The Effects of Cued Speech on Phonemic Awareness Skills

Research suggests phonemic awareness is enhanced through multimodality training. Cued Speech is a multimodality system that combines hand signs with mouth movements to represent phonemes of the spoken language. This system has been utilized successfully in developing phonological awareness with children with hearing loss. However, no research is available on its effectiveness with children who are not deaf or hard-of-hearing. The efficacy of the use of Cued Speech for the enhancement of phonological skills in typically developing 1st grade students was evaluated in this study. Twenty-six 1st graders identified as low-achieving readers by their classroom teachers were administered the PPVT-4 to match participants across three assigned research groups: no intervention (NI), phonemic awareness training auditory only (AO), or phonemic awareness training with Cued Speech (CS). Pre- and post-test scores were compared on six different skills from the Phonological Awareness Test 2 (PAT-2). Results indicated that the Cued Speech intervention group made more gains based on Phonological Awareness Test 2 pre- and post-intervention scores. Although no statistical significance was found when all three groups\u27 post-intervention scores were compared, the CS group did show significant gains across its participants

The Effects of Cued Speech on Phonemic Awareness Skills

Research suggests phonemic awareness is enhanced through multimodality training. Cued Speech is a multimodality system that combines hand signs with mouth movements to represent phonemes of the spoken language. This system has been utilized successfully in developing phonological awareness with children with hearing loss. However, no research is available on its effectiveness with children who are not deaf or hard-of-hearing. The efficacy of the use of Cued Speech for the enhancement of phonological skills in typically developing 1st grade students was evaluated in this study. Twenty-six 1st graders identified as low-achieving readers by their classroom teachers were administered the PPVT-4 to match participants across three assigned research groups: no intervention (NI), phonemic awareness training auditory only (AO), or phonemic awareness training with Cued Speech (CS). Pre- and post-test scores were compared on six different skills from the Phonological Awareness Test 2 (PAT-2). Results indicated that the Cued Speech intervention group made more gains based on Phonological Awareness Test 2 pre- and post-intervention scores. Although no statistical significance was found when all three groups\u27 post-intervention scores were compared, the CS group did show significant gains across its participants

Visualizing Sensor Network Coverage with Location Uncertainty

We present an interactive visualization system for exploring the coverage in sensor networks with uncertain sensor locations. We consider a simple case of uncertainty where the location of each sensor is confined to a discrete number of points sampled uniformly at random from a region with a fixed radius. Employing techniques from topological data analysis, we model and visualize network coverage by quantifying the uncertainty defined on its simplicial complex representations. We demonstrate the capabilities and effectiveness of our tool via the exploration of randomly distributed sensor networks

The Impact of Low Retention of Nonprofit Organizations

This study explored the impact of low retention rates in nonprofit organizations and review factors that are associated with this low rate. Nonprofit organizations are a crucial and integral part of our community, there are many factors that are influential in the retention of employees which impact the service that is available to those in need. The need for this study emerged from the researcher’s desire to raise awareness of the factors that lead to low retention rates in social service non-profit organizations and its effects on service provision This study allowed for administration to implement strategies that address the factors that lead to high turnover. Prevention strategies that can be utilized to decrease the dissatisfaction of current employees and increase engagement. The field of social services has been chosen for study because its programs are intended to improve the basic social welfare status of individual lives such that society is reformed. The following subsections were discussed: Growth of nonprofit organizations, nonprofits and human need, nonprofit relationship with funders, challenges within Nonprofits, why employees depart from these organizations and, retention focus. The research method used for this study was quantitative data. Since the research method used for this study was the methodology of quantitative design, snowball sampling was utilized. The Quantitative design was chosen to collect data from a larger group of service providers who worked primarily in a not for profit setting through Likert Scale surveys. The design assisted in quantifying the answer from the surveys to allow comparison, recognition of discrepancies, and or correlations of the iv data. The data was collected from 33 participants through an online survey program, utilizing Survey Monkey and, results were entered in SPSS, to compare relationships. Independent variable were the factors that contribute to low retention rate, and the dependent variable was the retention rate. The population this study focused on was management, staff and, direct service employees who work in social service nonprofits or have worked in the past. This study shows statistics which include ethnicity, age of the participants, marital status, education, and employment status. Along with statistical information on each item presented in the distributed survey, it outlines the statistical analysis that was conducted which includes a correlation study on the relationship between compensation, job training, professional development, filled positions, funding, disruption in services, self-care, access to management, and staff appreciate, and staff’s desire to leave a non-profit organization. The data was analyzed and found that the biggest impact to employee retention is compensation and the stress caused by chronically vacant positions. The study reviews the conclusions relative to our research questions and hypothesis in addition to, theories guiding conceptualization. This study discusses the limitations of study design and, procedures, as well as recommendations for future social work practice and policy. The findings were significant because the study could narrow down two out of the five factors that demonstrated an actual impact on retention in non-profit social service organizations, which gives non-profit managements clear barriers that they can attempt to address

Vortex fluidics-mediated DNA rescue from formalin-fixed museum specimens.

DNA from formalin-preserved tissue could unlock a vast repository of genetic information stored in museums worldwide. However, formaldehyde crosslinks proteins and DNA, and prevents ready amplification and DNA sequencing. Formaldehyde acylation also fragments the DNA. Treatment with proteinase K proteolyzes crosslinked proteins to rescue the DNA, though the process is quite slow. To reduce processing time and improve rescue efficiency, we applied the mechanical energy of a vortex fluidic device (VFD) to drive the catalytic activity of proteinase K and recover DNA from American lobster tissue (Homarus americanus) fixed in 3.7% formalin for >1-year. A scan of VFD rotational speeds identified the optimal rotational speed for recovery of PCR-amplifiable DNA and while 500+ base pairs were sequenced, shorter read lengths were more consistently obtained. This VFD-based method also effectively recovered DNA from formalin-preserved samples. The results provide a roadmap for exploring DNA from millions of historical and even extinct species

NASA Advanced Concepts Office, Earth-To-Orbit Team Design Process and Tools

The Earth-to-Orbit Team (ETO) of the Advanced Concepts Office (ACO) at NASA Marshall Space Flight Center (MSFC) is considered the pre-eminent go-to group for pre-phase A and phase A concept definition. Over the past several years the ETO team has evaluated thousands of launch vehicle concept variations for a significant number of studies including agency-wide efforts such as the Exploration Systems Architecture Study (ESAS), Constellation, Heavy Lift Launch Vehicle (HLLV), Augustine Report, Heavy Lift Propulsion Technology (HLPT), Human Exploration Framework Team (HEFT), and Space Launch System (SLS). The ACO ETO Team is called upon to address many needs in NASA s design community; some of these are defining extremely large trade-spaces, evaluating advanced technology concepts which have not been addressed by a large majority of the aerospace community, and the rapid turn-around of highly time critical actions. It is the time critical actions, those often limited by schedule or little advanced warning, that have forced the five member ETO team to develop a design process robust enough to handle their current output level in order to meet their customer s needs. Based on the number of vehicle concepts evaluated over the past year this output level averages to four completed vehicle concepts per day. Each of these completed vehicle concepts includes a full mass breakdown of the vehicle to a tertiary level of subsystem components and a vehicle trajectory analysis to determine optimized payload delivery to specified orbital parameters, flight environments, and delta v capability. A structural analysis of the vehicle to determine flight loads based on the trajectory output, material properties, and geometry of the concept is also performed. Due to working in this fast-paced and sometimes rapidly changing environment, the ETO Team has developed a finely tuned process to maximize their delivery capabilities. The objective of this paper is to describe the interfaces between the three disciplines used in the design process: weights and sizing, trajectory, and structural analysis. The tools used to perform such analysis are INtegrated Rocket Sizing (INTROS), Program to Optimize Simulated Trajectories (POST), and Launch Vehicle Analysis (LVA) respectively. The methods each discipline uses to streamline their particular part of the design process will also be discussed

Adenomatoid tumors of the male and female genital tract are defined by TRAF7 mutations that drive aberrant NF-kB pathway activation.

Adenomatoid tumors are the most common neoplasm of the epididymis, and histologically similar adenomatoid tumors also commonly arise in the uterus and fallopian tube. To investigate the molecular pathogenesis of these tumors, we performed genomic profiling on a cohort of 31 adenomatoid tumors of the male and female genital tracts. We identified that all tumors harbored somatic missense mutations in the TRAF7 gene, which encodes an E3 ubiquitin ligase belonging to the family of tumor necrosis factor receptor-associated factors (TRAFs). These mutations all clustered into one of five recurrent hotspots within the WD40 repeat domains at the C-terminus of the protein. Functional studies in vitro revealed that expression of mutant but not wild-type TRAF7 led to increased phosphorylation of nuclear factor-kappa B (NF-kB) and increased expression of L1 cell adhesion molecule (L1CAM), a marker of NF-kB pathway activation. Immunohistochemistry demonstrated robust L1CAM expression in adenomatoid tumors that was absent in normal mesothelial cells, malignant peritoneal mesotheliomas and multilocular peritoneal inclusion cysts. Together, these studies demonstrate that adenomatoid tumors of the male and female genital tract are genetically defined by TRAF7 mutation that drives aberrant NF-kB pathway activation

Missense-depleted regions in population exomes implicate ras superfamily nucleotide-binding protein alteration in patients with brain malformation.

Genomic sequence interpretation can miss clinically relevant missense variants for several reasons. Rare missense variants are numerous in the exome and difficult to prioritise. Affected genes may also not have existing disease association. To improve variant prioritisation, we leverage population exome data to identify intragenic missense-depleted regions (MDRs) genome-wide that may be important in disease. We then use missense depletion analyses to help prioritise undiagnosed disease exome variants. We demonstrate application of this strategy to identify a novel gene association for human brain malformation. We identified de novo missense variants that affect the GDP/GTP-binding site of ARF1 in three unrelated patients. Corresponding functional analysis suggests ARF1 GDP/GTP-activation is affected by the specific missense mutations associated with heterotopia. These findings expand the genetic pathway underpinning neurologic disease that classically includes FLNA. ARF1 along with ARFGEF2 add further evidence implicating ARF/GEFs in the brain. Using functional ontology, top MDR-containing genes were highly enriched for nucleotide-binding function, suggesting these may be candidates for human disease. Routine consideration of MDR in the interpretation of exome data for rare diseases may help identify strong genetic factors for many severe conditions, infertility/reduction in reproductive capability, and embryonic conditions contributing to preterm loss

Optimising Antimicrobial Selection and Duration in the Treatment of Febrile Neutropenia in Children

Febrile neutropenia (FN) is a frequent complication of cancer treatment in children. Owing to the potential for overwhelming bacterial sepsis, the recognition and management of FN requires rapid implementation of evidenced-based management protocols. Treatment paradigms have progressed from hospitalisation with broad spectrum antibiotics for all patients, through to risk adapted approaches to management. Such risk adapted approaches aim to provide safe care through incorporating antimicrobial stewardship (AMS) principles such as implementation of comprehensive clinical pathways incorporating de-escalation strategies with the imperative to reduce hospital stay and antibiotic exposure where possible in order to improve patient experience, reduce costs and diminish the risk of nosocomial infection. This review summarises the principles of risk stratification in FN, the current key considerations for optimising empiric antimicrobial selection including knowledge of antimicrobial resistance patterns and emerging technologies for rapid diagnosis of specific infections and summarises existing evidence on time to treatment, investigations required and duration of treatment. To aid treating physicians we suggest the key features based on current evidence that should be part of any FN management guideline and highlight areas for future research. The focus is on treatment of bacterial infections although fungal and viral infections are also important in this patient group

Infant behavioral reactivity predicts change in amygdala volume 12 years later

The current study examined the link between temperamental reactivity in infancy and amygdala development in middle childhood. A sample (n = 291) of four-month-old infants was assessed for infant temperament, and two groups were identified: those exhibiting negative reactivity (n = 116) and those exhibiting positive reactivity (n = 106). At 10 and 12 years of age structural imaging was completed on a subset of these participants (n = 75). Results indicate that, between 10 and 12 years of age, left amygdala volume increased more slowly in those with negative compared to positive reactive temperament. These results provide novel evidence linking early temperament to distinct patterns of brain development over middle childhood