Nutritional behavior and motives of college students for the choice of traditional food in the Republic of Serbia

The aim of this study was to investigate the eating behavior of college students and the reasons for consuming traditional food and to compare the motives for choosing traditional food with the research conducted in 6 European countries. This research was conducted using anonymous online questionnaires. The majority of surveyed students are physically active (75%) and live with their families (57.0%), which can have a positive impact on their diet and a lower level of consumption of "fast-food" (17.5%). Respondents have bad habits in terms of consuming cigarettes (65.0%), alcohol (73.0%) and energy drinks (75.0%). Most students consume all regular meals (73.0%). Based on the Body Mass Index (BMI) of respondents, they belong to the categories: underweight (12%), normal weight (34%), pre-weight (17%), obese (37%); however, 55.0% believed to have "ideal weight". The reasons for choose particular food are: it is not genetically modified, it tastes good, it is nutritious, it makes them happy, it was produced/packaged in an environmentally friendly and ethical way, while the price of food is not important. Connection with family (81%) and food being tasty (54%) are the main reasons for consuming traditional food. When buying traditional food, respondents (59%) generally do not check the declaration on the product. These results indicate the need to educate students about the harmfulness of cigarettes, alcoholic and energy drinks, the importance of BMI and declaration on the product. Comparing obtained results with the results in 6 European countries it can be noticed that the answers of the respondents in Serbia were the most similar to those obtained in Poland

Supplementary material for the article: Jeremić, M.; Pešić, M.; Dinić, J.; Banković, J.; Novakovićc, I.; Šegan, D.; Sladić, D. Simple Avarone Mimetics as Selective Agents against Multidrug Resistant Cancer Cells. European Journal of Medicinal Chemistry 2016, 118, 107–120. https://doi.org/10.1016/j.ejmech.2016.04.011

Supplementary material for: [https://doi.org/10.1016/j.ejmech.2016.04.011]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2256

Ocena stopnia zaawansowania miażdżycy u dzieci i młodzieży z cukrzycą typu 1

Introduction: To evaluate the degree of atherosclerosis in children and adolescents with type 1 diabetes and its correlation with risk factors, traditional and other, such as anti-oxidative capacity of circulating blood and level of lipid peroxidation. Material and methods: Forty children and adolescents with type 1 diabetes with mean age 13.7 years were compared with 20 age- and sex-matched healthy control subjects. Association of carotid artery intima-media thickness (cIMT) with different risk factors measured in children with type 1 diabetes was evaluated. Results: Mean carotid IMT was higher in subjects with diabetes (p < 0.01) and was strongly associated with total cholesterol with an odds ratio of 4.08 (p = 0.016), LDL-cholesterol with an odds ratio of 2.78 (p = 0.037), length of disease with an odds ratio of 1.87 (p = 0.007) and positive family history (first- and second-degree relatives) of diabetes and early CVD (heart attack and/or stroke before the age of 60 years) with an odds ratio of 6.8 (p = 0.007). Conclusions: We found significantly increased cIMT in the diabetic patients compared to the healthy control subjects. Risk factors for the development of atherosclerosis included higher total and LDL-cholesterol, higher systolic blood pressure, positive family history of diabetes and early CVD and longer diabetes duration. In spite of the documented increased oxidative stress, we failed to establish a correlation between the oxidative stress parameters and cIMT values. (Endokrynol Pol 2012; 63 (6): 414&#8211;419)Wstęp: Ocena stopnia zaawansowania miażdżycy u dzieci i młodzieży z cukrzycą typu 1 oraz jego korelacja z czynnikami ryzyka, tradycyjnymi i innymi, na przykład potencjałem antyoksydacyjnym krwi krążącej i poziomem peroksydacji lipidów. Materiał i metody: Porównano 40 dzieci i nastolatków z cukrzycą typu 1, średnia wieku 13,7 roku, z 20 zdrowymi osobnikami stanowiącymi grupę kontrolną odpowiednio dobranymi pod względem wieku i płci. Oceniano związek pomiędzy grubością kompleksu błony wewnętrznej i środkowej tętnic szyjnych (cIMT) a różnymi czynnikami ryzyka ocenianymi ilościowo u dzieci z cukrzycą typu 1. Wyniki: Średnia wartość cIMT była większa u pacjentów z cukrzycą (p < 0,01) i wykazywała silny związek ze stężeniem cholesterolu całkowitego [iloraz szans (OR) 4,08; p = 0,016], cholesterolem frakcji LDL (OR 2,77; p = 0,037), czasem trwania choroby (OR 1,87; p = 0,007) i dodatnim wywiadem rodzinnym (krewni pierwszego i drugiego stopnia) w kierunku cukrzycy i wczesnego występowania chorób układu krążenia (zawału serca i/lub udaru mózgu przed 60. rokiem życia) (OR 6,8; p = 0,007). Wnioski: Stwierdzono znamiennie podwyższoną wartość cIMT u pacjentów z cukrzycą w porównaniu ze zdrowymi osobnikami z grupy kontrolnej. Czynniki ryzyka rozwoju miażdżycy obejmowały podwyższone stężenie cholesterolu całkowitego i cholesterolu frakcji LDL, podwyższone skurczowe ciśnienie tętnicze, dodatni wywiad rodzinny w kierunku cukrzycy i wczesnego występowania chorób układu krążenia oraz dłuższy czas trwania cukrzycy. Pomimo udokumentowanego wzmożenia stresu oksydacyjnego nie udało się ustalić korelacji pomiędzy parametrami stresu oksydacyjnego a wartościami cIMT. (Endokrynol Pol 2012; 63 (6): 414&#8211;419

Fenotyp typu &#8222;talia hipertriglicerydemiczna&#8220; i zespół metaboliczny określany na podstawie różnych kryteriów oraz zależności między tymi zaburzeniami a kontrolą stężeń lipidów i glikemii u chorych na cukrzycę typu 2

Background: Metabolic syndrome (MetS) describes clustering of obesity, dyslipidemia, hyperglycemia and hypertension and increases risk for cardiovascular disease and type 2 diabetes. The &#8216;hypertriglyceridemic waist&#8217; phenotype (HTGW) represents a simple approach to identifying individuals with increased risk. The aim of the study was to determine the prevalence of HTGW and MetS in type 2 diabetic patients, and to examine their relation to lipids and blood glucose control. Material and methods: 300 type 2 diabetic patients were analysed, and their history of diabetes, anthropometric measures, measurements of blood pressure (BP), lipids and glycemic control parameters were taken. Results: In type 2 diabetic patients, the prevalence of MetS was 71.0% by the AHA/NHLBI definition and 75.33% by the IDF definition. The prevalence was 62.58% and 66.45% in men, and 80% and 84.83% in women by the same definitions, respectively. There were 41.33% of patients with HTGW (42.76% among women and 40% among men). There were statistically significant differences of age, fasting plasma glucose (FPG) and postprandial glucose (PPG) in women with and without MetS according to both definitions, and of total and LDL cholesterol with and without MetS according to AHA/NHLBI (but not IDF). In men, there were statistically significant differences of total cholesterol and of HbA1c with and without MetS according to AHA/NHLBI (but not IDF). Women with HTGW had higher levels of total and LDL cholesterol, systolic and diastolic BP. Men with HTGW had higher levels of total cholesterol, diastolic BP, HbA1c, FPG and PPG. Conclusions: Determining MetS or HTGW helps identify those with increased cardiovascular risk. (Pol J Endocrinol 2011; 62 (4): 316&#8211;323)Wstęp: Zespół metaboliczny (MetS) obejmujący otyłość, dyslipidemię, hiperglikemię i nadciśnienie tętnicze zwiększa ryzyko chorób sercowo-naczyniowych i cukrzycy typu 2. Określanie fenotypu &#8222;talii hipertriglicemicznej&#8221; (HTGW) jest prostą metodą identyfikowania chorych z grupy wysokiego ryzyka. Celem badania było ustalenie częstości HTGW i MetS u chorych na cukrzycę typu 2 oraz ocena zależności miedzy tymi zaburzeniami a kontrolą stężeń lipidów i glikemii. Materiał i metody: Do badania włączono 300 chorych na cukrzycę typu 2 i przeanalizowano dane dotyczące przebiegu cukrzycy, parametrów antropometrycznych, wartości ciśnienia tętniczego, stężeń lipidów i kontroli glikemii. Wyniki: U chorych na cukrzycę typu 2 kryteria MetS według definicji AHA/NHLBI spełniało71,0%, a kryteria IDF &#8212; 75,33%; odsetek chorych z MetS wynosił wśród mężczyzn odpowiednio 62,58% i 66,45%, a wśród kobiet 80% i 84,83%. U 41,33% chorych stwierdzono cechy HTGW, 42,76% tej grupy stanowiły kobiety, a 40% mężczyźni. U kobiet wykazano istotne statystycznie różnice w zakresie wieku, glikemii na czczo (FPG) i glikemii poposiłkowej (PPG) między grupami z MetS i bez niego, rozpoznanym na podstawie obu definicji, natomiast w zakresie stężenia cholesterolu całkowitego i frakcji LDL różniły się one tylko między grupami z MetS i bez niego wydzielonymi na podstawie definicji AHA/NHLBI (a nie na podstawie kryteriów IDF). U mężczyzn wykazano statystycznie istotne różnice stężeń cholesterolu całkowitego HbA1c między grupami z MetS i bez niego określonym według AHA/NHLBI (ale nie według IDF). U kobiet z HTGW stwierdzono wyższe stężenia cholesterolu całkowitego i cholesterolu frakcji LDL oraz wyższe wartości ciśnienia skurczowego i rozkurczowego. U mężczyzn z HTGW odnotowano wyższe wartości stężeń cholesterolu całkowitego, rozkurczowego ciśnienia tętniczego, HbA1c, FPG i PPG. Wnioski: Rozpoznanie MetS lub HTGW pozwala zidentyfikować osoby obciążone zwiększonym ryzykiem sercowo-naczyniowym. (Endokrynol Pol 2011; 62 (4): 316&#8211;323

Supplementary data for the article: Dinić, J.; Novaković, M.; Podolski-Renić, A.; Stojković, S.; Mandić, B.; Tešević, V.; Vajs, V.; Isaković, A.; Pešić, M. Antioxidative Activity of Diarylheptanoids from the Bark of Black Alder (Alnus Glutinosa) and Their Interaction with Anticancer Drugs. Planta Medica 2014, 80 (13), 1088–1096. https://doi.org/10.1055/s-0034-1382993

Supplementary material for: [https://doi.org/10.1055/s-0034-1382993]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1845

Supplementary data for the article: Novaković, M.; Pešić, M.; Trifunović, S.; Vučković, I.; Todorović, N.; Podolski-Renić, A.; Dinić, J.; Stojković, S.; Tešević, V.; Vajs, V.; et al. Diarylheptanoids from the Bark of Black Alder Inhibit the Growth of Sensitive and Multi-Drug Resistant Non-Small Cell Lung Carcinoma Cells. Phytochemistry 2014, 97, 46–54. https://doi.org/10.1016/j.phytochem.2013.11.001

Supplementary material for: [https://doi.org/10.1016/j.phytochem.2013.11.001]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1492

Supplementary material for the article: Jeremić, M.; Pešić, M.; Dinić, J.; Banković, J.; Novakovićc, I.; Šegan, D.; Sladić, D. Simple Avarone Mimetics as Selective Agents against Multidrug Resistant Cancer Cells. European Journal of Medicinal Chemistry 2016, 118, 107–120. https://doi.org/10.1016/j.ejmech.2016.04.011

Supplementary material for: [https://doi.org/10.1016/j.ejmech.2016.04.011]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2256

Supplementary material for the article: Dinić, J.; Novaković, M.; Podolski-Renić, A.; Vajs, V.; Tešević, V.; Isaković, A.; Pešić, M. Structural Differences in Diarylheptanoids Analogues from Alnus Viridis and Alnus Glutinosa Influence Their Activity and Selectivity towards Cancer Cells. Chemico-Biological Interactions 2016, 249, 36–45. https://doi.org/10.1016/j.cbi.2016.02.019

Supplementary material for: [https://doi.org/10.1016/j.cbi.2016.02.019]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2066

On optimal temozolomide scheduling for slowly growing glioblastomas

Background: Temozolomide (TMZ) is an oral alkylating agent active against gliomas with a favorable toxicity profile. It is part of the standard of care in the management of glioblastoma (GBM), and is commonly used in low-grade gliomas (LGG). In-silico mathematical models can potentially be used to personalize treatments and to accelerate the discovery of optimal drug delivery schemes. Methods: Agent-based mathematical models fed with either mouse or patient data were developed for the in-silico studies. The experimental test beds used to confirm the results were: mouse glioma models obtained by retroviral expression of EGFR-wt/EGFR-vIII in primary progenitors from p16/p19 ko mice and grown in-vitro and in-vivo in orthotopic allografts, and human GBM U251 cells immobilized in alginate microfibers. The patient data used to parametrize the model were obtained from the TCGA/TCIA databases and the TOG clinical study. Results: Slow-growth "virtual" murine GBMs benefited from increasing TMZ dose separation in-silico. In line with the simulation results, improved survival, reduced toxicity, lower expression of resistance factors, and reduction of the tumor mesenchymal component were observed in experimental models subject to long-cycle treatment, particularly in slowly growing tumors. Tissue analysis after long-cycle TMZ treatments revealed epigenetically driven changes in tumor phenotype, which could explain the reduction in GBM growth speed. In-silico trials provided support for implementation methods in human patients. Conclusions: In-silico simulations, in-vitro and in-vivo studies show that TMZ administration schedules with increased time between doses may reduce toxicity, delay the appearance of resistances and lead to survival benefits mediated by changes in the tumor phenotype in slowly-growing GBMs.This research was funded by the James S. Mc. Donnell Foundation (USA) 21st Century Science Initiative in Mathematical and Complex Systems Approaches for Brain Cancer (Collaborative award 220020560, doi:10.37717/220020560); Ministry of Education, Science and Technological Development, Republic of Serbia (ref. number 451-03-9/2021-14/200007); Ministerio de Ciencia e Innovación and FEDER funds, Spain (grant number PID2019-110895RB-I00, doi: 10.13039/501100011033 to VMP-G, and RTI2018-093596 and PI21CIII/00002 to PS-G); and Universidad de Castilla-La Mancha (grant number 2020-PREDUCLM-15634 to JJ-S).S