Hygiene Sanitation and Detection Of Rhodamin B in Sauce and Formalin in Meatball at Meatball Tavern, Kupang, East Nusa Tenggara

Background: Food safety and sanitation is an essential part of the food industry. While it is important to be able to deliver food quickly and profitably, the importance of food safety and sanitation cannot be underestimated. Fast food retailers such as meatball tavern are common in Kupang, East Nusa Tenggara. Therefore it is worth studying the food safety and sanitation among the existing fast food retailers. This study aimed to examine hygiene sanitation and detection of rhodamin B in sauce and formalin in meatball at meatball tavern, Kupang, East Nusa Tenggara. Subjects and Method: This was a cross-sectional study conducted at meatball taverns, Kupang, East Nusa Tenggara, Indonesia. A total of 16 meatball taverns were selected for this study by total sampling. The dependent variables were hygiene sanitation, rhodamin B content in sauce, and formalin content in meatball. The independent variables were selection of foodstuff, food storage, processing place, equipment, food handlers, food transport, and food provision. The data were collected by observation and laboratory examination. The data were described accordingly. Results: Hygiene and sanitation in food selection and food storage were good (70-100%). Hygiene and sanitation in food handling and processing were fair (50-78%). Hygiene and sanitation of processing places were variable from poor to moderate (36-72%). Hygiene and sanitation of equipment were good (70-100%). Food stewardship was very good (87-100%). Rhodamin B content was absent in sauce. Formalin content was absent in meatball. Conclusion: Hygiene and sanitation levels in various aspect of food preparation and handling at meatball taverns were variable ranging from poor to very good level. Keywords: hygiene, sanitation, meatball, tavern, formalin, rhodamin B

Bayesian estimation of diagnostic accuracy of fecal culture and PCR-based tests for the detection of Salmonella enterica in California cull dairy cattle.

Epidemiological studies of low prevalence disease problems are often hindered by the high cost of diagnostic testing. The objective of this study was to evaluate PCR screening of both individual and pooled fecal samples from culled dairy cows for the invA gene of Salmonella followed by culture to determine if the sensitivity and specificity were comparable to the results from traditional culture methods applied to individual samples. Cows from six different dairies were sampled in all four seasons. A total of 240 individual cow fecal samples, 24 fecal pools and 24 pools of 24-hour tetrathionate enrichment broth were tested. Diagnostic sensitivity of PCR screening followed by culture of PCR positive or indeterminate samples (i.e PCR-CUL method) was lower than that of culture (CUL) when applied to individual fecal samples (94.8%, 99.5%), however the specificity was comparable (99.6% and 97.7% respectively). For pools of five fecal samples and pools of five, 24 h tetrathionate broth samples, the specificity of both tests were comparable (∼98%); however, their sensitivity was only comparable in pooled fecal samples (∼93%) but greater for culture compared to PCR-CUL in pooled broth samples (∼99% versus ∼93%). Compared to culture results from testing of individual fecal samples, testing pooled fecal samples by culture had a relative sensitivity of 74% and relative specificity of 96%, testing pooled fecal samples by PCR-CUL resulted in relative sensitivity of 90% and relative specificity of 96%. Testing of pooled 24-hour enrichment broth by PCR-CUL increased the relative sensitivity and specificity to 100%. PCR testing followed by culture of positive or indeterminate samples is a time saving alternative to traditional methods. In addition, pooling of samples may be a useful method for decreasing cost if study aims can accommodate a moderate loss of relative sensitivity

Knowledge, Preventive Practice, and The Risk of Dengue Hemorrhagic Fever in Kupang, East Nusa Tenggara

Background: Dengue/dengue hemorrhagic fever (DHF) has been one of the most important resurgent tropical diseases in the past 17 years. Its incidence in East Nusa Tenggara has been increasing from 2011 to 2013. This study aimed to investigate the associations between knowledge, preventive practice, and the risk of dengue hemorrhagic fever in Kupang, East Nusa Tenggara. Subjects and Method: This was a case-control study conducted at 8 Puskesmas (Community Health Center) in Kupang, East Nusa Tenggara, Indonesia, from March to May 2016. A total of 72 subjects were selected for this study by fixed disease sampling, consisting of 36 DHF cases and 36 controls. The dependent variable was DHF. The independent variables were knowledge on DHF and DHF preventive practice including draining, hoarding and burying. The data were collected by questionnaire and interview. The data were analyzed by multiple logistic regression. Results: Poor knowledge on DHF (OR= 4.85; 95% CI= 1.62 to 14.49; p= 0.005) and poor DHF preventive practice (OR= 4.72; 95% CI= 1.60 to 13.91; p= 0.005) were associated with an increased risk of DHF. Conclusion: Poor knowledge of DHF and poor DHF preventive practice are associated with an increased risk of DHF. Keywords: knowledge, preventive practice, dengue hemorrhagic fever

Factors Associated with The Use of Antenatal Care in East Sumba, East Nusa Tenggara

Background: Antenatal care (ANC) is very important to pregnant women as it helps prevent mother and child mortality, prevent complications, helps foster a good relationship between the husband and wife, mother and child and father. Good antenatal care includes regular screening which can detect and prevent early complications such as hypertension and pregnancy diabetes; both of which can dramatically affect the fetus. Early detection means regular monitoring and treatment. This study aimed to analyze factors associated with the use of ANC in East Sumba, East Nusa Tenggara. Subjects and Method: This was an cross sectional study conducted in East Sumba, East Nusa Tenggara. A sample of 254 pregnant women were selected for this study by simple random sampling. The dependent variable was use of ANC. The independent variables were being a housewife, receiving husband's support, and social support. The data were collected by questionnaire and analyzed by a multiple logistic regression. Results: The use of ANC was associated with being a housewife (b= 0.88; 95% CI= 1.30 to 4.48; p= 0.005), receiving husband's support (b= 0.72; 95% CI= 0.25 to 0.95; p= 0.035), and social support (b= 1.52; 95% CI= 0.10 to 0.51; p<0.001). Conclusion: The use of ANC is associated with being a housewife, receiving husband's support and social support. Keywords: housewife, husband's support, social support, antenatal care, utilizatio

Service Quality and Passenger Satisfaction in Air Transportation in a Developing Economy: Evidence from Nigeria

This study investigates the quality of service provided for air travel in Nigeria and the level of satisfaction of passengers with the service encountered. It does so by focusing on both the airports and the airlines as service providers. In that way, the study captures much of the effects of the organizational culture, employee behavior, and general norms which influence passenger attitudes. The SERVQUAL model was used in measuring the five dimensions of service. Domestic travel only was studied, with data obtained from well-structured questionnaires to evaluate perception–expectation gaps in airports as well as in airlines. The overall gap score of the Nigerian airports shows an average airport quality of service with a value of 21.8179. This reveals that the perceived service quality is less than passengers’ expectations in Nigerian airport service operations. In the same vein, the gap score of the Nigerian airlines shows an average airline quality-of-service gap with a value of 21.32, which is generally low. This means that the passengers or customers of the airlines during the response period were expecting more service quality from the airlines in the country. The level of service at Nigerian airports needs to be improved so as to encourage passengers to use the airports and as well reduce many queues at baggage collection areas. The air travel industry is currently challenged in a recessed economy resulting in lower service standards, lower patronage, increased missed trips, flight cancellations, passenger complaints, and faltering loyalty. The findings of the study will, therefore, be useful to the managers of airports and airlines, the economic regulator of the aviation industry, and other stakeholders

DETERMINATION OF TOTAL TANIN CONTENTS OF Terminalia Catappa, L. LEAF EXTRACT AND TEST OF ITS ABILITY AS A COMPLEXION AGENT OF Fe (III)

A study of the essential oil content in basil (Ocimum sanctum) and red lemongrass (Cymbopogon nardus L. Rendle) originally grown in Flores island has been conducted. The purpose of this study was to identify the chemotype of the essential oils obtained over stahl distillation using GC-MS. The Ocimum mainly consisted of 55% eugenol following by minor trans-alfa-bergometena, patchulana, and linalool. Meanwhile, the chemotype of the red lemongrass (Cymbopogon nardus L. rendle) were citronellol (22%), citronella, cyclohexamethanol, and viridiflorol

Bacteriological assessment of some borehole water samples in Mile 50, Abakaliki, Ebonyi State, Nigeria

Water as excellent natural resource is meant to be of high quality to reduce the outbreak of water-borne diseases. Bacteriological load of some borehole water samples in Mile 50 Abakaliki were carried out to determine their potability. Fifteen borehole water samples were sampled during rainy and dry season from June to July and November to December 2018 respectively. The total bacterial count was determined by tenfold serial dilution method using peptone water. Eight bacterial species namely Vibrio cholerae, Pseudomonas aeroginosa, Escherichia coli, Staphylococcus aureus, Salmonella typhi, Shigella flexineri, Proteus vulgaris and Klebsiella&nbsp; pneumoniaewere isolated using standard analytical procedures. The bacterium that had the highest frequency of occurrence during both rainy and dry season’s was K. pneumoniae with percentage frequency of&nbsp; 21.81% and 20.79%&nbsp; respectively, and P. vulgaris had the least value of 6.96% during rainy season. E. coli and S. aureus have the least value of 5.94% during dry season. Amoxicillin (30ug) was mostly resisted by the bacterial isolates why being was more susceptible to Ciprofloxacin (10ug) among the antibiotics used for susceptibility test. Two way analysis of variance (ANOVA) was used to determine the level of significance among the bacteriological analyses of both seasons. Therefore, there is need to create awareness about the present situation of the borehole waters and the necessity for further treatment of water by consumers, before it can be used for both drinking and domestic purposes to prevent disease outbreak in the area

As Assessment of Airline Service Quality in a Category One Nation: Focus on Mallam Aminu Kano International Airport

To evaluate airline service quality from passengers’ in a Category One (CAT1) nation. The researchers used quantitative method to evaluate airline quality of service, from passengers’ perspective at Mallam Aminu Kano International Airport (KAN). In a week period, two hundred (200) questionnaires were administered; though one hundred thirty (130) responses were gathered from passengers who travelled with the Arik Air, Aero Contractor, and Azman airlines, these air airlines were chosen because they are preferred passenger\u27s choice. The study findings revealed that independent variables influenced airline\u27s quality of service at the KAN. The outcome of service quality analysis demonstrated that there is a statistical significant relationship between passengers’ perspectives and reliability of the airline services. There is a statistical significant relationship between passengers’ perspectives and affordability of services. Finally, there is a statistical significant link between passenger’s perspectives and comfortability of the airline services. Recommendations: on how airlines and government can create synergy to improve service quality for better and sustain passenger experience, while leveraging on the nation CAT 1 status

Bradykinin improves postischaemic recovery in the rat heart: role of high energy phosphates, nitric oxide, and prostacyclin

Objective: The aim was to define: (1) whether bradykinin administration during reperfusion improves postischaemic myocardial recovery; (2) whether high energy phosphate compounds are involved in the protective effects of bradykinin; and (3) whether bradykinin-induced release of prostacyclin and nitric oxide mediate the protective effects of bradykinin. Methods: In the Langendorff rat heart preparation, coronary flow, left ventricular developed pressure, and, using 31P magnetic resonance spectroscopy, the high energy phosphate compounds phosphocreatine and β-ATP were assessed during 15 min of global ischaemia and 30 min of reperfusion. Administration of 10−7 M bradykinin was started before ischaemia and maintained throughout the experiment (BK-pre). This was compared to 10−7 M bradykinin given exclusively with reperfusion (BK-post). Then 10−7 M bradykinin was given simultaneously with 10−4 M Nω-nitro-L-arginine-methyl ester (BK-LNAME) or 10−5 M indomethacin (BK-indo). Results: In comparison to control hearts, BK-pre exerted a significant protective effect on the postischaemic recovery of coronary flow [71(5)% v 43(4)%, P < 0.05], left ventricular pressure [81(8)% v 42(5)%, P < 0.05], phosphocreatine [105(4)% v 67(8)%, P < 0.05], and β-ATP [78(9)% v 48(7)%, P < 0.05]. With BK-post, recovery of coronary flow [71(4)% v 43(4)%, P < 0.05] and left ventricular pressure [78(4)% v 42(5)%, P < 0.05] significantly improved; however the recovery of phosphocreatine [70(4)% v 67(8)%, NS] and β-ATP [58(2)% v 48(7)%, NS] was not different from control. When bradykinin and L-NAME or indomethacin was given the beneficial effects of bradykinin on ischaemic hearts were abolished. Conclusions: (1) Bradykinin improved postischaemic myocardial recovery when given before ischaemia or starting exclusively with reperfusion; (2) this was only partially related to a protective action on the high energy phosphate compounds during ischaemia; (3) the beneficial effects of bradykinin on ischaemic hearts are dependent from an unrestrained action of prostacyclin and nitric oxid