Polymorphism of k-casein and b-lactoglobuline in simmental cattle in Serbia

The objective of this study was to determine the frequency of the kappa-casein and beta-lactoglobulin genotypes in the Simmental cattle in Serbia and compare it with the frequency according to the Hardy-Weinberg equilibrium law. Blood samples were taken from a total of 157 cows of the Simmental breed in Toplica and Rasina districts. Of the 157 cows included in this study, the AA kappa-casein genotype was found in 53 cows, which makes a frequency of 33.80%, the AB genotype in 81 cows or 51.60% and the BB genotype in 23 cows or 14.60%. The allelic frequency A was 59.60%, while allele B had a frequency of 40.40%. In regard to the frequency of genotypes and beta-lactoglobulin alleles for the total studied population of cows obtained for AA, AB and BB genotypes for beta-lactoglobulin, was 33.10%, 49.70% and 17.20%, respectively, which means that 52 animals had genotype AA, 78 genotype AB and 27 genotype BB. The frequency of alleles A and B resulting from the incidence of genotypes was 58.00% for allele A and 42.00% for allele B. The specified frequencies for both protein fractions statistically differed significantly from the frequency according to the Hardy-Weinberg equilibrium law, which confirmed the absence of equilibrium in the examined population

The Effect of Raw Soybeans in Mixtures for Laying Hens on Egg Quality and Egg Shell Quality

The study was conducted on hens at the age of 49 - 57 weeks to identify opportunities for replacing, with raw grain, thermally processed soybean Lana variety with a reduced level and Lydia variety with a standard level of trypsin inhibitor. The effect of using different levels of participation of both varieties of raw soybean in mixtures for layers on egg quality and eggshell quality was examined. The research was conducted according to the principle of two-factorial experiment (2 varieties x 4 levels of participation of raw soybeans). The average values of properties of the external and internal egg quality were under statistically significant influence of the level of participation of raw soybean in the mixture. Share of 8 % of raw soybeans of both varieties in diets for layers influenced significantly lower eggshape index, albumen height and Haugh's unit in relation to the mixture with a lower level of participation of raw soybeans. Yolk color and eggshell firmness were not significantly influenced by the studied factors

Probiotic features of two oral lactobacillus isolates

In this study, we checked lactobacilli strains of human origin for their potential as probiotic. Samples were collected from oral mucosa of 16 healthy individuals, out of which twenty isolates were obtained and two of them were selected and identified as Lactobacillus plantarum (G1) and L. casei (G3). Both isolates exhibited antagonistic action towards pathogenic microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella abony, and Clostridium sporogenes, but not on the growth of Candida albicans. The bacteriocin activity against Staphylococcus aureus ATCC 6358-P was shown only by L. plantarum G1. Moreover, the isolates G1 and G3 showed good viability in the acid gastric environment and in the gut environment containing bovine bile salts. The viability of G1 and G3 isolates in the gastrointestinal tract, and the adhesion to the intestinal mucosa were also confirmed in vivo. The biochemical tests of blood samples revealed lower levels of serum triglycerides and cholesterol, as well as reduced activity of alkaline phosphatase in all lactobacilli-treated Wistar rats, compared to control ones. No toxicity for NMRI Ham mice was observed. According to our experimental results, these findings imply that L. plantarum G1 and L. casei G3 could be characterized as potential probiotics

Efekat upotrebe sirove soje u smešama za ishranu kokoši nosilja na proizvodne rezultate i relativnu masu pankreasa

The study was conducted on Isa Brown hybrid hens at the age of 49-57 weeks. The effect of using different levels of share of raw soybean of two varieties in mixtures for feeding hens on egg production, body weight, food consumption, the occurrence of defective eggs, mortality and the relative weight of the pancreas was studied. The possibility of replacing the heat-treated soybean grains, varieties Lana, with reduced trypsin inhibitor (TI) and Lydia with a standard level of TI, with raw soybean grains was examined. The research was conducted on the principle of two factorial experiment 2 x 4 (2 varieties x 4 levels of share of raw grain in the mixture) with a total of 8 diet treatments and 4 replicates per each treatment. In the first 5 weeks of the study, the differences in the number of eggs produced under the influence of tested factors were not significant. Under the influence of soybean varieties, the level of share of raw soybean and interaction of the studied factors showed significant differences (p lt 0.01) after 53 week of age. The use of soy with lower TI in the diet for laying hens resulted in a significantly greater capacity compared to standard variety. The share of raw soybean grains of 8 % in the mixtures significantly reduced the number of eggs laid. The differences in body weights, food consumption, occurrence of defective eggs and the relative weight of the pancreas were not significantly influenced by the studied factors or by their interaction effect.Cilj ovih istraživanja je bio da se ispitaju mogućnosti zamene termički obrađenog sojinog zrna, sorte Lana sa smanjenim sadržajem tripsin inhibitora (TI) i sorte Lidija sa standardnim nivoom TI, sirovim zrnom. Ispitan je efekat korišćenja različitog nivoa učešća sirove soje obe sorte u smešama za ishranu kokoši nosilja hibrida Isa Brown na proizvodnju jaja, telesne mase, konzumaciju hrane, pojavu defektnih jaja, mortalitet i relativnu masu pankreasa. Istraživanje je izvedeno po principu dvofaktorijalnog ogleda 2 x 4 (2 sorte soje x 4 nivoa učešća sirovog zrna u smeši) sa ukupno 8 tretmana ishrane i 4 ponavljanja po tretmanu. U prvih 5 nedelja ispitivanja razlike u broju ukupno snešenih jaja pod uticajem ispitivanih faktora nisu bile značajne. Pod uticajem sorte soje, nivoa učešća sirovog sojinog zrna i interakcije ispitivanih faktora utvrđene su značajne razlike (p lt 0,01) nakon 53. nedelje uzrasta. Korišćenje soje sa nižim nivoom TI u ishrani nosilja uticalo je na značajno bolju nosivost u odnosu na standardnu sortu soje. Sa učešćem sirovog sojinog zrna od 8 % u smešama značajno se smanjio broj ukupno snešenih jaja. Razlike u ostvarenim telesnim masama, konzumaciji hrane, pojavi defektnih jaja i relativnoj masi pankreasa koje su se javile nisu bile pod značajnim uticajem ispitivanih faktora kao ni pod uticajem njihovog interakcijskog dejstva

Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns

The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on 16S rRNA gene sequencing. In order to evaluate its probiotic properties in vitro tests were performed: the survival in simulated gastrointestinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tolerance and bacterial translocation in vivo, as well as hemolytic activity in vitro. The isolate G-4, identified as Lactobacillus fermentum, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL(-1) units, respectively), moderate adhesion to hexadecane (39 +/- 2.1 %), and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serotype Abony and Clostridium sporogenes, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed

Encouraging Entrepreneurship: Microfinance, Knowledge Support, and the Costs of Operating in Institutional Voids

This study focuses on the supplemented strategies of microfinance institutions (MFIs), in which the MFI offers nonfinancial services, such as entrepreneurship related knowledge, in addition to financial services to impoverished borrowers at the bottom of the pyramid (BoP). We examine two contextual factors–foreign direct investment (FDI) and loan defaults–to better understand the relationship between providing knowledge support to encourage entrepreneurship and costs of operating at the BoP for MFIs. In contexts where FDI is low and loan defaults are high, providing knowledge support to encourage entrepreneurship aggravates the MFI\u27s costs of operating at the BoP. However, in contexts where FDI is high and loan defaults are low, providing knowledge support to encourage entrepreneurship among impoverished borrowers does not aggravate the MFI\u27s costs of operating at the BoP. Hence, in emerging markets where governments welcome FDI and curb loan defaults, MFIs can viably support entrepreneurship among the poor

Macrophytes as remediation technology in improving ludas lake sediment

Ludas lake is Pannonian plain Lake settled 4 km from Palic. The lake is characterized by thick sediment layers with high content of phosphorus and nitrogen. These thick layers are the result of accelerated eutrophication and cause lost of biodiversity and disruption of ecosystem. Numerous methods can be used to overcome the problem of accelerated eutrophication (sediment removal, phosphorus inactivation etc.), but those methods have a lot deficiencies. To overcome that, the use of macrophytes to resolve the problem of eutophication becomes more and more popular. The main aim of this paper was to examine the germination possibilities of mustard (Sinapis alba L.), alfalfa (Medicago sativa L.), oat (Avena sativa L.) and lettuce (Lactuca sativa L.) on Ludas's sediment as a substrate. This was used as a pre-screening test for plants suitability for further phytoremediation. The results showed that plant species can germinate and grow in early stages in such a substrate, so they can be recommended for sediment phytoremediation

Intensity Fiber-Optic Sensor for Structural Health Monitoring Calibrated by Impact Tester

In this paper, the use of intrinsic intensity fiber-optic sensors for structural health monitoring was investigated. Polyethylene-based composite samples with glass mat reinforcement and embedded telecommunication fibers were subjected to impact using high-speed puncture impact tester as a calibration device. Transfer function of the sensor was generated by linking the light intensity modulation depth with the impact force and the impact point to fiber distance. Conditions for transfer function validity were presented. To detect any possible dangerous impact, a net of crossed fibers was proposed and the net spacing for the given material was calculated. Appropriate non-damaging calibration procedure was proposed, as well as the algorithm for locating the impact point. The method for calculating the impact force, the projectile momentum change, and the place of impact was verified by consecutive strikes on the previously calibrated sample. The sensor can also provide information on the time of the impact and an alarm signal if the force exceeds the damaging threshold force

Preliminary evaluation of the in vitro release and in vivo absorption in rabbits of the modified-release dosage forms

Objective: The suitability of the rabbit as an animal model for the primary screening and selection of the pilot scale batches during the early stages of the formulation development was studied. Materials and methods: Three modified-release formulations of aminophylline consisted of Carbopol (R) 971P/HPMC K4M (F-I), and HPMC K100M (F-II) or HPMC K4M (F-III) were used. Commercial products were Aminofilin retard 350 mg tablets, Srbolek, Serbia (R-I) and Phyllocontin (R) 350, tablets Purdue Frederic, Canada (R-II). Results: Calculated release rate constants and the f2 values between R-I/F-I (84.1) and R-II/F-III (83.4) indicated similar in vitro release while the coefficient n showed presence of different mechanisms of release from Anomalous transport, Fickian diffusion to Case-II transport. Higher T-max, was found in the rabbits, dosed with F-II (12.00 h), F-III (10.50 h), and R-II (15.00 h) formulation. The highest C-max (9.22 mg/L) was obtained with F-II, similar lower values was seen for F-I and F-III, while commercial products showed the lowest values R-I (5.58 mg/L) and R-II (4.18 mg/L). Higher AUC values were detected for all three formulations (from 115.90 to 204.06 mgh/L) in relation to commercial products (105.33 and 113.25 mgh/L). Discussion and conclusion: The results demonstrated a good correlation of Level A (r(2) = 0.97) for the two formulations (F-I, F-III) and commercial product (R-I) indicates that there is a reasonable assumption that the rabbit might be use as a model for the preliminary comparison of scale up formulations in the early stages of the product development