Extensional flow of Newtonian and Boger fluids through a flow focusing microdevice

This paper was presented at the 3rd Micro and Nano Flows Conference (MNF2011), which was held at the Makedonia Palace Hotel, Thessaloniki in Greece. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, Aristotle University of Thessaloniki, University of Thessaly, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute.In this work we perform a numerical and experimental study on the flow of Newtonian and viscoelastic fluids through a microfluidic device in which hydrodynamic flow focusing is produced using two balanced lateral sheath streams that shape a third inlet stream. The flow focusing device used was conceived to achieve a nearly constant extensional rate along the centerline and is shaped much like a conventional cross-slot except for comprising three inlets and one exit channel. The work undertaken comprises experimental flow visualization as well as 2D and 3D numerical calculations using a finite volume method. The Newtonian fluid used in the experiments was distilled water and the viscoelastic fluid was an aqueous solution containing 125 ppm (w/w) of polyacrylamide (PAA, Mw = 18×10(6) g/mol), to which 1% of salt (NaCl) was added. The addition of salt to a shear-thinning PAA solution resulted in a low viscosity Boger fluid, i.e., a viscoelastic fluid with a nearly constant viscosity. The combination of small length scales characteristic of microfluidics with this type of fluid allows us to observe strong elastic effects in the absence of shear-thinning and inertial effects (or at least when these effects are weak). We report a rich variety of flow features, which depend on the fluid used as well as on the operational conditions. Additionally, we show good qualitative agreement between the experimental observations and the numerical predictions.FCT, FEDER and COMPETE for financial support through projects REEQ/262/EME/2005, REEQ/928/EME/2005 and PTDC/EME-MFE/099109/2008

Boger fluid flow through hyperbolic contraction microchannels

This paper was presented at the 3rd Micro and Nano Flows Conference (MNF2011), which was held at the Makedonia Palace Hotel, Thessaloniki in Greece. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, Aristotle University of Thessaloniki, University of Thessaly, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute.Boger fluids are characterized by their constant viscosity and elasticity and are very useful to study pure elastic flow behavior. In this paper we assess the potential of a microfluidic hyperbolic contraction as a device to measure the relaxation time of low viscosity polymer solutions, which are difficult to characterize in a conventional capillary break-up extensional rheometer. For this purpose we initially characterize the shear and extensional rheology of aqueous solutions of polyacrylamide (PAA) at different concentrations (400, 250, 125 and 50 ppm) with 1% (w/w) of NaCl, which result in low viscosity Boger fluids. Subsequently, flow visualizations of their flow through a microfluidic hyperbolic contraction were carried out in order to quantify the relation between their degree of elasticity and the vortex growth upstream of the microchannel.Fundação para a Ciência e a Tecnologia (FCT), COMPETE and FEDER through projects PTDC/ EQU-FTT/ 71800/2006, PTDC/EQUFTT/ 70727/2006, PTDC/EME-MFE/099109/ 2008, REEQ/928/EME/2005 and REEQ/298/EME/2005

Influence of channel aspect ratio on the onset of purely-elastic flow instabilities in three-dimensional planar cross-slots

In this work, we perform creeping-flow simulations of upper-convected Maxwell and simplified Phan-Thien-Tanner fluids to study the purely-elastic steady bifurcation and transition to time-dependent flow in three-dimensional planar cross-slots. By analysing the flow in geometries with aspect ratios ranging from the near Hele-Shaw flow like limit, up to the very deep, two-dimensional limit, we are able to characterize the mechanism of the cross-slot bifurcation with significant detail. We conclude that the bifurcation mechanism is similar to a buckling instability, by which fluid is redirected via paths of least resistance, resulting in the emergence of peripheral stagnation points, above and below the central stagnation point. The intake of matter at the centre via the inlet axis is thus reduced, being compensated by fluid flowing through low resistance corridors along the central vertical axis, above and below the central point. Furthermore, we propose and locally compute a modified Pakdel-McKinley criterion, thereby producing a scalar stability field and suggesting emergent peripheral stagnation points also indirectly contribute to the onset of time-dependent flow. (c) 2015 The Authors. Published by Elsevier B.V

A new viscoelastic benchmark flow: Stationary bifurcation in a cross-slot

AbstractIn this work we propose the cross-slot geometry as a candidate for a numerical benchmark flow problem for viscoelastic fluids. Extensive data of quantified accuracy is provided, obtained via Richardson extrapolation to the limit of infinite refinement using results for three different mesh resolutions, for the upper-convected Maxwell, Oldroyd-B and the linear form of the simplified Phan-Thien–Tanner constitutive models. Furthermore, we consider two types of flow geometry having either sharp or rounded corners, the latter with a radius of curvature equal to 5% of the channel’s width. We show that for all models the inertialess steady symmetric flow may undergo a bifurcation to a steady asymmetric configuration, followed by a second transition to time-dependent flow, which is in qualitative agreement with previous experimental observations for low Reynolds number flows. The critical Deborah number for both transitions is quantified and a set of standard parameters is proposed for benchmarking purposes

Microchannels analogues for the study of viscoelastic fluid flows through porous media

This paper was presented at the 3rd Micro and Nano Flows Conference (MNF2011), which was held at the Makedonia Palace Hotel, Thessaloniki in Greece. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, Aristotle University of Thessaloniki, University of Thessaly, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute.This work studies the flow behavior and related pressure losses of viscoelastic polymer solutions in microchannels with two different sequences of contraction/expansion, disposed in a symmetric and an asymmetric arrangement, respectively. These microfluidic devices are proposed as simplified microchannel analogues for the flow of Newtonian and viscoelastic fluids through porous media. The results show that the symmetric configuration mimics the pressure gradient of these polymer solutions through a porous medium at low flow rates (below a critical Deborah number, Decr), while the asymmetric arrangement gives the asymptotic limit at high De values (above Decr) as a consequence of the intrinsic differences in the extensional rate profiles defined by each microgeometry.Fundação para a Ciência e a Tecnologia (FCT), COMPETE and FEDER through projects PTDC/ EQU-FTT/ 71800/ 2006, PTDC/EQUFTT/ 70727/ 2006, PTDC/ EME-MFE/ 99109/ 2008 and REEQ/ 262/ EME/ 2005

Glycan Structures Contain Information for the Spatial Arrangement of Glycoproteins in the Plasma Membrane

Glycoconjugates at the cell surface are crucial for cells to communicate with each other and the extracellular microenvironment. While it is generally accepted that glycans are vectorial biopolymers, their information content is unclear. This report provides evidence that distinct N-glycan structures influence the spatial arrangement of two integral membrane glycoproteins, Kv3.1 and E-cadherin, at the adherent membrane which in turn alter cellular properties. Distinct N-glycan structures were generated by heterologous expression of these glycoproteins in parental and glycosylation mutant Chinese hamster ovary cell lines. Unlike the N-linked glycans, the O-linked glycans of the mutant cell lines are similar to those of the parental cell line. Western and lectin blots of total membranes and GFP immunopurified samples, combined with glycosidase digestion reactions, were employed to verify the glycoproteins had predominantly complex, oligomannose, and bisecting type N-glycans from Pro(-)5, Lec1, and Lec10B cell lines, respectively. Based on total internal reflection fluorescence and differential interference contrast microscopy techniques, and cellular assays of live parental and glycosylation mutant CHO cells, we propose that glycoproteins with complex, oligomannose or bisecting type N-glycans relay information for localization of glycoproteins to various regions of the plasma membrane in both a glycan-specific and protein-specific manner, and furthermore cell-cell interactions are required for deciphering much of this information. These distinct spatial arrangements also impact cell adhesion and migration. Our findings provide direct evidence that N-glycan structures of glycoproteins contribute significantly to the information content of cells

Exploring the role of individual level and firm level dynamic capabilities in SMEs’ internationalization

This paper presents a multi-level model that examines the impact of dynamic capabilities on the internationalization of SMEs while taking into account the interactions among them. The purpose of the research is to understand the applicability of dynamic capabilities at the individual and the firm level to the SME internationalization process in developing country context and to assess to what extent a firm’s asset position and individual level dynamic capabilities influence the generation of firm level dynamic capabilities in SMEs. First, the dynamic capabilities theory was theoretically linked to the internationalization phenomenon. The relationships among firm-level dynamic capabilities, individual-level dynamic capabilities (owner specific dynamic capabilities), and internationalization were identified. The research framework and hypotheses were developed and empirically tested with 197 SMEs. The findings established that owner-specific dynamic capabilities have a positive influence on both firm dynamic capabilities and internationalization, and firm dynamic capabilities positively influence internationalization. It was also found that the market assets position measured as perceptual environmental dynamism positively influenced firm dynamic capabilities but structural and reputational asset positions of SMEs did not influence generation of firm dynamic capabilities. Moreover, firm dynamic capabilities had a mediation effect in the relationship between owner-specific dynamic capabilities and internationalization. Theoretically, this confirms the relevance of dynamic capability theory to internationalization and the possibility of integrating existing internationalization theories. Entrepreneurs, SME managers, and policy-makers could gain valuable insights on how entrepreneur and firm capabilities lead to better international prospects from this outcome