Reading Development in At-risk Kindergarten Students: A Tier 2 Response-to-intervention RTI Program Using Research-Based Principles in Early Literacy

Early identification of struggling readers and direct instruction for these readers are effective in the prevention and treatment of reading problems (Torgesen, 2002). The practice of wait-tofail is being challenged by the responsiveness to intervention (RTI) models, which promote early identification of at-risk students, progress monitoring, and implementation of researched-based tiered interventions. The prereading skills that have been identified as being necessary for future reading achievement include phonological awareness, letter identification, the alphabetic principle, orthography, and rapid automatized naming. The purpose of this CUl1\u27ent study is to examine the effectiveness of a Tier 2 intervention program that targets these essential pre reading skills with at-risk kindergarten students and to assess the effectiveness of this intervention. Students received either the fall only, winter only, or all year intervention, two times per week for one-on-one instruction, with progress monitoring occurring at mid-points. Results suggest that a Tier 2 intervention program can significantly improve critical prereading skills with at-risk students and that these improvements can be sustained at the beginning of first grade. Project K groups were able to positively change their reading trajectories and most were not significantly different from the typical mean performance, with no groups falling below the some-risk benchmark, at post-test. Slow responders required more time to learn and to transfer critical prereading skills but with persistent intervention, significant progress was made. Strong responders to the fall intervention benefitted significantly from instruction, which produced high inoculation effects during kindergarten in all preliteracy skills. The different response rates of students are worthy of educators\u27 attention before detelmining whether or not a student should be considered as a nomesponder. As educators and psychologists begin to implement the RTI model within schools, several aspects need to be addressed through research to ensure consistency and to avoid some of the same criticisms of the discrepancy model. Some areas that need to be defined include the elements that constitute a nomesponder, ways to assess a nomesponder, and minimal length of time required of a Tier 2 intervention model

The hormone response element mimic sequence of GAS5 lncRNA is sufficient to induce apoptosis in breast cancer cells.

Growth arrest-specific 5 (GAS5) lncRNA promotes apoptosis, and its expression is down-regulated in breast cancer. GAS5 lncRNA is a decoy of glucocorticoid/related receptors; a stem-loop sequence constitutes the GAS5 hormone response element mimic (HREM), which is essential for the regulation of breast cancer cell apoptosis. This preclinical study aimed to determine if the GAS5 HREM sequence alone promotes the apoptosis of breast cancer cells. Nucleofection of hormone-sensitive and -insensitive breast cancer cell lines with a GAS5 HREM DNA oligonucleotide increased both basal and ultraviolet-C-induced apoptosis, and decreased culture viability and clonogenic growth, similar to GAS5 lncRNA. The HREM oligonucleotide demonstrated similar sequence specificity to the native HREM for its functional activity and had no effect on endogenous GAS5 lncRNA levels. Certain chemically modified HREM oligonucleotides, notably DNA and RNA phosphorothioates, retained pro-apoptotic. activity. Crucially the HREM oligonucleotide could overcome apoptosis resistance secondary to deficient endogenous GAS5 lncRNA levels. Thus, the GAS5 lncRNA HREM sequence alone is sufficient to induce apoptosis in breast cancer cells, including triple-negative breast cancer cells. These findings further suggest that emerging knowledge of structure/function relationships in the field of lncRNA biology can be exploited for the development of entirely novel, oligonucleotide mimic-based, cancer therapies.Breast Cancer No

Targeting long non-coding RNAs (lncRNAs) with oligonucleotides in cancer therapy

This document is the Accepted Manuscript version of a published work that appeared in final form in Translational Cancer Research. To access the final edited and published work see http://dx.doi.org/10.21037/tcr.2016.10.63No abstrac

Low attentional engagement makes attention network activity susceptible to emotional interference

The aim of this study was to investigate whether emotion-attention interaction depends on attentional engagement. To investigate emotional modulation of attention network activation, we used a functional MRI paradigm consisting of a visuospatial attention task with either frequent (high-engagement) or infrequent (low-engagement) targets and intermittent emotional or neutral distractors. The attention task recruited a bilateral frontoparietal network with no emotional interference on network activation when the attentional engagement was high. In contrast, when the attentional engagement was low, the unpleasant stimuli interfered with the activation of the frontoparietal attention network, especially in the right hemisphere. This study provides novel evidence for low attentional engagement making attention control network activation susceptible to emotional interference. © 2014 Wolters Kluwer Health | Lippincott Williams & Wilkins.Fil: Exposito, Veronica. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Tampere; FinlandiaFil: Pickard, Natasha. California State University; Estados UnidosFil: Solbakk, Anne-Kristin. University of Oslo; NoruegaFil: Ogawa, Keith H.. Saint Mary's College Of California; Estados UnidosFil: Knight, Robert T.. California State University; Estados UnidosFil: Hartikainen, Kaisa M.. Universidad de Tampere; Finlandi

Comparing word2vec and GloVe for Automatic Measurement of MWE Compositionality

This paper explores the use of word2vec and GloVe embeddings for unsupervised measurement of the semantic compositionality of MWE candidates. Through comparison with several human-annotated reference sets, we find word2vec to be substantively superior to GloVe for this task. We also find Simple English Wikipedia to be a poor-quality resource for compositionality assessment, but demonstrate that a sample of 10% of sentences in the English Wikipedia can provide a conveniently tractable corpus with only moderate reduction in the quality of outputs

GAS5 lncRNA Modulates the Action of mTOR Inhibitors in Prostate Cancer Cells

Background There is a need to develop new therapies for castrate-resistant prostate cancer (CRPC) and growth arrest-specific 5 (GAS5) long non-coding RNA (lncRNA), which riborepresses androgen receptor action, may offer novel opportunities in this regard. GAS5 lncRNA expression declines as prostate cancer cells acquire castrate-resistance, and decreased GAS5 expression attenuates the responses of prostate cancer cells to apoptotic stimuli. Enhancing GAS5 lncRNA expression may therefore offer a strategy to improve the effectiveness of chemotherapeutic agents. GAS5 is a member of the 5' terminal oligopyrimidine gene family, and we have therefore examined if mTOR inhibition can enhance cellular GAS5 levels in prostate cancer cells. In addition, we have determined if GAS5 lncRNA itself is required for mTOR inhibitor action in prostate cancer cells, as recently demonstrated in lymphoid cells. Method The effects of mTOR inhibitors on GAS5 lncRNA expression and cell proliferation were determined in a range of prostate cancer cell lines. Transfection of cells with GAS5 siRNA and plasmid constructs was performed to determine the involvement of GAS5 lncRNA in mTOR inhibitor action. Results Treatment with rapamycin and rapalogues increased cellular GAS5 levels and inhibited culture growth in both androgen-dependent (LNCaP) and androgen-sensitive (22Rv1) cell lines, but not in androgen-independent (PC-3 and DU145) cells. GAS5 silencing in both LNCaP and 22Rv1 cells decreased their sensitivity to growth inhibition by mTOR inhibitors. Moreover, transfection of GAS5 lncRNA sensitized PC-3 and DU145 cells to mTOR inhibitors, resulting in inhibition of culture growth. Conclusion mTOR inhibition enhances GAS5 transcript levels in some, but not all, prostate cancer cell lines. This may in part be related to endogenous levels of GAS5 expression, which tend to be lower in prostate cancer cells representative of advanced disease, particularly since current findings demonstrate a role for GAS5 lncRNA in mTOR inhibitor action in prostate cancer cells