Marine bacterial inhibitors from the sponge-derived fungus Aspergillus sp

Chromatographic separation of a crude extract obtained from the fungus Aspergillus sp., isolated from the Mediterranean sponge Tethya aurantium, yielded a new tryptophan derived alkaloid, 34(1-hydroxy-3-(2methylbut-3-en-2-y1)-2-oxoindolin-3-yl)methyl)-1-methyl-3,4-dihydrobenzo[e][1,41diazepine-2, 5-dione (1), and a new meroterpenoid, austalide R (2), together with three known compounds (3-5). The structures of the new compounds were unambiguously elucidated on the basis of extensive one and twodimensional NMR (1H, 13C, COSY, HMBC, and ROESY) and mass spectral analysis. Interestingly, the compounds exhibited antibacterial activity when tested against a panel of marine bacteria, with 1 selectively inhibiting Vibrio species and 2 showing a broad spectrum of activity. In contrast, no significant activity was observed against terrestrial bacterial strains and the murine cancer cell line L5178Y. (C) 2014 Elsevier Ltd. All rights reserved.Chemistry, OrganicSCI(E)[email protected]

Bodies Moving and Being Moved: Mapping affect in Christian Nold's Bio Mapping

In A History of Spaces (2004), John Pickles observes that one of the less well-known representational norms of mapping is its focus on ‘natural and physical objects rather than developing universal conventions dealing with symbol, affect and movement.’ New media artist Christian Nold's work has dealt explicitly with two of these cartographic blindspots, grafting new and old technologies that both, in different ways, create bodily traces – the GPS trace of movement and the GSR (galvanic skin response) trace of arousal, often taken as an index of emotional response. Although Nold's socially engaged practice can be placed within the ‘locative media’ genre it also taps into the technological imaginaries around physiological sensors and intimate data. This paper considers Nold's Bio Mapping (2004-) projects in the context of his longstanding concern with social collectives and public space as a field of social relations. Looking at particular maps from Nold's Bio Mapping project, it considers the implications of blending the traces of the body's internal states with the traces produced by locomotive movement, and the relationship between the individuals thus traced and the collectives that Nold seeks to represent. Concurrent with Nold's practice there has been a wave of interest in affect and emotion (and the distinction between them) within the humanities. This paper brings Nold's work into contact with the Deleuzian/Spinozan concept of affect employed in one strand of this writing, drawing in particular on the work of Brian Massumi. Rather than using theory to simply illustrate Nold's practice, it follows the implications of Deleuze's cartographic model of individuation, the logic of which ultimately problematises the very distinction between the two bodily phenomena traced by Nold's device

Evaluation of cationic micropeptides derived from the innate immune system as inhibitors of marine biofouling

WOS:000356074000007A series of 13 short synthetic amphiphilic cationic micropeptides, derived from the antimicrobial iron-binding innate defence protein lactoferrin, have been evaluated for their capacity to inhibit the marine fouling process. The whole biofouling process was studied and microfouling organisms such as marine bacteria and microalgae were included as well as the macrofouling barnacle Balanus improvisus. In total 19 different marine fouling organisms (18 microfoulers and one macrofouler) were included and both the adhesion and growth of the microfoulers were investigated. It was shown that the majority of the peptides inhibited barnacle cyprid settlement via a reversible nontoxic mechanism, with IC50 values as low as 0.5 mu g ml(-1). Six peptides inhibited adhesion and growth of microorganisms. Two of these were particularly active against the microfoulers with MIC-values ranging between 0.01 and 1 mu g ml(-1), which is comparable with the commercial reference antifoulant SeaNine

The effectiveness of disinfectant and steam exposure treatments to prevent the spread of the highly invasive killer shrimp, Dikerogammarus villosus

Biosecurity protocols designed to prevent the spread of invasive alien species (IAS) are now an essential aspect of IAS management. However, the effectiveness of various biosecurity treatments requires further exploration. Killer shrimp, Dikerogammarus villosus, a notoriously high impact and ecosystem destabilising invader, has rapidly spread across Europe, and is of concern to invade Northern America. In this study, we examine the effectiveness of three commonly used, broad-spectrum disinfectants to cause mortality of D. villosus: Virasure Aquatic, Virkon Aquatic, and Virkon S. Immersion and spray treatments of 1%, 2% and 4% disinfectant solutions were examined for applications of up to 300 secs immersion and for up to ten consecutive sprays. Furthermore, we assessed the effectiveness of steam (≥100 °C) treatments for up to 120 secs. For all disinfectants, immersion in 1% solutions caused 100% mortality at ≥120 secs. At higher concentrations, shorter immersion times caused complete mortality: 60 and 15 secs for 2% and 4% solutions, respectively. Five sprays of 2% and 4% solutions resulted in 100% mortality, for all disinfectants. Direct steam exposure was highly effective, with complete D. villosus mortality occurring at ≥10 secs. Overall, brief exposure to broad-spectrum disinfectants and direct steam could be used to limit D. villosus spread