In vitro gastrointestinal digestion of pea protein isolate as a function of pH, food matrices, autoclaving, high-pressure and re-heat treatments

This study investigated the influence of pH and processing conditions (autoclave at 93 °C/13 min or high pressure processing (HPP) at 600 MPa/5 min without/with follow-up reheating at 80 °C/30 min) on the digestibility of pea protein isolate. Both aqueous solutions and real food matrices (apple and carrot purees) containing pea protein was examined at 37 °C. In vitro gastrointestinal digestion was followed using sodium dodecyl sulphate polyacrylamide gel electrophoresis, titrimetric techniques and theoretical calculations. Pea protein with HPP followed by re-heating showed the highest rate of proteolysis in gastric conditions. In case of sequential intestinal digestion of the gastric chyme, pea protein at pH 6.2 demonstrated higher degree and rate of digestibility as compared to that at pH 3.6, the latter being close to the isoelectric point of pea protein. However, autoclave treatments overshadowed such pH effects. Processing-induced enhancement in digestibility might be attributed to the unfolding of the globular pea protein subunits. Pea protein in the carrot puree was more digestible than in the apple puree, due to apple procyanidins binding to pea protein. These new findings might have important implications in designing the process parameters and selection of appropriate food matrices for delivering pea protein

Organic Nanostructured Host-Guest Materials Containing Three Dyes

Dyes inserted into the parallel nanochannels of a fully organic crystal show efficient light conversion over a wide visible spectral region, with 100% conversion from UV to blue light. The Figure shows crystals containing either two (bottom) or three (top) dyes under U v illumination, and a top view of the structure of the dyes inserted into nanochannels

Quantifying bed‐related suspended load in gravel bed rivers through an analysis of the bedload‐suspended load relationship

International audienceSuspended load transport can strongly impact ecosystems, dam filling and water resources. However, contrary to bedload, the use of physically based predicting equations is very challenging because of the complexity of interactions between suspended load and the river system. Through the analysis of extensive data sets, we investigated extent to which one or several river bed or flow parameters could be used as a proxy for quantifying suspended fluxes in gravel bed rivers. For this purpose, we gathered in the literature nearly 2400 instantaneous field measurements collected in 56 gravel bed rivers. Among all standard dimensionless parameters tested, the strongest correlation was observed between the suspended sediment concentration and the dimensionless bedload rate. An empirical relation between these two parameters was calibrated. Used with a reach average bedload transport formula, the approach allowed to successfully reproduce suspended fluxes measured during major flood events in seven gravel bed alpine rivers, morphodynamically active and distant from hillslope sources. These results are discussed in light of the complexity of the processes potentially influencing suspended load in a mountainous context. The approach proposed in this paper will never replace direct field measurements, which can be considered the only confident method to assess sediment fluxes in alpine streams; however, it can increment existing panel tools that help river managers to estimate even rough but not unrealistic suspended fluxes when measurements are totally absent