Plant Community Assessment and Management Recommendations for Minneapolis Park Natural Areas

In 2017 the Minneapolis Park and Recreation Board (MPRB) began a two-phase study to collect quantitative and qualitative data for urban park natural areas in Minneapolis, MN parks to inform management activities. The first phase took existing GIS data and quality ranking systems and tailored them to the Minneapolis park system. The second phase, which is still in process, involves field checking the data, applying the quality ranking system, and writing a management plan

Evidence that the C-terminal PB2-binding region of the influenza A virus PB1 protein is a discrete alpha-helical domain

AbstractThe influenza A virus RNA-dependent RNA polymerase is a heterotrimer composed of PB1, PB2 and PA subunits and essential for viral replication. However, little detailed structural information is available for this important enzyme. We show by circular dichroism spectroscopy that polypeptides from the C-terminus of PB1 that are capable of binding efficiently to PB2 fold into stable α-helical structures. Structure prediction analysis of this region of PB1 indicates that it likely consists of a three-helical bundle. Deletion of any of the helices abrogated transcriptional function. Thus, PB1 contains a C-terminal α-helical PB2-binding domain that is essential for nucleotide polymerization activity