Využití bankrotního a bonitního modelu při hodnocení výkonnosti podnikatelských subjektů ve strojírenském průmyslu

The diploma thesis focuses on evaluating bankruptcy models and provides appro-priate optimization for engineering companies to minimize bankruptcy risks. The first part includes model construction via neural networks. The model is consecu-tively compared to Z-score and index IN05. Based on the results of the comparison, factors that could highly increase bankruptcy risks will be identified

Evolution of mastigont in Excavata

In recent years we have been discovering a huge diversity of eukaryotic organisms. One of the big super-groups is Excavata. Examination of the organisms from this group is fairly important as far as theoretical and practical reasons are concerned. A significant number of authors believe that there is the root of all eukaryotic organisms in this group. And what is more, a lot of studies prove the point. Within Excavata group we can find the organisms with the most primitive mitochondrial genome which is known. Several other representatives are an important human pathogenes. In the first part of the thesis I focus on the historical review of the creation of the super-group Excavata and I also include a part regarding the theory about Archezoa. The next section deals with the general description of mastigont (basal bodies and cytoskeleton that is associated with them). The last part presents mastigont descriptions of some derived representatives of Excavata. Some of these organisms may be free-living and some live inside other organisms

PHILHARMONIC HRADEC KRALOVE AND ITS TRUMPET SECTION

The author of this bachelor thesis is Petr Táborský, student of the third grade of the Department of Wind Instruments on the Music Faculty of The Academy of Performing Arts in Prague. The subject of this bachelor thesis is The Hradec Králové Philharmonic Orchestra ("HKPO") and its trumpet section. The main goal of the thesis was to analyze the history of the HKPO up to the present, biography of current and former members of the trumpet section of the HKPO and biography of the most significant chief conductors of the HKPO. The bachelor thesis was prepared under the supervision of Prof. Vladimír Rejlek

Morphological and molecular characterization of anaerobic members of the genus Andalucia (Excavata: Jakobida)

Eukaryotic organisms are divided into about 30 groups today, yet there still reamain many groups whose classification in the eukaryotic tree we are not sure about. This study focused on jacobids, a evolutionary important protist group from the Excavata taxa. Together with Heterolobosea, Euglenozoa and Tsukubamonadida, jacobids constitue a Discoba subgroup of Excavata taxa. Jacobids are significant mainly for their mitochondrial genome, which structurally resembles the original α-proteobacterial genome of the free-living ancestor of mitochondria. Some jacobids have their mitochondrial genome simplified, making them potential candidates for a model group used to study the mitochondrial evolution. However, too few members of jacobid group have been identified so far and the exact phylogeny of the group must be thoroughly understood for the detailed study of mitochondria evolution. This study focuses on broadening the taxonomic coverage of the jacobid excavate protists Andalucia. Twenty new strains of Andalucia have been established into stable culture, their morphology was assessed and SSU rDNA sequenced. Based on the phylogenetic analysis, Andalucia incarcarata species divides into three morphologically almost indistinguishable lines representing three species. Further, this study characterized a..

Influence of Solvent Polarity and DNA-Binding on Spectral Properties of Quaternary Benzo[<i>c</i>]phenanthridine Alkaloids

<div><p>Quaternary benzo[<i>c</i>]phenanthridine alkaloids are secondary metabolites of the plant families <i>Papaveraceae</i>, <i>Rutaceae</i>, and <i>Ranunculaceae</i> with anti-inflammatory, antifungal, antimicrobial and anticancer activities. Their spectral changes induced by the environment could be used to understand their interaction with biomolecules as well as for analytical purposes. Spectral shifts, quantum yield and changes in lifetime are presented for the free form of alkaloids in solvents of different polarity and for alkaloids bound to DNA. Quantum yields range from 0.098 to 0.345 for the alkanolamine form and are below 0.033 for the iminium form. Rise of fluorescence lifetimes (from 2–5 ns to 3–10 ns) and fluorescence intensity are observed after binding of the iminium form to the DNA for most studied alkaloids. The alkanolamine form does not bind to DNA. Acid-base equilibrium constant of macarpine is determined to be 8.2–8.3. Macarpine is found to have the highest increase of fluorescence upon DNA binding, even under unfavourable pH conditions. This is probably a result of its unique methoxy substitution at C₁₂ a characteristic not shared with other studied alkaloids. Association constant for macarpine-DNA interaction is 700000 M^-1.</p></div

Acid-base properties of 3 μM macarpine.

<p>Dependence of absorbance at 495 nm (black) and fluorescence at 450 nm (red) on pH, n = 3. Mean values ± SD and fits to Eq (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0129925#pone.0129925.e004" target="_blank">4</a>) are shown. Inset: Absorption (black) and emission (red) spectra of iminium (—) and alkanolamine (…) form. Ordinates are the same as for bigger figure.</p

Absorbance and fluorescence spectra of QBAs in absence and presence of ctDNA.

<p>QBAs (3 μM in 20mM acetate buffer, 200 mM NaCl, 2mM EDTA, pH 5) in absence (—) and presence (…) of ctDNA (DNA base pair-to-drug ratio 15.9:1). a-f–absorption spectra, g-l–emission spectra; a, g–sanguinarine, b, h–chelerythrine, c, i–chelirubine, d, j–sanguilutine, e, k–sanguirubine, f, l–macarpine. Note that intensities are in arbitrary units due to conversion to wavenumber scale using Eq (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0129925#pone.0129925.e001" target="_blank">1</a>).</p

Lippert–Mataga plot of QBAs.

<p>Black–macarpine, red–sanguilutine, blue–chelirubine, green–sanguinarine, gold–sanguirubine, violet–chelerythrine; 1 –benzene, 2 –diethyl ether, 3 –octanol, 4 –ethanol, 5 –methanol, 6–0.01M borate buffer, pH 9.45. Samples in borate buffer (dashed box) excluded from fitting.</p