Jämförelse mellan tre indirekta metoder för att beräkna klövviltstätheter

Reliable species population estimates are important for population conservation and management. Information about population sizes is needed to introduce certain preservation and regulation approaches. Several different methods, both performed in the field and analytical, can be used to estimate species densities. The aim of this thesis was to evaluate densities of moose (Alces alces), red deer (Cervus elaphus), roe deer (Capreolus capreolus), and fallow deer (Dama dama) in a confined area in Västerbotten, Sweden, via three different non-invasive wildlife census methods: camera trapping, traditional dung pellet group counts, and DNA-based ID-ing of dung samples. Due to analytical problems in the lab, the latter method was excluded from any further analysis. Species densities were estimated via the Random Encounter Model (REM) from recorded pictures of camera traps. Further investigations from camera trap data were made to compare sex-ratio, day- and night-capture events, the influence of snow depth and the use of habitat type among the ungulate species. Density estimates of dung counts considered the defecation rate of each species and the accumulation period of dung. For moose, camera traps yielded a population estimate similar to dung counts. However, estimated densities from the dung count approach were much lower for all other three ungulate species than densities from the camera trapping. Even though the use of camera traps is more time consuming and costly, this method is evaluated as a trustworthy estimator of population sizes. In addition, further information about habitat associations, activity patterns, behaviour, or community structure can be successfully accomplished

Summer and winter browsing affect conifer growth differently: An experimental study in a multi-species ungulate community

Ungulate browsing has been studied for several decades in the northern hemisphere. However, studies have mainly focused on just one or two ungulate species, while rarely contrasting the relative effects of summer and winter browsing. This limits our understanding of the dynamics and effects of browsing in landscapes where ungulate species diversity is increasing. We conducted a seasonal exclosure experiment on former clear-cuts in a multi-species ungulate system in Sweden, to investigate the relative impacts of summer and winter browsing on the conifers Scots pine (Pinus sylvestris) and Norway spruce (Picea abies) at the tree species level. We tested for differences in individual conifer growth and demographic responses between Summer browsing, Winter browsing, No browsing, and Control treatment plots over a 4.5 year experimental period. We defined the demographic response as the distribution of conifers among different height classes. Individual growth rates and demographic responses of both conifer species were similar in the plots with No browsing (year-round exclosures) as in the Control plots with year-round browsing. Plots subject to Summer and Winter browsing differed in terms of their demographic response relative to plots with No browsing and Control plots; more stems reached taller height classes in the Summer and Winter browsing plots with slight differences between the conifer species. We discuss the different responses of Scots pine and Norway spruce considering their differences in palatability and their ability to tolerate plant-plant competition in a multi-species ungulate system, in light of potential associational effects. If fencing is intended to be used as a management practice to mitigate the impacts of deer browsing on conifer growth, our results suggest that a total exclusion of deer does not necessarily enhance conifer growth during the first years of regeneration

Predictors of browsing damage on commercial forests – A study linking nationwide management data

Population sizes and species distributions of wild ungulates in Europe have increased during the past decades, and continue to do so. As a result, browsing pressure in forests is increasing and concerns about the effects of increasingly common multi-species deer communities on forestry are rising. However, we currently lack an understanding of how the composition of deer communities affects browsing damage, particularly with respect to the role of species with different dietary requirements. Further, the relative importance of predictors of browsing damage in systems with multiple browsers remains elusive. Here, we used data from Swedish management, which is monitoring ungulates and their damage to forests, to test how deer densities, forage availability, and winter severity predict browsing damage on commercially important Scots pine (Pinus sylvestris) at national and regional scales. Moose (Alces alces) is the main browser of Scots pine, but competes with other deer over more preferred forage. During winter, a higher index of moose density was associated with higher browsing damage in northern Sweden, where there is low competition from other deer. In southern Sweden, competition from other deer is higher and a higher density index of roe deer (Capreolus capreolus) was associated with higher winter damage. However, there was no relationship between moose density and damage. We suggest that moose are forced to browse more pine due to interspecific competition over alternative forage resources in the south, yielding stronger relationships between densities of competing deer species and damage than between moose density and damage. Pine density, a proxy for forage availability, was an equally or more important determinant for browsing damage as deer density indices in most regions. Increasing pine density was associated with reduced browsing damage caused in winter in all regions and reduced damage caused in summer in central Sweden. Increased winter severity, as an index of snow depth, predicted increased winter damage in the northern region only. To reduce winter damage, our results suggest that management should consider deer densities and forage availability simultaneously, while adopting a multi-species approach. Results varied among regions, with densities of other deer being more relevant in the south and winter severity more relevant in the north. Management data did not predict summer damage well. Since national models did not capture regional variations, we conclude that management decisions and actions need to be tailored to the regional or local scale

Evaluating the outcomes of collaborative wildlife governance: The role of social-ecological system context and collaboration dynamics

The acknowledgement of uncertainty and complexity in social-ecological systems has increased the implementation of collaborative governance regimes for environmental issues. The performance of these new regimes to deliver favourable social and ecological outcomes must therefore be evaluated. We focus on the case of Swedish wildlife governance, which has a tradition of using collaborative elements. In relation to moose (Alces alces), these collaborative aspects were recently formalized in an amended policy. We aim to assess some aspects of this new regime's performance with respect to intermediate ecological outcomes (i.e. quota fulfilment). We use path analysis to test the causal effects of system context and collaboration dynamics on governance outcomes. Collaboration dynamics were assessed using a web-based survey sent to all stakeholders in Moose Management Groups (response rate = 82 %). Our originally specified model yielded a good fit (SRMR of .030 and robust TLI of .996) and explained 20 % of the variation in outcomes. Context variables revealed significant direct effects on collaboration dynamics and outcomes. Larger Moose Management Areas and fluctuations in forage availability required more time investment from actors, while high land use diversity and density of other ungulate species negatively affected moose quota fulfilment. Moose Management Groups that invested more time and perceived to have a good knowledge base achieved better quota fulfilment. Collaboration dynamics thus had a positive direct effect on outcomes. From a policy perspective, our results raise questions regarding institutional fit because context factors had significant negative effects on collaboration dynamics and the outcomes of the collaborative process

Browsing Damage on Scots Pine: Direct and Indirect Effects of Landscape Characteristics, Moose and Deer Populations

Reducing browsing damages from cervids (Cervidae) on economically valuable tree species is a challenging task in many countries. Apart from cervid abundance, landscape characteristics, such as forest composition, land use, forage availability and climatic conditions, may affect the degree of browsing through both direct and indirect effects. A better understanding of basic mechanisms in this complex system is needed to design efficient and convincing management strategies. Focusing on Sweden as a case, which has been widely studied using regression analyses only, we applied path analysis to test the validity of a model on the indirect and direct links between landscape characteristics, cervid populations, and browsing damages on Scots pine (Pinus sylvestris). Our results validated the tested model in which moose (Alces alces) density and pine availability directly influence browsing damages. Increasing amounts of pine forests, preferred deciduous trees, and young forest had positive direct effects on moose densities and thereby indirectly contributed to increased browsing damages. The density of smaller deer species showed no direct effect on browsing damages on pine. Path analysis corroborated our attempt to disentangle direct and indirect potential causal drivers of browsing damages and shows that the choice of statistical method may alter the understanding of mechanistic driving forces

A set of novel multiplex Taqman real-time PCRs for the detection of diarrhoeagenic Escherichia coli and its use in determining the prevalence of EPEC and EAEC in a university hospital

<p>Abstract</p> <p>Background</p> <p>Accurate measurement of the incidence of diarrhoeagenic <it>E. coli </it>in patients with diarrhoea is hindered by the current methods of detection and varies from country to country. In order to improve the diagnosis of diarrhoeagenic <it>E. coli </it>(DEC), we developed a set of multiplex TaqMan real-time PCRs designed to detect the respective pathogens from an overnight stool culture.</p> <p>Methods</p> <p>Over the period Jan. 2006 to Dec. 2006 all stool specimens (n = 1981) received were investigated for EPEC and EAEC.</p> <p>Results</p> <p>Of these, 371 specimens had no growth of <it>Enterobacteriaceae</it>. Of the remaining 1610 specimens 144 (8,9%) were positive for EPEC and 78 (4,8%) positive for EAEC. Among the EPEC positive stool specimens 28 (19,4%) were received from the tropical diseases unit, 49 (34%) from the paediatric dept. and 67 (46,5%) from the remainder of the wards. The EAEC were distributed as follows: 39 (50%) - tropical diseases, 19 (24,4%) -paediatrics and 20 (25,6%) other wards. Proportionately more EAEC and EPEC were found in children less than 3 years of age than other age groups. In only 22,2% of the detected EPEC and 23% of EAEC was the investigation requested by hospital staff.</p> <p>Conclusions</p> <p>This is, to our knowledge, the first study using a multiplex TaqMan PCR for the successful detection of diarrhoeagenic <it>E. coli</it>. In conclusion, due to the high prevalence of DEC detected, investigation of EPEC and EAEC should be recommended as a routine diagnostic test for patients with infectious diarrhoea.</p

Simulation-based medical education for Ambulance Jet and Helicopter Emergency Medical Services: A program description and evaluation

Introduction: In aviation, crew resource management trainings are established methods to enhance safety, a method that also gained popularity in medicine. In 2015, the Swiss Air Rescue (Rega) Helicopter Emergency Medical Services decided to start a simulation-based medical education program for its helicopter and ambulance jet crews (emergency physicians, paramedics/flight nurses and pilots). The aim of this program was to improve technical skills and the application of human factors during rescue missions. This report shows a five-year summary of the participants’ course evaluation. Methods: A 1-day high-fidelity simulation on crisis resource management with video-assisted debriefing took place at 3 centres, two in Switzerland; one in Germany. Crew members participated once per year. Simulation covered critical situations in the helicopter or jet, during handovers at an intensive care unit or in ambulances. Extra Corporeal Membrane Oxygenation and Intra-Aortic Balloon Pump use was simulated during helicopter transports. Additionally, four times per year flight crews rehearsed basic and advanced life support skills using low-fidelity equipment between missions. Participants answered an anonymized course evaluation survey. Answers were rated on a Numeric Rating Scale ranging from 1=no agreement to 5=total agreement. Results: 329 participated and answered the questionnaire; 50% were emergency physicians, 40% paramedics, 9% flight nurses, and 1% pilots. Participants agreed that the course taught competencies that were useful for their clinical practice. However, confidence to apply Extra Corporeal Membrane Oxygenation or Intra-Aortic Balloon Pump skills was significantly lower compared to other emergency competencies. Instructors were rated as experienced, engaged and motivated, as well as responsive to course participants. Conclusions: This simulation-based medical education program, with the goal to increase patient’s safety and outcome,was launched successfully. Participants especially valued the time to reflect on clinical performance as well as on crew interaction and ways to apply human factors to improve their team performance and task management

Roads, forestry, and wolves interact to drive moose browsing behavior in Scandinavia

As wild ungulate densities increase across Europe and North America, plant-herbivore interactions are increasingly important from ecological and economic perspectives. These interactions are particularly significant where agriculture and forestry occur and where intensive grazing and browsing by wild ungulates can result in economic losses to growing crops and trees. We studied plant-herbivore interactions in a moose (Alces alces)-dominant system where forestry is a primary economy, the primary and secondary road networks are extensive, and wolves (Canis lupus) are recolonizing. Wolves and humans use low-traffic, secondary roads, yet roadsides provide high-quality and quantity browse for moose. Foraging theory predicts that moose will respond to riskier landscapes by selecting habitats that reduce predation risk, sacrificing feeding time or food quality. As food becomes limiting, however, animals will accept higher predation risk in search of food. We predicted that road avoidance behavior would be strongest within wolf territories. In areas without wolves, moose should select roadsides for their high forage availability. To test these predictions, we measured moose browsing and counted pellet groups as a proxy for habitat use each spring in Norway and Sweden between 2008 and 2018, in areas with and without wolves and at different distances from primary and secondary roads. We used generalized linear mixed models to evaluate drivers of the probability of browsing occurrence and browsing pressure. We found that browsing occurrence increased closer to secondary roads but decreased closer to primary roads. We also found browsing patterns to vary among tree species. For Scots pine (Pinus sylvestris), the browsing occurrence was two times higher in young forests relative to non-young forests and decreased further from secondary roads. Wolf territory presence and probability had neutral or positive effect on browsing occurrence and pressure for all species. However, wolf territory presence had negative effects on browsing occurrence and pressure when interacting with secondary roads, young forest, or snow cover. We showed that roads can influence browsing patterns in Norway and Sweden. However, further research is needed, particularly in the face of continued infrastructure development in Scandinavia

Long-Term Outcome in a Phase II Study of Regional Hyperthermia Added to Preoperative Radiochemotherapy in Locally Advanced and Recurrent Rectal Adenocarcinomas

Hyperthermia was added to standard preoperative chemoradiation for rectal adenocarcinomas in a phase II study. Patients with T3-4 N0-2 M0 rectal cancer or local recurrences were included. Radiation dose was 54 Gy combined with capecitabine 825 mg/m2 × 2 daily and once weekly oxaliplatin 55 mg/m2. Regional hyperthermia aimed at 41.5–42.5 °C for 60 min combined with oxaliplatin infusion. Radical surgery with total or extended TME technique, was scheduled at 6–8 weeks after radiation. From April 2003 to April 2008, a total of 49 eligible patients were recruited. Median number of hyperthermia sessions were 5.4. A total of 47 out of 49 patients (96%) had the scheduled surgery, which was clinically radical in 44 patients. Complete tumour regression occurred in 29.8% of the patients who also exhibited statistically significantly better RFS and CSS. Rate of local recurrence alone at 10 years was 9.1%, distant metastases alone occurred in 25.6%, including local recurrences 40.4%. RFS for all patients was 54.8% after 5 years and CSS was 73.5%. Patients with T50 temperatures in tumours above median 39.9 °C had better RFS, 66.7% vs. 31.3%, p = 0.047, indicating a role of hyperthermia. Toxicity was acceptable.publishedVersio

Development of an IS change reason - IS change type combination matrix

Firms change their information systems (IS) for various reasons, ranging from compliance with government regulations to the development of new capabilities. When making these changes a firm can choose between four different IS change types: IS introduction, IS extension, IS replacement, and IS merger. This paper proposes that change reasons and change types are interrelated, and that certain reason-type combinations are more likely than others to result in a successful IS change. To identify these combinations, an IS change reason–IS change type matrix is developed. While the matrix is created from prior IS research, we conducted a focus group study of IS professionals to further explore and refine the matrix. The findings from the focus group study reveal that some IS change reason–IS change type combinations are more appropriate than others to carry out the IS change project successfully. We also present three examples of IS change projects to illustrate the use and value of the matrix in practice