Susceptibilidade de crianças asmáticas a infecções respiratórias

OBJECTIVE: A case-control study of patients with pneumonia was conducted to investigate whether wheezing diseases could be a risk factor. METHODS: A random sample was taken from a general university hospital in S. Paulo City between March and August 1994 comprising 51 cases of pneumonia paired by age and sex to 51 non-respiratory controls and 51 healthy controls. Data collection was carried out by two senior paediatricians. Diagnoses of pneumonia and presence of wheezing disease were independently established by each paediatrician for both cases and controls. Pneumonia was radiologically confirmed and repeatability of information on wheezing diseases was measured. Logistic regression analysis was used to identify risk factors. RESULTS: Wheezing diseases, interpreted as proxies of asthma, were found to be an important risk factor for pneumonia with an odds ratio of 7.07 (95%CI= 2.34-21.36), when the effects of bedroom crowding (odds ratio = 1.49 per person, 95%CI= 0.95-2.32) and of low family income (odds ratio = 5.59 against high family income, 95%CI= 1.38-22.63) were controlled. The risk of pneumonia attributable to wheezing diseases is tentatively calculated at 51.42%. CONCLUSION: It is concluded that at practice level asthmatics should deserve proper surveillance for infection and that at public health level pneumonia incidence could be reduced if current World Health Organisation's guidelines were reviewed as to include comprehensive care for this illness.OBJETIVO: Investigar, através de um estudo caso-controle de pacientes com pneumonia, se as doenças chiadoras poderiam constituir-se em fator de risco. MÉTODOS: De um hospital universitário, na cidade de São Paulo, Brasil, entre março e agosto de 1994, foi tomada uma amostra de 51 casos de pneumonia pareados por sexo e idade a 51 controles sadios e 51 controles não respiratórios. O diagnóstico de pneumonia e a presença de doença chiadora foram investigados de forma independente por cada pediatra tanto para casos quanto para controles. Foi confirmada pneumonia radiologicamente e a repetibilidade da informação sobre doença chiadora foi medida. Foi utilizada regressão logística para identifição de riscos. RESULTADOS: As doenças chiadoras, entendidas como representantes de asma, mostraram ser importante fator de risco para pneumonia, com um odds ratio de 7,07 (IC95%= 2,34-21,36), controlados os efeitos de aglomeração no quarto de dormir (odds ratio de 1,49 por pessoa a mais no quarto, IC95%= 0,95-2,32) e a baixa renda familiar (odds ratio de 5,59 contra alta renda familiar, IC95%= 1,38-22,63). O risco atribuível às doenças chiadoras foi calculado de forma exploratória em 51,42%. CONCLUSÃO: Conclui-se que os clínicos devem ter atenção sobre asmáticos para o risco de infecção e que ao nível da saúde pública a incidência de pneumonia poderia ser reduzida se as orientações atuais da Organização Mundial da Saúde pudessem ser revistas para oferecer atenção integral para os doentes

Black-bounce solution in kk-essence theories

In the present work, we construct black-bounce configurations in the context of $k$-essence theory. The solutions have a regular metric function at the origin. The area metric function is linked to the black-bounce area initially considered by Simpson-Visser, $\Sigma^2=x^2+a^2$. Subsequently, the expressions for the scalar field and scalar potential corresponding to the found solutions are determined, exhibiting phantom behavior everywhere due to violation of Null Energy Condition $(NEC^\phi)$. The Kretschmann scalar is regular throughout spacetime, and the geodesics are complete. The energy conditions are analyzed, verifying that the null $(NEC^\phi_1)$ and dominant energy conditions $(DEC^\phi_1)$ are violated inside and outside the event horizon. Finally, the extrinsic curvature method was applied to determine the sign of the mass on the junction surface.Comment: 13 pages, 15 figure

Characterisation by molecular biology and MALDI-TOF ICMS of zygomycete Gongronella sp. isolated from an Alentejo vineyard soil

Zygomycete Gongronella sp. CCMI 1101 (MUM 10.263) was isolated from an Alentejo vineyard soil submitted to repeated treatments with the fungicide metalaxyl. This fungicide is widely used against Oomycetes species that causing downy mildew. After selective enrichment, adapted Gongronella sp. CCMI 1100 (MUM 10.262) showed high tolerance to metalaxyl with EC50 of 36.1 mg L-1. Additionally, this adapted fungus was able to use metalaxyl as main carbon and energy source. Afterwards, both Gongronella strains (non-adapted and metalaxyl adapted strains) were submitted to a polyphasic approach identification and characterisation. In contrast, proteomic analysis showed spectra with substantial quantitatively phenotypic differences between both strains that could be a consequence of a strain adaptation to metalaxyl. In conclusion, Gongronella sp. CCMI 1101 is now under further taxonomic studies and the current results suggest that the adapted strain CCMI 1100 can be explored in soil bioremediation for metalaxyl-degradation

Contamination of resin-matrix composites on chairside handling using latex or nitrile gloves: an in vitro study

The aim of this study was to evaluate the efect of diferent gloves and clinical environment on the contamination of resinmatrix composites for restorative dentistry. Specimens of nano-hybrid resin-matrix composite (n=6) were divided in groups regarding the handling with (A) clinical spatula; (B) latex gloves; (C) latex or (D) nitrile gloves with human saliva; (E) latex or (F) nitrile gloves with human blood. After light curing, groups of specimens were analyzed by optical microscopy at magnifcation ranging from x30 up to x500 and by scanning electron microscopy at diferent magnifcation ranging from x50 up to x8000. Handling of resin-matrix composites with unpowdered nitrile gloves or clinical spatulas avoided the presence of contaminants. However, agglomerates of the resin-matrix composite itself became entrapped leading to a heterogenous morphological aspect. SEM images revealed the presence of corn-derived starch released from the powdered gloves. Also, the formation of micro-spaces (voids) occurred after handling with powdered latex gloves. Specimens handled with both type of gloves contaminated with saliva showed a conditioning layer composed of glycoproteins rolls and compounds including calcium-based chlorides, phosphates, and carbonates. Also, blood products were transferred from the contaminated gloves to the resin-matrix composites after handling. Thus, resin-matrix composite restorations are susceptible to contamination with debris from powdered gloves. Also, saliva or blood debris become adsorbed and entrapped in the resin-matrix composites during clinical handling leading to the presence of defects such macro- and micro-scale voids or contaminant agglomerates.info:eu-repo/semantics/publishedVersio

Effect of inorganic fillers on the light transmission through traditional or flowable resin-matrix composites for restorative dentistry

Objectives: The aim of this in vitro study was to evaluate the light transmission through five different resin-matrix composites regarding the inorganic filler content. Methods: Resin-matrix composite disc-shaped specimens were prepared on glass molds. Three traditional resin-matrix composites contained inorganic fillers at 74, 80, and 89 wt. % while two flowable composites revealed 60 and 62.5 wt. % inorganic fillers. Light transmission through the resin-matrix composites was assessed using a spectrophotometer with an integrated monochromator before and after light curing for 10, 20, or 40s. Elastic modulus and nanohardness were evaluated through nanoindentation’s tests, while Vicker’s hardness was measured by micro-hardness assessment. Chemical analyses were performed by FTIR and EDS, while microstructural analysis was conducted by optical microscopy and scanning electron microscopy. Data were evaluated using two-way ANOVA and Tukey’s test (p < 0.05). Results: After polymerization, optical transmittance increased for all specimens above 650-nm wavelength irradiation since higher light exposure time leads to increased light transmittance. At 20- or 40-s irradiation, similar light transmittance was recorded for resin composites with 60, 62, 74, or 78–80 wt. % inorganic fillers. The lowest light transmittance was recorded for a resin-matrix composite reinforced with 89 wt. % inorganic fillers. Thus, the size of inorganic fillers ranged from nano- up to micro-scale dimensions and the high content of micro-scale inorganic particles can change the light pathway and decrease the light transmittance through the materials. At 850-nm wavelength, the average ratio between polymerized and non-polymerized specimens increased by 1.6 times for the resin composite with 89 wt. % fillers, while the composites with 60 wt. % fillers revealed an increased ratio by 3.5 times higher than that recorded at 600-nm wavelength. High mean values of elastic modulus, nano-hardness, and micro-hardness were recorded for the resin-matrix composites with the highest inorganic content. Conclusions: A high content of inorganic fillers at 89 wt.% decreased the light transmission through resin-matrix composites. However, certain types of fillers do not interfere on the light transmission, maintaining an optimal polymerization and the physical properties of the resin-matrix composites. Clinical significance: The type and content of inorganic fillers in the chemical composition of resin-matrix composites do affect their polymerization mode. As a consequence, the clinical performance of resin-matrix composites can be compromised, leading to variable physical properties and degradation. Graphical Abstract: [Figure not available: see fulltext.].info:eu-repo/semantics/publishedVersio

Development of a water-in-oil-in-water multiple emulsion system integrating biomimetic aqueous-core lipid nanodroplets for protein entity stabilization. Part I: experimental factorial design

Lipid nanoballoons integrating multiple emulsions of the type water-in-oil-in-water enclose, at least in theory, a biomimetic aqueous-core suitable for housing hydrophilic biomolecules such as proteins, peptides and bacteriophage particles. The research effort entertained in this paper reports a full statistical 23x31 factorial design study (three variables at two levels and one variable at three levels) to optimize biomimetic aqueous-core lipid nanoballoons for housing hydrophilic protein entities. The concentrations of protein, lipophilic and hydrophilic emulsifiers, and homogenization speed were set as the four independent variables, whereas the mean particle hydrodynamic size (HS), zeta potential (ZP) and polydispersity index (PI) were set as the dependent variables. The V23x31 factorial design constructed led to optimization of the higher (+1) and lower (-1) levels, with triplicate testing for the central (0) level, thus producing thirty three experiments and leading to selection of the optimized processing parameters as 0.015% (w/w) protein entity, 0.75% (w/w) lipophilic emulsifier (soybean lecithin) and 0.50% (w/w) hydrophilic emulsifier (poloxamer 188). In the present research effort, statistical optimization and production of protein derivatives encompassing full stabilization of their three-dimensional structure, has been attempted via housing said molecular entities within biomimetic aqueous-core lipid nanoballoons integrating a multiple (W/O/W) emulsion

Characterization of a water-in-oil-in-water multiple emulsion integrating biomimetic aqueous-core lipid nanoballoons housing protein entities

Project funding by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, São Paulo, Brazil) ( FAP ESP Ref. No. 2013/03181 - 6, Project PneumoPhageKill

Correlação entre o conteúdo inorgânico e a polimerização da matriz orgânica das resinas compostas para restaurações dentárias: uma revisão narrativa

Introduction: In recent years, resin-matrix composite materials have revealed a fast technological improvement for dental applications. However, there are still some drawbacks related to the chemical composition, polymerization, and mechanical properties of resin-matrix composites with consequences in long-term clinical success. Objective: This study aimed to perform a narrative review regarding the effects of inorganic fillers on the organic matrix polymerization of resin-matrix composites. Materials and Methods: A search was performed in PubMed using relevant related key terms related to the chemical composition, properties and polymerization of resin matrix composites. Relevant studies published between 2001 and 2021 were selected. Results: The studies included in the present research provided relevant information on the chemical composition and properties of resin composites and polymerization factors, including the time, wavelength mode, and equipment. Studies have reported that silica and silicate nano-scale particles improve light transmission through resin-matrix composites and the degree of conversion of monomers in the organic matrix. Micrometric particles with a high refractive index can decrease the degree of conversion of monomers in the organic matrix. Conclusion: The polymerization of resin-matrix composites is affected by light scattering due to the type of inorganic particles and the differences in refractive indexes of inorganic and organic contents.Introdução: Nos últimos anos, as resinas compostas têm sido melhoradas em termos tecnológicos, permitindo o alcance de melhores prognósticos na medicina dentária. Entretanto, subsistem algumas limitações relacionadas com a composição química e as propriedades das resinas compostas com consequências no sucesso clínico a longo prazo. Objetivo: O objetivo deste trabalho foi realizar uma revisão narrativa sobre a influência da componente inorgânica na polimerização da matriz orgânica das resinas compostas usadas para restauração dentária. Materiais e Métodos: A pesquisa foi realizada na plataforma PubMed utilizando termos de pesquisa relacionados com a composição química, propriedades e a polimerização das resinas compostas, para seleção de artigos relevantes ao tema. Resultados: Os estudos identificados reportam diferentes fatores relacionados com a polimerização incluindo modo, tempo, equipamento e a composição química das resinas compostas. Os estudos indicam que partículas nanométricas à base de sílica ou silicatos promovem uma maior transmissão de luz à matriz orgânica o que favorece a polimerização do material. Por outro lado, partículas micrométricas com um alto índice de refração podem diminuir o grau de conversão dos monómeros da matriz orgânica. Conclusão: A polimerização das resinas compostas é afetada pela dispersão da luz, devido ao tamanho das partículas inorgânicas e aos índices de refração da componente orgânica e inorgânica. De fato, um maior controlo da composição química e do tamanho de partículas inorgânicas aumenta o grau de polimerização das resinas compostas