Suprastrukturelle Organisation der Kollagenfibrillen in der Haut und der Cornea:Rolle der Kollagene I, II, III, V und XI

Die lichtdurchlässige Cornea enthält lamellar organisierte, dünne Kollagen I/V-Fibrillen. Durchmesser und Zusammensetzung dermaler Kollagen I/III/V-Fibrillen sind hingegen heterogen. Zusätzlich zu seiner Funktion als Nukleationskeim für die Fibrillogenese, wurde Kollagen V in epithelialen Verankerungszonen der Haut und der Cornea gefunden. Dabei war das unmaskierte N-Propeptid der alpha 1-Kette des Kollagen V mit der alpha 5-Kette des Kollagen VI kolokalisiert. Dies führt zu der Annahme, dass Kollagen V zusammen mit Kollagen VI ein Bestandteil mikrofibrillärer Netzwerke der epithelialen Verankerungszonen ist. Außerdem wurden im Stroma der adulten Cornea dicke Kollagen II/III-Fibrillen identifiziert, die eine möglicherweise altersunabhängige Rolle bei der Aufrechterhaltung der cornealen Integrität spielen. Die optischen Eigenschaften der Cornea werden demnach durch die geringe Anzahl dicker Fibrillen nur unerheblich beeinträchtigt

Inhalative IL-10 Attenuates Pulmonary Inflammation following Hemorrhagic Shock without Major Alterations of the Systemic Inflammatory Response

Several studies report immunomodulatory effects of endogenous IL-10 after trauma. The present study investigates the effect of inhalative IL-10 administration on systemic and pulmonary inflammation in hemorrhagic shock. Male C57/BL6 mice (8 animals per group) were subjected to pressure-controlled hemorrhagic shock for 1.5 hrs followed by resuscitation and inhalative administration of either 50 μL PBS (Shock group) or 50 μg/kg recombinant mouse IL-10 dissolved in 50 μL PBS (Shock + IL-10 group). Animals were sacrificed after 4.5 hrs of recovery and serum IL-6, IL-10, KC, and MCP-1 concentrations were measured with ELISA kits. Acute pulmonary inflammation was assessed by pulmonary myeloperoxidase (MPO) activity and pulmonary H&E histopathology. Inhalative IL-10 administration decreased pulmonary inflammation without altering the systemic concentrations of IL-6, IL-10, and KC. Serum MCP-1 levels were significantly reduced following inhalative IL-10 administration. These findings suggest that inhalative IL-10 administration may modulate the pulmonary microenvironment without major alterations of the systemic inflammatory response, thus minimizing the potential susceptibility to infection and sepsis

Интенсификация возделывания картофеля

В статье описаны требования к качеству посадочного материала. Проведен анализ существующих технологий посадки картофеля. Предложена модернизированная конструкция посадочной машины и соответственно усовершенствованная технология посадки картофеля.The article describes the requirements for the quality of planting material. The analysis of existing potato planting technologies is carried out. A modernized planting machine design and, correspondingly, an improved technology for planting potatoes are proposed

Совершенствование системы нормирования труда рабочих

Объект исследования - ЗАО "СИБУР-Транс". Предмет исследования – система нормирования труда на предприятия. Цель ВКР ? совершенствование системы нормирования труда на предприятии для активизации трудового потенциала на повышение производительности труда работников.The object of study - CJSC "SIBUR-TRANS". Subject of research - the system of regulation of labor in the enterprise. The purpose of WRC-improving the system of regulation of labor in the enterprise to enhance the labor potential to increase productivity of workers

Impact of Right Atrial Physiology on Heart Failure and Adverse Events after Myocardial Infarction

Background: Right ventricular (RV) function is a known predictor of adverse events in heart failure and following acute myocardial infarction (AMI). While right atrial (RA) involvement is well characterized in pulmonary arterial hypertension, its relative contributions to adverse events following AMI especially in patients with heart failure and congestion need further evaluation. Methods: In this cardiovascular magnetic resonance (CMR)-substudy of AIDA STEMI and TATORT NSTEMI, 1235 AMI patients underwent CMR after primary percutaneous coronary intervention (PCI) in 15 centers across Germany (n = 795 with ST-elevation myocardial infarction and 440 with non-ST-elevation MI). Right atrial (RA) performance was evaluated using CMR myocardial feature tracking (CMR-FT) for the assessment of RA reservoir (total strain εs), conduit (passive strain εe), booster pump function (active strain εa), and associated strain rates (SR) in a blinded core-laboratory. The primary endpoint was the occurrence of major adverse cardiac events (MACE) 12 months post AMI. Results: RA reservoir (εs p = 0.061, SRs p = 0.049) and conduit functions (εe p = 0.006, SRe p = 0.030) were impaired in patients with MACE as opposed to RA booster pump (εa p = 0.579, SRa p = 0.118) and RA volume index (p = 0.866). RA conduit function was associated with the clinical onset of heart failure and MACE independently of RV systolic function and atrial fibrillation (AF) (multivariable analysis hazard ratio 0.95, 95% confidence interval 0.92 to 0.99, p = 0.009), while RV systolic function and AF were not independent prognosticators. Furthermore, RA conduit strain identified low- and high-risk groups within patients with reduced RV systolic function (p = 0.019 on log rank testing). Conclusions: RA impairment is a distinct feature and independent risk factor in patients following AMI and can be easily assessed using CMR-FT-derived quantification of RA strain

miTuner - a kit for microRNA based gene expression tuning in mammalian cells

The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous microRNAs or a combination of both

A conserved population of MHC II-restricted, innate-like, commensal-reactive T cells in the gut of humans and mice

Interactions with commensal microbes shape host immunity on multiple levels and play a pivotal role in human health and disease. Tissue-dwelling, antigen-specific T cells are poised to respond to local insults, making their phenotype important in the relationship between host and microbes. Here we show that MHC-II restricted, commensal-reactive T cells in the colon of both humans and mice acquire transcriptional and functional characteristics associated with innate-like T cells. This cell population is abundant and conserved in the human and murine colon and endowed with polyfunctional effector properties spanning classic Th1- and Th17-cytokines, cytotoxic molecules, and regulators of epithelial homeostasis. T cells with this phenotype are increased in ulcerative colitis patients, and their presence aggravates pathology in dextran sodium sulphate-treated mice, pointing towards a pathogenic role in colitis. Our findings add to the expanding spectrum of innate-like immune cells positioned at the frontline of intestinal immune surveillance, capable of acting as sentinels of microbes and the local cytokine milieu

Acute adverse events in cardiac MR imaging with gadolinium-based contrast agents:results from the European Society of Cardiovascular Radiology (ESCR) MRCT Registry in 72,839 patients

International audienc