In vitro maturation of immature thymocytes into immunocompetent T cells in the absence of direct thymic influence

Peanut lectin (PNL) binds to a majority of mouse thymocytes (Thc) in suspension. By using cell affinity chromatography on a column of anti-PNL antibody, Thc populations at least 96 percent pure in PNL + or - cells, as judged by immunofluorescence, were obtained. PNL(+) cells are rich in Thy 1 and poor in H(2) antigens, cortisone sensitive, unresponsive to phytohemagglutinin (PHA), and immunologically incompetent, as judged by mixed lymphocyte reaction, popliteal lymph node graft-versus-host assay, and by testing helper activity in a primary in vitro antibody response to sheep erythrocytes; the converse is true of PNL(-) cells. Thus, PNL(+) and (-) cells appear to correspond to cortical and medullary Thc, respectively, as previously suggested. In culture, PNL(+) Thc show poor viability and a weak proliferative response to concanavalin A (Con A), except when supernate (SUP) of 24 h Con A stimulated lymph node lymphocyte cultures, or irradiated lymph node cells, are added, in which cases a strong proliferative response to the mitogen is observed. A variety of control experiments showed that the proliferating cells did not result from preferential stimulation of a few contaminating PNL(-) Thc present in the PNL(+) Thc cultures. The blasts resulting from PNL(+) Thc proliferation display mitogen-induced cytotoxicity, and give rise to a population of medium-sized lymphocytes, mostly PNL(-), poor in Thy 1 and rich in H(2) antigens, PHA responsive, and immunologically competent in the above-mentioned assays. Fresh PNL(+) Thc responded in mixed lymphocyte reaction in the presence of SUP (lectin depleted) and since incubation in SUP alone did not confer reactivity on PNL(+) Thc, it appears therefore that (a) immature Thc possess alloantigen and mitogen-specific surface receptors but lack the capacity to respond by proliferation to receptor triggering without the help of extracellular factor(s) released by mature lymphoid cells stimulated by mitogens (b) cell division is associated with the acquisition of immunological responsiveness, characteristic of mature T lymphocytes. The implications of these findings for the ontogenesis of thymus-derived lymphocytes, and for the possible traffic of Thc within and from the thymus, are discussed

UV finiteness of 3D Yang-Mills theories with a regulating mass in the Landau gauge

We prove that three-dimensional Yang-Mills theories in the Landau gauge supplemented with a infrared regulating, parity preserving mass term are ultraviolet finite to all orders. We also extend this result to the Curci-Ferrari gauge.Comment: 6 page

Observables in Topological Yang-Mills Theories

Using topological Yang-Mills theory as example, we discuss the definition and determination of observables in topological field theories (of Witten-type) within the superspace formulation proposed by Horne. This approach to the equivariant cohomology leads to a set of bi-descent equations involving the BRST and supersymmetry operators as well as the exterior derivative. This allows us to determine superspace expressions for all observables, and thereby to recover the Donaldson-Witten polynomials when choosing a Wess-Zumino-type gauge.Comment: 39 pages, Late

On the symmetries of BF models and their relation with gravity

The perturbative finiteness of various topological models (e.g. BF models) has its origin in an extra symmetry of the gauge-fixed action, the so-called vector supersymmetry. Since an invariance of this type also exists for gravity and since gravity is closely related to certain BF models, vector supersymmetry should also be useful for tackling various aspects of quantum gravity. With this motivation and goal in mind, we first extend vector supersymmetry of BF models to generic manifolds by incorporating it into the BRST symmetry within the Batalin-Vilkovisky framework. Thereafter, we address the relationship between gravity and BF models, in particular for three-dimensional space-time.Comment: 29 page

Let's Twist Again: N=2 Super Yang Mills Theory Coupled To Matter

We give the twisted version of N=2 Super Yang Mills theory coupled to matter, including quantum fields, supersymmetry transformations, action and algebraic structure. We show that the whole action, coupled to matter, can be written as the variation of a nilpotent operator, modulo field equations. An extended Slavnov-Taylor identity, collecting gauge symmetry and supersymmetry, is written, which allows to define the web of algebraic constraints, in view of the algebraic renormalization and of the extension of the non-renormalization theorems holding for N=2 SYM theory without matter.Comment: 28 pages, final version to be published on CQ

Study of the Gribov region in Euclidean Yang-Mills theories in the maximal Abelian gauge

The properties of the Gribov region in SU(2) Euclidean Yang-Mills theories in the maximal Abelian gauge are investigated. This region turns out to be bounded in all off-diagonal directions, while it is unbounded along the diagonal one. The soft breaking of the BRST invariance due to the restriction of the domain of integration in the path integral to the Gribov region is scrutinized. Owing to the unboundedness in the diagonal direction, the invariance with respect to Abelian transformations is preserved, a property which is at the origin of the local U(1) Ward identity of the maximal Abelian gauge.Comment: 15 pages, one reference added, version accepted for publication in Phys. Rev.