Watershed Outreach Professionals' Behavior Change Practices, Challenges, and Needs: Insights and Recommendations for the Chesapeake Bay Trust

This project was conducted in collaboration with a Maryland non-profit grant-making organization, the Chesapeake Bay Trust (CBT). The goal of the project was to determine how to assist local organizations in implementing environmental outreach programs (EOPs) that foster environmentally responsible behaviors (ERB) within the Chesapeake Bay Watershed. Based on findings from a review of conservation psychology literature, interviews with outreach program leaders, and observations of EOPs, the team designed and administered a survey to the CBT grant applicants (n=108, r=55%). The survey determined the EOP practices, challenges, and needs of these organizations. Survey results demonstrated that the majority of respondents’ organizations seek to motivate individuals to protect the Bay (97%) and that their EOPs have behavioral objectives (62%). Respondents reported that they motivate people in variety of ways, such as raising awareness (91 %). Their EOPs, however, also demonstrated misperceptions about how to achieve behavior change, for example suggesting that raising awareness will lead to ERB. Responses further revealed that many organizations are incorporating behavior change strategies and outreach best practices, such as targeting audiences (76%) and conducting internal evaluations (78%). Respondents, however, face challenges in implementing EOPs including: recruiting audiences not already environmentally motivated (53%); intentionally using behavior change strategies (52%); and developing EOPs with limited resources (48%). Finally, respondents felt their EOPs would most benefit from increased collaboration with other organizations (73%), opportunities to learn more about evaluation (63%), and training in how to incorporate academic research on ERB (53%). These survey results, as well as interview, observation, grant review, and presentation findings, led to the development of recommendations for the CBT and other funders interested in supporting EOPs to motivate ERB. Recommendations focused on facilitating effective program design include, addressing behavior change misconceptions, offering opportunities to learn more about behavior change, and developing outreach best practices skills, such as audience targeting and assessment. The recommendations also focus on ways to ensure that the needs of under-resourced grantees are met by encouraging collaboration and providing user-friendly, Bay-specific resources, such as the Rapid Assessment tool and guide to Strategies for Motivating Watershed Behavior created by the team.Master of ScienceNatural Resources and EnvironmentUniversity of Michiganhttp://deepblue.lib.umich.edu/bitstream/2027.42/90869/1/Watershed Outreach Professionals' Behavior Change Practices, Challenges, and Needs[1].pd

Shared Spaces as Authenticity: Exploring the Connectedness of the Physical Environments of Microstreamers and their Audience

This work examines how the on-camera environments of small streamers with extremely limited audiences (i.e. microstreamers) generate a form of authenticity and charm directly from the unstaged nature of said environments, and through the multi-purpose nature of these locations. While much of the current research on streaming has focused on larger, more professionalized (and monetized) activity, the microstreams explored here are significant in that they create a very different sense of audience engagement. The combination of (a) the unstaged nature of microstreaming environments, combined with (b) unscripted and unplanned actors and interruptions (pets, other members of the household, etc.) as well as (c) widely varying production values that range from nonexistent to low-budget mimicry of more professionalized streamers work together to generate a kind of intimacy that is consciously or unconsciously leveraged by the streamer themselves. In their failure to successfully demarcate frontstage and backstage efforts, microstreamers successfully engage audience members in the messiness of life

Noelaerhabdaceae coccolithophores as recorders of ancient atmospheric CO₂

Understanding the relationship between carbon dioxide and global climate is critical for predicting the severity of future climate change resulting from ongoing anthropogenic CO₂ emissions. By the year 2100, atmospheric CO₂ concentrations may reach close to 1000 ppm. The impacts of such a massive perturbation to the carbon cycle will last for hundreds of thousands of years. Estimates for the magnitude of warming range between ~3 and ~12°C, depending upon the timescale considered and actual CO₂ trajectory. To better understand the likely impacts of the Anthropocene, we must examine how CO₂ and climate have varied in the past, particularly during warm periods in Earth history with CO₂ levels higher than modern. Studies of this nature require the use of geological archives to reconstruct past environmental conditions. The carbon isotope fractionation of marine algae recorded in alkenone biomarkers (εp) is one of the primary tools for reconstructing past atmospheric CO₂ variations. These molecules are produced by the Noelaerhabdaceae, a family of calcifying coccolithophorid algae that has flourished in the open ocean for tens of millions of years. Measurements of alkenone εp have offered critical insight into the evolution of Earth’s atmosphere. However, CO₂ reconstructions using alkenone εp in the Pleistocene seldom agree with the atmospheric CO₂ variations known from ice cores. The reasons for this incongruity are not well understood. Recent studies have provided a deeper understanding of the complex mechanisms affecting alkenone carbon isotope fractionation, suggesting that the conventional framework for interpreting these records in terms of CO₂ may require reconsideration. Quantitatively understanding the link between alkenone εp and CO₂ can provide new insights to the history of atmospheric CO₂ and global climate on Earth. Here I use an empirical approach to explore relationships between physiology, environment, and alkenone carbon isotope fractionation in order to quantify the influence of CO₂ on alkenone εp. I begin with both new data and a meta-analysis of previous controlled laboratory studies on one of the dominant extant alkenone-producing algae. These experiments identify and quantify the significant roles of CO₂, irradiance, and cell size on alkenone εp. I calibrate multiple linear regression models from these culture data and find that when irradiance is included, growth rate is an insignificant contributor to εp variability. I test this model in core-top sediments and in Pleistocene deep-sea sediment sequences when atmospheric CO₂ concentrations are known from ice-core gas bubble analysis. With paired measurements of coccolith size and alkenone carbon isotope ratios, I find that although there is no spatial relationship between εp and CO₂ in the modern ocean, alkenone εp can be modeled reasonably well in core-top sediments, suggesting that the culture-based model is faithfully capturing the sensitivity of εp to irradiance, CO₂ and cell size in the modern ocean. Using Pleistocene alkenone εp and coccolith size records, I identify locations best-suited for paleobarometry, finding that tropical sites with stable surface hydrography and low variability in incident irradiance are sites where CO₂ and cell size dominate the p signal during the late Pleistocene. I apply this model to a new deep-sea sediment record to reconstruct CO₂ changes over the last ~20 million years. This record exhibits a drop in atmospheric pCO₂ of ~600 µatm over this time period, and suggests that the global cooling and C4 grassland expansion in the Miocene were likely caused by pCO2 decline. Together, these results present a new way to understand alkenone εp variations through time and quantify past CO₂ changes

Midlatitude Temperature Variations in the Oligocene to Early Miocene

[EN]Antarctic ice sheet margin extent and the sensitivity of benthic δ18O to orbital forcing have varied on million-year timescales during the Oligocene to Early Miocene. However, few sea surface temperature (SST) records for this time interval exist to evaluate links between polar processes and mean temperature outside polar regions. Here, we present a new record of SST for the time interval 30 to 17 Ma derived from the long-chain alkenone unsaturation ratio ( urn:x-wiley:25724517:media:palo20775:palo20775-math-0001) at Integrated Ocean Drilling Program Site 1406A in the midlatitude North Atlantic. Results confirm that warm temperatures from 24°C to over 30°C prevailed in midlatitudes in this time and suggest a transition from colder early-middle Oligocene to warmer average conditions after 24.5 Ma. The global significance of this transition is highlighted by the coincidence with changes in the dominance from marine- to terrestrial-terminating ice sheets in the Ross Sea around Antarctica. The longest continuous section of the record (20.6 to 26.6 Ma) contains multiple 2 million-year cycles in SST, potentially paced by long obliquity modulation. Complex and temporally varying relationships are observed between North Atlantic SST and benthic δ18O in paired samples; significant covariation is only observed around the Oligocene-Miocene transition, coincident with a lower average marine ice extent. These North Atlantic urn:x-wiley:25724517:media:palo20775:palo20775-math-0002 temperature records provide a new context in which to examine the stability of climate and the Antarctic ice sheet during the Oligocene and early Miocene

Upregulation of phytoplankton carbon concentrating mechanisms during low CO2 glacial periods and implications for the phytoplankton pCO2 proxy

Unidad de excelencia María de Maeztu MdM-2015-0552Published alkenone εp records spanning known glacial pCO2 cycles show considerably less variability than predicted by the diffusive model for cellular carbon acquisition and isotope fractionation. We suggest this pattern is consistent with a systematic cellular enhancement of the carbon supply to photosynthesis via carbon concentrating mechanisms under the case of carbon limitation during low pCO2 glacial time periods, an effect also manifest under carbon limitation in experimental cultures of coccolithophores as well as diatoms. While the low-amplitude εp signal over glacial pCO2 cycles has led some to question the reliability of εp for reconstructing long-term pCO2, the [CO2]aq in the tropical oceans during glacial pCO2 minima represents the most extreme low CO2 conditions likely experienced by phytoplankton in the Cenozoic, and the strongest upregulation of carbon concentrating mechanisms. Using a statistical multilinear regression model, we quantitatively parse out the factors (namely light, growth rate, and [CO2]aq), that contribute to variation in εp in alkenone-producing algae, which confirms a much smaller dependence of εp on [CO2]aq in the low [CO2]aq range, than inferred from the hyperbolic form of the diffusive model. Application of the new statistical model to two published tropical εp records spanning the late Neogene produces much more dynamic pCO2 estimates than the conventional diffusive model and reveals a significant pCO2 decline over the last 15 Ma, which is broadly consistent with recent results from boron isotopes of foraminifera. The stable isotopic fractionation between coccolith calcite and seawater dissolved inorganic carbon (here Δcoccolith-DIC) also shows systematic variations over glacial-interglacial cycles which may, following future experimental constraints, help estimate the degree of upregulation of parts of the algal carbon concentrating mechanism over glacial cycles

Patients' continuing use of an online health record:a quantitative evaluation of 14,000 patient years of access data

BACKGROUND: Online access to all or part of their health records is widely demanded by patients and, where provided in form of patient portals, has been substantially used by at least subgroups of patients, particularly those with chronic disease. However, little is reported regarding the longer-term patient use of patient-accessible electronic health record services, which is important in allocating resources. Renal PatientView (RPV) is an established system that gives patients with chronic kidney disease access to live test results and information about their condition and treatment. It is available in most UK renal units with up to 75% of particular patient groups registered in some centers. We have analyzed patient use out to 4 years and investigated factors associated with more persistent use. OBJECTIVE: Our aim was to investigate RPV use by patients over time from initial registration in order to understand which patients choose to access RPV and the endurance of its appeal for different patient groups. METHODS: We analyzed an anonymized extract of the database underlying RPV containing information on patient registration and events including patient access and the arrival of new blood test results or letters that patients might wish to view. RESULTS: At the time of the extract, there were 11,352 patients registered on RPV for 0-42 months (median 17). More than half of registrants became persistent users, logging in a median of 2.0 times each month over post-registration intervals of up to 42 months (median 18.9). Provision of assistance with first logon was strongly associated with becoming a persistent user, even at 3 years. Logons by persistent users occurred around the time of consultations/tests, strongly suggestive of patient engagement. While indices indicative of greater deprivation were the strongest determinants of non-participation, they had negligible influence on drop-out rates among established users. CONCLUSIONS: In this mature patient portal system, a large proportion of patients made regular use of their online health records over protracted periods. The patterns and timing of use indicate strong patient interest in detailed information such as recent test results and clinic letters. Supporting patients through the first steps of establishing access to their online records is associated with much higher rates of long-term use of RPV and likely would increase use of other electronic health records provided for patients with chronic disease

Spectrum of Mutations in the RPGR Gene That Are Identified in 20% of Families with X-Linked Retinitis Pigmentosa

SummaryThe RPGR (retinitis pigmentosa GTPase regulator) gene for RP3, the most frequent genetic subtype of X-linked retinitis pigmentosa (XLRP), has been shown to be mutated in 10%–15% of European XLRP patients. We have examined the RPGR gene for mutations in a cohort of 80 affected males from apparently unrelated XLRP families, by direct sequencing of the PCR-amplified products from the genomic DNA. Fifteen different putative disease-causing mutations were identified in 17 of the 80 families; these include four nonsense mutations, one missense mutation, six microdeletions, and four intronic-sequence substitutions resulting in splice defects. Most of the mutations were detected in the conserved N-terminal region of the RPGR protein, containing tandem repeats homologous to those present in the RCC-1 protein (a guanine nucleotide-exchange factor for Ran-GTPase). Our results indicate that mutations either in as yet uncharacterized sequences of the RPGR gene or in another gene located in its vicinity may be a more frequent cause of XLRP. The reported studies will be beneficial in establishing genotype-phenotype correlations and should lead to further investigations seeking to understand the mechanism of disease pathogenesis

Spectrum of Mutations in the RPGR Gene That Are Identified in 20% of Families with X-Linked Retinitis Pigmentosa

SummaryThe RPGR (retinitis pigmentosa GTPase regulator) gene for RP3, the most frequent genetic subtype of X-linked retinitis pigmentosa (XLRP), has been shown to be mutated in 10%–15% of European XLRP patients. We have examined the RPGR gene for mutations in a cohort of 80 affected males from apparently unrelated XLRP families, by direct sequencing of the PCR-amplified products from the genomic DNA. Fifteen different putative disease-causing mutations were identified in 17 of the 80 families; these include four nonsense mutations, one missense mutation, six microdeletions, and four intronic-sequence substitutions resulting in splice defects. Most of the mutations were detected in the conserved N-terminal region of the RPGR protein, containing tandem repeats homologous to those present in the RCC-1 protein (a guanine nucleotide-exchange factor for Ran-GTPase). Our results indicate that mutations either in as yet uncharacterized sequences of the RPGR gene or in another gene located in its vicinity may be a more frequent cause of XLRP. The reported studies will be beneficial in establishing genotype-phenotype correlations and should lead to further investigations seeking to understand the mechanism of disease pathogenesis

Do all bunions need surgery? An investigation of Google searches for Hallux Valgus

Introduction: Hallux valgus (HV) or a bunion is one of the most common forefoot deformities. Approximately one in four adults will develop HV with a higher prevalence in adult females. Given the high prevalence of HV along with the wide variety of non-surgical and surgical treatment options for it, we believe patients are likely turning to internet search engines for questions germane to HV. Previous orthopaedic investigations have used Google’s “People Also Ask” box to characterize frequently asked questions (FAQs) regarding total knee and hip arthroplasty. Yet, no such investigation has been conducted for HV. Using Google’s Frequently Asked Questions (FAQs), we sought to classify these questions as well as assess their levels quality and transparency.Methods: On October 9, 2022, we searched Google using these four phrases: “Hallux Valgus Treatment,” “Hallux Valgus Treatment Surgery,” “Bunion Treatment,” and “Bunion Surgery.” We refreshed search results until a minimum of 100 FAQs were produced; we extracted the sources of these 100 questions for content analysis. Information transparency was assessed using the Journal of the American Medical Association’s (JAMA) Benchmark tool, question classification was defined using the Rothwell Classification of Questions, and information quality was assessed using Brief DISCERN. This study was approved by an IRB.Results section: Our Google search returned 299 unique FAQs after removing duplicates and unrelated FAQs. The majority were classified as fact based questions (149/299, 49.8%), followed by value (92/299, 30.8%) and policy questions (58/299, 19.4%). Overall the most common topic searched was related to the evaluation of treatment or surgery (79/299, 26.4%). The frequent answer sources were medical practices (158/299, 52.8%), followed by commercial (69/299, 23.1%) and academic (38/299, 12.7%). The one-way analysis of variance revealed a significant difference in mean quality scores among the 5 source types (F= 54.49, P<.001) with medical practices averaging the worst score (12.1/30) compared to academic sources which were found to have the highest score (21.8/30).Discussion: Patients seeking online information concerning treatment options for HV appear to search Google for questions related to treatment efficacy and restrictions. The most common source type encountered by patients are small medical practices; these were found to have both poor transparency and poor quality. Our study has several limitations, JAMA benchmark and Brief DISCERN do not assess the accuracy of the information. Secondly, our study is cross-sectional in design limiting the generalizability of our findings to the time when the search was performed.Significance/Clinical Relevance: In order to increase the transparency and quality of online information regarding HV treatment, online sources should refer to established rubrics such as JAMA benchmark and Brief DISCERN when publishing online information. Physicians should be aware that patients are commonly searching for information related to the evaluation of treatments or surgeries for HV