Modular System of Casino

Práce je zaměřena na modulární vývoj hazardního herního systému. V systému se nachází jeden hlavní modul obsluhující databázi hráčů a uživatelských účtů. Každá hra v tomto systému je dvojice modulů; herní server, který realizuje hru, a klientská aplikace, která nabízí uživatelské rozhraní této hře. Veškerá síťová komunikace probíhá pomocí mojí navržené multiplatformní síťové knihovny TCP_Network, pomocí navržených protokolů pro každý server.This thesis is pointed to a modular development of hazard game system. There is one main module in this system, it services gamers database and user accounts. Every game in this system is module pair; first it is game server, it realizes the game, and second client application, it has user interface for this game. Every network communications are realized using my own multi platform network library TCP_Network, and using network protocol for every server.

Library for Operations over Finite Automata

Tato práce se zabývá dvěma základními operacemi nad konečnými automaty. Determinizací nedeterministických konečných automatů a minimalizací deterministických konečných automatů. Pro obě tyto operace jsem navrhoval sekvenční algoritmy, které jsou paralelizovatelné. Zabývám se hledáním zrychlení především pomocí SSE instrukcí nebo pomocí knihovny openMP. Trendem dnešní doby je především zvyšování počtu procesorů, proto budu navrhovat paralelní algoritmy pro více procesorů. Při hledání optimálního řešení budu zkoumat další možnosti, jak dosáhnout zrychlení, např. efektivním uložením datových struktur v paměti.This work deals with two basic operations over finite automata. Determination of nondeterministic finite automata and minimization of deterministic finite automata. For these two operations I proposed sequential algorithms that are parallelizable. I deal mainly with finding the speedup of SSE instructions, or use the OpenMP library. The trend today is mainly in increasing the number of processors, so I propose parallel algorithms for multiple processors. When searching for the optimal solution, I will be to examine other ways to achieve speedup, for example efficient saving of the data structures in memory.

Effects of Radiation Therapy on Neural Stem Cells

Brain and nervous system cancers in children represent the second most common neoplasia after leukemia. Radiotherapy plays a significant role in cancer treatment; however, the use of such therapy is not without devastating side effects. The impact of radiation-induced damage to the brain is multifactorial, but the damage to neural stem cell populations seems to play a key role. The brain contains pools of regenerative neural stem cells that reside in specialized neurogenic niches and can generate new neurons. In this review, we describe the advances in radiotherapy techniques that protect neural stem cell compartments, and subsequently limit and prevent the occurrence and development of side effects. We also summarize the current knowledge about neural stem cells and the molecular mechanisms underlying changes in neural stem cell niches after brain radiotherapy. Strategies used to minimize radiation-related damages, as well as new challenges in the treatment of brain tumors are also discussed

Calcium Ionophore, Calcimycin, Kills Leishmania Promastigotes by Activating Parasite Nitric Oxide Synthase

Leishmaniasis is an infectious disease caused by protozoan parasites of the genus Leishmania. There is no vaccine against human leishmaniasis and the treatment of the disease would benefit from a broader spectrum and a higher efficacy of leishmanicidal compounds. We analyzed the leishmanicidal activity and the mechanism of action of the calcium ionophore, calcimycin. L. major promastigotes were coincubated with calcimycin and the viability of the cells was assessed using resazurin assay. Calcimycin displayed dose-dependent effect with IC50 = 0.16 μM. Analysis of propidium iodide/LDS-751 stained promastigotes revealed that lower concentrations of calcimycin had cytostatic effect and higher concentrations had cytotoxic effect. To establish the mechanism of action of calcimycin, which is known to stimulate activity of mammalian constitutive nitric oxide synthase (NOS), we coincubated L. major promastigotes with calcimycin and selective NOS inhibitors ARL-17477 or L-NNA. Addition of these inhibitors substantially decreased the toxicity of calcimycin to Leishmania promastigotes. In doing so, we demonstrated for the first time that calcimycin has a direct leishmanicidal effect on L. major promastigotes. Also, we showed that Leishmania constitutive Ca2+/calmodulin-dependent nitric oxide synthase is involved in the parasite cell death. These data suggest activation of Leishmania nitric oxide synthase as a new therapeutic approach