Piscine UDP-glucuronosyltransferase 1B

Glucuronidation is an important detoxification pathway for organic pollutants in fish. We report here the isolation and characterisation of UDP-glucuronosyltransferases (UGT) genes from the closely related marine flatfish, plaice (Pleuronectes platessa) and flounder (Platichthys flesus). The deduced amino acid sequences share greater similarity with mammalian UGT1 family genes than UGT2 genes (44-47% and 39-40% amino acid identity respectively) and have been designated UGT1B. Both plaice and flounder UGT1B mRNAs are most highly expressed in liver, but are also expressed in intestine, gill, kidney and adipose tissue to a greater extent than muscle, heart or brain. Plaice UGT1B mRNA was undetectable in gametes or fertilised eggs and there was a large increase in expression between gastrulation and myotome formation after which levels declined some 5-10 fold. Flounder UGT1B mRNA was increased in liver after intraperitoneal injection of Arochlor 1254 or lindane, but not after perflourooctanoic acid or 3-methylcholanthrene. In isolated flounder hepatocytes UGT1B mRNA was increased by benzo(a)pyrene but not by ethynylestradiol. Expression of a cDNA for plaice UGT1B in cos7 cells resulted in higher 1-naphthol conjugation in cell homogenates compared to steroid conjugation, whilst bilirubin and bile acid conjugation undetectable. This indicates that the plaice gene codes for the phenol-conjugating UGT previously purified in our laboratory from this species and that it is likely to play a major role in the detoxification of polyaromatic hydrocarbons in flatfish. Its role in development is unknown. UGT1B genes are also present in pufferfish (Tetraodon nigroviridis) and zebrafish (Danio rerio) genomes, but that they differ in their genic organisation. Pufferfish possess multiple (repeated) complete UGT1 genes and Southern blots indicate that the homologous plaice UGT1B gene may also be organised in this way. In contrast, zebrafish appear to have two UGT1 loci whose sequences and intron/exon structures are closely related to that of plaice, however, the organisation of these genes is similar to the mammalian UGT1 family since each has multiple repeated exon 1’s which are alternatively spliced to a common set of exons encoding the aglycone binding domain. Taken together with evidence from phylogenetic comparison of fish sequences with UGT1 and UGT2 families in mammals, we suggest these homologous fish UGTs should all be included within the vertebrate UGT1 family and designated as UGT1B

IGC Antarctic Glaciological Data Field Work 1959.

"Submitted by Richard P. Goldthwait to the National Science Foundation, in partial fulfillment of Grant No. NSF-G8992."At head of title: "IGC Antarctic Glaciological Data Field Work 1959."This report presents the results of the glaciological program at Byrd Station during the winter of 1959 and the summer traverse of 1959-60. All field measurements were made by John Pirrit and George A. Doumani. While the glaciologists were on the Executive Range Traverse, 14 February to 6 March 1959, the thermohms at Byrd Station were read daily by H. LeVaux, the aurora physicist. During the summer traverse, the glaciologists were assisted in the snow pit work by E. Boudette, visiting USGS geologist. The glaciological data are given in four appendices.National Science Foundation Grant No. NSF-G8992

Development and Feasibility of a Low-Cost, Pacific Community-Focussed, Weight Management Programme in Glen Innes, Auckland

Background: Obesity is a significant nutritional public health issue in New Zealand, with 32%of adults (15 yrs+) classified as obese and a further 34% overweight but not obese [...

Functional analysis of the herpes simplex virus UL42 protein.

The herpes simplex virus UL42 gene encodes a multifunctional polypeptide (UL42) that is essential for virus DNA replication. To further understand the relationship between the structure of UL42 and the role that it plays during virus replication, we analyzed an extensive set of mutant UL42 proteins for the ability to perform the three major biochemical functions ascribed to the protein:binding to DNA, stably associating with the virus DNA polymerase (Pol), and acting to increase the length of DNA chains synthesized by Pol. Selected mutants were also assayed for their ability to complement the replication of a UL42 null virus. The results indicated that the N-terminal 340 amino acids of UL42 were sufficient for all three biochemical activities and could also support virus replication. Progressive C-terminal truncation resulted in the loss of detectable DNA-binding activity before Pol binding, while several mutations near the N terminus of the polypeptide resulted in an altered interaction with DNA but had no apparent affect on Pol binding. More dramatically, an insertion mutation at residue 160 destroyed the ability to bind Pol but had no effect on DNA binding. This altered polypeptide also failed to increase the length of DNA product synthesized by Pol, and the mutant gene could not complement the growth of a UL42 null virus, indicating that the specific interaction between Pol and UL42 is necessary for full Pol function and for virus replication. This study confirms the validity of the Pol-UL42 interaction as a target for the design of novel therapeutic agents

Assignment of the gene for dyskeratosis congenita to XQ28

Dyskeratosis congenita is an X linked recessive disorder with diagnostic dermatological features, bone marrow hypofunction, and a predisposition to neoplasia in early adult life. Linkage analysis was undertaken in an extensive family with the condition using the Xg blood group and 17 cloned X chromosomal DNA sequences which recognise restriction fragment length polymorphisms (RFLPs). No recombination was observed between the locus for dyskeratosis congenita (DKC) and the RFLPs identified by DXS52 (Stl4-1) (Zmax=3.33 at 8max=0 with 95% confidence limits of 0 to 14 cM). Similarly no recombination was observed for the disease locus and F8 (Zmax=1-23 at 8max=0) nor for DXSJ5 (DX13) (Zmax=1-62 at 8max=0), but both of these markers were only informative in part of the family whereas DXS52 was fully informative. DXS52, DXSJ5, and F8 are known to be tightly linked and have previously been assigned to Xq28. Thus the gene for dyskeratosis congenita can be assigned to Xq28. These DNA sequence polymorphisms will be of clinical value for carrier detection and prenatal diagnosis

Antarctic snow accumulation mapped using polarization of 4.3cm wavelength microwave emission

Different parts of Antarctica receive different amounts of snowfall each year. In this paper we map the variations of the mean annual snow accumulation across the ice sheet. We also quantify the uncertainty in our estimates more objectively than has been possible for earlier maps. The new map is produced using observations from satellites and ground-based measurements. After a logarithmic transformation, these are combined using the geostatistical method of continuous-part universal kriging to give an estimate of the snow accumulation within each cell of a rectangular grid covering Antarctica. We also derive spatial averages over the major drainage systems of the ice sheet, along with their confidence intervals. We obtain a value of 143 ± 4 kg m−2 a−1 for the average rate of snow accumulation upon the grounded ice sheet of Antarctica