Deliberate Sequencing in the Music Classroom: A Curriculum to Guide Undergraduate Music Education Majors in Music Instruction

The content and structure of courses designed to train potential teachers are critical to such individuals\u27 success. Instructors of these courses should assess which skills are required of pre-service teacher candidates. Researchers have conducted studies over the years to decide what skills are needed for successful teaching. Learning cannot occur without an instructional sequence. Undergraduate music education majors are often not instructed on sequencing methods or have a chance to practice instructional sequencing before their student teaching placement. Research studies have shown that teachers who sequence their instructional delivery and following the complete teaching cycle are considered more effective in the classroom. The curriculum “Deliberate Sequencing in the Music Classroom,” was designed as a result of the research of Yarbrough and Price, Duke, and Ericsson to assist undergraduate music education majors become more effective in their instructional delivery. This document reviews literature on instructional sequencing and deliberate practice and provides the research findings that serve as the foundation for the attached curriculum

Phenotypic Plasticity within the Native Perennial Grass, Poa secunda (Sandberg bluegrass), an Important Component of the Western United States Sagebrush Grassland Vegetation

Native plant communities of the Nevada Great Basin are experiencing a rapid shift from native perennial grasses to invasive annual grasses. The ability to respond to competition through root plasticity may be an important trait for survival of native plants in invaded systems. I investigated the following questions about Poa secunda, a common native perennial grass in sagebrush ecosystems: 1) Can P. secunda respond plastically to directly-manipulated nutrient availability? 2) Does P. secunda respond to the presence of B. tectorum? and 3) Are these plastic responses adaptive? For the nutrient experiment, ten seeds from twenty families were sown individually into greenhouse pots, where one P. secunda family is defined as one individual plant. Within each family, five individuals received a low nutrient treatment, and five a high nutrient treatment. Plants were harvested fifty days after emergence. Roots were rinsed, clipped from leaf mass, and digitally scanned. For the competition experiment, twenty seeds from forty-eight families were sown individually into small and large greenhouse pots and one seed of B. tectorum was added to half of the pots. Plants were harvested sixty-seven days after emergence (early harvest), and after one growing season (late harvest) for the competition experiment. Roots were rinsed, clipped from their leaf mass and digitally scanned in a similar manner as the nutrient experiment. For both the nutrient and the competition experiments, measurements included total biomass, root to shoot ratio, leaf number, specific root length (SRL), percent allocation to different root diameter size classes, and plasticity in allocation to these same root diameter classes. In addition change in percent allocation to different root diameter size classes was calculated for plants in response to both low nutrients and competition from B. tectorum. Measures were correlated with plant performance measured as CPI (competitive performance index) and total biomass to determine if they were adaptive. Finally, to determine if plastic changes were simply a result of plant size or represented a change in strategy due to different growing environments, traits were assessed for "active" or "passive" plasticity by accounting for ontogenetic drift. In response to low nutrient treatment, P. secunda showed a 27% decrease in biomass (P2.0mm; P=0.04). Families differed in their response to low nutrients, and the nutrient by family interaction was significant for total biomass (P=0.0059). The nutrient experiment results demonstrated that P. secunda has the ability to allocate biomass to the production of more fine roots in response to low resource environments. In response to competition with B. tectorum for early harvest plants, P. secunda showed a 46% decrease in total biomass (P2.0mm; P<0.0001). Families differed in their response to traits measured, and the treatment by family interaction was significant for leaf number (P=0.0071) and percent allocation to different root diameter size classes (P=0.0001). The shift towards the production of finer root class 0.2-0.3mm and a higher root to shoot ratio may be an adaptively plastic response to competition with B. tectorum. For late harvest plants, P. secunda produced 88% less biomass (P<0.0001) and had 44% higher root to shoot ratio (P<0.0001) in response to competition. Families different in their root to shoot ratio (P<0.0001) and in their percent allocation to different root diameter size classes (P<0.0001). The treatment by family interaction, and the location by treatment interaction was significant for percent allocation to different root diameter size classes (P<0.0001). Plants allocated overall more biomass to fine root diameter size classes (0.1mm through 0.4mm) under competition (P<0.0001). In contrast to early harvest, allocation to coarse roots was adaptive in the late harvest. When ontogenetic drift was assessed, results show that P. secunda had a higher root to shoot ratio and allocated more resources to coarse roots relative to their size for some traits measured. This shift in allocation from producing less fine to more coarse roots may suggest a shift towards an adaptively plastic response to competition with B. tectorum. These results also suggest that P. secunda may have the ability to adaptively respond to invasion from B. tectorum through phenotypic plasticity

Shrinking the Malaria Map: A Prospectus on Malaria Elimination

\ud Thirty-nine countries across the world are making progress toward malaria elimination. Some are committed to nationwide elimination, while others are pursuing spatially progressive elimination within their borders. Influential donor and multilateral organizations are supporting their goals of achieving malaria-free status. With elimination back on the global agenda, countries face a myriad of questions. Should they change their programs to eliminate rather than control malaria? What tools are available? What policies need to be put into place? How will they benefit from elimination? Unfortunately, answers to these questions, and resources for agencies and country program managers considering or pursuing elimination, are scarce. The 39 eliminating countries are all positioned along the endemic margins of the disease, yet they naturally experience a variety of country characteristics and epidemiologies that make their malaria situations different from one another. The Malaria Elimination Group (MEG) and this Prospectus recognize\ud that there is no single solution, strategy, or time line that will be appropriate for every country, and each is encouraged to initiate a comprehensive evaluation of its readiness and strategy for elimination. The Prospectus is designed to guide countries in conducting these assessments. The Prospectus provides detailed and informed discussion on the practical means of achieving and sustaining zero transmission. It is designed as a road map, providing direction and options from which to choose an appropriate path. As on all maps, the destination is clearly marked, but the possible routes to reach it are numerous. The Prospectus is divided into two sections: Section 1 Eliminating Malaria comprises four chapters covering the strategic components important to the periods before, during, and after an elimination program. Section 2 Tools for the Job, comprises six chapters that outline basic information about how interventions in an elimination program will be different from those in a control setting. Chapter 1, Making the Decision, evaluates the issues that a country should consider when deciding whether or not to eliminate malaria. The chapter begins with a discussion about the quantitative and qualitative benefits that a country could expect from eliminating malaria and then recommends a thorough feasibility assessment. The feasibility assessment is based on three major components: operational, technical, and financial feasibility. Cross-border and regional collaboration is a key subject in this chapter. Chapter 2, Getting to Zero, describes changes that programs must consider when moving from sustained control to an elimination goal. The key strategic issues that must be addressed are considered, including supply chains, surveillance systems, intersectoral collaboration, political will, and legislative framework. Cross-border collaboration is again a key component in Getting to Zero. Chapter 3, Holding the Line, provides recommendations on how to conduct an assessment of two key factors that will affect preventing the reemergence of malaria once transmission is interrupted: outbreak risk and importation risk. The chapter emphasizes the need for a strong surveillance system in order to prevent and, if necessary, respond to imported cases. Chapter 4, Financing Elimination, reviews the cost-effectiveness of elimination as compared with sustained control and then presents the costs of selected elimination programs as examples. It evaluates four innovative financing mechanisms that must support elimination, emphasizing the need for predictable and stable financing. Case studies from Swaziland and two provinces in China are provided. Chapter 5, Understanding Malaria, considers malaria from the point of view of elimination and provides a concise overview of the current burden of the disease, malaria transmission, and the available interventions that can be used in an elimination program. Chapter 6, Learning from History, extracts important lessons from the Global Malaria Eradication Program and analyzes some elimination efforts that were successful and some that were unsuccessful. The chapter also reviews how the malaria map has been shrinking since 1900. xiv A Prosp ectus on Mala ria Elimi natio n\ud Chapter 7, Measuring Malaria for Elimination, provides a precise language for discussing malaria and gives the elimination discussion a quantitative structure. The chapter also describes the role of epidemiological theory and mathematical modeling in defining and updating an elimination agenda for malaria. Chapter 8, Killing the Parasite, outlines the importance of case detection and management in an elimination setting. Options for diagnosis, the hidden challenge of Plasmodium vivax in an elimination setting, and the impact of immunity are all discussed. Chapter 9, Suppressing the Vector, explores vector control, a necessary element of any malaria program. It considers optimal methods available to interrupt transmission and discusses potential changes, such as insecticide resistance, that may affect elimination efforts. Chapter 10, Identifying the Gaps — What We Need to Know, reviews the gaps in our understanding of what is required for elimination. The chapter outlines a short-term research agenda with a focus on the operational needs that countries are facing today. The Prospectus reviews the operational, technical, and financial feasibility for those working on the front lines and considers whether, when, and how to eliminate malaria. A companion document, A Guide on Malaria Elimination for Policy Makers, is provided for those countries or agencies whose responsibility is primarily to make the policy decisions on whether to pursue or support a malaria elimination strategy. The Guide is available at www.malaria eliminationgroup.org

\u3ci\u3eThree Strangers\u3c/i\u3e / \u3ci\u3eA Fable\u3c/i\u3e

Three Strangers: There were once three strangers upon this earth Whose odd gifts of power brought little mirth. A Fable: \u27If you build it, he will come.\u27 Zounds! I had the most extraordinary sense of deja vu watching Field of Dreams

The T cell antigen receptor complex expressed on normal peripheral blood CD4-, CD8- T lymphocytes. A CD3-associated disulfide-linked gamma chain heterodimer.

IL-2-dependent cell lines were established from normal peripheral blood T lymphocytes that express neither CD4 nor CD8 differentiation antigens. CD3+,4-,8- cell lines from 15 different donors failed to react with WT31, an mAb directed against the T cell antigen receptor alpha/beta heterodimer. Anti-Leu-4 mAb was used to isolate the CD3/T cell antigen receptor complex from 125I-labeled CD3+,4-,8- (WT31-) T cells. Using detergent conditions that preserved the CD3/T cell antigen receptor complex, an approximately 90 kD disulfide-linked heterodimer, composed of approximately 45- and approximately 40- (or approximately 37-) kD subunits, was coimmunoprecipitated with the invariant 20-29-kD CD3 complex. Analysis of these components by nonequilibrium pH gradient electrophoresis indicated that the approximately 40-kD and approximately 37-kD subunits were similar, and quite distinct from the more basic approximately 45-kD subunit. None of these three subunits reacted with an antibody directed against a beta chain framework epitope. Heteroantiserum against a T cell receptor gamma chain peptide specifically reacted with both the approximately 37- and approximately 40-kD CD3-associated proteins, but not with the approximately 45-kD subunit. CD3+,4-,8- cells failed to transcribe substantial amounts of functional 1.3-kb beta or 1.6-kb alpha mRNA, but produced abundant 1.6-kb gamma mRNA. Southern blot analysis revealed that these CD3+,4-,8- cell lines rearranged both gamma and beta genes, and indicated that the populations were polyclonal. The expression of a CD3-associated disulfide-linked heterodimer on CD3+,4-,8- T cell lines established from normal, adult peripheral blood contrasts with prior reports describing a CD3-associated non-disulfide-linked heterodimer on CD3+/WT31- cell lines established from thymus and peripheral blood obtained from patients with immunodeficiency diseases. We propose that this discrepancy may be explained by preferential usage of the two C gamma genes in T lymphocytes

Closed-Loop Recyclable Plastics from Poly(ethyl Cyanoacrylate)

Ethyl cyanoacrylate is a highly reactive monomer that has been used nearly exclusively to make Super Glue and related fast-setting adhesives. Here, we describe transformation of this highly abundant, readily available monomer into a closed-loop recyclable plastic that could supplant currently used (and often unrecycled/unrecyclable) plastics, such as poly(styrene). We report polymerization conditions, plastic-processing methods, and plastic-recycling protocols for poly(ethyl cyanoacrylate) plastics that make the Super Glue monomer a viable starting material for a next generation of closed-loop recyclable plastics. The processes described are scalable, and the plastics can be recycled in a closed-loop process with \u3e90% yields, even when combined with a heterogeneous mixture of other types of plastic

Identification of LDH-A as a therapeutic target for cancer cell killing via (i) p53/NAD(H)-dependent and (ii) p53 independent pathways

Most cancer cells use aerobic glycolysis to fuel their growth. The enzyme lactate dehydrogenase-A (LDH-A) is key to cancer’s glycolytic phenotype, catalysing the regeneration of nicotinamide adenine dinucleotide (NAD þ ) from reduced nicotinamide adenine dinucleotide (NADH) necessary to sustain glycolysis. As such, LDH-A is a promising target for anticancer therapy. Here we ask if the tumour suppressor p53, a major regulator of cellular metabolism, influences the response of cancer cells to LDH-A suppression. LDH-A knockdown by RNA interference (RNAi) induced cancer cell death in p53 wild-type, mutant and p53-null human cancer cell lines, indicating that endogenous LDH-A promotes cancer cell survival irrespective of cancer cell p53 status. Unexpectedly,however,weuncoveredanovelroleforp53intheregulationofcancercellNADþ anditsreducedformNADH.Thus, LDH-A silencing by RNAi, or its inhibition using a small-molecule inhibitor, resulted in a p53-dependent increase in the cancer cell ratioofNADH:NADþ.Thiseffectwasspecificforp53þ/þ cancercellsandcorrelatedwith(i)reducedactivityofNADþ-dependent deacetylase sirtuin 1 (SIRT1) and (ii) an increase in acetylated p53, a known target of SIRT1 deacetylation activity. In addition, activation of the redox-sensitive anticancer drug EO9 was enhanced selectively in p53 þ / þ cancer cells, attributable to increased activity of NAD(P)H-dependent oxidoreductase NQO1 (NAD(P)H quinone oxidoreductase 1). Suppressing LDH-A increased EO9-inducedDNAdamageinp53þ/þ cancercells,butimportantlyhadnoadditiveeffectinnon-cancercells.Ourresultsidentifya unique strategy by which the NADH/NADþ cellular redox status can be modulated in a cancer-specific, p53-dependent manner and we show that this can impact upon the activity of important NAD(H)-dependent enzymes. To summarise, this work indicates two distinct mechanisms by which suppressing LDH-A could potentially be used to kill cancer cells selectively, (i) through induction of apoptosis, irrespective of cancer cell p53 status and (ii) as a part of a combinatorial approach with redox-sensitive anticancer drugs via a novel p53/NAD(H)-dependent mechanism

Speech-Language Pathology Student Anxiety, Expectations, and Needs During Clinical Practicum

The current study investigates clinical supervision with regard to millennial student clinician expectations, needs, and anxiety related to the supervisory process. Seven graduate students in speech-language pathology completed questionnaires at six points during their graduate education – The Student Clinician Anxiety Test (Sleight, 1985), as well as Larson’s Supervisory Expectations Rating Scale and Supervisory Needs Rating Scale (Larson, 1981). Results indicated that supervisory needs and expectations were significantly greater pre-practicum when compared to post-externship, with no significant changes in student clinician anxiety noted across six semesters of graduate training. Student clinicians demonstrated less anxiety than Sleight’s data, but had greater needs and expectations than Larson’s data. The difference in anxiety may be a reflection of the cited confidence of millennials. The possibility also exists that supervisors have shifted their methods to incorporate recommended practices, promoting decreased anxiety. The higher level of expectation and needs is in keeping with the literature, which suggests that millennials live relatively structured lives and expect supervisors to provide structure and support to meet their learning needs

Advance care plans and hospitalized frail older adults: a systematic review.

INTRODUCTION: Frail older people are known to have low rates of advance care planning (ACP). Many frail patients prefer less aggressive treatment, but these preferences are often not known or respected. Frail patients often have multiple hospital admissions, potentially providing opportunities for ACP. OBJECTIVE: To systematically review the literature concerning ACP with frail older people in the acute hospital, with particular reference to: (1) Does ACP improve outcomes? (2) What are the views of patients, relatives and healthcare professionals regarding ACP? (3) Does ACP currently occur? (4) What are the facilitators and barriers to ACP? DESIGN: Systematic literature review and narrative synthesis. Electronic search of MEDLINE, CINAHL, ASSIA, PsycINFO and Embase databases from January 1990 to May 2019 inclusive. Studies in the acute setting of populations with a mean age >75 years, not focused on a disease-specific terminal condition were included. RESULTS: 16 133 articles were retrieved, 14 met inclusion criteria. No studies used an objective measure of frailty. One randomised controlled trial (RCT) found that ACP improves outcomes for older patients. Although 74%-84% of capacitous older inpatients are receptive to ACP, rates of ACP are 0%-5%; the reasons for this discrepancy have been little studied. The nature of ACP in clinical practice is unknown thus the extent to which it reflects the RCT intervention cannot be assessed. The outcomes that are important to patients are poorly understood and family and physician experiences have not been explored. CONCLUSIONS: A better understanding of this area could help to improve end-of-life care for frail older people. PROSPERO REGISTRATION NUMBER: CRD42017080246