5 research outputs found
Economic Ideas and Institutional Change: Evidence from Soviet Economic Discourse 1987-1991
Multiplex Biosensing Based on Highly Sensitive Magnetic Nanolabel Quantification: Rapid Detection of Botulinum Neurotoxins A, B, and E in Liquids
We present a multiplex
quantitative lateral flow (LF) assay for
simultaneous on-site detection of botulinum neurotoxin (BoNT) types
A, B, and E in complex matrixes, which is innovative by virtually
no sacrifice in performance while transition from the single-plex
assays and by characteristics on the level of laboratory quantitative
methods. The novel approach to easy multiplexing is realized via joining
an on-demand set of single-plex LF strips, which employ magnetic nanolabels,
into a miniature cylinder cartridge that mimics LF strip during all
assay stages. The cartridge is read out by an original portable multichannel
reader based on the magnetic particle quantification technique. The
developed reader offers the unmatched 60 zmol detection limit and
7-order linear dynamic range for volumetric registration of magnetic
labels inside a cartridge of several millimeters in diameter regardless
of its optical transparency. Each of the test strips, developed here
as building blocks for the multiplex assay, can be used “as
is” for autonomous quantitative single-plex detection with
the same measuring setup, exhibiting the limits of detection (LOD)
of 0.22, 0.11, and 0.32 ng/mL for BoNT-A, -B, and -E, respectively.
The proposed multiplex assay has demonstrated the remarkably similar
LOD values of 0.20, 0.12, 0.35 ng/mL under the same conditions. The
multiplex assay performance was successfully validated by BoNT detection
in milk and apple and orange juices. The developed methods can be
extended to other proteins and used for rapid multianalyte tests for
point-of-care <i>in vitro</i> diagnostics, food analysis,
biosafety and environmental monitoring, forensics, and security, etc