Impacto economico da questão ambiental no processo decisorio do investimento em mineração

Orientador: Saul B. SuslickTese (doutorado) - Universidade Estadul de Campinas, Instituto de GeocienciasDoutorad

Periodontitis and peri-implantitis tissue levels of Treponema denticola-CTLP and its MMP-8 activating ability

Background and Aims: Chymotrypsin-like-proteinase of Treponema denticola (Td-CTLP) can stimulate the protein expression and activation of matrix metalloproteinase (MMP)-8 (or collagenase-2), a potent tissue destructive enzyme from gingival cells in vitro. The aims of this study were 1) to demonstrate the proMMP-8 (or latent MMP-8) activation by Td-CTLP in vitro and 2) to detect Td-CTLP and MMP-8 protein levels in the tissue samples of peri-implantitis and periodontitis patients. Materials and Methods: proMMP-8 activation by Td-CTLP was analyzed by immunoblots. Tissue specimens were collected from 38 systemically healthy and non-smoking patients; 14 of whom had moderate to severe periodontitis, 10 of whom were suffering from peri-implantitis, and finally 14 of whom showed no sign of periodontal inflammation nor radiological bone decay (control group). The immune-expression levels of MMP-8 and Td-CTLP in the epithelium and the connective tissue were analyzed immunohistochemically. A pixel color-intensity analyze was performed with ImageJ software (version 1.46c; Rasband WS, National Institutes of Health, Bethesda, MD, USA) to obtain a comparable numeral score for each patient's epithelium and connective tissue MMP-8 and Td-CTLP enzyme level. Results: Td-CTLP activated proMMP-8 in vitro by converting the 70 75 kDa proMMP-8 to 65 kDa active MMP-8. Also, lower molecular size 25 50 kDa parts of MMP-8 were formed. There was no statistically significant difference between the study groups in terms of their MMP-8 and Td-CTLP levels in the epithelium or in the connective tissue. Conclusion: Regarding the limits of this study, it can thus be said that the Td-CTLP enzyme can activate the host proMMP-8 enzyme. Tissue protein levels of MMP-8 and Td-CTLP do not seem to be changed in peri-implantitis and in periodontitis.Peer reviewe

Periodontitis and peri-implantitis tissue levels of Treponema denticola-CTLP and its MMP-8 activating ability

Background and Aims: Chymotrypsin-like-proteinase of Treponema denticola (Td-CTLP) can stimulate the protein expression and activation of matrix metalloproteinase (MMP)-8 (or collagenase-2), a potent tissue destructive enzyme from gingival cells in vitro. The aims of this study were 1) to demonstrate the proMMP-8 (or latent MMP-8) activation by Td-CTLP in vitro and 2) to detect Td-CTLP and MMP-8 protein levels in the tissue samples of peri-implantitis and periodontitis patients.Materials and Methods: proMMP-8 activation by Td-CTLP was analyzed by immunoblots. Tissue specimens were collected from 38 systemically healthy and non-smoking patients; 14 of whom had moderate to severe periodontitis, 10 of whom were suffering from peri-implantitis, and finally 14 of whom showed no sign of periodontal inflammation nor radiological bone decay (control group). The immune-expression levels of MMP-8 and Td-CTLP in the epithelium and the connective tissue were analyzed immunohistochemically. A pixel color-intensity analyze was performed with ImageJ software (version 1.46c; Rasband WS, National Institutes of Health, Bethesda, MD, USA) to obtain a comparable numeral score for each patient's epithelium and connective tissue MMP-8 and Td-CTLP enzyme level.Results: Td-CTLP activated proMMP-8 in vitro by converting the 70 75 kDa proMMP-8 to 65 kDa active MMP-8. Also, lower molecular size 25 50 kDa parts of MMP-8 were formed. There was no statistically significant difference between the study groups in terms of their MMP-8 and Td-CTLP levels in the epithelium or in the connective tissue.Conclusion: Regarding the limits of this study, it can thus be said that the Td-CTLP enzyme can activate the host proMMP-8 enzyme. Tissue protein levels of MMP-8 and Td-CTLP do not seem to be changed in peri-implantitis and in periodontitis.</p

Periodontitis and Peri-Implantitis Tissue Levels of Treponema denticola-CTLP and its MMP-8 Activating Ability

Abstract Background and Aims: Chymotrypsin-like-proteinase of Treponema denticola (Td-CTLP) can stimulate the protein expression and activation of matrix metalloproteinase (MMP)-8 (or collagenase-2), a potent tissue destructive enzyme from gingival cells in vitro. The aims of this study were 1) to demonstrate the proMMP-8 (or latent MMP-8) activation by Td-CTLP in vitro and 2) to detect Td-CTLP and MMP-8 protein levels in the tissue samples of peri-implantitis and periodontitis patients. Materials and Methods: proMMP-8 activation by Td-CTLP was analyzed by immunoblots. Tissue specimens were collected from 38 systemically healthy and non-smoking patients; 14 of whom had moderate to severe periodontitis, 10 of whom were suffering from peri-implantitis, and finally 14 of whom showed no sign of periodontal inflammation nor radiological bone decay (control group). The immune-expression levels of MMP-8 and Td-CTLP in the epithelium and the connective tissue were analyzed immunohistochemically. A pixel color-intensity analyze was performed with ImageJ software (version 1.46c; Rasband WS, National Institutes of Health, Bethesda, MD, USA) to obtain a comparable numeral score for each patient’s epithelium and connective tissue MMP-8 and Td-CTLP enzyme level. Results: Td-CTLP activated proMMP-8 in vitro by converting the 70-75 kDa proMMP-8 to 65 kDa active MMP-8. Also, lower molecular size 25-50 kDa parts of MMP-8 were formed. There was no statistically significant difference between the study groups in terms of their MMP-8 and Td-CTLP levels in the epithelium or in the connective tissue. Conclusion: Regarding the limits of this study, it can thus be said that the Td-CTLP enzyme can activate the host proMMP-8 enzyme. Tissue protein levels of MMP-8 and Td-CTLP do not seem to be changed in peri-implantitis and in periodontitis

Isolation and partial purification of L-asparaginase produced by actinobacteria in soil of the cerrado

Submitted by Marlene Santos ([email protected]) on 2016-06-15T20:56:23Z No. of bitstreams: 2 Dissertacão - Petain Jose Ferreira Neto - 2015.pdf: 1935098 bytes, checksum: 58b97940c1a11dda51ddc983a41e4bd9 (MD5) license_rdf: 19874 bytes, checksum: 38cb62ef53e6f513db2fb7e337df6485 (MD5)Approved for entry into archive by Luciana Ferreira ([email protected]) on 2016-06-28T13:06:35Z (GMT) No. of bitstreams: 2 Dissertacão - Petain Jose Ferreira Neto - 2015.pdf: 1935098 bytes, checksum: 58b97940c1a11dda51ddc983a41e4bd9 (MD5) license_rdf: 19874 bytes, checksum: 38cb62ef53e6f513db2fb7e337df6485 (MD5)Made available in DSpace on 2016-06-28T13:06:36Z (GMT). No. of bitstreams: 2 Dissertacão - Petain Jose Ferreira Neto - 2015.pdf: 1935098 bytes, checksum: 58b97940c1a11dda51ddc983a41e4bd9 (MD5) license_rdf: 19874 bytes, checksum: 38cb62ef53e6f513db2fb7e337df6485 (MD5) Previous issue date: 2015-07-13The Cerrado is the second largest biome in Brazil. Such an environment has a different soil several other regions. There are various living beings in this biome, among them, the actinobacteria. They are microorganisms whose cell wall Gram-positive, >50% G+C (guanine + cytosine) in its DNA, produce substances with high economic value. Actinobacteria are major producers of enzymes useful to humans. L-asparaginase enzyme is used in acute limphoblastic leukemias therapy (ALL) also being used in the pre-treatment of foods rich in reducing sugars, inhibiting the formation of acrylamide. The aim of this study was to produce L-asparaginase enzyme isolated from a soil actinobacteria, characterize the enzyme and identify the species of bacteria isolated. A soil sample from Cerrado was collected and submited to a pretreatment. Chemotaxis of Actinoplanetes spores used for the selection of this organism. Nineteen morphspecies were recovered. We used a medium inducing L-asparaginase, and the pink halo around the colony measured. Isolated BC-A.1 produced the largest halo in less time, with an average enzyme content of 4.1. After the completion of the production steps, partial purification and optimization of enzymatic activity, it was realized that the enzyme produced by isolated morphospecies is very efficient at 40 ° C and pH 8.0. Biochemical, physiological and molecular tests were performed, and the observation with optical microscopy and scanning electron microscopy, but it was not possible to identify the species isolated, probably belongs to the genus Streptomyces.O Cerrado é o segundo maior bioma do Brasil. Tal ambiente possui um solo diferenciado de várias outras regiões. Existem vários seres vivos neste Bioma, dentre estes, as actinobactérias. São micro-organismos cujas células têm parede celular Gram-positiva, >50% de G+C (guanina + citosina) em seu DNA, produzem diversas substâncias com alto valor agregado. Actinobactérias são importantes produtores de enzimas úteis aos seres humanos. A enzima L-asparaginase é utilizada na terapia de Leucemias Linfoblásticas Agudas (ALL) sendo também empregada no pré-tratamento de alimentos ricos em açúcares redutores, inibindo a formação de acrilamida. O objetivo deste estudo foi produzir a enzima L-asparaginase a partir de uma actinobactéria isolada do solo, caracterizar a enzima e identificar a espécie do isolado bacteriano. Uma amostra do solo foi coletada no Cerrado e passou por um pré-tratamento. Foi utilizada a quimiotaxia dos esporos de Actinoplanetes para a seleção deste organismo. Foram recuperadas 19 morfoespécies. Utilizou-se um meio indutor de L-asparaginase, e o halo róseo ao redor da colônia medido. O isolado BC-A.1 produziu o maior halo em tempo menor, com índice enzimático médio de 4,1. Após a realização das etapas de produção, purificação parcial e otimização da atividade enzimática, percebeu-se que a enzima produzida pela morfoespécie isolada é muito eficiente a 40°C e pH 8,0. Foram realizados testes bioquímicos, fisiológicos e moleculares, bem como a observação em microscopia óptica e microscopia eletrônica de varredura, mas não foi possível identificar a espécie do isolado, provavelmente pertence ao gênero Streptomyces

Metodos de avaliação economica de projetos de explotação mineral

Orientador: Saul Barisnik SuslickDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de GeocienciasResumo: O objetivo do trabalho é o estudo dos métodos quantitativos usados no processo decisório da avaliação econômica de projetos de investimentos, do ponto de vista empresarial, visando o aproveitamento de jazidas minerais. O texto aprofunda as discussões sobre os pontos chaves do tema proposto e procura, de forma simples e acessível, esclarecer os aspectos cruciais de cada questão levantada. No capítulo inicial são apresentados os conceitos básicos de fluxo de caixa e de cálculo financeiro e os impactos resultantes da tributação nos projetos de mineração. O tema central está contido nos dois capítulos subseqüentes, onde cada método de avaliação é descrito, aplicado e comentado o seu emprego nas tomadas de decisão relativas às seguintes situações: aceitar/rejeitar um projeto isolado; selecionar "o melhor" (o mais atrativo) de um grupo de projetos mutuamente excludentes com horizontes iguais ou diferentes; e, escolher uma combinação ótima de alternativas independentes considerando haver restrição orçamentária. O capítulo a seguir apresenta a análise de sensibilidade como instrumento de identificação das variáveis estratégicas de um projeto. Finalmente, o último capítulo descreve a análise de risco mediante o emprego da técnica analítica e da simulação de Monte Carlo, como forma de enriquecimento dos resultados da avaliação econômicaAbstract: The purpose of this dissertation involves the study of quantitative methods applied to decision-making process for economic evaluation of investments aiming the availability of mineral resources. The text covers the key aspects of the mentioned subject and explains in simple form ali the necessary topics of mine evaluation. The first chapter presents the basic concepts of cash-flow, time value for the evaluation investment alternatives and the effect of taxation on mining properties and operations. The main part describes each evaluation method and their application, which was discussed under the decision-making environment of the following aspects: accept/reject an isolated project, select 'the best' project from a cluster of muttually exclusive projects with different or equal productive life and choose an optimal combination of independent alternatives based upon budget restrictions. The next chapter presents the sensitivity analysis as a tool for identification of strategic variables. Finally, the last chapter describes the risk analysis based upon of analytical technique and the Monte Carlo simulation in order to achieve better and richer results for economic evaluationMestradoAdministração e Politica de Recursos MineraisMestre em Geociência