Impacts of multiple stressors on a benthic foraminiferal community: a long-term experiment assessing response to ocean acidification, hypoxia and warming

© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Bernhard, J. M., Wit, J. C., Starczak, V. R., Beaudoin, D. J., Phalen, W. G., & McCorkle, D. C. Impacts of multiple stressors on a benthic foraminiferal community: a long-term experiment assessing response to ocean acidification, hypoxia and warming. Frontiers in Marine Science, 8, (2021): 643339, https://doi.org/10.3389/fmars.2021.643339.Ocean chemistry is changing as a result of human activities. Atmospheric carbon dioxide (CO2) concentrations are increasing, causing an increase in oceanic pCO2 that drives a decrease in oceanic pH, a process called ocean acidification (OA). Higher CO2 concentrations are also linked to rising global temperatures that can result in more stratified surface waters, reducing the exchange between surface and deep waters; this stronger stratification, along with nutrient pollution, contributes to an expansion of oxygen-depleted zones (so called hypoxia or deoxygenation). Determining the response of marine organisms to environmental changes is important for assessments of future ecosystem functioning. While many studies have assessed the impact of individual or paired stressors, fewer studies have assessed the combined impact of pCO2, O2, and temperature. A long-term experiment (∼10 months) with different treatments of these three stressors was conducted to determine their sole or combined impact on the abundance and survival of a benthic foraminiferal community collected from a continental-shelf site. Foraminifera are well suited to such study because of their small size, relatively rapid growth, varied mineralogies and physiologies. Inoculation materials were collected from a ∼77-m deep site south of Woods Hole, MA. Very fine sediments (<53 μm) were used as inoculum, to allow the entire community to respond. Thirty-eight morphologically identified taxa grew during the experiment. Multivariate statistical analysis indicates that hypoxia was the major driving factor distinguishing the yields, while warming was secondary. Species responses were not consistent, with different species being most abundant in different treatments. Some taxa grew in all of the triple-stressor samples. Results from the experiment suggest that foraminiferal species’ responses will vary considerably, with some being negatively impacted by predicted environmental changes, while other taxa will tolerate, and perhaps even benefit, from deoxygenation, warming and OA.This work was supported by the US NSF SEES-OA grant OCE-1219948 to JB and the Investment in Science Program at WHOI. DM also received support from the NSF Independent Research and Development Program

Technical Note : Towards resolving in situ, centimeter-scale location and timing of biomineralization in calcareous meiobenthos – the calcein–osmotic pump method

© The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Biogeosciences 12 (2015): 5515-5522, doi:10.5194/bg-12-5515-2015.Insights into oceanographic environmental conditions such as paleoproductivity, deep-water temperatures, salinity, ice volumes, and nutrient cycling have all been obtained from geochemical analyses of biomineralized carbonate of marine organisms. However, we cannot fully understand geochemical proxy incorporation and the fidelity of such in species until we better understand fundamental aspects of their ecology such as where and when these (micro)organisms calcify. Here, we present an innovative method using osmotic pumps and the fluorescent marker calcein to help identify where and when calcareous meiofauna calcify in situ. Method development initially involved juvenile quahogs (Mercenaria mercenaria); subsequent method refinement involved a neritic benthic foraminiferal community. Future applications of this method will allow determining the in situ growth rate in calcareous organisms and provide insights about microhabitats where paleoceanographically relevant benthic foraminifera actually calcify.This research was funded by WHOI’s Ocean Life Institute, WHOI’s Ocean and Climate Change Institute, by a Gori Fellowship (to F. Mezzo), The Investment in Science Fund at WHOI (to J. M. Bernhard) and the Robert W. Morse Chair for Excellence in Oceanography (to J. M. Bernhard). Ship time was provided by US NSF grant OCE-1219948 to J. M. Bernhard

Adapting without retreating : responses to shoreline change on an inlet-associated coastal beach

Author Posting. © The Author(s), 2017. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Coastal Management 45 (2017): 360-383, doi:10.1080/08920753.2017.1345607.Coastal barrier systems around the world are experiencing higher rates of flooding and shoreline erosion. Property owners on barriers have made significant financial investments in physical protections that shield their nearby properties from these hazards, constituting a type of adaptation to shoreline change. Factors that contribute to adaptation on Plum Island, a developed beach and dune system on the North Shore of Massachusetts, are investigated here. Plum Island experiences patterns of shoreline change that may be representative of many inlet-associated beaches, encompassing an equivocal and dynamically shifting mix of erosion and accretion. In the face of episodic floods and fleeting erosive events, and driven by a combination of strong northeast storms and cycles of erosion and accretion, the value of the average Plum Island residence increases by 34% for properties on the oceanfront where protection comprises a publicly constructed soft structure. Even in the face of state policies that ostensibly discourage physical protection as a means of adaptation, coastal communities face significant political and financial pressures to maintain existing protective structures or to allow contiguous groups of property owners to build new ones through collective action. These factors mitigate against adapting to shoreline change by retreating from the coast, thereby potentially increasing the adverse effects of coastal hazards.Support for this study was provided by NSF Grant Nos. OCE 1325430 and AGS 1518503 and NOAA Cooperative Agreement No. NA14OAR4170074

Genomic comparisons reveal biogeographic and anthropogenic impacts in the koala (Phascolarctos cinereus): a dietary-specialist species distributed across heterogeneous environments

The Australian koala is an iconic marsupial with highly specific dietary requirements distributed across heterogeneous environments, over a large geographic range. The distribution and genetic structure of koala populations has been heavily influenced by human actions, specifically habitat modification, hunting and translocation of koalas. There is currently limited information on population diversity and gene flow at a species-wide scale, or with consideration to the potential impacts of local adaptation. Using species-wide sampling across heterogeneous environments, and high-density genome-wide markers (SNPs and PAVs), we show that most koala populations display levels of diversity comparable to other outbred species, except for those populations impacted by population reductions. Genetic clustering analysis and phylogenetic reconstruction reveals a lack of support for current taxonomic classification of three koala subspecies, with only a single evolutionary significant unit supported. Furthermore, similar to 70% of genetic variance is accounted for at the individual level. The Sydney Basin region is highlighted as a unique reservoir of genetic diversity, having higher diversity levels (i.e., Blue Mountains region; AvHe(corr)-0.20, PL% = 68.6). Broad-scale population differentiation is primarily driven by an isolation by distance genetic structure model (49% of genetic variance), with clinal local adaptation corresponding to habitat bioregions. Signatures of selection were detected between bioregions, with no single region returning evidence of strong selection. The results of this study show that although the koala is widely considered to be a dietary-specialist species, this apparent specialisation has not limited the koala's ability to maintain gene flow and adapt across divergent environments as long as the required food source is available

Detection and Heterogeneity of Herpesviruses Causing Pacheco's Disease in Parrots

Pacheco's disease (PD) is a common, often fatal, disease of parrots. We cloned a virus isolate from a parrot that had characteristic lesions of PD. Three viral clones were partially sequenced, demonstrating that this virus was an alphaherpesvirus most closely related to the gallid herpesvirus 1. Five primer sets were developed from these sequences. The primer sets were used with PCR to screen tissues or tissue culture media suspected to contain viruses from 54 outbreaks of PD. The primer sets amplified DNA from all but one sample. Ten amplification patterns were detected, indicating that PD is caused by a genetically heterogeneous population of viruses. A single genetic variant (psittacid herpesvirus variant 1) amplified with all primer sets and was the most common virus variant (62.7%). A single primer set (23F) amplified DNA from all of the positive samples, suggesting that PCR could be used as a rapid postmortem assay for these viruses. PCR was found to be significantly more sensitive than tissue culture for the detection of psittacid herpesviruses

Adapting without retreating : responses to shoreline change on an inlet-associated coastal beach

Author Posting. © The Author(s), 2017. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Coastal Management 45 (2017): 360-383, doi:10.1080/08920753.2017.1345607.Coastal barrier systems around the world are experiencing higher rates of flooding and shoreline erosion. Property owners on barriers have made significant financial investments in physical protections that shield their nearby properties from these hazards, constituting a type of adaptation to shoreline change. Factors that contribute to adaptation on Plum Island, a developed beach and dune system on the North Shore of Massachusetts, are investigated here. Plum Island experiences patterns of shoreline change that may be representative of many inlet-associated beaches, encompassing an equivocal and dynamically shifting mix of erosion and accretion. In the face of episodic floods and fleeting erosive events, and driven by a combination of strong northeast storms and cycles of erosion and accretion, the value of the average Plum Island residence increases by 34% for properties on the oceanfront where protection comprises a publicly constructed soft structure. Even in the face of state policies that ostensibly discourage physical protection as a means of adaptation, coastal communities face significant political and financial pressures to maintain existing protective structures or to allow contiguous groups of property owners to build new ones through collective action. These factors mitigate against adapting to shoreline change by retreating from the coast, thereby potentially increasing the adverse effects of coastal hazards.Support for this study was provided by NSF Grant Nos. OCE 1325430 and AGS 1518503 and NOAA Cooperative Agreement No. NA14OAR4170074

Seawater carbonate chemistry and counts for foram propagule community

Ocean chemistry is changing as a result of human activities. Atmospheric carbon dioxide (CO2) concentrations are increasing, causing an increase in oceanic pCO2 that drives a decrease in oceanic pH, a process called ocean acidification (OA). Higher CO2 concentrations are also linked to rising global temperatures that can result in more stratified surface waters, reducing the exchange between surface and deep waters; this stronger stratification, along with nutrient pollution, contributes to an expansion of oxygen-depleted zones (so called hypoxia or deoxygenation). Determining the response of marine organisms to environmental changes is important for assessments of future ecosystem functioning. While many studies have assessed the impact of individual or paired stressors, fewer studies have assessed the combined impact of pCO2, O2, and temperature. A long-term experiment (10 months) with different treatments of these three stressors was conducted to determine their sole or combined impact on the abundance and survival of a benthic foraminiferal community collected from a continental-shelf site. Foraminifera are well suited to such study because of their small size, relatively rapid growth, varied mineralogies and physiologies. Inoculation materials were collected from a 77-m deep site south of Woods Hole, MA. Very fine sediments (<53 μm) were used as inoculum, to allow the entire community to respond. Thirty-eight morphologically identified taxa grew during the experiment. Multivariate statistical analysis indicates that hypoxia was the major driving factor distinguishing the yields, while warming was secondary. Species responses were not consistent, with different species being most abundant in different treatments. Some taxa grew in all of the triple-stressor samples. Results from the experiment suggest that foraminiferal species' responses will vary considerably, with some being negatively impacted by predicted environmental changes, while other taxa will tolerate, and perhaps even benefit, from deoxygenation, warming and OA