258 research outputs found
After Revolution and Democratization: Conceptualizing the 18th Brumaire and Regimes of the two Bonapartes.
Le bicentenaire du coup d’État de Bonaparte a donné lieu à une rencontre organisée par Melvin Richter à Hunter College, co-sponsorisée par le German Historical Institute, et qui était aussi la réunion internationale de la Conference for the Study of Political Thought. Elle a réuni un grand nombre de chercheurs, historiens, sociologues, théoriciens et spécialistes de science politique. Les débats ont été centrés sur la nature et le legs des deux empires napoléoniens. La conférence s’organisait..
Architectural photogrammetry and picture processing for acquisition and documentation of a Brazilian town ensemble
Identification and light-dependent translocation of a cone-specific antigen, cone arrestin, recognized by monoclonal antibody 7G6
PURPOSE: To elucidate the antigen recognized by monoclonal antibody (mAb) 7G6, a widely used cone-specific marker. METHODS: 7G6 immunocytochemistry was performed on sections of human, primate, and bovine retina. The antigen was immunoprecipitated from human retinal lysates and purified with protein G. Edman degradation and liquid chromatography of tryptic peptides combined with tandem mass spectrometry (LC-MS/MS) identified the antigen. RESULTS: Sequencing of peptides derived from the immunoprecipitated 7G6 antigen identified it as cone arrestin. The identity was confirmed by Western blot analysis with recombinant human cone arrestin and competition with the antibody in immunocytochemistry. Subcellular localization of cone arrestin in dark-adapted and bleached bovine retinas showed that cone arrestin accumulated in cone outer segments of light-adapted retina but was more concentrated in the inner segments of dark-adapted retina. By expression of truncated human cone arrestin mutants systematically deleting areas divergent from bovine and primate cone arrestins, the epitope of 7G6 was identified as a divergent loop exposed at the surface within the N-domain of cone arrestin. CONCLUSIONS: Several independent methods established that the 7G6 antigen is cone arrestin. The 7G6 epitope is contained in a divergent loop, the sequence of which is conserved in bovine and primates but not other vertebrate species consistent with the specificity of the antibody. The light-dependent translocation of cone arrestin suggests a role in light-dark adaptation of cones. Because of the location of its gene on the X-chromosome, cone arrestin is a candidate gene for X-linked cone dystrophies.Supported by NIH Grant R01EY08123 (WB). Additional support came from the Macular Vision Research Foundation, Research to Prevent Blindness, Inc., and a Center grant from the Foundation Fighting Blindness to the University of Utah. WB is the recipient of a Senior Investigator Award from RPB and a Ralph and Mary Tuck endowment to the Department of Ophthalmology at the University of Utah
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