium rhizogenes-generated transgenic roots showed the expression of MtNPF6.8 in the pericycle region of primary roots and lateral roots, and in lateral root primordia and tips. MtNPF6.8 expression was insensitive to auxin and was stimulated by abscisic acid (ABA), which restored the inhibitory effect of nitrate in npf6.8 knockdown lines. It is then proposed that ABA acts downstream of MtNPF6.8 in this nitrate signaling pathway. Furthermore, MtNPF6.8 was shown to transport ABA in Xenopus spp. oocytes, suggesting an additional role of MtNPF6.8 in ABA root-to-shoot translocation. (15)NO3 (-)-influx experiments showed that only the inducible component of the low-affinity transport system was affected in npf6.8 knockdown lines. This indicates that MtNPF6.8 is a major contributor to the inducible component of the low-affinity transport system. The short-term induction by nitrate of the expression of Nitrate Reductase1 (NR1) and NR2 (genes that encode two nitrate reductase isoforms) was greatly reduced in the npf6.8 knockdown lines, supporting a role of MtNPF6.8 in the primary nitrate response in M. truncatula

S-nitrosothiols regulate nitric oxide production and storage in plants through the nitrogen assimilation pathway

Nitrogen assimilation plays a vital role in plant metabolism. Assimilation of nitrate, the primary source of nitrogen in soil, is linked to the generation of the redox signal nitric oxide (NO). An important mechanism by which NO regulates plant development and stress responses is through S-nitrosylation, that is, covalent attachment of NO to cysteine residues to form S-nitrosothiols (SNO). Despite the importance of nitrogen assimilation and NO signalling, it remains largely unknown how these pathways are interconnected. Here we show that SNO signalling suppresses both nitrate uptake and reduction by transporters and reductases, respectively, to fine tune nitrate homeostasis. Moreover, NO derived from nitrate assimilation suppresses the redox enzyme S-nitrosoglutathione Reductase 1 (GSNOR1) by S-nitrosylation, preventing scavenging of S-nitrosoglutathione, a major cellular bio-reservoir of NO. Hence, our data demonstrates that (S)NO controls its own generation and scavenging by modulating nitrate assimilation and GSNOR1 activity.5540

Optimal strategies for hedging portfolios of unit-linked life insurance contracts with minimum death guarantee

In this paper, we are interested in hedging strategies which allow the insurer to reduce the risk to their portfolio of unit-linked life insurance contracts with minimum death guarantee. Hedging strategies are developed in the Black and Scholes model and in the Merton jump-diffusion model. According to the new frameworks (IFRS, Solvency II and MCEV), risk premium is integrated into our valuations. We will study the optimality of hedging strategies by comparing risk indicators (Expected loss, volatility, VaR and CTE) in relation to transaction costs and costs generated by the re-hedging error. We will analyze the robustness of hedging strategies by stress-testing the effect of a sharp rise in future mortality rates and a severe depreciation in the price of the underlying asset.Unit-linked Death guarantee Hedging strategies Transaction and error of re-hedging costs Risk indicators Stress-testing Unites de comptes Garanties deces Strategies de couverture Couts de transaction et erreur de couverture Indicateurs de risque Stress-testing

Síndrome antifosfolípido primario en escolar femenino con hipertensión arterial

El síndrome antifosfolípido (SAF) es una enfermedad autoinmune caracterizada por episodios trombóticos recurrentes y la presencia de anticuerpos antifosfolípidos positivos en sangre. Se encuentra asociada con otras enfermedades como el Lupus eritematoso sistémico siendo una entidad muy rara en el 2,8 % de los niños con trombosis. El presente caso trata de escolar femenina de 8 años con enfermedad cerebral vascular e hipertensión arterial (HTA), realizándose estudios de extensión entre ellos pruebas inmunológicas, siendo positivas para anticuerpos antifosfolípidos; las pruebas de función renal evidencian proteinuria, alteración de la función renal siendo signo indirecto de daño glomerular. En los pacientes con SAF y daño renal el 93 % cursan con HTA secundaria a arteriosclerosis, hiperplasia de la íntima, fibrosis arteriolar y microangiopatía trombótica por depósito de anticuerpos antifosfolípidos. Las enfermedades inmunológicas son causa de HTA, es importante sospecharlas durante el estudio a fin de evitar lesiones irreversibles a largo plazo. TitlePrimary antiphospholipid syndrome in female school with arterial hypertensionAbstract Antiphospholipid syndrome (APS) is an autoimmune disease characterized by recurrent thrombotic events and the presence of positive antiphospholipid antibodies in the blood. It is associated with other diseases such as systemic lupus erythematosus, being a very rare entity compromising 2.8 % of children with thrombosis. The present case deals with 8-year-old schoolchildren with vascular cerebral disease and arterial hypertension (HT), with extension studies including immunological tests, being positive for antiphospholipid antibodies; Renal function tests show proteinuria, impaired renal function being an indirect sign of glomerular damage. In patients with APS and renal damage, 93 % had AHT secondary to arteriosclerosis, intimal hyperplasia, arteriolar fibrosis, and thrombotic microangiopathy due to deposition of antiphospholipid antibodies. Immunological diseases are the cause of hypertension and it is important to suspect them during the study in order to avoid irreversible long-term damage. &nbsp

Gender does not influence outcomes and complications in medial unicompartmental knee arthroplasty.

The impact of gender on the outcomes of unicompartmental knee arthroplasty (UKA) remains a topic of active discussion with limited exploration thus far. The study aims to elucidate the gender effect on clinical outcomes, complications, pre- and postoperative radiological outcomes following the implantation of a medial UKA at mid-term follow-up in a large section of patients. This was a single-centre, retrospective cohort study encompassing patients undergoing medial UKA between 2011 and 2019. The International Knee Society (IKS) Knee and Function score, patient satisfaction, complications, revisions, pre- and postoperative radiological outcomes (coronal plane alignment, femoral and tibial component positioning, posterior tibial slope) were evaluated. Survival rate at the time of the last follow-up was also recorded. Of the 366 knees that met the inclusion criteria, 10 were lost to follow-up, accounting for a 2.7% loss. Mean follow-up was 5.2 ± 2 years [2.1-11.3]. Out of the total population, 205 patients were females (57.6%, 205/356) and 151 were males (42.4%, 151/356). Men exhibited superior pre- and postoperative IKS function scores (p = 0.017). However, no significant differences were observed between women and men regarding improvements of IKS Knee and Function scores, radiographic outcomes and implant survivorship. At a mean follow-up of 5 years, this study revealed no significant impact of gender on clinical outcomes and complications in patients undergoing medial UKA. Furthermore, no significant differences were evident in radiographic outcomes, implant positioning and knee phenotype. Level III