19 research outputs found

    Stress-Induced Behavior: Chemotherapy without Drugs

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    Betel Chewing and Arecoline Affects Eotaxin-1, Asthma and Lung Function

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    Background: Betel nut is commonly used in many countries. Despite evidence suggesting an association with asthma, few studies have investigated the connection between betel nut use and asthma; thus, the underlying mechanism for the association with asthma is also unclear. the aim of this study was to investigate the association between betel chewing and asthma as well as the associations of plasma arecoline (a biomarker for exposure) and eotaxin-1 (a potential mediator) with asthma and lung function.Methods: We recruited 600 hospital-based asthmatic patients and 1200 age- and gender-matched community controls in southern Taiwan. To clarify the mechanism of action for eotaxin-1 in the association between betel chewing and asthma, we also designed an in vitro experiment to study the functional associations between arecoline exposure and eotaxin-1 levels.Results: A significant association was found between asthma and current betel chewing (adjusted odds ratio 2.05, 95% CI = 1.12-3.76), which was independent of potential confounders but was attenuated following adjustment for eotaxin-1. Arecoline and eotaxin-1 levels were positively correlated (Spearman r = 0.303, p = 0.02), while arecoline and arecaidine were negatively correlated with lung function. Functionally, arecoline alone does not induce eotaxin-1 release in vitro from dermal and gingival fibroblasts. However, in the presence of IL-4 and TNF-alpha, arecoline at 100 mg/ml induced more eotaxin-1 release than arecoline at 0 mu g/ml (2700 +/- 98 pg/ml vs 1850 +/- 142 pg/ml, p = 0.01 in dermal fibroblast cells, and 1489 +/- 78 pg/ml vs 1044 +/- 95 pg/ml, p = 0.03 in gingival fibroblast cells, respectively).Conclusion: Betel chewing is associated with asthma in this population, with arecoline induction of eotaxin-1 supported as a plausible causal pathway.National Health Research InstitutesNational Science Council, TaiwanNational Institute for Health Research (NIHR) Biomedical Research Centre and Dementia Biomedical Research Unit at South London and Maudsley NHS Foundation TrustKing's College LondonKaohsiung Med Univ, Coll Hlth Sci, Dept Publ Hlth, Kaohsiung, TaiwanKaohsiung Med Univ, Ctr Excellence Environm Med, Kaohsiung, TaiwanKaohsiung Med Univ, Kaohsiung Med Univ Hosp, Coll Med, Dept Internal Med,Div Pulm & Crit Care Med & Geri, Kaohsiung, TaiwanChang Gung Univ, Chang Gung Mem Hospital, Coll Med, Med Ctr,Dept Med,Div Pulm & Critical Care Med, Kaohsiung, TaiwanKaohsiung Med Univ, Dept Med & Appl Chem, Kaohsiung, TaiwanKaohsiung Chang Gung Mem Hosp, Dept Dermatol, Kaohsiung, TaiwanChang Gung Univ, Coll Med, Kaohsiung, TaiwanKaohsiung Med Univ, Coll Dent Med, Sch Dent, Kaohsiung, TaiwanChina Med Univ Hosp, Environment Omics Dis Res Ctr, Taichung, TaiwanChina Med Univ, Grad Inst Clin Med Sci, Taichung, TaiwanKaohsiung Med Univ, Kaohsiung Med Univ Hosp, Coll Med, Fac Med,Dept Internal Med,Div Pulm & Crit Care Me, Kaohsiung, TaiwanChung Shan Med Univ, Chung Shan Med Univ Hosp, Sch Med, Inst Med,Dept Psychiat, Taichung, TaiwanUniversidade Federal de São Paulo, Dept Psychobiol, São Paulo, BrazilKings Coll London, Inst Psychiat, London, EnglandUniversidade Federal de São Paulo, Dept Psychobiol, São Paulo, BrazilNational Health Research Institutes: NHRI-CN-PD9611PNational Science Council, Taiwan: NSC96-2314-B-037-040-MY3National Science Council, Taiwan: NSC100-2314-B-037-025-MY3Web of Scienc
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