Beyond technology: pottery reveals translocal social relations at a Bell Beaker Monumental Site in Central Europe

The Bell Beaker site near Brodek u Prostějova (Czechia) has yielded remains of a large timber construction accompanied by four symmetrical ritual deposits with numerous artefacts, including more than fifty ceramic vessels. Their decoration consists of incised patterns, in nineteen cases with preserved white inlaid incrustations. To investigate the social relations at this extraordinary site, a multi-analytical and micro-destructive approach was employed to determine the provenance and technology of the pottery and the composition of the white incrustations. The results indicate various origins for the pottery within the region and the presence of extra-regional fabrics and graphitic temper. The main raw materials for the white inlays were calcium carbonate (calcite), hydroxyapatite (bone), and bright clay. The mixing of decorative motifs and the variation in the shape and size of the beakers suggest unique manufacturing processes. These results lend support to the monumental site of Brodek serving as a ritual place for several communities from both local and wider areas.Introduction Materials and methods Results - Ceramic petrography and chemical composition White inlay incrustations - Micro Fourier transform infrared spectroscopy (μFTIR) - Micro X-ray diffraction (μXRD) - Scanning electron microscopy-electron dispersive spectroscopy (SEM-EDS) Metrics abd decoration analysis Discussion - Ceramic provenance and materials of white incrustations - Depositional semantics and ceramic patterns in the ritual features - Translocal social relationships and the circulation of ceramics and other artefacts Conclusio

Concurrent overexpression of amino acid permease AAP1(3a) and SUT1 sucrose transporter in pea resulted in increased seed number and changed cytokinin and protein levels

Using pea as our model crop, we sought to understand the regulatory control over the import of sugars and amino acids into the developing seeds and its importance for seed yield and quality. Transgenic peas simultaneously overexpressing a sucrose transporter and an amino acid transporter were developed. Pod walls, seed coats, and cotyledons were analysed separately, as well as leaves subtending developing pods. Sucrose, starch, protein, free amino acids, and endogenous cytokinins were measured during development. Temporal gene expression analyses (RT-qPCR) of amino acid (AAP), sucrose (SUT), and SWEET transporter family members, and those from cell wall invertase, cytokinin biosynthetic (IPT) and degradation (CKX) gene families indicated a strong effect of the transgenes on gene expression. In seed coats of the double transgenics, increased content and prolonged presence of cytokinin was particularly noticeable. The transgenes effectively promoted transition of young sink leaves into source leaves. We suggest the increased flux of sucrose and amino acids from source to sink, along with increased interaction between cytokinin and cell wall invertase in developing seed coats led to enhanced sink activity, resulting in higher cotyledon sucrose at process pea harvest, and increased seed number and protein content at maturity

Strategies in Taxonomy: Research in a Changing World: report of an electronic conference, May 2009

This EPBRS e-conference on “Strategies for Taxonomy: Research in a Changing World” focused on identifying the key research questions allowing taxonomy to address policy needs in a better way and, vice versa, allowing policy makers to get responses from taxonomists on specific subjects related to the use of taxonomic knowledge. Taxonomy, as a collectively assembled ‘Body of Knowledge’ formally started with the work of Linnaeus, is the most comprehensive and reliable source of information about biodiversity today. This includes specimen collections, character descriptions, geographic distributions, occurrence details, classification system(s), and links to associated information in literature and other resources. This accumulated ‘Body of Knowledge’ is well structured around the Linnaean system and is our baseline knowledge to monitor changes in biodiversity. Although there are few doubts that taxonomic information is essential for reliable environmental science, applied users often complain about the inability to get adequate access to taxonomic information to respond to -for instance- the biodiversity crisis. This ‘taxonomic impediment’ is recognised within taxonomy by currently attempting to expand its capacity to explore and accommodate more species and to improve the dissemination and use of taxonomic information on the internet. Due to these internal and external constraints, pushing taxonomy to advance its working routines and support the applied use of taxonomic information, taxonomy stands, at the moment, at a crossroads. Therefore it is critical for policy makers to make the right decisions for the future of taxonomy, now. This should, for instance, balance not only the emphasis stakeholders, researchers and society often place on new technologies and research tools but also the maintenance of core taxonomic research respecting the often exceptional level of expertise needed to explore and recognise species diversity. Finding the right connection between taxonomists and policy makers is complicated because of the different intermediate parties (e.g. data centres, agencies and stakeholders) frequently obscuring a profound understanding of the relevance of taxonomy for biodiversity assessments for policy makers, but also hampering a closer commitment of taxonomists to fundamental questions on what information is needed (at what level and at what depth) for policy makers to make the right decisions. I hope this e-conference has contributed to a better understanding among decision makers and taxonomists of the relevant scientific priorities for future strategic policy plans

sPlotOpen – An environmentally balanced, open‐access, global dataset of vegetation plots

Assessing biodiversity status and trends in plant communities is critical for understanding, quantifying and predicting the effects of global change on ecosystems. Vegetation plots record the occurrence or abundance of all plant species co-occurring within delimited local areas. This allows species absences to be inferred, information seldom provided by existing global plant datasets. Although many vegetation plots have been recorded, most are not available to the global research community. A recent initiative, called ‘sPlot’, compiled the first global vegetation plot database, and continues to grow and curate it. The sPlot database, however, is extremely unbalanced spatially and environmentally, and is not open-access. Here, we address both these issues by (a) resampling the vegetation plots using several environmental variables as sampling strata and (b) securing permission from data holders of 105 local-to-regional datasets to openly release data. We thus present sPlotOpen, the largest open-access dataset of vegetation plots ever released. sPlotOpen can be used to explore global diversity at the plant community level, as ground truth data in remote sensing applications, or as a baseline for biodiversity monitoring

Thermal differences between juveniles and adults increased over time in European forest trees

1. Woody species' requirements and environmental sensitivity change from seedlings to adults, a process referred to as ontogenetic shift. Such shifts can be increased by climate change. To assess the changes in the difference of temperature experienced by seedlings and adults in the context of climate change, it is essential to have reliable climatic data over long periods that capture the thermal conditions experienced by the individuals throughout their life cycle. 2. Here we used a unique cross-European database of 2,195 pairs of resurveyed forest plots with a mean intercensus time interval of 37 years. We inferred macroclimatic temperature (free-air conditions above tree canopies—representative of the conditions experienced by adult trees) and microclimatic temperature (representative of the juvenile stage at the forest floor, inferred from the relationship between canopy cover, distance to the coast and below-canopy temperature) at both surveys. We then address the long-term, large-scale and multitaxa dynamics of the difference between the temperatures experienced by adults and juveniles of 25 temperate tree species. 3. We found significant, but species-specific, variations in the perceived temperature (calculated from presence/absence data) between life stages during both surveys. Additionally, the difference of the temperature experienced by the adult versus juveniles significantly increased between surveys for 8 of 25 species. We found evidence of a relationship between the difference of temperature experienced by juveniles and adults over time and one key functional trait (i.e. leaf area). Together, these results suggest that the temperatures experienced by adults versus juveniles became more decoupled over time for a subset of species, probably due to the combination of climate change and a recorded increase of canopy cover between the surveys resulting in higher rates of macroclimate than microclimate warming. 4. Synthesis. We document warming and canopy-cover induced changes in the difference of the temperature experienced by juveniles and adults. These findings have implications for forest management adaptation to climate change such as the promotion of tree regeneration by creating suitable species-specific microclimatic conditions. Such adaptive management will help to mitigate the macroclimate change in the understorey layer

Combining biodiversity resurveys across regions to advance global change research

More and more ecologists have started to resurvey communities sampled in earlier decades to determine long-term shifts in community composition and infer the likely drivers of the ecological changes observed. However, to assess the relative importance of, and interactions among, multiple drivers joint analyses of resurvey data from many regions spanning large environmental gradients are needed. In this paper we illustrate how combining resurvey data from multiple regions can increase the likelihood of driver-orthogonality within the design and show that repeatedly surveying across multiple regions provides higher representativeness and comprehensiveness, allowing us to answer more completely a broader range of questions. We provide general guidelines to aid implementation of multi-region resurvey databases. In so doing, we aim to encourage resurvey database development across other community types and biomes to advance global environmental change research