Avaliação da infiltração apical nas obturações de canais radiculares preparados com uma técnica manual e outra rotatória

Some of the improvements in endodontics in the latest years are the Nickel Titanium instruments as well as the different instrumentation techniques developed with rotary systems. In this study it was performed a comparison between the levels of apical microleakage of dye blue methylene, in superior premolars with twin, divergents roots, and completely closed apex where one of the roots was handled with the manual telescopic technique, and the other one with the Profile System. (Maillefer). K-Flexofile and files of the Profile System were used during the instrumentation process; ENDO-PTC was used as an auxiliary during the instrumentation. The irrigation was made with sodium hypochlorite at 1% and Tergensol for final flushing. Gutta-percha points and Endofill cement were the obturation choice, using lateral condensation technique. Apical infiltration was assessed by linear penetration on the stereomicroscope by two examiners. The results shown, that apical microleakage occurred, with hand or rotary technique. It was noticed that infiltration levels were superior in the sample instrumented with the rotary technique. In these teeth the apical matrix was handled with a smaller quantity of instruments. Statistically, infiltration values between the two groups was not significant (p= 0.0753).Alguns dos avanços na endodontia, nos últimos anos, referem-se aos instrumentos de Níquel Titânio, assim como às diferentes técnicas de instrumentação desenvolvidas com instrumentos rotatórios. Neste estudo, foi realizada uma comparação nos níveis de infiltração apical de corante azul de metileno em pré-molares superiores, birradiculares, com raízes divergentes e ápice completamente fechado, quando uma das raízes foi instrumentada com a técnica escalonada e a outra raiz com o Sistema Profile, (Maillefer). Os dentes foram instrumentados com limas K-Flexofile e limas do sistema Profile, ENDO-PTC foi auxiliar da instrumentação. Foram irrigados com hipoclorito de sódio a 1 % e Tergensol como irrigante final, e obturados com cones de guta-percha e cimento Endofill, usando-se a técnica de condensação lateral. A infiltração apical foi avaliada por medida linear, em lupa estereoscópica, por dois examinadores. Os resultados mostraram que, independentemente da técnica de instrumentação utilizada, aconteceu infiltração. Observou-se que os maiores níveis de infiltração aconteceram nas amostras que foram instrumentadas com a técnica rotatória. Nesses dentes, a matriz apical não foi conformada. Estatisticamente, os valores de infiltração entre os grupos experimentais não foram significantes (p= 0.0753)

Conocimientos y actitudes sobre vacuna del virus del papiloma humano, de profesionales en salud que brindan atención materno infantil. Unidad Comunitaria de Salud Familiar Santo Tomás y Ciudad Delgado. Octubre 2014

A nivel mundial el cáncer cervico uterino es una de las principales causas de mortalidad en la población femenina, en El Salvador anualmente se confirman más de mil casos de dicha enfermedad, siendo el Virus del Papiloma Humano el agente etiológico más frecuente. A raíz de esta problemática la Organización Mundial de la Salud ha venido desarrollando desde la década de los 80, la vacuna contra el Virus del Papiloma Humano. En varios países como Argentina y Brasil esta vacuna ya se incorporó a su esquema nacional de vacunación sin embargo en nuestro país aún no ha sido introducida por múltiples factores, a pesar de los esfuerzos realizados por distintas organizaciones. Aunque existe información sobre la vacuna que previene la infección, no se encontraron estadísticas sobre conocimientos que poseen los-as profesionales en salud. Es por ello que la presente investigación tuvo como objetivo analizar los conocimientos y actitudes sobre vacuna del Virus del Papiloma Humano de profesionales en salud que brindan atención a los programas materno infantil en las Unidades Comunitarias de Salud Familiar de Santo Tomás y Ciudad Delgado

Comparison of peak pressure in flexible cushions in spinal cord injured and normal subjects: an interface pressure evaluation

As úlceras por pressão (UP) são complicações freqüentes em pacientes com lesão medular (LM). Estratégias de prevenção objetivam reduzir a magnitude ou duração da pressão, fricção e cisalhamento entre a pele e superfície de suporte. Para medir a eficácia destas superfícies utilizam-se medidas de interface de pressão. O objetivo deste estudo é determinar os índices de interface de pressão em assento flexível em pacientes lesados medulares. Um estudo transversal com 103 indivíduos com LM e 101 pessoas sadias foi realizado com o mapeamento dos picos de pressão por meio de um sistema de interface sensível em cadeira de rodas padrão, com almofada flexível. Dados antropométricos foram obtidos pelo exame físico para o cálculo do índice de massa corpórea (IMC). Os resultados confirmam os elevados índices de interface de pressão na amostra de pacientes com LM. Com o aumento do IMC em indivíduos não lesados medulares há uma diminuição dos picos de pressão, ou seja, melhor distribuição da força. Na amostra de pacientes com LM, observou-se que apesar do aumento do IMC, a maioria dos indivíduos mantém elevados índices de pressão na posição sentada. Não é possível contestar os estudos que relacionam picos de pressão, lesão medular e peso, pois na amostra estudada, indivíduos obesos ou com sobrepeso apresentam pequena representação.Pressure sores are frequent complications of spinal cord injured (SCI) patients. Preventive strategies aim at reducing the intensity or duration of pressure, friction and splitting forces between the skin and the supporting surface. The aim of the study is to determine indexes of interface pressure in flexible cushions for SCI patients. A cross sectional study with 103 and 101 able-bodied persons was carried with the mapping of pressure peaks by means of a sensible interface system, with patients seated in a standard wheelchair and using flexible cushions. Antropometric data obtained during physical to determine the body mass index (BMI). Our results confirm that the elevated levels of interface pressure in SCI patients. The increase in BMI in non SCI individuals resulted in reduction of interface peak pressure, indicating a better distribution of force. Those SCI patients showed increased levels of interface peak pressure in the seated position, despite the values of BMI. It is not possible to contest the results of other studies that relate pressure peaks, SCI and body weight, because there were few overweight or obese patients in the studied sample

Effect of Spatiotemporal Parameters on the Gait of Children Aged from 6 to 12 Years in Podiatric Tests: A Cross Sectional Study

The use of lower limb tests in the paediatric population is of great importance for diagnostic evaluations. The aim of this study is to understand the relationship between the tests performed on the feet and ankles, covering all of its planes, and the spatiotemporal parameters of children’s gait. Methods: It is a cross-sectional observational study. Children aged between 6 and 12 years participated. Measurements were carried out in 2022. An analysis of three tests used to assess the feet and ankles (FPI, the ankle lunge test, and the lunge test), as well as a kinematic analysis of gait using OptoGait as a measurement tool, was performed. Results: The spatiotemporal parameters show how Jack’s Test is significant in the propulsion phase in its % parameter, with a p-value of 0.05 and a mean difference of 0.67%. Additionally, in the lunge test, we studied the % of midstance in the left foot, with a mean difference between the positive test and the 10 cm test of 10.76 (p value of 0.04). Conclusions: The diagnostic analysis of the functional limitation of the first toe (Jack’s test) is correlated with the spaciotemporal parameter of propulsion, as well as the lunge test, which is also correlated with the midstance phase of gait.Funding for open access charge: Universidad de Málag

Follow-up of health-related quality of life and pain in a cohort of patients with rheumatoid arthritis before and after COVID-19

The foot is one of the anatomical structures of the body most affected in rheumatoid arthritis (RA), associated with the disability of patients, even more during COVID-19. The aim of this study was to analyse whether the period of physical inactivity during COVID-19 is an influential factor on health-related quality of life and foot pain in patients with RA. Methods: 162 patients with foot pain and RA, recruited from the Hospital Virgen de las Nieves, Granada (Spain) were included. Data was collected during two different periods: January - December 2018 in person and June - September 2021 by phone. Patients were asked to complete the Spanish adapted version of the 12-Item Short Form Survey (SF-12) and the Visual Analogue Scale (VAS). Results: The results from the SF-12 questionnaires were divided between its two subscales (i.e., mental, and physical component). The physical component shows an improvement between 2018 and 2021, from 32.05in 2018–35.18 in 2021 (p < 0.05). The opposite happened with the mental component, showing a dete- rioration, from 39.69 in 2018–34.48 in 2021 (p < 0.05). Regarding pain, VAS shows higher levels of pain with statistically significant differences, both in general pain (from 6 in 2018–7 in 2012) and in foot pain (from 5 to 7), (p < 0.05). Conclusion: Mental quality of life and pain, both general and foot pain, are influenced by the period of physical inactivity during COVID-19.Funding for open access charge: Universidad de Málaga / CBU

Computational Tools for Splicing Defect Prediction in Breast/Ovarian Cancer Genes: How Efficient Are They at Predicting RNA Alterations?

In silico tools for splicing defect prediction have a key role to assess the impact of variants of uncertain significance. Our aim was to evaluate the performance of a set of commonly used splicing in silico tools comparing the predictions against RNA in vitro results. This was done for natural splice sites of clinically relevant genes in hereditary breast/ovarian cancer (HBOC) and Lynch syndrome. A study divided into two stages was used to evaluate SSF-like, MaxEntScan, NNSplice, HSF, SPANR, and dbscSNV tools. A discovery dataset of 99 variants with unequivocal results of RNA in vitro studies, located in the 10 exonic and 20 intronic nucleotides adjacent to exon–intron boundaries of BRCA1, BRCA2, MLH1, MSH2, MSH6, PMS2, ATM, BRIP1, CDH1, PALB2, PTEN, RAD51D, STK11, and TP53, was collected from four Spanish cancer genetic laboratories. The best stand-alone predictors or combinations were validated with a set of 346 variants in the same genes with clear splicing outcomes reported in the literature. Sensitivity, specificity, accuracy, negative predictive value (NPV) and Mathews Coefficient Correlation (MCC) scores were used to measure the performance. The discovery stage showed that HSF and SSF-like were the most accurate for variants at the donor and acceptor region, respectively. The further combination analysis revealed that HSF, HSF+SSF-like or HSF+SSF-like+MES achieved a high performance for predicting the disruption of donor sites, and SSF-like or a sequential combination of MES and SSF-like for predicting disruption of acceptor sites. The performance confirmation of these last results with the validation dataset, indicated that the highest sensitivity, accuracy, and NPV (99.44%, 99.44%, and 96.88, respectively) were attained with HSF+SSF-like or HSF+SSF-like+MES for donor sites and SSF-like (92.63%, 92.65%, and 84.44, respectively) for acceptor sites.We provide recommendations for combining algorithms to conduct in silico splicing analysis that achieved a high performance. The high NPV obtained allows to select the variants in which the study by in vitro RNA analysis is mandatory against those with a negligible probability of being spliceogenic. Our study also shows that the performance of each specific predictor varies depending on whether the natural splicing sites are donors or acceptors

A Phase I-II multicenter trial with Avelumab plus autologous dendritic cell vaccine in pre-treated mismatch repair-proficient (MSS) metastatic colorectal cancer patients; GEMCAD 1602 study

[Background]: Immune check-point blockade (ICB) has shown clinical beneft in mismatch repair-defcient/microsatellite instability high metastatic colorectal cancer (mCRC) but not in mismatch repair-profcient/microsatellite stable patients. Cancer vaccines with autologous dendritic cells (ADC) could be a complementary therapeutic approach to ICB as this combination has the potential to achieve synergistic efects. [Methods]: This was a Phase I/II multicentric study with translational sub-studies, to evaluate the safety, pharmacodynamics and anti-tumor efects of Avelumab plus ADC vaccine in heavily pre-treated MSS mCRC patients. Primary objective was to determine the maximum tolerated dose and the efcacy of the combination. The primary end-point was 40% progressionfree survival at 6 months with a 2 Simon Stage. [Results]: A total of 28 patients were screened and 19 pts were included. Combined therapy was safe and well tolerated. An interim analysis (Simon design frst-stage) recommended early termination because only 2/19 (11%) patients were disease free at 6 months. Median PFS was 3.1 months [2.1–5.3 months] and overall survival was 12.2 months [3.2–23.2 months]. Stimulation of immune system was observed in vitro but not clinically. The evaluation of basal RNA-seq noted signifcant changes between pre and post-therapy liver biopsies related to lipid metabolism and transport, infammation and oxidative stress pathways. [Conclusions]: The combination of Avelumab plus ADC vaccine is safe and well tolerated but exhibited modest clinical activity. Our study describes, for the frst-time, a de novo post-therapy metabolic rewiring, that could represent novel immunotherapyinduced tumor vulnerabilities.The study was funded by grants from the FIS PI17/00732 from Instituto de Salud Carlos III, Premi Fi de Residència Emili Letang from Hospital Clínic Barcelona, Plan Nacional de I + D (PID-107139RB-C21 to DB-R and PID2020-115051RB-I00 to MC) and Grupo Español Multidisciplinar en Cáncer Digestivo (GEMCAD). The study was funded with Grants from Catalan Agency for Management of University and Research Grants (AGAUR) (2014-SGR-474, 2017-SGR-1174 and 2017-SGR-1033), Fundació la Marató de TV3 (201330.10), Instituto de Salud Carlos III (PI13/01728 and PI19/00740) and Fundacion Olga Torres (Modalitat A. 2019/2020) to JM. IMMETCOLS signature is under patent protection (EP21382772.8.) This research was financially supported by GEMCAD and (OR Avelumab was provided) by Merck, S.L.U., Madrid, Spain, an affiliate of Merck KGaA, Darmstadt, Germany, as part of an alliance between the healthcare business of Merck KGaA, Darmstadt, Germany (CrossRef Funder ID: https://doi.org/10.13039/100009945) and Pfizer

Las Matemáticas Empresariales en el marco Erasmus Mundus

La creciente importancia de los métodos cuantitativos en las ciencias económicas y empresariales nos motiva a proponer una revisión detallada de los syllabus de las materias de matemáticas que se imparten en el Grado de Administración y Dirección de Empresas, con el objetivo de Investigar las correspondencias entre nuestros syllabus y los de las mas importantes universidades a nivel internacional (en el marco Erasmus Mundus). La investigación que proponemos llevará a cabo comparaciones exhaustivas de los temarios de esta categoría de asignaturas y sus metodologías docentes, y detectará las posibles discrepancias existentes en este tipo de estudios dependiendo de la universidad que los imparte. En una segunda fase, estudiaremos las causas de las posibles diferencias detectadas y, finalmente, produciremos un sistema capaz de sugerir medidas concretas que solventen los posibles problemas detectados

Análisis de los contenidos docentes de matemáticas en el doble grado ADE-Informática

Depto. de Economía Financiera y Actuarial y EstadísticaFac. de Ciencias Económicas y EmpresarialesFALSEsubmitte