Fungos endofíticos em folhas de Erva-mate (Ilex paraguariensis A. St.-Hill.).

Este estudo teve como objetivo analisar o efeito da idade de folhas de erva-mate, colhidas em ervas cultivadas e nativas, sobre a população de fungos endofíticos. Como resultado verificou-se que ocorre uma maior diversidade de fungos endofíticos em folhas adultas de erva-mate do que em folhas jovens. A diversidade foi maior nas plantas nativas, com uma microbiota mais abundante, decorrente de um ambiente sombreado e úmido, mais propício para o seu desenvolvimento, diferente de um agroecossistema. Plantas de erva-mate nativa apresentaram fungos endofíticos de gêneros com potencial de biocontrole que deveriam ser testados quanto à capacidade de induzir proteção contra pragas e doenças.Notas técnicas

Magnetic Properties of Weakly Doped Antiferromagnets

We study the spin excitations and the transverse susceptibility of a two-dimensional antiferromagnet doped with a small concentration of holes in the t-J model. The motion of holes generates a renormalization of the magnetic properties. The Green's functions are calculated in the self-consistent Born approximation. It is shown that the long-wavelength spin waves are significantly softened and the shorter-wavelength spin waves become strongly damped as the doping increases. The spin wave velocity is reduced by the coherent motion of holes, and not increased as has been claimed elsewhere. The transverse susceptibility is found to increase considerably with doping, also as a result of coherent hole motion. Our results are in agreement with experimental data for the doped copper oxide superconductors.Comment: 20 page

Neuronal deletion of GSK3beta increases microtubule speed in the growth cone and enhances axon regeneration via CRMP-2 and independently of MAP1B and CLASP2

BACKGROUND: In the adult central nervous system, axonal regeneration is abortive. Regulators of microtubule dynamics have emerged as attractive targets to promote axonal growth following injury as microtubule organization is pivotal for growth cone formation. In this study, we used conditioned neurons with high regenerative capacity to further dissect cytoskeletal mechanisms that might be involved in the gain of intrinsic axon growth capacity. RESULTS: Following a phospho-site broad signaling pathway screen, we found that in conditioned neurons with high regenerative capacity, decreased glycogen synthase kinase 3β (GSK3β) activity and increased microtubule growth speed in the growth cone were present. To investigate the importance of GSK3β regulation during axonal regeneration in vivo, we used three genetic mouse models with high, intermediate or no GSK3β activity in neurons. Following spinal cord injury, reduced GSK3β levels or complete neuronal deletion of GSK3β led to increased growth cone microtubule growth speed and promoted axon regeneration. While several microtubule-interacting proteins are GSK3β substrates, phospho-mimetic collapsin response mediator protein 2 (T/D-CRMP-2) was sufficient to decrease microtubule growth speed and neurite outgrowth of conditioned neurons and of GSK3β-depleted neurons, prevailing over the effect of decreased levels of phosphorylated microtubule-associated protein 1B (MAP1B) and through a mechanism unrelated to decreased levels of phosphorylated cytoplasmic linker associated protein 2 (CLASP2). In addition, phospho-resistant T/A-CRMP-2 counteracted the inhibitory myelin effect on neurite growth, further supporting the GSK3β-CRMP-2 relevance during axon regeneration. CONCLUSIONS: Our work shows that increased microtubule growth speed in the growth cone is present in conditions of increased axonal growth, and is achieved following inactivation of the GSK3β-CRMP-2 pathway, enhancing axon regeneration through the glial scar. In this context, our results support that a precise control of microtubule dynamics, specifically in the growth cone, is required to optimize axon regrowth

Ostrogradski Formalism for Higher-Derivative Scalar Field Theories

We carry out the extension of the Ostrogradski method to relativistic field theories. Higher-derivative Lagrangians reduce to second differential-order with one explicit independent field for each degree of freedom. We consider a higher-derivative relativistic theory of a scalar field and validate a powerful order-reducing covariant procedure by a rigorous phase-space analysis. The physical and ghost fields appear explicitly. Our results strongly support the formal covariant methods used in higher-derivative gravity.Comment: 22 page

Quantum Cosmology in Scalar-Tensor Theories With Non Minimal Coupling

Quantization in the minisuperspace of non minimal scalar-tensor theories leads to a partial differential equation which is non separable. Through a conformal transformation we can recast the Wheeler-DeWitt equation in an integrable form, which corresponds to the minimal coupling case, whose general solution is known. Performing the inverse conformal transformation in the solution so found, we can construct the corresponding one in the original frame. This procedure can also be employed with the bohmian trajectories. In this way, we can study the classical limit of some solutions of this quantum model. While the classical limit of these solutions occurs for small scale factors in the Einstein's frame, it happens for small values of the scalar field non minimally coupled to gravity in the Jordan's frame, which includes large scale factors.Comment: latex, 18 page

Magnetic field chaos in the SK Model

We study the Sherrington--Kirkpatrick model, both above and below the De Almeida Thouless line, by using a modified version of the Parallel Tempering algorithm in which the system is allowed to move between different values of the magnetic field h. The behavior of the probability distribution of the overlap between two replicas at different values of the magnetic field h_0 and h_1 gives clear evidence for the presence of magnetic field chaos already for moderate system sizes, in contrast to the case of temperature chaos, which is not visible on system sizes that can currently be thermalized.Comment: Latex, 16 pages including 20 postscript figure