Beta-Lactamase Repressor BlaI Modulates Staphylococcus aureus Cathelicidin Antimicrobial Peptide Resistance and Virulence.

BlaI is a repressor of BlaZ, the beta-lactamase responsible for penicillin resistance in Staphylococcus aureus. Through screening a transposon library in S. aureus Newman for susceptibility to cathelicidin antimicrobial peptide, we discovered BlaI as a novel cathelicidin resistance factor. Additionally, through integrational mutagenesis in S. aureus Newman and MRSA Sanger 252 strains, we confirmed the role of BlaI in resistance to human and murine cathelidicin and showed that it contributes to virulence in human whole blood and murine infection models. We further demonstrated that BlaI could be a target for innate immune-based antimicrobial therapies; by removing BlaI through subinhibitory concentrations of 6-aminopenicillanic acid, we were able to sensitize S. aureus to LL-37 killing

The Future is Hera! Analyzing Astronomical Over the Internet

Hera is the data processing facility provided by the High Energy Astrophysics Science Archive Research Center (HEASARC) at the NASA Goddard Space Flight Center for analyzing astronomical data. Hera provides all the pre-installed software packages, local disk space, and computing resources need to do general processing of FITS format data files residing on the users local computer, and to do research using the publicly available data from the High ENergy Astrophysics Division. Qualified students, educators and researchers may freely use the Hera services over the internet of research and educational purposes

Correlation of Adherence to the 2012 Infectious Diseases Society of America Practice Guidelines with Patient Outcomes in the Treatment of Diabetic Foot Infections in an Outpatient Parenteral Antimicrobial Programme

Aim To evaluate adherence to the 2012 Infectious Diseases Society of America practice guidelines for the management of patients with diabetic foot infections and to determine an association between adherence and clinical outcome. Methods A retrospective chart review was performed to evaluate the management and clinical outcomes of patients with diabetic foot infections treated with outpatient parenteral antimicrobial therapy between 1 January 2011 and 30 June 2012 at Wishard Health Services/Eskenazi Health. Adherence to individual Infectious Diseases Society of America diabetic foot infection treatment guideline recommendations was measured, and then assessed in relation to clinical outcome. Results A total of 57 patients (61% male, mean age 54 years) with moderate to severe diabetic foot infection met the inclusion criteria. None of the treatment courses of these patients adhered to all the Infectious Diseases Society of America guideline recommendations. The recommendations most frequently adhered to were consultation of appropriate multidisciplinary teams (n=54, 94.7%) and performance of diagnostic imaging (n=52, 89.5%). The recommendations least frequently adhered to were diabetic foot wound classification scoring on admission (n=0, 0%), appropriate culture acquisition (n=12, 21.2%), surgical intervention when indicated (n=32, 46.2%) and appropriate empiric antibiotic selection (n=34, 59.7%). Of 56 patients, 52 (92.9%) experienced clinical cure at the end of outpatient parenteral antimicrobial therapy compared with 34 of 53 patients (64%) at 6 months after the completion of therapy. Adherence to individual guidelines was not associated with clinical outcome. Patients who experienced treatment failure were more likely to have severe diabetic foot infection or peripheral neuropathy. Conclusions Adherence to the Infectious Diseases Society of America diabetic foot infection guideline recommendations was found to be suboptimal in the present study. The effect of adhering to individual Infectious Diseases Society of America diabetic foot infection recommendations on clinical outcome needs to be investigated

Markov-parameter-based adaptive control of 3-axis angular velocity in a six-degree-of-freedom Stewart platform

Abstract-Stewart platforms are complex mechanical devices used throughout industry for vibration testing and precision pointing applications. These platforms are nonlinear, strongly coupled MIMO systems. For a six-degree-of-freedom Stewart platform, we consider the problem of three-degree-of-freedom angular-velocity command following. Static nonlinearity inherent in the platform is analyzed, and a closed-loop setup for adaptive command-following control is described. A review of the Markov-parameter-based adaptive control algorithm is given, along with the OKID system identification algorithm, test procedures, and experimental results

The importance of considering the treatment and care of family pets in domestic violence risk assessments

A significant part of the health visiting role is to work with families where there is domestic violence. Pets are often regarded as members of the family and as such are additional victims in environments where domestic violence is perpetrated. This paper explores the co-existence of domestic violence and animal cruelty and the implications of the use of animal cruelty to exercise coercive control over intimate partners in terms of the dangerousness of the abuser. It also considers the impact of animal cruelty on the health and social and emotional development of children. In domestic violence situations, child safety should be held paramount and adult safety a priority; the authors argue that in the pursuit of the best outcomes for children and adults, health visitors should be cognisant of the treatment and care of family pets in their assessment and in planning interventions to support families in changing their situation. An overview is provided of the domestic violence risk assessment tools that refer to animal cruelty as a contributory factor for serious harm and the animal welfare services available to help families escaping domestic violence

Chandra Observation of Luminous and Ultraluminous X-ray Binaries in M101

X-ray binaries in the Milky Way are among the brightest objects on the X-ray sky. With the increasing sensitivity of recent missions, it is now possible to study X-ray binaries in nearby galaxies. We present data on six luminous sources in the nearby spiral galaxy, M101, obtained with the Chandra ACIS-S. Of these, five appear to be similar to ultraluminous sources in other galaxies, while the brightest source, P098, shows some unique characteristics. We present our interpretation of the data in terms of an optically thick outflow, and discuss implications.Comment: Accepted for publication in Astrophysical Journal (16 pages including 4 figures

Accuracy of Standard Measures of Family Planning Service Quality: Findings from the Simulated Client Method

Despite widespread endorsement within the field of international family planning regarding the importance of quality of care as a reproductive right, the field has yet to develop validated data collection instruments to accurately assess quality in terms of its public health importance. This study, conducted among 19 higher volume public and private facilities in Kisumu, Kenya, used the simulated client method to test the validity of three standard data collection instruments included in large-scale facility surveys: provider interviews, client interviews, and observation of client-provider interactions. Results found low specificity and positive predictive values in each of the three instruments for a number of quality indicators, suggesting that quality of care may be overestimated by traditional methods. Revised approaches to measuring family planning service quality may be needed to ensure accurate assessment of programs and to better inform quality improvement interventions

Comparative effectiveness of dual-action versus single-action antidepressants for the treatment of depression in people living with HIV/AIDS

Background Depression is the most common psychiatric comorbidity among people living with HIV/AIDS (PLWHA). Little is known about the comparative effectiveness between different types of antidepressants used to treat depression in this population. We compared the effectiveness of dual-action and single-action antidepressants in PLWHA for achieving remission from depression. Methods We used data from the Centers for AIDS Research Network of Integrated Clinic Systems to identify 1175 new user dual-action or single-action antidepressant treatment episodes occurring from 2005 to 2014 for PLWHA diagnosed with depression. The primary outcome was remission from depression defined as a Patient Health Questionnaire-9 (PHQ-9) score <5. Mean difference in PHQ-9 depressive symptom severity was a secondary outcome. The main approach was an intent-to-treat (ITT) evaluation complemented with a per protocol (PP) sensitivity analysis. Generalized linear models were fitted to estimate treatment effects. Results In ITT analysis, 32% of the episodes ended in remission for both dual-action and single-action antidepressants. The odds ratio (OR) of remission was 1.02 (95%CI=0.63,1.67). In PP analysis, 40% of dual-action episodes ended in remission compared to 32% in single-action episodes. Dual-action episodes had 1.33 times the odds of remission (95%CI=0.55,3.21), however the result was not statistically significant. Non-significant differences were also observed for depressive symptom severity. Limitations Missing data was common but was addressed with inverse probability weights. Conclusions Results suggest that single-action and dual-action antidepressants are equally effective in PLWHA. Remission was uncommon highlighting the need to identify health service delivery strategies that aid HIV providers in achieving full remission of their patients’ depression

Chandra X-ray Sources in M101

A deep (98.2 ks) Chandra Cycle-1 observation has revealed a wealth of discrete X-ray sources as well as diffuse emission in the nearby face-on spiral galaxy M101. From this rich dataset we have created a catalog of the 110 sources from the S3 chip detected with a significance of >3 sigma, corresponding to a flux of ~1.0E-16 ergs/cm/cm/s and a luminosity of 1.0E36 ergs/s for a distance to M101 of 7.2 Mpc. The sources display a distinct correlation with the spiral arms and include a variety of X-ray binaries, supersoft sources, supernova remnants, and other objects of which only ~27 are likely to be background sources. There are only a few sources in the interarm regions, and most of these have X-ray colors consistent with that of background AGNs. The derived log N-log S relation for the sources in M101 (background subtracted) has a slope of -0.80+/-0.05 over the range of 1.0E36 - 1.0E38 ergs/s. The nucleus is resolved into 2 nearly identical X-ray sources, each with a 0.5-2.0 keV flux of 4.0E37 ergs/s. One of these sources coincides with the optical nucleus, and the other coincides with a cluster of stars 110 pc to the south.Comment: 39 pages including 13 figures and 4 tables; ApJ, in pres