Anestesisjuksköterskans uppfattning av WHO´s checklista för säkerhet vid operationer

Värdshälsoorganisationen (WHO), utarbetade 2007 en kirurgisk checklista för att stärka och öka patientsäkerheten vid kirurgiska ingrepp. I Region Skåne infördes checklistan 2009 för användande i skånsk sjukvård. Syfte: Att undersöka anestesisjuksköterskors uppfattning om hur checklistan används samt att beskriva eventuella skillnader i användandet på fyra sjukhus i Skåne. Metod: En tvärsnittsstudie med enkäter som besvarades av 68 respondenter genomfördes på fyra sjukhus i Skåne mars 2013. Resultat: sammantaget på de fyra sjukhusen i Skåne upplevs att patientsäkerheten ökat efter checklistans införande. Teamkänslan upplevs ha ökat men med variation både inom sjukhuset och mellan sjukhusen. Det upplevs även att checklistan alltid används vid planerade ingrepp men med en lägre frekvens och användandegrad vid akuta ingrepp. Konklusion: Anestesisjuksköterskor på fyra sjukhus i Skåne upplever generellt att checklistan används tillfredställande och att den bidragit till ökad patientsäkerhet och teamkänsla. Det upplevs dock fortfarande vissa brister i teamkänsla och checklistans användandefrekvens och i dess användandegrad

Book Review

reviewing Eyal Benvenisti & Moshe Hirsch eds., The Impact of International Law on International Cooperation: Theoretical Perspectives (2004

Boundary estimates for solutions to linear degenerate parabolic equations

Let Ω ⊂ R n be a bounded NTA-domain and let Ω T = Ω × (0, T ) for some T > 0. We study the boundary behaviour of non-negative solutions to the equation We assume that A(x, t) = {a ij (x, t)} is measurable, real, symmetric and that for some constant β ≥ 1 and for some non-negative and real-valued function λ = λ(x) belonging to the Muckenhoupt class A 1+2/n (R n ). Our main results include the doubling property of the associated parabolic measure and the Hölder continuity up to the boundary of quotients of non-negative solutions which vanish continuously on a portion of the boundary. Our results generalize previous results of Fabes, Kenig, Jerison, Serapioni, se

Correlational analysis for identifying genes whose regulation contributes to chronic neuropathic pain

<p>Abstract</p> <p>Background</p> <p>Nerve injury-triggered hyperexcitability in primary sensory neurons is considered a major source of chronic neuropathic pain. The hyperexcitability, in turn, is thought to be related to transcriptional switching in afferent cell somata. Analysis using expression microarrays has revealed that many genes are regulated in the dorsal root ganglion (DRG) following axotomy. But which contribute to pain phenotype versus other nerve injury-evoked processes such as nerve regeneration? Using the L5 spinal nerve ligation model of neuropathy we examined <b><it>differential </it></b>changes in gene expression in the L5 (and L4) DRGs in five mouse strains with contrasting susceptibility to neuropathic pain. We sought genes for which the degree of regulation correlates with strain-specific pain phenotype.</p> <p>Results</p> <p>In an initial experiment six candidate genes previously identified as important in pain physiology were selected for in situ hybridization to DRG sections. Among these, regulation of the Na⁺channel α subunit <it>Scn11a </it>correlated with levels of spontaneous pain behavior, and regulation of the cool receptor <it>Trpm8 </it>correlated with heat hypersensibility. In a larger scale experiment, mRNA extracted from individual mouse DRGs was processed on Affymetrix whole-genome expression microarrays. Overall, 2552 ± 477 transcripts were significantly regulated in the axotomized L5DRG 3 days postoperatively. However, in only a small fraction of these was the degree of regulation correlated with pain behavior across strains. Very few genes in the "uninjured" L4DRG showed altered expression (24 ± 28).</p> <p>Conclusion</p> <p>Correlational analysis based on in situ hybridization provided evidence that differential regulation of <it>Scn11a </it>and <it>Trpm8 </it>contributes to across-strain variability in pain phenotype. This does not, of course, constitute evidence that the others are unrelated to pain. Correlational analysis based on microarray data yielded a larger "look-up table" of genes whose regulation likely contributes to pain variability. While this list is enriched in genes of potential importance for pain physiology, and is relatively free of the bias inherent in the candidate gene approach, additional steps are required to clarify which transcripts on the list are in fact of functional importance.</p

A lake as a microcosm: reflections on developments in aquatic ecology

In the present study, we aim at relating Forbes' remarkable paper on "The lake as a microcosm", published 125 years ago, to the present status of knowledge in our own research group. Hence, we relate the observations Forbes made to our own microcosm, Lake Krankesjon in southern Sweden, that has been intensively studied by several research groups for more than three decades. Specifically, we focus on the question: Have we made any significant progress or did Forbes and colleagues blaze the trail through the unknown wilderness and we are mainly paving that intellectual road? We conclude that lakes are more isolated than many other biomes, but have, indeed, many extensions, for example, input from the catchment, fishing and fish migration. We also conclude that irrespective of whether lakes should be viewed as microcosms or not, the paper by Forbes has been exceptionally influential and still is, especially since it touches upon almost all aspects of the lake ecosystem, from individual behaviour to food web interactions and environmental issues. Therefore, there is no doubt that even if 125 years have passed, Forbes' paper still is a source of inspiration and deserves to be read. Hence, although aquatic ecology has made considerable progress over the latest century, Forbes might be viewed as one of the major pioneers and visionary scientists of limnology

Identification of Novel α-Synuclein Isoforms in Human Brain Tissue by using an Online NanoLC-ESI-FTICR-MS Method

Parkinson’s disease (PD) and Dementia with Lewy bodies (DLB) are neurodegenerative diseases that are characterized by intra-neuronal inclusions of Lewy bodies in distinct brain regions. These inclusions consist mainly of aggregated α-synuclein (α-syn) protein. The present study used immunoprecipitation combined with nanoflow liquid chromatography (LC) coupled to high resolution electrospray ionization Fourier transform ion cyclotron resonance tandem mass spectrometry (ESI-FTICR-MS/MS) to determine known and novel isoforms of α-syn in brain tissue homogenates. N-terminally acetylated full-length α-syn (Ac-α-syn1–140) and two N-terminally acetylated C-terminally truncated forms of α-syn (Ac-α-syn1–139 and Ac-α-syn1–103) were found. The different forms of α-syn were further studied by Western blotting in brain tissue homogenates from the temporal cortex Brodmann area 36 (BA36) and the dorsolateral prefrontal cortex BA9 derived from controls, patients with DLB and PD with dementia (PDD). Quantification of α-syn in each brain tissue fraction was performed using a novel enzyme-linked immunosorbent assay (ELISA)

Den manliga sjuksköterskan, En feminin sexuellt avvikande person med tvivelaktiga avsikter som vårdare

A literature review about attitudes and expectations on the male nurse.En litteraturstudie om attityder och förväntningar på den manliga sjuksköterskan

Den manliga sjuksköterskan, En feminin sexuellt avvikande person med tvivelaktiga avsikter som vårdare

A literature review about attitudes and expectations on the male nurse.En litteraturstudie om attityder och förväntningar på den manliga sjuksköterskan