Progress with the LOFAR Imaging Pipeline

One of the science drivers of the new Low Frequency Array (LOFAR) is large-area surveys of the low-frequency radio sky. Realizing this goal requires automated processing of the interferometric data, such that fully calibrated images are produced by the system during survey operations. The LOFAR Imaging Pipeline is the tool intended for this purpose, and is now undergoing significant commissioning work. The pipeline is now functional as an automated processing chain. Here we present several recent LOFAR images that have been produced during the still ongoing commissioning period. These early LOFAR images are representative of some of the science goals of the commissioning team members.Comment: 11 pages, 6 figures. Accepted for publication in proceedings of "ISKAF2010 Science Meeting", PoS(ISKAF2010)05

Statistical methods for the analysis of rotation measure grids in large scale structures in the SKA era

To better understand the origin and properties of cosmological magnetic fields, a detailed knowledge of magnetic fields in the large-scale structure of the Universe (galaxy clusters, filaments) is crucial. We propose a new statistical approach to study magnetic fields on large scales with the rotation measure grid data that will be obtained with the new generation of radio interferometers.Comment: 9 pages; to appear as part of 'Cosmic Magnetism' in Proceedings 'Advancing Astrophysics with the SKA (AASKA14)', PoS(AASKA14)11

Interrupting the nitrosative stress fuels tumor-specific cytotoxic T lymphocytes in pancreatic cancer

BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest tumors owing to its robust desmoplasia, low immunogenicity, and recruitment of cancer-conditioned, immunoregulatory myeloid cells. These features strongly limit the success of immunotherapy as a single agent, thereby suggesting the need for the development of a multitargeted approach. The goal is to foster T lymphocyte infiltration within the tumor landscape and neutralize cancer-triggered immune suppression, to enhance the therapeutic effectiveness of immune-based treatments, such as anticancer adoptive cell therapy (ACT). METHODS: We examined the contribution of immunosuppressive myeloid cells expressing arginase 1 and nitric oxide synthase 2 in building up a reactive nitrogen species (RNS)-dependent chemical barrier and shaping the PDAC immune landscape. We examined the impact of pharmacological RNS interference on overcoming the recruitment and immunosuppressive activity of tumor-expanded myeloid cells, which render pancreatic cancers resistant to immunotherapy. RESULTS: PDAC progression is marked by a stepwise infiltration of myeloid cells, which enforces a highly immunosuppressive microenvironment through the uncontrolled metabolism of L-arginine by arginase 1 and inducible nitric oxide synthase activity, resulting in the production of large amounts of reactive oxygen and nitrogen species. The extensive accumulation of myeloid suppressing cells and nitrated tyrosines (nitrotyrosine, N-Ty) establishes an RNS-dependent chemical barrier that impairs tumor infiltration by T lymphocytes and restricts the efficacy of adoptive immunotherapy. A pharmacological treatment with AT38 ([3-(aminocarbonyl)furoxan-4-yl]methyl salicylate) reprograms the tumor microenvironment from protumoral to antitumoral, which supports T lymphocyte entrance within the tumor core and aids the efficacy of ACT with telomerase-specific cytotoxic T lymphocytes. CONCLUSIONS: Tumor microenvironment reprogramming by ablating aberrant RNS production bypasses the current limits of immunotherapy in PDAC by overcoming immune resistance

Tomography of galaxy clusters through low-frequency radio polarimetry

De mens heeft altijd willen weten wat zijn oorsprong is en hoe deze is gerelateerd aan het heelal. Hij heeft dit op verschillende manieren geprobeerd: is er een religieuze achtergrond of een wetenschappelijke? Vaak heeft de mens zijn vragen en antwoorden in de literatuur en beeldende kunst uitgedrukt door middel van gedichten en verhalen, het maken van tekeningen, schilderijen en sculpturen van geweldige omvang en schoonheid. De wetenschappelijke aanpak is natuurlijk een van de beste benaderingen om goede en directe antwoorden te vinden. Dit is de reden waarom ik ongeveer negen jaar geleden de wetenschap ben gaan bestuderen met als resultaat de onderwerpen die in dit proefschrift beschreven zijn. Deze samenvatting heeft als doel in het kort de resultaten weer te geven van mijn werk van de afgelopen vier jaar, waarbij ik de onderwerpen van dit proefschrift in een voor iedereen begrijpelijk en makkelijk leesbaar geheel probeer samen te vatten. Om de details van mijn werk te begrijpen, is het zeer nuttig eerst een globaal idee te geven van hoe het universum waarin we leven is georganiseerd. ... Zie: Samenvatting