Optimisation of a road construction using 3D visualisation

The main purpose of this work presented in this paper, is the possibility to optimisea road construction using visualisation in 3D, in a scale 1:1. With virtual realitytechnology, we can visualise the whole road and the environment around. Therefore thepossibility to control the position of traffic signals, SOS telephone cabs, noise-protectionroad walls, safety exits, tunnels height or eventual bridges during the virtual visit of theroad. In the same time, we can control the visibility of the road by driver, from start till theend of the road. Doing this, we can help architects, technicians and civil engineeringgroup to modify their plans before they start to construct the real road.In this sense, we can contribute to make the work properly from the beginning,instead of doing several interventions to modify the road that are usually done once theroad is over. Of course, the cost of such interventions is very high and in the same timethe traffic is stopped.Concretly, we realised the A5 highway application. Besides the modelisation withtextures and visualisation of the road, we added the interactivity in real time through adriver of a virtual car. This application run very well, presenting a high degree of virtualreality

Simultaneous multi-band detection of Low Surface Brightness galaxies with Markovian modelling

We present an algorithm for the detection of Low Surface Brightness (LSB) galaxies in images, called MARSIAA (MARkovian Software for Image Analysis in Astronomy), which is based on multi-scale Markovian modeling. MARSIAA can be applied simultaneously to different bands. It segments an image into a user-defined number of classes, according to their surface brightness and surroundings - typically, one or two classes contain the LSB structures. We have developed an algorithm, called DetectLSB, which allows the efficient identification of LSB galaxies from among the candidate sources selected by MARSIAA. To assess the robustness of our method, the method was applied to a set of 18 B and I band images (covering 1.3 square degrees in total) of the Virgo cluster. To further assess the completeness of the results of our method, both MARSIAA, SExtractor, and DetectLSB were applied to search for (i) mock Virgo LSB galaxies inserted into a set of deep Next Generation Virgo Survey (NGVS) gri-band subimages and (ii) Virgo LSB galaxies identified by eye in a full set of NGVS square degree gri images. MARSIAA/DetectLSB recovered ~20% more mock LSB galaxies and ~40% more LSB galaxies identified by eye than SExtractor/DetectLSB. With a 90% fraction of false positives from an entirely unsupervised pipeline, a completeness of 90% is reached for sources with r_e > 3" at a mean surface brightness level of mu_g=27.7 mag/arcsec^2 and a central surface brightness of mu^0 g=26.7 mag/arcsec^2. About 10% of the false positives are artifacts, the rest being background galaxies. We have found our method to be complementary to the application of matched filters and an optimized use of SExtractor, and to have the following advantages: it is scale-free, can be applied simultaneously to several bands, and is well adapted for crowded regions on the sky.Comment: 39 pages, 18 figures, accepted for publication in A

Color display for multiwavelength astronomical images

This paper proposes a new approach for the color display of multispectral/hyperspectral images. The color representation of such data becomes problematic when the number of bands is higher than three, i.e. the basic RGB (Red, Green, Blue) representation is not straightforward. Here we employ a technique that uses a segmentation map, like an a priori information, and then compute a Factorial Discriminant Analysis (Fischer analysis) in order to allow, at best, a distribution of the information in the color space HSV (Hue, Saturation, Value). The information collected from the segmentation map (where each pixel is associated with class) has been shown to be advantages in the representation of the images through the results obtained on increasing size image collections in the framework of astronomical images. This method can easily be applied to other domains such as polarimetric or remote sensing imagery.Cet article propose une nouvelle méthode de représentation et de visualisation en couleur d'images multispectrales ou hyperspectrales. Le problème de la visualisation de telles données est en effet problématique dès que le nombre de bandes spectrales est supérieur à trois, i.e., la représentation triviale RVB (Rouge, Vert, Bleu) n'est plus directe. Le principe consiste ici à utiliser une carte de segmentation préalablement obtenue, a priori, et à réaliser une analyse factorielle discriminante permettant de distribuer au mieux l'information dans l'espace des couleurs TSL (Teinte, Saturation, Luminance). L'information apportée par la carte de segmentation (chaque site est associé à une classe) peut se révéler judicieuse comme le montrent les résultats obtenus sur des lots d'images de tailles croissantes dans le cadre de l'imagerie astronomique. Cette méthode est générale et s'applique également à d'autres domaines manipulant des images multicomposantes ou multivariées comme en télédétection ou en imagerie polarimétrique

Reliability characteristics of microfabricated Rb mini-lamps for optical pumping in miniature atomic clocks and magnetometers

With the rising need for microfabricated chip-scale atomic clocks to enable high precision timekeeping in portable applications, there has been active interest in developing miniature (<few cm3), chip-scale alkali vapor lamps, since vapor plasma discharge sources are currently the standard for optical pumping in double-resonance clocks. We reported in 2012 a first microfabricated chip-scale Rubidium dielectric barrier discharge lamp. The device’s preliminary results indicated its high potential for optical pumping applications and wafer-scale batch fabrication. The chip-scale plasma light sources were observed to be robust with no obvious performance change after thousands of plasma ignitions, and with no electrode erosion from plasma discharges since the electrodes are external. However, as atomic clocks have strict lamp performance requirements including less than 0.1% sub-second optical power fluctuations, power consumption less than 20 mW and a device lifetime of at least several years, it is important to understand the long-term reliability of these Rb planar mini-lamps, and identify the operating conditions where these devices can be most reliable and stable. In this paper, we report on the reliability of such microfabricated lamps including a continuous several month run of the lamp where the optical power, electrical power consumption and temperature stability were continuously monitored. We also report on the effects of temperature, rf-power and the lamp-drive parasitics on the optical power stability and discuss steps that could be taken to further improve the device’s performance and reliability

CPT spectroscopy on low-temperature sealed MEMS rubidium vapour cells

In recent years there has been a strong effort to reduce the size and power consumption of vapour cell atomic clocks [1,2]. The progress in this direction is driven by several factors such as the use low power laser diodes (VCSEL), Coherent Population Trapping resonances (CPT), and micro-fabricated (MEMS) alkali-vapour cells. Here the micro-fabrication of vapour cells has proven a challenging task. All results reported on this task use anodic bonding at high-temperatures (>300°C) to seal the cell [3]. However, the low melting point and high vapour pressure of the alkali-metal combined with long bonding-times (>1hour) complicate this process. We have recently developed a low temperature (~150°C) sealing technique with fast process time (<1min) based on soldering [4]. We report here on the measurement of 85Rb σ+ CPT resonance in low temperature sealed MEMS-fabricated vapour cells containing natural rubidium and buffer gas. The resonance is recorded on the rubidium D1-line (795nm) using a circular polarized and current-modulated VCSEL. We record the resonance shift, linewidth and amplitude as function of several experimental parameters such as light intensity, cell-temperature, and buffer gas pressure- and mixture. In addition we perform noise measurements on the resonance signal to characterize the cell for clock-applications. Preliminary results show a contrast of 1.7% and linewidth of 900Hz for a 4mm long cell with 70mbar of nitrogen buffer gas. Finally we present and characterize two problems related to the application of 85Rb resonance in clock-applications. First, the low modulation frequency of the VCSEL (1.5GHz) leads to a strong asymmetry in the first order sideband spectrum due to the combined effect of AM- and FM modulation. Second, the buffer gas broadening of the absorption spectrum combined with the small separation between VCSEL carrier and sideband reduces the CPT contrast due to off-resonant absorption. We demonstrate that the impact of both these effects can be reduced by modulating the VCSEL at 3GHz and probing the CPT resonance with the carrier and first order sideband. We acknowledge support from the European Space Agency ESA (ESTEC contract number 20794/07/NL/GLC), the Conference Universitaire Suisse CUS (project CIMENT), the Swiss Space Office SSO, and SpectraTime SA (Neuchâtel, Switzerland)

Beneficial insects in agricultural landscapes: faunal, ecological and functional contributions of recent studies in the canton of Geneva (Switzerland)

Description of the subject. In the context of biodiversity maintenance in agricultural environments and of risk reduction related to the use of phytosanitary products, it seems essential to improve our knowledge of the beneficial insects that develop in these ecosystems.Objectives. This article reviews the state of knowledge regarding insect auxiliaries and highlights the main faunal, ecological and functional contributions from recent studies carried out in an agricultural context in Geneva.Method. Data were collected from 2014 to 2019 on several families of predatory and pollinating auxiliaries in various agricultural contexts (extensive grasslands, vineyards, orchards, rapeseed crops, vegetable crops) using Barber, Malaise and emergence traps.Results. 48,921 individual records were collected belonging to 477 species of the main families of auxiliaries. Of these, four species were observed for the first time in Switzerland and 50 in the canton of Geneva. 357 (+/- 75%) of the species had a predatory stage and 103 (+/- 22%) could be regarded as valuable crop auxiliaries. The use of emergence traps made it possible to attest that at least a third of the species, including some rare or threatened species, are able to successfully carry out their life cycles within agricultural environments or to use them as overwintering sites.Conclusions. This review increases our understanding of the composition of the biodiversity of agricultural environments, which constitutes a significant component of biodiversity in Switzerland and in neighboring countries. It also highlights the role of semi-natural habitats as extensive grasslands or inter-rows of vineyards in supporting the development of many rare and sometimes threatened species

Protection of pancreatic beta-cells by high density lipoproteins

SUMMARYThe incidence of type 2 diabetes (T2D) is increasing worldwide and is linked to the enhancement of obesity. The principal cause of T2D development is insulin resistance, which lead to the increase of insulin production by the pancreatic beta-cells. In a pathological environment, namely dyslipidaemia, hyperglycaemia and inflammation, beta-cell compensation will fail in more vulnerable cells and diabetes will occur. High Density Lipoproteins (HDLs), commonly named "good cholesterol" are known to be atheroprotective. Low levels of HDLs are associated with increased prevalence of cardiovascular disease but are also an independent risk factor for the development of T2D. HDLs were demonstrated to protect pancreatic beta-cells against several stresses. However the molecular mechanisms of the protection are unknown and the objectives of this work were to try to elucidate the way how HDLs protect. The first approach was a broad screening of genes regulated by the stress and HDLs. A microarray analysis was performed on beta-cells stressed by serum deprivation and rescued by HDLs. Among the genes regulated, we focused on 4E-BP1, a cap-dependent translational inhibitor. In addition, HDLs were also found to protect against several other stresses.Endoplasmic reticulum (ER) stress is a mechanism that may play a role in the onset of T2D. The unfolded protein response (UPR) is a physiological process that aims at maintaining ER homeostasis in conditions where the protein folding and secretion is perturbed. Specific signalling pathways are involved in the increase of folding, export and degradation capacity of the ER. However, in case where the stress is prolonged, this mechanism turns to be pathological, by inducing cell death effector pathways, leading to beta-cell apoptosis. In our study, we discovered that HDLs were protective against ER stress induced by drugs and physiological stresses such as saturated free fatty acids. HDLs protected beta-cells by promoting ER homeostasis via the improvement of the folding and trafficking od proteins from the ER to the Golgi apparatus.Altogether our results suggest that HDLs are important for beta-cell function and survival, by protecting them from several stresses and acting on ER homeostasis. This suggests that attempt in keeping normal HDLs levels or function in patients is crucial to lessen the development of T2D.RÉSUMÉL'incidence du diabète de type 2 est en constante augmentation et est fortement liée à l'accroissement du taux d'obésité. La cause principale du diabète de type 2 est la résistance à l'insuline, qui entraîne une surproduction d'insuline par les cellules bêta pancréatiques. Dans un environnement pathologique associé à l'obésité (dyslipidémie, hyperglycémie et inflammation), les cellules bêta les plus vulnérables ne sont plus capables de compenser en augmentant leur production d'insuline, dysfonctionnent, ce qui conduit à leur mort par apoptose. Les lipoprotéines de hautes densités (HDLs), communément appelées (( bon cholestérol », sont connues pour leurs propriétés protectrices contre l'athérosclérose. Des niveaux bas de HDLs sanguins sont associés au risque de développer un diabète de type 2. Les HDLs ont également montré des propriétés protectrices contre divers stresses dans la cellule bêta. Cependant, les mécanismes de protection restent encore inconnus et l'objectif de ce travail a été d'investiguer les mécanismes moléculaires de protection des HDLs. La première approche choisie a été une étude du profil d'expression génique par puce à ADN afin d'identifier les gènes régulés par le stress et les HDLs. Parmi les gènes régulés, notre intérêt s'est porté sur 4E-BP1, un inhibiteur de la traduction coiffe- dépendante, dont l'induction par le stress était corrélée avec une augmentation de l'apoptose. Suite à cette étude, les HDLs ont également montrés un rôle protecteur contre d'autres stresses. Il s'agit particulièrement du stress du réticulum endoplasmique (RE), qui est un mécanisme qui semble jouer un rôle clé dans le développement du diabète. L'UPR (« Unfolded Protein Response ») est un processus physiologique tendant à maintenir l'homéostasie du réticulum endoplasmique, organelle prépondérante pour la fonction des cellules sécrétrices, notamment lorsqu'elle est soumise à des conditions extrêmes telles que des perturbations de la conformation tertiaire des protéines ou de la sécrétion. Dans ces cas, des voies de signalisation moléculaires sont activées, ce qui mène à l'exportation des protéines mal repliées, à leur dégradation et à l'augmentation de l'expression de chaperonnes capables d'améliorer le repliement des protéines mal formées. Toutefois, en cas de stress persistant, ce mécanisme de protection s'avère être pathologique. En induisant des voies de signalisation effectrices de l'apoptose, il conduit finalement au développement du diabète. Dans cette étude, nous avons démontré que les HDLs étaient capables de protéger la cellule bêta contre le stress du RE induits par des inhibiteurs (thapsigargine, tunicamycine) ou des stresses physiologiques tels que les acides gras libres. Les HDLs ont la capacité d'améliorer l'homéostasie du RE, notamment en favorisant le repliement et le transfert des protéines du RE à l'appareil de Golgi.En résumé, ces données suggèrent que les HDLs sont bénéfiques pour la survie des cellules bêta soumises à des stresses impliqués dans le développement du diabète, notamment en restaurant l'homéostasie du RE. Ces résultats conduisent à soutenir que le maintien des taux de cholestérol joue un rôle important dans la limitation de l'incidence du diabète

Low surface brightness galaxy detection, hyperspectral segmentation within the framework of the virtual observatory

Les progrès technologiques de l'instrumentation astronomique soulèvent des problématiques variées. En particulier, le développement des capteurs multispectraux permet l'acquisition de masses de données porteuses d'une information très riche. Néanmoins, l'interprétation et le traitement de tels volumes de données restent délicats pour la communauté astronomique. Dans le cadre de cette thèse nous proposons une méthode de détection de galaxies à faible brillance de surface basée sur l'utilisation d'une segmentation markovienne par quadarbre. Puis, nous étudions une nouvelle méthode de segmentation de cubes de données hyperspectraux basée sur une approche spectrale puis sur une régularisation spatiale de la carte de segmentation. Enfin, nous proposons deux méthodes de visualisation d'images multibandes, ainsi qu une méthode de segmentation floue par champs de Markov. Ces méthodes sont validées sur des images astronomiques et ont fait l'objet d'une interaction particulièrement riche entre communauté STIC et communauté astronomique.Technological progress in astronomical instrumentation raise various issues. The development of multispectral sensors yields extremely valuable data. Nevertheless interpretation and processing of such images remain tricky for the astronomical community. Within the framework of this thesis we propose a new method for the detection of low surface brightness galaxy based on a quadtree Markovian segmentation. We then introduce a new segmentation method of hyperspectral data cubes based on a spectral discrimination and on a spatial regularization of the segmentation map. We then propose two multispectral images visualization methods and a new fuzzy segmentation method based on Markov fields. These methods are validated on astronomical images and led to a fruitful cooperation between STIC and astronomical community