Using geographical and taxonomic metadata to set priorities in specimen digitization

Digitizing the information carried by specimens in natural history collections is a key endeavor providing falsifiable information about past and present biodiversity on a global scale, for application in a variety of research fields far beyond the current application in biosystematics. Existing digitization efforts are driven by individual institutional necessities and are not coordinated on a global scale. This led to an over-all information resource that is patchy in taxonomic and geographic coverage as well as in quality. Digitizing all specimens is not an achievable aim at present, so that priorities need to be set. Most biodiversity studies are both taxonomically and geographically restricted, but access to non-digitized collection information is almost exclusively by taxon name. Creating a “Geotaxonomic Index” providing metadata on the number of specimens from a specific geographic region belonging to a specific higher taxonomic category may provide a means to attract the attention of researchers and governments towards relevant non-digitized holdings of the collections and set priorities for their digitization according to the needs of information users outside the taxonomic community

Biparental inbreeding depression, genetic relatedness and progeny vigor in a wind-pollinated treeline species in Argentina

Spatially restricted gene flow and resulting spatial genetic structure are generally considered as being the primary controlling factors in the dynamics of biparental inbreeding depression in a wide range of plant species. However, wind-pollinated angiosperm trees have not been adequately studied in this respect. The present study analyses the relationships between parental genetic similarity, outcrossing distances and progeny vigour in Polylepis australis (Rosaceae), a wind-pollinated treeline species endemic to Argentina. We investigated whether spatial genetic structuring occurs in anthropogenically fragmented P. australis woodlands of the Córdoba Mountains. We also performed a controlled crossing experiment using pollen collected from different distances. Genetic variability (using RAPD-PCR) and vigour (survival and N metabolism capacity) of the resulting progeny were contrasted with progeny from unmanipulated flowers. We found a continuous decrease in parental genetic similarity with spatial distance among mates and an increase in genetic variability, survival and N metabolism capacity in the progeny produced from pollen at increasing distances. However, our further results suggest fragment connectivity in P. australis through effective long-distance pollen-mediated gene flow with no effective inbreeding depression problems observed under present day conditions.Fil: Seltmann, Peggy. Martin-Luther-University Halle-Wittenberg; AlemaniaFil: Hensen, Isabell. Martin-Luther-University Halle-Wittenberg; AlemaniaFil: Renison, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Wesche, Karsten. University of Göttingen; AlemaniaFil: Ploch, Sebastián. University of Hohenheim; AlemaniaFil: Rondan Dueñas, Juan. Universidad Nacional de Córdoba; ArgentinaFil: Cocucci, Andrea Aristides. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Jung, Klaus. Helmholtz Centre for Environmental Research-UFZ; Alemani

Variation of seed mass and its effects on germination in Polylepis australis: Implications for seed collection

South American Polylepis mountain forests are recognised as being one of the most endangered forest ecosystems in the world. Reforestation measures have been strongly recommended but may be hampered due to the very low seed germination reported for several Polylepis species. In order to facilitate reforestation we analysed the influence of seed mass on germination probability for Polylepis australis seeds in the Córdoba mountains (central Argentina). We collected seeds from 43 trees distributed throughout five woodland fragments located within two regions differing in size, topographical position, and altitude (1,900 m a.s.l. and 2,200 m a.s.l.). Seeds of Polylepis australis exhibited a great variation in terms of mass and percent seed germination among individual trees and among geographical regions. The results of logistic regression showed that germination probability was highly correlated with seed mass. However, the explained deviance significantly increased by including the region, the woodland fragment and especially the individual tree in addition to seed mass in the regression models. We conclude that selecting seeds on the basis of mass is an appropriate way to enhance germination prospects for reforestation projects. However, no absolute mass values are applicable in this context as the highest germination probabilities were reached at varying seed mass values depending on geographical region, woodland fragment or individual tree. We suggest collecting the relatively heaviest available seeds, even though the absolute seed mass may be low.Fil: Seltmann, Peggy. GardenMartin-Luther-University. Institute of Geobotany and Botanical; AlemaniaFil: Leyer, Ilona. Conservation Biology Philipps-University Marburg. Department of Biology; AlemaniaFil: Renison, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Hensen, Isabell. GardenMartin-Luther-University. Institute of Geobotany and Botanical; Alemani

Mating system, outcrossing distance effects and pollen availability in the wind-pollinated treeline species Polylepis australis BITT. (Rosaceae)

Fragmentation may negatively affect plant fitness through pollen limitation and increased levels of inbreeding. Effects of fragmentation may vary with regard to life form and breeding system, and few studies exist for wind- pollinated trees. We examined the effects of hand-selfing, varying outcrossing distances and pollen addition on seed mass and germination rate of Polylepis australis BITT. (Rosaceae), a wind-pollinated treeline species endemic to Argentina. We also investigated pollen germination on the stigma and pollen tube growth to determine compatibility resulting from selfing and outcrossing. Selfing reduced seed germination rates with significant differences between open pollination and outcrosses at 30km. In addition, we found a tendency for pollen germination and pollen tube growth to decrease following selfing. Between-fragment crosses resulted in a trend of higher reproductive out put than within- fragment crosses, whereas values were similar between open pollination and between-fragment crosses. Pollen addition did not increase reproductive success neither in small nor in larger fragments. Our results suggest that highly isolated P. australis forests have a potential for inbreeding depression through selfing and within-fragment crosses. However, the results also indicate that pollen flow between P. australis forest fragments is still effective at the current fragmentation level, counteracting negative effects on seed quality resulting from reproductive isolation.Durch Habitatzerstörung und Fragmentierung isolierte und kleine Populationen leiden häufig unter erhöhter Inzuchtdepression und Pollenlimitierung, deren Ausmaß jedoch in Abhängigkeit von Lebensform und Fortpflanzungssystem variiert. In der vorliegenden Studie untersuchten wir den Einfluss von Hand-Selbstbestäubung, verschiedenen Kreuzungsdistanzen bis zu 30 km und Pollenzugabe auf Samenmasse und -keimung von Polylepis australis (Rosaceae), einer windbestäubten, in Argentinien endemischen Baumart der oberen Waldgrenze. Darüber hinaus analysierten wir den Einfluss von Selbst- und Fremdbestäubung auf Pollenkeimung und Pollenschlauchwachstum. Selbstbestäubung führte zu signifikant geringeren Keimraten im Vergleich zu natürlicher Bestäubung und Fremdbestäubung mit Pollen aus >30 km Distanz. Auch fanden wir eine Tendenz zur Reduktion von Pollenkeimung und Pollenschlauchwachstum nach Selbstbestäubung. Kreuzungen zwischen Fragmenten führten zu höherem Reproduktionserfolg als Kreuzungen innerhalb von Fragmenten, während der Reproduktionserfolg aus natürlicher Bestäubung und aus Kreuzungen zwischen Fragmenten vergleichbare Werte aufwies. Weder in kleinen noch in großen Populationen führte die Zugabe von Pollen zu einer Erhöhung des Reproduktionserfolges. Unsere Ergebnisse machen deutlich, dass zwar in stark isolierten P. australis Wäldern die Gefahr für Inzuchtdepression besteht, der nach wie vor effektive Pollen- und Genfluss zwischen Waldfragmenten von P. australis jedoch negativen Isolationseffekten entgegenwirkt.Fil: Seltmann, Peggy. Martin-Luther-University Halle-Wittenberg; AlemaniaFil: Cocucci, Andrea Aristides. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Renison, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Cierjacks, Arne. Martin-Luther-University Halle-Wittenberg; AlemaniaFil: Hensen, Isabell. Martin-Luther-University Halle-Wittenberg; Alemani

Fragment size, pollination efficiency and reproductive success in natural populations of wind-pollinated Polylepis australis (Rosaceae) trees

It is well known that habitat fragmentation is likely to negatively affect the reproductive success of a species. Despite this fact, studies on the effects of fragmentation on reproductive success in combination with effects on natural pollination of wind-pollinated tree species are very rare. In this study, we analyzed the relationships between fragment size, components of pollination efficiency, reproductive success and progeny vigour of the highly fragmented populations of the wind-pollinated treeline species Polylepis australis (Rosaceae) in Argentina. We conducted our study in the high mountains of Córdoba, comparing pollination efficiency and reproduction between four fragment sizes. Most of our results indicate that P. australis is currently insensitive to fragmentation. However, our study revealed also a linear increase in leaf area and biomass of 40-day-old seedlings with increasing fragment size. Inbreeding problems through fragmentation as well as decreased habitat quality in small fragments are discussed as possible causes for the impaired progeny vigour.Fil: Seltmann, Peggy. Martin-Luther-University; AlemaniaFil: Renison, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Martin-Luther-University; AlemaniaFil: Cocucci, Andrea Aristides. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Hensen, Isabell. Martin-Luther-University; AlemaniaFil: Jung, Klaus. Centre for Environmental Research Leipzig-Halle; Alemani