161 research outputs found
Discovery of a low-eccentricity, high-inclination Kuiper belt object at 58 AU
We report the discovery of the first trans-neptunian object, designated 2004
XR190, with a nearly-cirular orbit beyond the 2:1 mean-motion resonance.
Fitting an orbit to 23 astrometric observations spread out over 12 months
yields an orbit of a=57.2\pm0.4, e=0.08\pm0.04, and i=46.6 deg. All viable
orbits have perihelia distances q>49 AU. The very high orbital inclination of
this extended scattered disk object might be explained by several models, but
its existence again points to a large as-yet undiscovered population of
transneptunian objects with large orbital perihelia and inclination.Comment: 3 figures, submitted to ApJ
Plasmon channels in the electronic relaxation of diamond under high-order harmonics femtosecond irradiation
We used high order harmonics of a femtosecond titanium-doped sapphire system
(pulse duration 25 fs) to realise Ultraviolet Photoelectron Spectroscopy (UPS)
measurements on diamond. The UPS spectra were measured for harmonics in the
range 13 to 27. We also made ab initio calculations of the electronic lifetime
of conduction electrons in the energy range produced in the UPS experiment.
Such calculations show that the lifetime suddenly diminishes when the
conduction electron energy reaches the plasmon energy, whereas the UPS spectra
show evidence in this range of a strong relaxation mechanism with an increased
production of low energy secondary electrons. We propose that in this case the
electronic relaxation proceeds in two steps : excitation of a plasmon by the
high energy electron, the latter decaying into individual electron-hole pairs,
as in the case of metals. This process is observed for the first time in an
insulator and, on account of its high efficiency, should be introduced in the
models of laser breakdown under high intensity
Ionization dynamics in intense pulsed laser radiation. Effects of frequency chirping
Via a non-perturbative method we study the population dynamics and
photoelectron spectra of Cs atoms subject to intense chirped laser pulses, with
gaussian beams. We include above threshold ionization spectral peaks. The
frequency of the laser is near resonance with the 6s-7p transition. Dominant
couplings are included exactly, weaker ones accounted for perturbatively. We
calculate the relevant transition matrix elements, including spin-orbit
coupling. The pulse is taken to be a hyperbolic secant in time and the chirping
a hyperbolic tangent. This choice allows the equations of motions for the
probability amplitudes to be solved analytically as a series expansion in the
variable u=(tanh(pi t/tau)+1)/2, where tau is a measure of the pulse length. We
find that the chirping changes the ionization dynamics and the photoelectron
spectra noticeably, especially for longer pulses of the order of 10^4 a.u. The
peaks shift and change in height, and interference effects between the 7p
levels are enhanced or diminished according to the amount of chirping and its
sign. The integrated ionization probability is not strongly affected.Comment: Accepted by J. Phys. B; 18 pages, 17 figures. Latex, uses
ioplppt.sty, iopl10.sty and psfig.st
Explorando el potencial bioestimulante del alga invasora Rugulopterix okamurae en vid
Trabajo presentado en las IV Jornadas del Grupo de Viticultura de la Sociedad Española de Ciencias Hortícolas, celebradas en Pamplona (España), del 26 al 28 de octubre de 202
Biological response to pre-mineralized starch based scaffolds for bone tissue engineering
It is known that calcium-phosphate (Ca-P) coatings are able not only to improve the bone
bonding behaviour of polymeric materials, but at the same time play a positive role on
enhancing cell adhesion and inducing the differentiation of osteoprogenitor cells. Recently
an innovative biomimetic methodology, in which a sodium silicate gel was used as a
nucleative agent, was proposed as an alternative to the currently available biomimetic
coating methodologies. This methodology is especially adequate for coating biodegradable
porous scaffolds. In the present work we evaluated the influence of the referred to
treatment on the mechanical properties of 50/50 (wt%) blend of corn starch/ethylene-vinyl
alcohol (SEVA-C) based scaffolds. These Ca-P coated scaffolds presented a compressive
modulus of 224.6 ± 20.6 and a compressive strength of 24.2 ± 2.20. Cytotoxicity evaluation
was performed according ISO/EN 10993 part 5 guidelines and showed that the biomimetic
treatment did not have any deleterious effect on L929 cells and did not inhibit cell growth.
Direct contact assays were done by using a cell line of human osteoblast like cells (SaOS-2).
3 × 105 cells were seeded per scaffold and allowed to grow for two weeks at 37 ◦C in a
humidified atmosphere containing 5% CO2. Total protein quantification and scanning
electron microscopy (SEM) observation showed that cells were able to grow in the
pre-mineralized scaffolds. Furthermore cell viability assays (MTS test) also show that cells
remain viable after two weeks in culture. Finally, protein expression studies showed that
after two weeks osteopontin and collagen type I were being expressed by SaOS-2 cells
seeded on the pre-mineralized scaffolds. Moreover, alkaline phosphatase (ALP) activity was
higher in the supernatants collected from the pre-mineralized samples, when compared to
the control samples (non Ca-P coated). This may indicate that a faster mineralization of the
ECM produced on the pre-mineralized samples was occurring. Consequently, biomimetic
pre-mineralization of starch based scaffolds can be a useful route for applying these
materials on bone tissue engineering
The progress of early phase bone healing using porous granules produced from calcium phosphate cement
<p>Abstract</p> <p>Objective</p> <p>Bone grafting is a vital component in many surgical procedures to facilitate the repair of bone defects or fusions. Autologous bone has been the gold standard to date in spite of associated donor-site morbidity and the limited amount of available donor bone. The aim of this study was to investigate the progress of bone regeneration and material degradation of calcium phosphate granules (CPG) produced from a calcium phosphate self-setting cement powder compared to the use of autologous bone grafting in the treatment of "critical size defects" on load-bearing long bones of minipigs.</p> <p>Methods</p> <p>A critical size defect in the tibial metaphysis of 16 mini-pigs was filled either with autologous cancellous graft or with micro- and macroporous carbonated, apatic calcium phosphate granules (CPG) produced from a calcium phosphate self-setting cement powder. After 6 weeks, the specimens were assessed by X-ray and histological evaluation. The amount of new bone formation was analysed histomorphometrically.</p> <p>Results</p> <p>The semi-quantitative analysis of the radiological results showed a complete osseous bridging of the defect in three cases for the autograft group. In the same group five animals showed a beginning, but still incomplete bridging of the defect, whereas in the CPG group just two animals developed this. All other animals of the CPG group showed only a still discontinuous new bone formation. Altogether, radiologically a better osseous bridging was observed in the autograft group compared to the CPG group.</p> <p>Histomorphometrical analysis after six weeks of healing revealed that the area of new bone was significantly greater in the autograft group concerning the central area of the defect zone (p < 0.001) as well as the cortical defect zone (p < 0.002). All defects showed new bone formation, but only in the autograft group defects regenerated entirely</p> <p>Conclusions</p> <p>Within the limits of the present study it could be demonstrated that autologous cancellous grafts lead to a significantly better bone regeneration compared to the application of calcium phosphate granules (CPG) produced from a calcium phosphate self-setting cement powder after 6 weeks. In the early phase of bone-healing, the sole application of CPG appears to be inferior to the autologous cancellous grafts in an <it>in vivo </it>critical size defect on load-bearing long bones of mini-pigs.</p
The Response of Vocal Fold Fibroblasts and Mesenchymal Stromal Cells to Vibration
Illumination of cellular changes caused by mechanical forces present within the laryngeal microenvironment may well guide strategies for tissue engineering the vocal fold lamina propria. The purpose of this study was to compare the response of human vocal fold fibroblasts (hVFF) and bone marrow mesenchymal stem cells (BM-MSC) to vibratory stimulus. In order to study these effects, a bioreactor capable of vibrating two cell seeded substrates was developed. The cell seeded substrates contact each other as a result of the sinusoidal frequency, producing a motion similar to the movement of true vocal folds. Utilizing this bioreactor, hVFF and BM-MSC were subjected to 200 Hz vibration and 20% strain for 8 hours. Immunohistochemistry (Ki-67 and TUNEL) was performed to examine cell proliferation and apoptosis respectively, while semi-quantitative RT-PCR was used to assess extracellular matrix related gene expression. HVFF significantly proliferated (p = 0.011) when subjected to 200 Hz vibration and 20% strain, while BM-MSC did not (p = 1.0). A statistically significant increase in apoptosis of BM-MSC (p = 0.0402) was observed under the experimental conditions; however high cell viability (96%) was maintained. HVFF did not have significantly altered apoptosis (p = 0.7849) when subjected to vibration and strain. Semi-quantitative RT-PCR results show no significant differences in expression levels of collagen I (BM-MSC p = 0.1951, hVFF p = v0.3629), fibronectin (BM-MSC p = 0.1951, hVFF p = 0.2513), and TGF-β1 (BM-MSC p = 0.2534, hVFF p = 0.6029) between vibratory and static conditions in either cell type. Finally, smooth muscle actin mRNA was not present in either vibrated or static samples, indicating that no myofibroblast differentiation occurred for either cell type. Together, these results demonstrate that BM-MSC may be a suitable alternative to hVFF for vocal fold tissue engineering. Further investigation into a larger number of gene markers, protein levels, increased number of donors and vibratory conditions are warranted
Bovine pericardium based non-cross linked collagen matrix for successful root coverage, a clinical study in human
Introduction: The aim of this study was to clinically assess the capacity of a novel bovine pericardium based, non-cross linked collagen matrix in root coverage.
Methods: 62 gingival recessions of Miller class I or II were treated. The matrix was adapted underneath a coronal repositioned split thickness flap. Clinical values were assessed at baseline and after six months.
Results: The mean recession in each patient was 2.2 mm at baseline. 6 Months after surgery 86.7% of the exposed root surfaces were covered. On average 0,3 mm of recession remained. The clinical attachment level changed from 3.5 ± 1.3 mm to 1,8 ( ± 0,7) mm during the observational time period. No statistically significant difference was found in the difference of probing depth. An increase in the width of gingiva was significant. With a baseline value of 1.5 ± 0.9 mm an improvement of 2.4 ± 0.8 mm after six month could be observed. 40 out of 62 recessions were considered a thin biotype at baseline. After 6 months all 62 sites were assessed thick.
Conclusions: The results demonstrate the capacity of the bovine pericardium based non-cross linked collagen matrix for successful root coverage. This material was able to enhance gingival thickness and the width of keratinized gingiva. The percentage of root coverage achieved thereby is comparable to existing techniques. This method might contribute to an increase of patient's comfort and an enhanced aesthetical outcome
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