Influence of root width and dentin wall thickness evaluated by endoscopy upon the outcome of periapical surgery. A cohort study

Background: An analysis was made of the correlation between root width, the thickness of the remaining dentinal wall as determined by endoscopy, and the outcome of periapical surgery. Material and Methods: A retrospective cohort study was carried out involving patients subjected to periapical surgery between 2017 and 2019 at the University of Valencia (Valencia, Spain). One year after surgery, cone-beam computed tomography (CBCT) was used to evaluate healing against the preoperative volumes. The maximum root width was measured on the postoperative CBCT scan at the apical section of the treated root. This measurement was transferred to the intraoperative endoscopic image, where the minimum root width, peripheral dentin thickness, and minimum dentin thickness were recorded. Root measurements, and the position (maxillary or mandibular) and type of tooth (roots of incisors, canines, premolars or molars) were further correlated to periapical surgery outcome. Results: A total of 51 patients, comprising 52 teeth and 62 roots, were included in the study. The mean measurements were: maximum root width (4.13 +/- 0.84 mm), minimum root width (2.46 +/- 0.72 mm), peripheral dentin thickness (0.77 +/- 0.2 mm) and minimum dentin thickness (0.4 +/- 0.2 mm). The success rate was 82.2%. Premolar roots showed a greater minimum dentin thickness (0.58 +/- 0.25 mm) ( p<0.003) than incisor roots. No significant association was found between the different measurements and the healing rate at one year, though the roots that failed to heal showed smaller minimum dentin thickness values than the roots that healed correctly. The position and type of tooth did not influence healing outcome. Conclusions: The root width and thickness of the remaining dentin wall did not significantly influence healing. However, the roots that failed to heal showed smaller minimum dentin thickness values than the roots that healed correctly

Impact of abutment geometry on early implant marginal bone loss. A double-blind, randomized, 6-month clinical trial

Objectives The objective of this study was to analyze the impact of the abutment width on early marginal bone loss (MBL). Material and Methods A balanced, randomized, double-blind clinical trial with two parallel experimental arms was conducted without a control group. The arms were “cylindrical” abutment and “concave” abutment. Eighty hexagonal internal connection implants, each with a diameter of 4 × 10 mm, were placed in healed mature bone. The main variable was the peri-implant tissue stability, which was measured as MBL at 8 weeks and 6 months. Results The final sample consisted of 77 implants that were placed in 25 patients. 38 (49.4%) were placed using the cylindrical abutment, and the other 39 (50.6%) were placed using the concave abutment. The early global MBL of −0.6 ± 0.7 mm in the cylindrical abutment group was significantly higher than it was in the concave abutment group, in which the early global MBL was −0.4 ± 0.6 mm (p = .030). The estimated effect size (ES) was negative for the cylindrical abutment (ES = −1.3730, CI −2.5919 to −0.1327; t-value = −2.4893; p = .0139), therefore implying a loss of mean bone level, and it was positive for the concave abutment (ES = 2.8231; CI: 1.4379 to 4.2083; t-value = 4.0957; p = .0002), therefore implying an increase in the average bone level. Conclusions The concave abutments presented significantly less early MBL at 6 months post-loading than classical cylindrical abutments did

Microbial Colonization of the Peri-Implant Sulcus and Implant Connection of Implants Restored With Cemented Versus Screw-Retained Superstructures: A Cross-Sectional Study

Background: The aim of this study is to investigate peri-implant and intraconnection microflora of healthy implants restored with cemented and screwed superstructures. Methods: Patients with two to three implants restored with cemented or screwed restorations and 5 years of follow-up were recruited. Samples were taken from peri-implant sulci, adjacent teeth, and the inner portion of connections. Prevalence of positive sites and bacterial loads for 10 microorganisms were obtained with quantitative real-time polymerase chain reaction. Implant connection permeability to the studied microorganisms was estimated using a standardized bacterial contamination index. Statistical analysis was performed using a generalized estimating equations model, Wald x2 test, and the least significant difference test. Results: The final sample consisted of 18 patients (55 implants) in the cemented group and 22 patients (46 implants) in the screw-retained group. Regarding prevalence of positive sites, significant differences between groups were only found for Tannerella forsythia, which was 8.7 times more frequent at peri-implant sulci of cemented than screw-retained prostheses. Bacterial loads of Porphyromonas gingivalis, T. forsythia, Parvimonas micra, and total bacterial load were significantly higher at peri-implant sulci for the cemented group; at the inner portion of connections, values were significantly higher for P. micra and Fusobacterium nucleatum for the screw-retained group. Contamination index values demonstrated higher permeability to most microbes in the cemented group. Conclusions: Internal implant surfaces were microbiologically contaminated for both cemented and screw-retained superstructures. Differences were found between the two methods of prosthetic retention: the cemented group presented significantly higher bacterial loads in the peri-implant sulcus but significantly lower bacterial loads at the inner portion of the implant connection

Culturomic and quantitative real- time- polymerase chain reaction analyses for early contamination of abutments with different surfaces: A randomized clinical trial

BackgroundRough and/or plasma- activated abutments seem to be able to increase soft tissue adhesion and stability; however, limited evidence is available about bacterial contamination differences.PurposeThe aim was to investigate the oral microbiota on four dental abutments with different surfaces by quantitative real- time polymerase chain reaction (qRT- PCR) and culturomic approach.MethodsForty patients needing a single implant rehabilitation were involved in the study. Forty healing abutments, especially designed for the study, were divided into four groups according to the surface topography (1. machined [MAC], 2. machined plasma of argon treated [plasmaMAC], 3. ultrathin threaded microsurface [UTM], 4. UTM plasma of argon treated [Plasma UTM]). Random assignment was performed according to predefined randomization tables. All patients underwent surgical intervention for implant and contextual healing abutment positioning. After 2 months of healing, a sterile cotton swab was used for microbiological sampling for culturomics, while sterile paper points inserted into the sulcus were used for qRT- PCR.ResultsAt the end of the study, 36 patients completed all procedures and a total of 36 abutments (9 per group) were analyzed. qRT- PCR retrieved data for 23 bacterial species whereas culturomics revealed the presence of 74 different bacteria, most of them not routinely included into oral cavity microbiological kits of analysis or never found before in the oral microenvironment. No statistically significant differences emerged analyzing the four different surfaces (p =- 0.053). On the contrary, higher total and specific bacterial counts were detected in the plasma- treated surfaces compared to the untreated ones (p =- 0.021).ConclusionsAbutments with different topographies and surface treatments resulted contaminated by similar oral bacterial flora. Abutments with moderately rough surface were not associated with a greater bacterial adhesion compared to machined ones. Conversely, more bacteria were found around plasma- treated abutments. Furthermore, data reported suggested to include new species not previously sought in the routine analyses of the oral bacterial microflora.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/169240/1/cid13028_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/169240/2/cid13028.pd

Culturomic and quantitative real‐time polymerase chain reaction analyses for early contamination of abutments with different surfaces: A randomized clinical trial

none9simixedCanullo, Luigi; Masucci, Luca; Quaranta, Gianluca; Patini, Romeo; Caponio, Vito Carlo Alberto; Pesce, Paolo; Ravidà, Andrea; Penarrocha‐Oltra, David; Penarrocha‐Diago, MiguelCanullo, Luigi; Masucci, Luca; Quaranta, Gianluca; Patini, Romeo; Caponio, Vito Carlo Alberto; Pesce, Paolo; Ravidà, Andrea; Penarrocha‐oltra, David; Penarrocha‐diago, Migue

Prognostic Factors after Endodontic Microsurgery: A Retrospective Study of 111 Cases with 5 to 9 Years of Follow-up

Introduction: A study was performed of the healing rate of teeth subjected to endodontic microsurgery after a minimum follow-up of 5 years with an analysis of the influence of different pre- and postoperative factors on the outcome. Methods: This was a retrospective study of patients subjected to endodontic microsurgery with the use of mineral trioxide aggregate (MTA) for retrograde filling between January 2011 and December 2015. In patients with multiple treated teeth, only 1 random tooth was selected for the statistical analysis. Clinical and radiographic parameters were used to assess healing. Simple binary logistic regression models were used to analyze the influence of patient age and sex, the type of tooth, prior radiographic lesion size, the presence of a post, the type of restoration, and the apical extent of prior filling of the root canal on the endodontic microsurgery success rate. Two calibrated observers evaluated the periapical radiographs on an independent basis. Results: A total of 111 patients (63.1% women and 36.9% men) were included in the study. Of the 111 teeth analyzed, 90 were classified as healed (mean healing rate = 81.1%). Patient age and sex, the presence of a post, the type of tooth, the type of restoration, and the apical extent of prior filling of the root canal had no significant impact on the outcome. Large lesions (>5 mm) were associated with a lower healing rate than smaller lesions, although the difference was not significant. Anterior teeth had a significantly higher healing rate (93.8% maxillary and 100% mandibular) than molars (70.8% maxillary and 57.1% mandibular) (P < .05). The differences between the anterior teeth and the molars were statistically significant. Conclusions: The mean healing rate of teeth subjected to endodontic microsurgery was 81% after 5-9 years of follow-up. The success rate was lower for upper and lower molars than for teeth in the anterior zone, although the sample was small and further studies are needed to establish whether the type of tooth influences the treatment outcome.Odontologí