Stakeholder involvement in wastewater treatment design

The design option appraisal stage of new water industry capital investment projects involve greater levels of complexity than those generally encountered in other stages of the design process. It is at this stage that the issues related to sustainable development will be introduced, as decisions are multi-stakeholder and therefore decision criteria that reflect the views of a wide range of stakeholders with a range of different viewpoints need to be incorporated. This paper describes a case study which maps information flows, identifies decision criteria and evaluates the extent of stakeholder involvement in the design stage of a major UK wastewater system project. A mapping technique was developed to document and represent the flow of information during the decision making process. An in-depth study of the information flows enabled the researchers to establish the extent to which sustainability criteria were actually used. This was compared with recognised sustainability criteria identified in previous research involving the authors to allow an assessment of the effectiveness of stakeholder representation. Recommendations are given regarding ways of improving stakeholder involvement in water industry asset investment decisions

The effect of Me2_{2}SO overexposure during cryopreservation on HOS TE85 and hMSC viability, growth and quality

With the cell therapy industry continuing to grow, the ability to preserve clinical grade cells, including mesenchymal stem cells (MSCs), whilst retaining cell viability and function remains critical for the generation of off-the-shelf therapies. Cryopreservation of MSCs, using slow freezing, is an established process at lab scale. However, the cytotoxicity of cryoprotectants, like Me$_{2}$SO, raises questions about the impact of prolonged cell exposure to cryoprotectant at temperatures >0 °C during processing of large cell batches for allogenic therapies prior to rapid cooling in a controlled rate freezer or in the clinic prior to administration. Here we show that exposure of human bone marrow derived MSCs to Me$_{2}$SO for ≥1 h before freezing, or after thawing, degrades membrane integrity, short-term cell attachment efficiency and alters cell immunophenotype. After 2 h's exposure to Me$_{2}$SO at 37 °C post-thaw, membrane integrity dropped to ∼70% and only ∼50% of cells retained the ability to adhere to tissue culture plastic. Furthermore, only 70% of the recovered MSCs retained an immunophenotype consistent with the ISCT minimal criteria after exposure. We also saw a similar loss of membrane integrity and attachment efficiency after exposing osteoblast (HOS TE85) cells to Me$_{2}$SO before, and after, cryopreservation. Overall, these results show that freezing medium exposure is a critical determinant of product quality as process scale increases. Defining and reporting cell sensitivity to freezing medium exposure, both before and after cryopreservation, enables a fair judgement of how scalable a particular cryopreservation process can be, and consequently whether the therapy has commercial feasibility.The authors would like to acknowledge the Engineering and Physical Sciences Research Council (EPSRC; UK, EP/F500491/1) and Bioprocessing Research Industry Club (BBSRC/BRIC; UK, BB/I017602/1) for their support and funding

Quantum geometry from 2+1 AdS quantum gravity on the torus

Wilson observables for 2+1 quantum gravity with negative cosmological constant, when the spatial manifold is a torus, exhibit several novel features: signed area phases relate the observables assigned to homotopic loops, and their commutators describe loop intersections, with properties that are not yet fully understood. We describe progress in our study of this bracket, which can be interpreted as a q-deformed Goldman bracket, and provide a geometrical interpretation in terms of a quantum version of Pick's formula for the area of a polygon with integer vertices.Comment: 19 pages, 11 figures, revised with more explanations, improved figures and extra figures. To appear GER

Coherent control of addressable Rydberg atoms for hybrid quantum information processing

Neutral atoms provide an excellent resource for quantum information processing, combining the long atomic coherence times of the hyperfine ground-state with strong dipole-dipole interactions of highly excited Rydberg states for generating deterministic entanglement between qubits separated by < 10 µm [1]. Scalable long-range interactions can be obtained by coupling the atomic array to a superconducting microwave cavity enabling hybrid quantum information processing where the cavity-mediated entanglement allows atoms to be coupled over cm length scales. We present the first steps towards such an experiment incorporating high fidelity readout using an sCMOS camera [2] and the ability to drive fast, optically addressable rotations of the hyperfine-encoded qubits to the Rydberg state. Using our sub-kHz cavitystabilised laser system [3] we demonstrate coherent control of single Rydberg atoms, performing Ramsey spectroscopy to determine coherence time and to generate entanglement between a pair of atoms separated by 6 µm. Combining this excitation scheme with our ground-state Raman lasers we show progress towards the implementation of a mesoscopic Rydberg gate based on electromagnetically induced transparency (EIT) offering robust entanglement of multi-atom ensembles [4]

Entanglement of neutral-atom qubits with long ground-Rydberg coherence times

We report results of a ground-state entanglement protocol for a pair of Cs atoms separated by 6~μm, combining the Rydberg blockade mechanism with a two-photon Raman transitions to prepare the |Ψ+⟩=(|10⟩+|01⟩)/2‾√ Bell state with a loss-corrected fidelity of 0.81(5), equal to the best demonstrated fidelity for atoms trapped in optical tweezers but without the requirement for dynamically adjustable interatomic spacing. Qubit state coherence is also critical for quantum information applications, and we characterise both ground-state and ground-Rydberg dephasing rates using Ramsey spectroscopy. We demonstrate transverse dephasing times T∗2=10(1)~ms and T′2=0.14(1)~s for the qubit levels and achieve long ground-Rydberg coherence times of T∗2=17(2) μs as required for implementing high-fidelity multi-qubit gate sequences where a control atom remains in the Rydberg state while applying local operations on neighbouring target qubits

Comparing oncologic outcomes in patients undergoing surgery for oncocytic neoplasms, conventional oncocytoma, and chromophobe renal cell carcinoma

Introduction Oncocytic neoplasms are renal tumors similar to oncocytoma, but their morphologic variations preclude definitive diagnosis. This somewhat confusing diagnosis can create treatment and surveillance challenges for the treating urologist. We hypothesize that these subtle morphologic variations do not drastically affect the malignant potential of these tumors, and we sought to demonstrate this by comparing clinical outcomes of oncocytic neoplasms to those of classic oncocytoma and chromophobe. Methods We gathered demographic and outcomes data for patients with variant oncocytic tumors. Oncologic surveillance was conducted per institutional protocol in accordance with NCCN guidelines. Descriptive statistics were used to compare incidence of metastasis and death against those for patients with oncocytoma and chromophobe. Three hundred and fifty-one patients were analyzed: 164 patients with oncocytoma, 28 with oncocytic neoplasms, and 159 with chromophobe tumors. Results Median follow-up time for the entire cohort was 32.4 months, (interquartile range 9.2–70.0). Seventeen total patients (17/351, 4.9%) died during the course of the study. In patients with oncocytoma or oncocytic neoplasm, none were known to metastasize or die of their disease. Only chromophobe tumors >6 cm in size in our series demonstrated metastatic progression and approximately half of these metastasized tumors demonstrated sarcomatoid changes. Conclusion Variant oncocytic neoplasms appear to have a natural course similar to classic oncocytoma. These tumors appear to have no metastatic potential, and oncologic surveillance may not be indicated after surgery