Assessment of sampling methods to estimate horseshoe crab (Limulus polyphemus L.) egg density in Delaware Bay

Each spring horseshoe crabs (Limulus polyphemus L.) emerge from Delaware Bay to spawn and deposit their eggs on the foreshore of sandy beaches (Shuster and Botton, 1985; Smith et al., 2002a). From mid-May to early June, migratory shorebirds stopover in Delaware Bay and forage heavily on horseshoe crab eggs that have been transported up onto the beach (Botton et al., 1994; Burger et al., 1997; Tsipoura and Burger, 1999). Thus, estimating the quantity of horseshoe crab eggs in Delaware Bay beaches can be useful for monitoring spawning activity and assessing the amount of forage available to migratory shorebirds

Impacts of plant invasions can be reversed through restoration: A regional meta-analysis of faunal communities

We quantified the effects of invasive Phragmites australis on estuarine faunal communities using meta-analysis to compare invaded to uninvaded marshes and then evaluated whether ecological restoration could reverse those effects. Relative to uninvaded marshes, the quantity and condition of fauna in invaded marshes was significantly poorer. We detected negative impacts to fauna residing in the mid-Atlantic but not in New England and to fauna utilizing the marsh surface but not to those inhabiting tidal creeks. By taxonomic group, we found that the invasion negatively affected nekton but not invertebrates. Both adult and sub-adult nekton were adversely affected, although the magnitude of the effect on the sub-adults was four times greater than that for adults. Our results indicate that negative effects on fauna within the mid-Atlantic region largely drove the overall results. When restored marshes were compared to uninvaded marshes there were no significant differences across all metrics assessed, suggesting that the negative impacts of the invasion were reversed. A separate qualitative review of trophic data indicated that benthic microalgae and dominant vascular plants are important primary producers at the base of the food web in uninvaded, invaded, and restored salt marshes but the overall quantity and importance of microalgae to diet decreased in highly invaded systems due to decreased light, potentially reducing energy availability. Our analyses revealed that while estuarine communities are adversely affected by P. australis, impacts vary by region, habitat, taxonomic group, and life history stage and that restoration can reverse long-term effects over relatively short time scales. © 2013 Springer Science+Business Media Dordrecht

Assessing long-term population trends of wood frogs using egg-mass counts

In North America, most efforts to monitor pond-breeding anurans have focused on call surveys. Egg-mass counts offer an alternative monitoring strategy that has been used extensively in Europe because this technique can produce precise and accurate estimates of annual reproductive effort at many study sites. We surveyed egg masses of Wood Frogs (Lithobates sylvaticus) at 18 ponds for up to 16 years from 1993-2008 in the largest contiguous forest tract in southern New England. We detected an average of 441.5 ± 343.7 egg masses per pond. Based on annual egg-mass counts, coefficients of variation (CV) were slightly higher than previous estimates for this species. We detected no relationship between mean annual population size and CV or between length of time series and CV. Population fluctuations in these ponds exhibited evidence of annual synchrony, in part because annual fluctuations at individual ponds were large enough that it was difficult to assess differences in population trends among ponds. However, the overall trend suggests this population was probably increasing slightly, which was expected because ponds were located in contiguous forest that remained intact during the study. Egg-mass counts appear to represent a feasible technique to monitor Wood Frog populations, given that all local breeding ponds are monitored. © 2011 Society for the Study of Amphibians and Reptiles

Mendelian randomization analyses suggest a role for cholesterol in the development of endometrial cancer.

Blood lipids have been associated with the development of a range of cancers, including breast, lung and colorectal cancer. For endometrial cancer, observational studies have reported inconsistent associations between blood lipids and cancer risk. To reduce biases from unmeasured confounding, we performed a bidirectional, two-sample Mendelian randomization analysis to investigate the relationship between levels of three blood lipids (low-density lipoprotein [LDL] and high-density lipoprotein [HDL] cholesterol, and triglycerides) and endometrial cancer risk. Genetic variants associated with each of these blood lipid levels (P < 5 × 10-8 ) were identified as instrumental variables, and assessed using genome-wide association study data from the Endometrial Cancer Association Consortium (12 906 cases and 108 979 controls) and the Global Lipids Genetic Consortium (n = 188 578). Mendelian randomization analyses found genetically raised LDL cholesterol levels to be associated with lower risks of endometrial cancer of all histologies combined, and of endometrioid and non-endometrioid subtypes. Conversely, higher genetically predicted HDL cholesterol levels were associated with increased risk of non-endometrioid endometrial cancer. After accounting for the potential confounding role of obesity (as measured by genetic variants associated with body mass index), the association between genetically predicted increased LDL cholesterol levels and lower endometrial cancer risk remained significant, especially for non-endometrioid endometrial cancer. There was no evidence to support a role for triglycerides in endometrial cancer development. Our study supports a role for LDL and HDL cholesterol in the development of non-endometrioid endometrial cancer. Further studies are required to understand the mechanisms underlying these findings

A common variant at the TERT-CLPTM1L locus is associated with estrogen receptor-negative breast cancer

Estrogen receptor (ER)-negative breast cancer shows a higher incidence in women of African ancestry compared to women of European ancestry. In search of common risk alleles for ER-negative breast cancer, we combined genome-wide association study (GWAS) data from women of African ancestry (1,004 ER-negative cases and 2,745 controls) and European ancestry (1,718 ER-negative cases and 3,670 controls), with replication testing conducted in an additional 2,292 ER-negative cases and 16,901 controls of European ancestry. We identified a common risk variant for ER-negative breast cancer at the TERT-CLPTM1L locus on chromosome 5p15 (rs10069690: per-allele odds ratio (OR) = 1.18 per allele, P = 1.0 x 10(-10)). The variant was also significantly associated with triple-negative (ER-negative, progesterone receptor (PR)-negative and human epidermal growth factor-2 (HER2)-negative) breast cancer (OR = 1.25, P = 1.1 x 10(-9)), particularly in younger women (&lt;50 years of age) (OR = 1.48, P = 1.9 x 10(-9)). Our results identify a genetic locus associated with estrogen receptor negative breast cancer subtypes in multiple populations

Nya extra-posten. (N:r 50)

Estrogen receptor (ER)-negative breast cancer shows a higher incidence in women of African ancestry compared to women of European ancestry. In search of common risk alleles for ER-negative breast cancer, we combined genome-wide association study (GWAS) data from women of African ancestry (1,004 ER-negative cases and 2,745 controls) and European ancestry (1,718 ER-negative cases and 3,670 controls), with replication testing conducted in an additional 2,292 ER-negative cases and 16,901 controls of European ancestry. We identified a common risk variant for ER-negative breast cancer at the TERT-CLPTM1L locus on chromosome 5p15 (rs10069690: per-allele odds ratio (OR) = 1.18 per allele, P = 1.0 × 10?10). The variant was also significantly associated with triple-negative (ER-negative, progesterone receptor (PR)-negative and human epidermal growth factor-2 (HER2)-negative) breast cancer (OR = 1.25, P = 1.1 × 10?9), particularly in younger women (&lt;50 years of age) (OR = 1.48, P = 1.9 × 10?9). Our results identify a genetic locus associated with estrogen receptor negative breast cancer subtypes in multiple populations