Aggressive Interactions of Rocky Mountain Elk, Cervus elaphus nelsoni, During the Calving Season Toward Mule Deer, Odocoileus hemionus, in Central Colorado

We documented four aggressive interactions between Rocky Mountain Elk (Cervus elaphus) and Mule Deer (Odocoileus hemionus) during the Elk calving season of June and July 1995. In one case, we believe a fawn Mule Deer was killed by two cow Elk. In the other three cases, Elk chased Mule Deer away from an area where they were grazing. These incidents are of interest because documentation of such interactions between Elk and Mule Deer is sparse in the scientific literature and because of the concern about declining Mule Deer populations throughout the western United States

The Effectiveness of “Emolabeling” to Promote Healthy Food Choices in Children Preschool Through 5th Grade

Obesity has become a growing global concern. Evidence indicates that ecological factors are most predictive of obesity among children, and that a new strategy, referred to as emolabeling, may effectively address ecological factors, although the extent to which it can influence food choice is not yet known, but tested here. Specifically, we tested the hypothesis that children aged 3 to 11 years will use emolabels, or emotional correlates of health (i.e. healthy-happy, unhealthy-sad), to make healthy food choices. A cross-sectional design was used with two phases. In Phase 1, children were taught how to use emolabels with a “faces of health” lesson. In Phase 2, children made choices between containers that were laid out on a large table in pairs and varied by taste (tastes good, no information), social norms (popular, not popular), branding (image of a minion, no image), or preference (told what food was in each container). A control pair was labeled with only emoticons. The order and presentation of the containers were counterbalanced for each variation. Results showed that a significant proportion of children in the pre-literacy and the early literacy grades used emoticons to specifically make healthy food choices in each variation (p < .05 for all tests), except when children were told what foods were in the containers. In all, emolabeling effectively influenced food choices for healthy foods among children aged 3 to 11 years, more so than labeling for taste, social norms, and branding, but not preference

Evaluations of Livestock Protection Dogs for Deterring Deer and Cattle Interactions

Bovine Tuberculosis (bovine TB) in northern Michigan has proven a dilemma necessitating aggressive measures including depopulation of livestock operations, culling of wildlife, banning the feeding of wildlife, and fencing livestock feed with high fences. Bovine TB is believed to be transmitted from white-tailed deer (Odocoileus virginianus) to domestic cattle through feces, urine, saliva, and nasal secretions on contaminated feed (indirect transmission) and from animal to animal (direct transmission). Effective methods for excluding deer from cattle enclosures would minimize the potential for indirect and direct transmission of bovine TB between infected deer and cattle. We combined an idea used historically in Europe to control predation on sheep with the concept of modem frightening devices that often fall short when the motivation of offending species is high. The use of livestock protection dogs (LPDs) seemed like an ideal solution in controlling the transmission of bovine TB from white-tailed deer to cattle. We felt LPDs raised and bonded with cattle would reduce use of cattle pastures and cattle feed by deer and minimize contact between deer and cattle, thereby reducing the potential for the transmission of bovine TB. We evaluated 4 LPDs over a 5-month period utilizing 2 primary data collection methods (direct observations and motion-activated video) on farmed deer facilities in Michigan. Following the initial evaluation of the LPDs, we relocated the dogs to working livestock operations in Michigan for further evaluation to gain an understanding of their practicality and long-term efficacy. Pastures protected by dogs had fewer intrusions by deer, fewer contacts (within 5m) between deer and cattle, and lower use of cattle feed by deer. Overall, we successfully decreased the potential for disease transmission with 66% fewer intrusions by deer into protected pastures, 96% fewer contacts (within 5m) between deer and cattle, and 100% lower use of cattle feed by deer (based of observation data). Livestock protection dogs were more effective in protecting animals and their immediate surroundings than excluding animals from entire study pastures. We found a strong treatment effect within the High Density Site; while within the Very High Density Site, we had high variability within intrusion rates at protected pastures and relatively low use of unprotected pastures resulting in no significant treatment effect. In conclusion, when properly trained and confined with the protected animals, LPDs minimize the potential for livestock to contract bovine TB from infected deer

Shape of the northern hemisphere of Mars from the Mars Orbiter Laser Altimeter (MOLA)

Eighteen profiles of ∼N-S-trending topography from the Mars Orbiter Laser Altimeter (MOLA) are used to analyze the shape of Mars' northern hemisphere. MOLA observations show smaller northern hemisphere flattening than previously thought. The hypsometric distribution is narrowly peaked with >20% of the surface lying within 200 m of the mean elevation. Low elevation correlates with low surface roughness, but the elevation and roughness may reflect different mechanisms. Bouguer gravity indicates less variability in crustal thickness and/or lateral density structure than previously expected. The 3.1-km offset between centers of mass and figure along the polar axis results in a pole-to-equator slope at all longitudes. The N-S slope distribution also shows a subtle longitude-dependent variation that may represent the antipodal effect of the formation of Tharsis

Increased Risk of Chronic Wasting Disease in Rocky Mountain Elk Associated With Decreased Magnesium and Increased Manganese in Brain Tissue

Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) of Rocky Mountain elk in North America. Recent studies suggest that tissue and blood mineral levels may be valuable in assessing TSE infection in sheep and cattle. The objectives of this study were to examine baseline levels of copper, manganese, magnesium, zinc, selenium, and molybdenum in the brains of Rocky Mountain elk with differing prion genotypes and to assess the association of mineral levels with CWD infection. Elk with leucine at prion position 132 had significantly lower magnesium levels than elk with 2 copies of methionine. Chronic wasting disease-positive elk had significantly lower magnesium than control elk. The incorporation of manganese levels in addition to magnesium significantly refined explanatory ability, even though manganese alone was not significantly associated with CWD. This study demonstrated that mineral analysis may provide an additional disease correlate for assessing CWD risk, particularly in conjunction with genotype

Topographic Rise in the Northern Smooth Plains of Mercury: Characteristics from Messenger Image and Altimetry Data and Candidate Modes of Origin

MESSENGER observations from orbit around Mercury have revealed that a large contiguous area of smooth plains occupies much of the high northern latitudes and covers an area in excess of approx.6% of the surface of the planet [1] (Fig. 1). Smooth surface morphology, embayment relationships, color data, candidate flow fronts, and a population of partly to wholly buried craters provide evidence for the volcanic origin of these plains and their emplacement in a flood lava mode to depths at least locally in excess of 1 km. The age of these plains is similar to that of plains associated with and postdating the Caloris impact basin, confirming that volcanism was a globally extensive process in the post-heavy bombardment history of Mercury [1]. No specific effusive vent structures, constructional volcanic edifices, or lava distributary features (leveed flow fronts or sinuous rilles) have been identified in the contiguous plains, although vent structures and evidence of high-effusion-rate flood eruptions are seen in adjacent areas [1]. Subsequent to the identification and mapping of the extensive north polar smooth plains, data from the Mercury Laser Altimeter (MLA) on MESSENGER revealed the presence of a broad topographic rise in the northern smooth plains that is ~1,000 km across and rises more than 1.5 km above the surrounding smooth plains [2] (Fig. 2). The purpose of this contribution is to characterize the northern plains rise and to outline a range of hypotheses for its origin

Temporal Dynamics of Chronic Inflammation on the Cecal Microbiota in IL-10-/- Mice

The intestinal microbiota is a critical component of mucosal health as evidenced by the fact that alterations in the taxonomic composition of the gastrointestinal microbiota are associated with inflammatory bowel diseases. To better understand how the progression of inflammation impacts the composition of the gastrointestinal microbiota, we used culture independent taxonomic profiling to identify temporal changes in the cecal microbiota of C3Bir IL-10-/- mice concomitantly with the onset and progression of colitis. This analysis revealed that IL-10-/- mice displayed a biphasic progression in disease severity, as evidenced by histopathological scores and cytokine production. Beginning at 4 weeks of age, pro-inflammatory cytokines including TNF-a, IFN-g, IL-6, G- CSF, and IL-1a as well as chemokines including RANTES and MIP-1a were elevated in the serum of IL-10-/- mice. By 19 weeks of age, the mice developed clinical signs of disease as evidenced by weight loss, which was accompanied by a significant increase in serum levels of KC and IL-17. While the overall diversity of the microbiota of both wild type and IL-10-/- were similar in young mice, the latter failed to increase in complexity as the mice matured and experienced changes in abundance of specific bacterial taxa that are associated with inflammatory bowel disease in humans. Collectively, these results reveal that there is a critical time in young mice between four to six weeks of age when inflammation and the associated immune responses adversely affect maturation of the microbiota

Size and frequency of natural forest disturbances and Amazon carbon balance

Forest inventory studies in the Amazon indicate a large terrestrial carbon sink. However, field plots may fail to represent forest mortality processes at landscape-scales of tropical forests. Here we characterize the frequency distribution of disturbance events in natural forests from 0.01 ha to 2,651 ha size throughout Amazonia using a novel combination of forest inventory, airborne lidar and satellite remote sensing data. We find that small-scale mortality events are responsible for aboveground biomass losses of B1.28 Pg C y 1 over the entire Amazon region. We also find that intermediate-scale disturbances account for losses of B0.01 Pg C y 1 , and that the largest-scale disturbances as a result of blow-downs only account for losses of B0.003 Pg C y 1 . Simulation of growth and mortality indicates that even when all carbon losses from intermediate and large-scale disturbances are considered, these are outweighed by the net biomass accumulation by tree growth, supporting the inference of an Amazon carbon sink

Deer guards and Bump Gates for Excluding White-Tailed Deer from Fenced Resources

White-tailed deer (Odocoileus virginianus) causing damage is a reoccurring theme in the realm of wildlife damage management, especially regarding human safety, disease transmission, and agricultural losses. Fences often are the only reliable long-term nonlethal means of controlling deer damage. The efficacy of fences, however, relies on their weakest link: human-operated gates. Although not overly time-consuming, the act of closing a gate appears to be a burden to individuals, resulting in open-access to an otherwise protected resource. We examined the efficacy of 2 alternatives to traditional gates to evaluate their potential to be used for excluding or containing deer. We evaluated a commercially available kit for mechanically opening and closing gates and a modified deer guard that resembles a common cattle guard but incorporates bearing-mounted rollers as cross members. The gate kit proved effective in restricting deer access to bait throughout the study, but, in supplemental evaluations, we observed excessive rates of functional failure. Deer guards reduced deer entry into exclosures, but efficacy declined with time as deer walked and jumped across guards. With some refining, both guards and gates have potential to be useful components of an integrated biosecurity strategy

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Nanoparticulate systems have emerged as valuable tools in vaccine delivery through their ability to efficiently deliver cargo, including proteins, to antigen presenting cells1-5. Internalization of nanoparticles (NP) by antigen presenting cells is a critical step in generating an effective immune response to the encapsulated antigen. To determine how changes in nanoparticle formulation impact function, we sought to develop a high throughput, quantitative experimental protocol that was compatible with detecting internalized nanoparticles as well as bacteria. To date, two independent techniques, microscopy and flow cytometry, have been the methods used to study the phagocytosis of nanoparticles. The high throughput nature of flow cytometry generates robust statistical data. However, due to low resolution, it fails to accurately quantify internalized versus cell bound nanoparticles. Microscopy generates images with high spatial resolution; however, it is time consuming and involves small sample sizes6-8. Multi-spectral imaging flow cytometry (MIFC) is a new technology that incorporates aspects of both microscopy and flow cytometry that performs multi-color spectral fluorescence and bright field imaging simultaneously through a laminar core. This capability provides an accurate analysis of fluorescent signal intensities and spatial relationships between different structures and cellular features at high speed. Herein, we describe a method utilizing MIFC to characterize the cell populations that have internalized polyanhydride nanoparticles or Salmonella enterica serovar Typhimurium. We also describe the preparation of nanoparticle suspensions, cell labeling, acquisition on an ImageStreamX system and analysis of the data using the IDEAS application. We also demonstrate the application of a technique that can be used to differentiate the internalization pathways for nanoparticles and bacteria by using cytochalasin-D as an inhibitor of actin-mediated phagocytosis