Hierarchical Quantification of Utilization Rate and Related Indicators of Mixed-Use High-Rise Buildings

Mixed-use high-rise buildings are vertical superpositions of various business types in the category of mixed-use development. It has become a highly intensive organizational form in the urban high-density environment. Under China’s “height limit” policy, the simple superposition of business types does not meet the government requirements for planning, construction, and management, and does not fully utilize the advantages of the mixed development mode. The single utilization rate index used in the past could not accurately describe such buildings’ usage value and spatial variation characteristics. In this study, a quantitative analysis of data from eight construction projects was carried out, and a utilization rate index system was established at three levels, namely, the typical floor utilization rate k1, business utilization rate k2, and building utilization rate k3. In terms of k1, the emphasis was placed on the design elements of the mixed-use development and the variation of relative indicators. In k2 and k3, it was found that the business type, floor area, and utilization rate were negatively correlated. In conclusion, by establishing a hierarchical utilization rate calculation method, the efficiency values and design characteristics of mixed-use high-rise (MUHR) buildings were explored, which provide references for the future design of such building

A double-layer Huygens’ metasurface with complete phase coverage and its dual-polarized meta-lens antenna application

A novel double-layer Huygens’ unit with dual-polarized response and complete phase coverage has been proposed for highly efficient meta-lens antenna application. The designed unit is a symmetric structure in which the double-layer metallic patterns not only act as the electric dipoles but also induce the magnetic dipoles. The balance between the initial electric dipole and the induced magnetic dipole makes the impedance of the metasurface match with that of free space, thus allowing efficient transmission of electromagnetic waves. Moreover, full transmission phase shift and near-unit transmission amplitude can be achieved simultaneously by change the structural parameters of the unit. Utilizing such electromagnetic properties, a dual-polarized meta-lens consisting of 35 × 35 units with size of 161 × 161 mm2 is designed, fabricated and measured. The results exhibit its good radiation performance. The maximum gain at a frequency of 28 GHz reaches 30.8 dBi, with an aperture efficiency of 42.3%. The 3-dB gain bandwidth reaches 12.5%, covering the frequency range of 26.7–30.2 GHz. The simple structure of the designed dual-polarized metasurface, high gain and high antenna efficiency make it an important antenna engineering role

Fractional creep model and experimental study of unsaturated silty clay in Fuyang

Due to the long-term overdraft of groundwater in Fuyang, silty clay in this area has been in an unsaturated state for a long time, which caused ground subsidence and threatened the safety of engineering construction. Creep is an important part of ground subsidence, but it is easily ignored in ground subsidence caused by groundwater overdraft. Therefore, in order to explore the creep behavior in this environment, a series of triaxial creep tests of unsaturated soil were conducted to research the effects of deviator stress, matrix suction and net confining pressure on creep. Then, based on analysis results of the geometric characteristics of the test creep curves, an improved fractional order Nishihara model of unsaturated soil under triaxial stress conditions was constructed by using fractional calculus theory. Finally, the effectiveness of improved fractional order Nishihara model was verified based on cooperation search algorithm and minimum mean square error principle. The simulation results show that the fitting curves of improved fractional order Nishihara model are in good agreement with the test curves, and it is feasible and effective to describe the creep characteristics of unsaturated silty clay in Fuyang

Quantitative analysis of multi-components by single marker method combined with UPLC-PAD fingerprint analysis based on saikosaponin for discrimination of Bupleuri Radix according to geographical origin

Background: Saikosaponins are regarded as one of the most likely antipyretic constituents of Bupleuri Radix, establishing a comprehensive method that can reflect both the proportion of all constituents and the content of each saikosaponin is critical for its quality evaluation.Methods: In this study, the combination method of quantitative analysis of multiple components with a single marker (QAMS) and fingerprint was firstly established for simultaneous determination of 7 kinds of saikosaponins in Bupleuri Radix by ultra-high performance liquid chromatography (UPLC).Results: The results showed that saikosaponin d was identified as the optimum IR by evaluating the fluctuations and stability of the relative calibration factors (RCFs) under four different conditions. The new QAMS method has been confirmed to accurately quantify the 7 kinds of saikosaponins by comparing the obtained results with those obtained from external standard method and successfully classify the 20 batches of Bupleuri Radix from 8 provinces of China. The experimental time of fingerprint was significantly reduced to approximate 0.5 h through UPLC-PAD method, a total of 17 common peaks were identified.Conclusion: The QAMS-fingerprint method is feasible and reliable for the quality evaluation of Bupleuri Radix. This method could be considered to be spread in the production enterprises of Bupleuri Radix

Diffusion-weighted imaging lesions after endovascular treatment of cerebral aneurysms: A network meta-analysis

BackgroundThromboembolism is one of the common complications in endovascular treatments including coiling alone, stent-assisted coiling (SAC), balloon-assisted coiling (BAC), and flow-diverting (FD) stents. Such treatments are widely used in intracranial aneurysms (IAs), which usually present as positive lesions in diffusion-weighted imaging (DWI). Whether these adjunctive techniques increase postprocedural DWI-positive lesions after endovascular treatment remains unclear.MethodsA thorough electronic search for the literature published in English between January 2000 and October 2022 was conducted on PubMed, Medline, and EMBASE. Eighteen studies (3 cohort studies and 15 case–control studies) involving 1,843 patients with unruptured IAs (UIAs) were included. We performed a frequentist framework network meta-analysis (NMA) to compare the rank risks of cerebral thromboembolism of the above four endovascular treatments. The incoherence test was used to analyze the statistical disagreement between direct and indirect evidence. Funnel plots were used to analyze publication bias.ResultsThe incidences of DWI lesions in patients who received FD stents, SAC, BAC, and coiling alone were 66.1% (109/165), 37.6% (299/795), 31.1% (236/759), and 25.6% (236/921). The incidence of DWI lesions in patients who received FD stents was higher than that in patients who received SAC [OR: 2.40; 95% CI (1.15, 5.00), P < 0.05], BAC [OR: 2.62; 95% CI (1.19, 5.77), P < 0.05], or coiling alone [OR: 2.77; 95% CI (1.26, 6.07), P < 0.05]. The incoherence test showed preferable consistency in this NMA. No obvious publication bias was found in the funnel plot.ConclusionFD stent placement brings more ischemic lesions identified by DWI than any other procedures for patients with UIA. The characteristics of FD stents may result in a high incidence of DWI lesions

Multiomics integration reveals the effect of Orexin A on glioblastoma

Objectives: This study involved a multi-omics analysis of glioblastoma (GBM) samples to elaborate the potential mechanism of drug treatment.Methods: The GBM cells treated with or without orexin A were acquired from sequencing analysis. Differentially expressed genes/proteins/metabolites (DEGs/ DEPs/ DEMs) were screened. Next, combination analyses were conducted to investigate the common pathways and correlations between the two groups. Lastly, transcriptome-proteome-metabolome association analysis was carried out to determine the common pathways, and the genes in these pathways were analyzed through Kaplan-Meier (K-M) survival analysis in public databases. Cell and animal experiments were performed to investigate the anti-glioma activity of orexin A.Results: A total of 1,527 DEGs, 52 DEPs, and 153 DEMs were found. Moreover, the combination analyses revealed that 6, 4, and 1 common pathways were present in the transcriptome-proteome, proteome-metabolome, and transcriptome-metabolome, respectively. Certain correlations were observed between the two data sets. Finally, 11 common pathways were discovered in association analysis, and 138 common genes were screened out in these common pathways. Six genes showed significant differences in terms of survival in both TCGA and CGGA. In addition, orexin A inhibited the proliferation, migration, and invasion of glioma in vitro and in vivo.Conclusion: Eleven common KEGG pathways with six common genes were found among different omics participations, revealing the underlying mechanisms in different omics and providing theoretical basis and reference for multi-omics research on drug treatment

Haploinsufficiency of CYP8B1 associates with increased insulin sensitivity in humans

10.1172/JCI152961The Journal of clinical investigation13221e152961

Characterization of the histone methyltransferase PRDM9 using biochemical, biophysical and chemical biology techniques

PRDM proteins have emerged as important regulators of disease and developmental processes. To gain insight into the mechanistic actions of the PRDM family, we have performed comprehensive characterization of a prototype member protein, the histone methyltransferase PRDM9, using biochemical, biophysical and chemical biology techniques. In the present paper we report the first known molecular characterization of a PRDM9-methylated recombinant histone octamer and the identification of new histone substrates for the enzyme. A single C321P mutant of the PR/SET domain was demonstrated to significantly weaken PRDM9 activity. Additionally, we have optimized a robust biochemical assay amenable to high-throughput screening to facilitate the generation of small-molecule chemical probes for this protein family. The present study has provided valuable insight into the enzymology of an intrinsically active PRDM protein

Analysis of the Causes of Cracks in the Bottom Floor of the Underground Garage of the Hefei Government Affairs Center by using 3D Finite-Element Analysis

A three-dimensional finite-element software program is used in this study to analyze the causes of cracks in an underground garage. Numerous cracks, serious and regular alike, can be found in the underground garage of the Hefei Government Affairs Center. These cracks are mainly located around the central part of the bottom floor within a 44.6– 57.8 m radius. To explore the causes of the cracks, two attempts are made. On one hand, on-site crack detection and underground water monitoring are conducted. On the other hand, the finite-element software program ANSYS is adopted to establish a finite-element model for the floor–foundation and connecting beam–foundation soil systems of the underground garage. Furthermore, the influences of the underground foundation, underground water level, soil expansion, and Poisson ratio on the bottom floor are calculated and analyzed. On the basis of the calculation and monitoring results, the following conclusion can be made: underground water is the main cause of the bottom floor cracks because underground water exerts a pushing force from the bottom and causes the expansibility of expansive soil. The study aims to provide a theoretical basis for the treatment of cracking in the Hefei Government Affairs Center, and offer a reference for the design, construction, and maintenance of similar projects

Agarwood Alcohol Extract Ameliorates Isoproterenol-Induced Myocardial Ischemia by Inhibiting Oxidation and Apoptosis

Agarwood is a traditional medicine used for treating some diseases, including painful and ischemic diseases. This study was carried out to investigate the potential cardioprotective effect of the whole-tree agarwood-inducing technique-produced agarwood alcohol extract (WTAAE) on isoproterenol- (ISO-) induced myocardial ischemia (MI) in rats and explore the underlying molecular mechanisms. Compared to the MI group, WTAAE pretreatment significantly improved ST wave abnormal-elevation, mitigated myocardial histological damage; decreased creatinine kinase (CK), lactate dehydrogenase (LDH), alanine transaminase (ALT), and aspartate transaminase (AST) levels; reduced hydrogen peroxide (H2O2) and lipid peroxide (LPO) production; and increased total antioxidant capacity (T-AOC) and catalase (CAT) activities. Moreover, agarwood alcohol extracts (AAEs) markedly enhanced the mRNA levels of Nrf2-ARE pathway, and Bcl-2 reduced the apoptotic Bax family mRNA expressions. In addition, the effect of WTAAE was greater than that of wild agarwood alcohol extract (WAAE) and burning-chisel-drilling agarwood alcohol extract (FBAAE). All of these data indicate that WTAAE exerted the protective effects of MI, and its mechanism was associated with upregulating Nrf2-ARE and suppressing Bcl-2 pathways