Pedestal Characteristics and MHD Stability of H-Mode Plasmas in TCV

The tokamak à configuration variable (TCV) is unique in its ability to create a variety of plasma shapes and to heat the electron population in high density regimes using microwave power at the third harmonic of the electron cyclotron frequency. In the frame of this thesis, the impact of plasma shaping and heating on the properties of the edge transport barrier (ETB) in the high confinement mode (H-mode) was studied. This mode of operation is foreseen as one of the reference scenarios for ITER, the International Tokamak Experimental Reactor, which is being built to demonstrate the feasibility of thermonuclear fusion using magnetic confinement. A feature of H-mode regime operation are edge localized modes (ELMs), instabilities driven by the steep pressure gradients that form in the plasma edge region due to a transport barrier. During an ELM event, energy and particles are expelled from the plasma in a short burst. This will cause serious problems with respect to the heat load on plasma facing components in a tokamak of the size of ITER. Understanding of the phenomena associated with ELMs is thus required and dedicated investigations of their theory and experimental observations are carried out in many laboratories worldwide. This thesis presents several experimental and numerical investigations of tokamak behavior for configurations where the plasma edge plays an important role. From the experimental viewpoint, studies of transport barriers are challenging, as plasma parameters change strongly within a narrow spatial region. As part of the work presented here, the TCV Thomson scattering system was upgraded to meet the requirements for diagnosing electron temperature and density with high spatial resolution in the region of internal and external transport barriers. Simultaneously, the data analysis was significantly improved to cope with statistical uncertainties and alleviate eventual systematic errors. For measurements of the time evolution of density and temperature profile during the ELM cycle, the low repetition rate of the lasers used for Thomson scattering is a limiting. Although the system on TCV comprises 3 laser units that may be triggered in sequence with time separations down to 1 ms, time evolution over longer periods can only be reconstructed from repetitive events. In this context, an adjustment of the laser trigger to improve the synchronization with the ELM event is an advantage. A method was developed and implemented to generate a synchronizing trigger sequence, by a real-time monitoring of the D-alpha emission, which provides a marker for the ELM event. Recently, a "snowflake" (SF) divertor configuration, proposed as a possible solution to reduce the plasma-wall interaction by changing the divertor's poloidal magnetic field topology, was generated, for the first time, in TCV. A numerical code (KINX), based on a magnetohydrodynamic model (ideal MHD), was used to investigate the stability limits of this configuration under H-mode conditions and compare them with a similar standard single-null equilibrium. In a series of experiments, improved energy confinement was found and explained by improved stability of the edge region in the SF configuration. The influence of the pedestal structure in ELMy H-mode plasmas on the energy confinement and on ELM energy losses was investigated. The different ELM regimes found in TCV were analyzed, in particular the transition between type-III to type-I ELMs. The operational boundary of each ELM regime was characterized and verified by ideal MHD stability simulations for the ETB region. Recent studies on the scaling of the pedestal width with normalized poloidal pressure were confirmed. Using the capabilities of TCV, the influence of plasma shaping on pedestal parameters and MHD stability limits was investigated. In the past, models were developed to describe the onset of type-I ELMs, which are associated with modes in the ETB region arising from a coupling of pressure- and current-driven instabilities (coupled kink-ballooning modes). Experimental studies were performed to trace the temporal evolution of pedestal parameters characterizing the ETB during an ELM cycle. The results of these experiments were analyzed using information from MHD stability calculations. It is concluded that these models are capable of predicting limits as necessary conditions for ELM activity, but are not sufficient to fully explain ELM triggering

Molecular dynamics in germinating, endophyte-colonized quinoa seeds

Aims: The pseudo-cereal quinoa has an outstanding nutritional value. Seed germination is unusually fast, and plant tolerance to salt stress exceptionally high. Seemingly all seeds harbor bacterial endophytes. This work examines mitogen-activated protein kinase (MAPK) activities during early development. It evaluates possible contribution of endophytes to rapid germination and plant robustness. Methods: MAPK activities were monitored in water- and NaCl-imbibed seeds over a 4-h-period using an immunoblot-based approach. Cellulolytic and pectinolytic abilities of bacteria were assessed biochemically, and cellular movement, biofilm, elicitor and antimicrobial compound synthesis genes sequenced. GyrA-based, cultivation-independent studies provided first insight into endophyte diversity. Results: Quinoa seeds and seedlings exhibit remarkably complex and dynamic MAPK activity profiles. Depending on seed origin, variances exist in MAPK patterns and probably also in endophyte assemblages. Mucilage-degrading activities enable endophytes to colonize seed surfaces of a non-host species, chia, without apparent adverse effects. Conclusions: Owing to their motility, cell wall-loosening and elicitor-generating abilities, quinoa endophytes have the potential to drive cell expansion, move across cell walls, generate damage-associated molecular patterns and activate MAPKs in their host. Bacteria may thus facilitate rapid germination and confer a primed state directly upon seed rehydration. Transfer into non-native crops appears both desirable and feasible.V167-B09(VLID)190449

Use of webcams as tools for alignment and supervision of a Thomson Scattering System in the near infrared

The alignment stability is a major concern for Thomson scattering systems. Even small angular deviations of the laser beams crossing the plasma lead to a loss of the calibration resulting in unreliable measurements of the electron density profile. For the TCV (Tokamak à Configuration Variable) installation, the beam paths from the laser output to the vacuum chamber are ∼25 m long and include several optical components. In order to monitor the alignment on a regular basis, a set of 9 cameras has been installed at several locations along the beam path. They view the actual laser beam pattern by recording the scattered light from an intercepting optical surface (mirror or window) together with the position of markers used for reference. Small webcams are used for this purpose; they feature adequate intensity response at the laser wavelength of 1.06 μm, are compact, cheap and several units may be connected to a server PC simultaneously. The real-time images from all the cameras are accessible from a Web browser. This installation has proven to be extremely useful in the early detection of alignment problems and to assist the alignment procedure [1] CRPP annual report (2005), p. 115.. [1]

Understanding the core density profile in TCV H-mode plasmas

Results from a database analysis of H-mode electron density profiles on the Tokamak \`a Configuration Variable (TCV) in stationary conditions show that the logarithmic electron density gradient increases with collisionality. By contrast, usual observations of H-modes showed that the electron density profiles tend to flatten with increasing collisionality. In this work it is reinforced that the role of collisionality alone, depending on the parameter regime, can be rather weak and in these, dominantly electron heated TCV cases, the electron density gradient is tailored by the underlying turbulence regime, which is mostly determined by the ratio of the electron to ion temperature and that of their gradients. Additionally, mostly in ohmic plasmas, the Ware-pinch can significantly contribute to the density peaking. Qualitative agreement between the predicted density peaking by quasi-linear gyrokinetic simulations and the experimental results is found. Quantitative comparison would necessitate ion temperature measurements, which are lacking in the considered experimental dataset. However, the simulation results show that it is the combination of several effects that influences the density peaking in TCV H-mode plasmas.Comment: 23 pages, 12 figure

Immunolocalization of dually phosphorylated MAPKs in dividing root meristem cells of Vicia faba, Pisum sativum, Lupinus luteus and Lycopersicon esculentum

Key message In plants, phosphorylated MAPKs display constitutive nuclear localization; however, not all studied plant species show co-localization of activated MAPKs to mitotic microtubules. Abstract The mitogen-activated protein kinase (MAPK) signaling pathway is involved not only in the cellular response to biotic and abiotic stress but also in the regulation of cell cycle and plant development. The role of MAPKs in the formation of a mitotic spindle has been widely studied and the MAPK signaling pathway was found to be indispensable for the unperturbed course of cell division. Here we show cellular localization of activated MAPKs (dually phosphorylated at their TXY motifs) in both interphase and mitotic root meristem cells of Lupinus luteus, Pisum sativum, Vicia faba (Fabaceae) and Lycopersicon esculentum (Solanaceae). Nuclear localization of activated MAPKs has been found in all species. Colocalization of these kinases to mitotic microtubules was most evident in L. esculentum, while only about 50 % of mitotic cells in the root meristems of P. sativum and V. faba displayed activated MAPKs localized to microtubules during mitosis. Unexpectedly, no evident immunofluorescence signals at spindle microtubules and phragmoplast were noted in L. luteus. Considering immunocytochemical analyses and studies on the impact of FR180204 (an inhibitor of animal ERK1/2) on mitotic cells, we hypothesize that MAPKs may not play prominent role in the regulation of microtubule dynamics in all plant species