15 new variable stars in Harvard Maps, Nos. 15, 18 and 27

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New Variable in Caelum.

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The Spectrum and Energy Levels of the Low-lying Configurations of Nd III

Emission spectra of neodymium (Nd, Z=60) were recorded using Penning and hollow cathode discharge lamps in the region 11500-54000 cm$^{-1}$ (8695-1852 \r{A}) by Fourier transform spectroscopy at resolving powers up to 106. Wavenumber measurements were accurate to a few 10$^{-3}$ cm$^{-1}$. Grating spectroscopy of Nd vacuum sliding sparks and stellar spectra were used to aid line and energy level identification. The classification of 433 transitions of doubly-ionised neodymium (Nd III) from the Penning lamp spectra resulted in the determination of 144 energy levels of the 4f$^4$, 4f$^3$5d, 4f$^3$6s, and 4f$^3$6p configurations of Nd III, 105 of which were experimentally established for the first time. Of the 40 previously published Nd III levels, 1 was revised and 39 were confirmed. New Nd III atomic structure calculations were made using the Cowan code parameterised by newly established levels. These results will not only benchmark and improve future semi-empirical atomic structure calculations of Nd III, but also enable more reliable astrophysical applications of Nd III, such as abundance analyses of kilonovae and chemically peculiar stars, and studies of pulsational wave propagation in these stars

A novel assay for monitoring internalization of nanocarrier coupled antibodies

BACKGROUND: Discovery of tumor-selective antibodies or antibody fragments is a promising approach for delivering therapeutic agents to antigen over-expressing cancers. Therefore it is important to develop methods for the identification of target- and function specific antibodies for effective drug delivery. Here we describe a highly selective and sensitive method for characterizing the internalizing potential of multivalently displayed antibodies or ligands conjugated to liposomes into tumor cells. The assay requires minute amounts of histidine-tagged ligand and relies on the non-covalent coupling of these antibodies to fluorescent liposomes containing a metal ion-chelating lipid. Following incubation of cells with antibody-conjugated liposomes, surface bound liposomes are gently removed and the remaining internalized liposomes are quantitated based on fluorescence in a high throughput manner. We have termed this methodology "Chelated Ligand Internalization Assay", or CLIA. RESULTS: The specificity of the assay was demonstrated with different antibodies to the ErbB-2 and EGF receptors. Antibody-uptake correlated with receptor expression levels in tumor cell lines with a range of receptor expression. Furthermore, Ni-NTA liposomes containing doxorubicin were used to screen for the ability of antibodies to confer target-specific cytotoxicity. Using an anti-ErbB2 single chain Fv (scFv) (F5) antibody, cytotoxicity could be conferred to ErbB2-overexpressing cells; however, a poly(ethylene glycol)-linked lipid (DSPE-PEG-NTA-Ni) was necessary to allow for efficient loading of the drug and to reduce nonspecific drug leakage during the course of the assay. CONCLUSION: The CLIA method we describe here represents a rapid, sensitive and robust assay for the identification and characterization of tumor-specific antibodies capable of high drug-delivery efficiency when conjugated to liposomal nanocarriers

The microstructure and hardness of Ni-Co-Al-Ti-Cr quinary alloys

The effects of Ni:Co and Al:Ti ratios on the room temperature microstructure, hardness and lattice parameter of twenty-seven quinary Ni-Co-Al-Ti-Cr alloys have been evaluated. All of the alloys exhibited a uniform $\gamma$-$\gamma ^\prime$ microstructure. Differential scanning calorimetry (DSC) showed that the liquidus and solidus temperatures of the alloys increased with greater Al:Ti ratios, decreased with Cr concentration and remained largely unchanged with respect to the Ni:Co ratio. Neutron diffraction measurements of the $\gamma$ and $\gamma ^\prime$ lattice parameters revealed that the lattice misfit in all of the alloys was positive and increased with Ti concentration (i.e. lower Al:Ti ratio) regardless of the concentration of Cr, or the ratio of Ni:Co. Importantly, alloys with a Ni:Co ratio of 1:1, were found to have consistently greater lattice misfits than alloys with Ni:Co ratios of either 1:3 or 3:1. The measured lattice misfits were found to be strongly correlated with the Vickers hardness of the alloys, suggesting that lattice misfit plays a key role in the strengthening of $\gamma$-$\gamma ^\prime$ alloys of this type.Rolls-Royce/EPSRC Strategic Partnership (Grant IDs: EP/H022309/1, EP/H500375/1 and EP/ M005607/1)This is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.jallcom.2016.07.15

A Far Ultraviolet Study of the Old Nova V841 Oph

We have carried out a synthetic spectral analysis of archival IUE spectra of the old nova V841 Oph (Nova Oph 1848) taken 15 years apart. The spectra reveal a rising continuum shortward of 1560\AA, a C {\sc iv} P-Cygni profile indicative of wind outflow associated with disk accretion in one spectrum, a deep Ly $\alpha$ profile, and strong N {\sc v} (1238\AA, 1242\AA) and O {\sc v} (1371\AA) wind/coronal absorption lines. Numerous sharp interstellar resonance lines are also present. A grid of high gravity atmospheres and accretion disk models, spanning a wide range of inclinations, accretion rates and white dwarf masses was compared to the de-reddened spectra. We find that, for a steady state accretion disk model to account for the FUV spectra, the accretion rate is only $\sim$3 $\times$ 10$^{-11}$ M_{\sun}/yr, 147 years after its outburst in 1848, with an implied distance within $\sim$300 pc. The accretion rate at 147 years post-outburst is smaller than expected for an old nova.Comment: 6 b/w figures, 2 tables, preprint accepted in the November 2005 issue of PAS

Report of the Astronomy Committee

The present report relates only to the scientific needs of Astronomy. Its applications to the possible services that astronomers can render in the war, as a part of the work done by the National Research Council in connection with the Council of National Defense, will be made the subject of a separate study

Therapeutic efficacy of anti-ErbB2 immunoliposomes targeted by a phage antibody selected for cellular endocytosis

AbstractMany targeted cancer therapies require endocytosis of the targeting molecule and delivery of the therapeutic agent to the interior of the tumor cell. To generate single chain Fv (scFv) antibodies capable of triggering receptor-mediated endocytosis, we previously developed a method to directly select phage antibodies for internalization by recovering infectious phage from the cytoplasm of the target cell. Using this methodology, we reported the selection of a panel of scFv that were internalized into breast cancer cells from a nonimmune phage library. For this work, an immunotherapeutic was generated from one of these scFv (F5), which bound to ErbB2 (HER2/neu). The F5 scFv was reengineered with a C-terminal cysteine, expressed at high levels in Escherichia coli, and coupled to sterically stabilized liposomes. F5 anti-ErbB2 immunoliposomes were immunoreactive as determined by surface plasmon resonance (SPR) and were avidly internalized by ErbB2-expressing tumor cell lines in proportion to the levels of ErbB2 expression. F5-scFv targeted liposomes containing doxorubicin had antitumor activity and produced significant reduction in tumor size in xenografted mice compared to nontargeted liposomes containing doxorubicin. This strategy should be applicable to generate immunotherapeutics for other malignancies by selecting phage antibodies for internalization into other tumor types and using the scFv to target liposomes or other nanoparticles

Acute kidney disease and renal recovery : consensus report of the Acute Disease Quality Initiative (ADQI) 16 Workgroup

Consensus definitions have been reached for both acute kidney injury (AKI) and chronic kidney disease (CKD) and these definitions are now routinely used in research and clinical practice. The KDIGO guideline defines AKI as an abrupt decrease in kidney function occurring over 7 days or less, whereas CKD is defined by the persistence of kidney disease for a period of > 90 days. AKI and CKD are increasingly recognized as related entities and in some instances probably represent a continuum of the disease process. For patients in whom pathophysiologic processes are ongoing, the term acute kidney disease (AKD) has been proposed to define the course of disease after AKI; however, definitions of AKD and strategies for the management of patients with AKD are not currently available. In this consensus statement, the Acute Disease Quality Initiative (ADQI) proposes definitions, staging criteria for AKD, and strategies for the management of affected patients. We also make recommendations for areas of future research, which aim to improve understanding of the underlying processes and improve outcomes for patients with AKD

Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD

BACKGROUND: A new locus for amyotrophic lateral sclerosis – frontotemporal dementia (ALS-FTD) has recently been ascribed to chromosome 9p. METHODS: We identified chromosome 9p segregating haplotypes within two families with ALS-FTD (F476 and F2) and undertook mutational screening of candidate genes within this locus. RESULTS: Candidate gene sequencing at this locus revealed the presence of a disease segregating stop mutation (Q342X) in the intraflagellar transport 74 (IFT74) gene in family 476 (F476), but no mutation was detected within IFT74 in family 2 (F2). While neither family was sufficiently informative to definitively implicate or exclude IFT74 mutations as a cause of chromosome 9-linked ALS-FTD, the nature of the mutation observed within F476 (predicted to truncate the protein by 258 amino acids) led us to sequence the open reading frame of this gene in a large number of ALS and FTD cases (n = 420). An additional sequence variant (G58D) was found in a case of sporadic semantic dementia. I55L sequence variants were found in three other unrelated affected individuals, but this was also found in a single individual among 800 Human Diversity Gene Panel samples. CONCLUSION: Confirmation of the pathogenicity of IFT74 sequence variants will require screening of other chromosome 9p-linked families