High-pressure batch reverse osmosis (RO) for zero liquid discharge (ZLD) in a Cr(III) electroplating process

A batch RO system was designed and built for high-pressure (120 bar) operation. The system was developed for a ZLD application involving treatment of metal plating wastewater from a Cr(III) electroplating process at a major industrial plant. Hybrid semi-batch/batch operation enabled a compact design to be achieved. To maximize water recovery without exceeding a set peak pressure, a method for controlling the switch point between semi-batch and batch phases was developed. The system was tested with feed representative of rinse water from the electroplating process. A range of feed concentrations (at 10–20× dilution of the plating bath), feed flows (0.21–0.46 m3/h), water fluxes (6–14 LMH) and water recoveries (87–95.7 %) were investigated. The system successfully recovered Cr(III) and restored its concentration to that of the electrolyte bath, thus meeting the requirements for reuse in the electroplating process. Rejection of most species was >99.8 %, sufficient for reuse of the permeate as rinse. However, rejection of boric acid was only 69–80 % such that a second RO pass may be needed to remove boric acid. Specific Energy Consumption was <2.25 kWh per m3 of treated rinse water, representing a 50-fold saving compared to the current method of treatment and disposal at the industrial plant

Structural diversity in the type IV pili of multidrug-resistant Acinetobacter

Acinetobacter baumannii is a Gram-negative coccobacillus found primarily in hospital settings that has recently emerged as a source of hospital-acquired infections. A. baumannii expresses a variety of virulence factors, including type IV pili, bacterial extracellular appendages often essential for attachment to host cells. Here, we report the high resolution structures of the major pilin subunit, PilA, from three Acinetobacter strains, demonstrating thatA. baumannii subsets produce morphologically distinct type IV pilin glycoproteins. We examine the consequences of this heterogeneity for protein folding and assembly as well as host-cell adhesion by Acinetobacter. Comparisons of genomic and structural data with pilin proteins from other species of soil gammaproteobacteria suggest that these structural differences stem from evolutionary pressure that has resulted in three distinct classes of type IVa pilins, each found in multiple species

Submillimeter Observations of The Isolated Massive Dense Clump IRAS 20126+4104

We used the CSO 10.4 meter telescope to image the 350 micron and 450 micron continuum and CO J=6-5 line emission of the IRAS 20126+4104 clump. The continuum and line observations show that the clump is isolated over a 4 pc region and has a radius of ~ 0.5 pc. Our analysis shows that the clump has a radial density profile propto r ^{-1.2} for r <~ 0.1 pc and has propto r^{-2.3} for r >~ 0.1 pc which suggests the inner region is infalling, while the infall wave has not yet reached the outer region. Assuming temperature gradient of r^{-0.35}, the power law indices become propto r ^{-0.9} for r < ~0.1 pc and propto r^{-2.0} for r >~ 0.1 pc. Based on a map of the flux ratio of 350micron/450micron, we identify three distinct regions: a bipolar feature that coincides with the large scale CO bipolar outflow; a cocoon-like region that encases the bipolar feature and has a warm surface; and a cold layer outside of the cocoon region. The complex patterns of the flux ratio map indicates that the clump is no longer uniform in terms of temperature as well as dust properties. The CO emission near the systemic velocity traces the dense clump and the outer layer of the clump shows narrow line widths (< ~3 km/s). The clump has a velocity gradient of ~ 2 km/s pc^{-1}, which we interpret as due to rotation of the clump, as the equilibrium mass (~ 200 Msun) is comparable to the LTE mass obtained from the CO line. Over a scale of ~ 1 pc, the clump rotates in the opposite sense with respect to the >~ 0.03 pc disk associated with the (proto)star. This is one of four objects in high-mass and low-mass star forming regions for which a discrepancy between the rotation sense of the envelope and the core has been found, suggesting that such a complex kinematics may not be unusual in star forming regions.Comment: 34 pages, 13 figures, Accepted for publication in the Ap

Generalized eigenfunctions and spectral theory for strongly local Dirichlet forms

We present an introduction to the framework of strongly local Dirichlet forms and discuss connections between the existence of certain generalized eigenfunctions and spectral properties within this framework. The range of applications is illustrated by a list of examples

Herpesvirus Telomerase RNA (vTR) with a Mutated Template Sequence Abrogates Herpesvirus-Induced Lymphomagenesis

Telomerase reverse transcriptase (TERT) and telomerase RNA (TR) represent the enzymatically active components of telomerase. In the complex, TR provides the template for the addition of telomeric repeats to telomeres, a protective structure at the end of linear chromosomes. Human TR with a mutation in the template region has been previously shown to inhibit proliferation of cancer cells in vitro. In this report, we examined the effects of a mutation in the template of a virus encoded TR (vTR) on herpesvirus-induced tumorigenesis in vivo. For this purpose, we used the oncogenic avian herpesvirus Marek's disease virus (MDV) as a natural virus-host model for lymphomagenesis. We generated recombinant MDV in which the vTR template sequence was mutated from AATCCCAATC to ATATATATAT (vAU5) by two-step Red-mediated mutagenesis. Recombinant viruses harboring the template mutation replicated with kinetics comparable to parental and revertant viruses in vitro. However, mutation of the vTR template sequence completely abrogated virus-induced tumor formation in vivo, although the virus was able to undergo low-level lytic replication. To confirm that the absence of tumors was dependent on the presence of mutant vTR in the telomerase complex, a second mutation was introduced in vAU5 that targeted the P6.1 stem loop, a conserved region essential for vTR-TERT interaction. Absence of vTR-AU5 from the telomerase complex restored virus-induced lymphoma formation. To test if the attenuated vAU5 could be used as an effective vaccine against MDV, we performed vaccination-challenge studies and determined that vaccination with vAU5 completely protected chickens from lethal challenge with highly virulent MDV. Taken together, our results demonstrate 1) that mutation of the vTR template sequence can completely abrogate virus-induced tumorigenesis, likely by the inhibition of cancer cell proliferation, and 2) that this strategy could be used to generate novel vaccine candidates against virus-induced lymphoma